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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭彥彬 | |
| dc.contributor.author | Huan-Ching Chiang | en |
| dc.contributor.author | 江桓慶 | zh_TW |
| dc.date.accessioned | 2021-06-07T23:57:38Z | - |
| dc.date.copyright | 2013-09-24 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-17 | |
| dc.identifier.citation | Angelopoulos AP, Goaz PW (1972). Incidence of diphenylhydantoin gingival hyperplasia. Oral surgery, oral medicine, and oral pathology 34(6):898-906.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17130 | - |
| dc.description.abstract | 緒論：藥物引起的牙齦過度生長常因為無法換藥而難以從根本改善，因此手術切除術成為較常使用的方法。但手術後的復發率高，除了藥物本身的影響，傷口處會有大量的凝血酶聚集，除了幫助止血，傷口癒合外，也可能在此好發牙齦腫大的族群中，造成過度的細胞間質堆積，而造成再發率居高不下。研究目的：利用人類牙齦纖維母細胞，以凝血酶誘導，觀察肌纖維母細胞纖維化的標記物α型平滑肌肌動蛋白(α-SMA)的表現，並尋找可抑制此反應的抑制劑。材料與方法：本實驗利用人類牙齦纖維母細胞，以凝血酶處理細胞，以西方墨點法檢測α-SMA，並以不同的抑制劑(PP2, EGCG, lovastatin, and curcumin)合併凝血酶處理細胞，觀察α-SMA表現量的變化。結果：凝血酶會誘導人類牙齦纖維母細胞中α-SMA的表現，且使用的Src family抑制劑PP2及天然抑制劑 (EGCG and curcumin)及降血脂藥物(lovastatin)可有效抑制凝血酶所誘導的α-SMA表現。結論：凝血酶會經由Src誘導人類牙齦纖維母細胞中α-SMA的表現，EGCG、lovastatin和curcumin可抑制其表現。 | zh_TW |
| dc.description.abstract | Introduction: The best way to treat drug-induced gingival overgrowth (DIGO) is changing medication. But we often encounter the situation that we can’t change the medication. In the situation, surgical intervention by gingivectomy or flap operation is the resolution of DIGO. However, the recurrence rate is high even under intensive follow up and good oral hyginene. Beside the effect of the medication, activation of thrombin around the wound, in addition to the function of hemostasis and wound healing, may also cause overdeposition of the extracellular matrix and the high recurrance rate of DIGO.
Purpose: To observe the expression of alpha smooth muscle actin (α-SMA), marker of myofibroblast and fibrotic activity, after the treatment of thrombin in human gingival fibroblasts. Then to try to find the inhibitor to inhibit this reaction. Material and method: Using Western blotting technique for analysis of α-SMA after treatment of thrombin in human gingival fibroblasts. Then after additional pre-treatment of the inhibitors (PP2, EGCG, lovastatin and curcumin), to observe the expression of α-SMA induced by thrombin. Result: Thrombin induces the expression of α-SMA. And the Src family inhibitor(PP2), natural inhibitors (EGCG and curcumin) and drug for cholesterol-lowering (lovastatin) can inhibit the expression of α-SMA induced by thrombin. Conclusion: Thrombin induced the expression of α-SMA through Src in human gingival fibroblasts. And it can be inhibited by EGCG, lovastatin and curcumin. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T23:57:38Z (GMT). No. of bitstreams: 1 ntu-102-R99422030-1.pdf: 1911535 bytes, checksum: 888241ae62b8d41aad73f4130e12a2ca (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 誌謝................................................................. I
摘要................................................................. II ABSTRACT.......................................................III 目錄.................................................................IV 圖目錄.............................................................VII 緒論..........................................................1 第一節 牙齦過度生長(Gingival overgrowth; GO)...............................................1 1-1 藥物誘發之牙齦過度生長 (Drug-induced gingival overgrowth; DIGO)..........................................................1 1-2 遺傳性牙齦纖維瘤病 (Hereditary gingival fibromatosis; HGF) ..........................................................2 第二節 藥物誘發之牙齦過度生長的可能治病治病機轉..........................................................2 2-1 細胞外基質代謝異常..........................................................2 2-2 細胞激素平衡異常..........................................................3 第三節 遺傳性牙齦纖維瘤病(HGF)的可能致病機轉..........................................................3 第四節 牙齦過度生長的治療策略..........................................................4 4-1 治療藥物誘發之牙齦過度生長(DIGO)..........................................................4 4-2 治療遺傳性牙齦纖維瘤病..........................................................6 第五節 傷口癒合(wound healing).......................................................... 6 第六節 凝血酶(thrombin)..........................................................7 6-1 凝血酶的簡介..........................................................7 6-2 蛋白酶活化受器(protease-activated receptors; PARs)..........................................................7 6-3 凝血酶與纖維化..........................................................8 第七節 肌纖維母細胞(myofibroblast)..........................................................9 第八節 α型平滑肌肌動蛋白(α-smooth muscle actins; α-SMA)..........................................................9 8-1 α-SMA的簡介..........................................................10 8-2 α-SMA在細胞中的表現..........................................................10 8-3 α-SMA與肌纖維母細胞 ..........................................................11 第九節 兒茶素EGCG..........................................................11 第十節 Lovastatin..........................................................12 第十一節 薑黃素 ( Curcumin )..........................................................13 研究目的..........................................................14 實驗材料與方法..........................................................15 第一節 細胞株與細胞培養..........................................................15 第二節 藥物處理 ..........................................................15 2-1 凝血酶..........................................................15 2-2 Inhibitor使用資料..........................................................16 第三節 西方墨點法 (Wester blotting)..........................................................16 3-1 蛋白質萃取 ..........................................................16 3-2 蛋白質濃度測定 (Total protein analysis)..........................................................16 3-3 膠體配置與電泳分析..........................................................17 3-4 蛋白質轉漬 (Protein transfer)..........................................................17 3-5 抗體使用以及顯影..........................................................18 3-6統計分析..........................................................18 結果 ..........................................................19 一、凝血酶可以誘導牙齦纖維母細胞(GF)α-SMA的表現..........................................................19 二、Src抑制劑PP2能夠抑制凝血酶誘導之牙齦纖維母細胞中α-SMA的表現 ..........................................................19 三、Src抑制劑PP2能夠抑制凝血酶誘導之牙齦纖維母細胞中CTGF的表現..........................................................19 四、EGCG、Lovastatin和Curcumin能夠抑制凝血酶誘導之牙齦纖維母細胞中α-SMA的表現..........................................................19 討論..........................................................21 結論..........................................................23 圖表..........................................................24 圖一、凝血酶在不同濃度下誘導牙齦纖維母細胞α-SMA的表現(A)及定量(B)..........................................................24 圖二、凝血酶在不同時間點下誘導牙齦纖維母細胞α-SMA的表現(A)及定量(B)..........................................................25 圖三、Src family抑制劑(PP2)以及ET1抑制劑(PD145065)兩種抑制劑對凝血酶誘導牙齦纖維母細胞表現α-SMA的影響(A)與定量(B)。..........................................................26 圖四、Src family抑制劑(PP2)以及ET1抑制劑(PD145065)兩種抑制劑對凝血酶誘導牙齦纖維母細胞表現CTGF的影響(A)與定量(B)。..........................................................27 圖五、不同抑制劑對凝血酶誘導牙齦纖維母細胞α-SMA的影響(A)及定量(B)..........................................................28 圖六、不同濃度的EGCG對凝血酶誘導牙齦纖維母細胞α-SMA表現的影響(A)及定量(B) ..........................................................29 圖七、不同濃度的Lovastatin對凝血酶誘導牙齦纖維母細胞α-SMA表現的影響(A)及定量(B) ..........................................................30 圖八、不同濃度的Curcumin對凝血酶誘導牙齦纖維母細胞α-SMA表現的影響(A)及定量(B) ..........................................................31 參考文獻..........................................................32 | |
| dc.language.iso | zh-TW | |
| dc.title | 凝血酶經由經由Src誘導人類牙齦纖維母細胞表現α-SMA | zh_TW |
| dc.title | Thrombin Induced Expression of α-SMA through Src in Human Gingival Fibroblast | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 周涵怡,張瑞青 | |
| dc.subject.keyword | 藥物誘導之牙齦過度生長,凝血酶,α型平滑肌肌動蛋白,EGCG,lovastatin,薑黃素, | zh_TW |
| dc.subject.keyword | drug-induced gingival overgrowth (DIGO),thrombin,α-SMA,EGCG,lovastatin,curcumin, | en |
| dc.relation.page | 43 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-08-18 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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