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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張富雄 | |
dc.contributor.author | Hsiang-Yuan Yao | en |
dc.contributor.author | 姚翔元 | zh_TW |
dc.date.accessioned | 2021-06-07T23:57:04Z | - |
dc.date.copyright | 2013-09-24 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-19 | |
dc.identifier.citation | Han G, Ghosh P, Rotello VM (2007) Multi-functional gold nanoparticles for drug delivery. Adv Exp Med Biol 620: 48-56
Jennings T, Strouse G (2007) Past, present, and future of gold nanoparticles. Adv Exp Med Biol 620: 34-47 Hoshino A, Manabe N, Fujioka K, Suzuki K, Yasuhara M, Yamamoto K (2007) Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity. J Artif Organs 10: 149-157 Kaji N, Tokeshi M, Baba Y (2007) Single-molecule measurements with a single quantum dot. Chem Rec 7: 295-304 Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4: 435-446 Smith AM, Gao X, Nie S (2004) Quantum dot nanocrystals for in vivo molecular and cellular imaging. Photochem Photobiol 80: 377-385 Hsu, M.H., and Su, Y.C. (2008). Iron-oxide embedded solid lipid nanoparticles for magnetically controlled heating and drug delivery. Biomedical microdevices 10, 785-793 Bajpai V, Dai L, Ohashi T (2004) Large-scale synthesis of perpendicularly aligned helical carbon nanotubes. J Am Chem Soc 126: 5070-5071 Li WZ, Xie SS, Qian LX, Chang BH, Zou BS, Zhou WY, Zhao RA, Wang G (1996) Large-Scale Synthesis of Aligned Carbon Nanotubes. Science 274: 1701-1703 Reinlib L, Field L (2000) Cell transplantation as future therapy for cardiovascular disease?: A workshop of the National Heart, Lung, and Blood Institute. Circulation 101: E182-187 Shah K (2005) Current advances in molecular imaging of gene and cell therapy for cancer. Cancer Biol Ther 4: 518-523 Petri-Fink A, Steitz B, Finka A, Salaklang J, Hofmann H (2008) Effect of cell media on polymer coated superparamagnetic iron oxide nanoparticles (SPIONs): colloidal stability, cytotoxicity, and cellular uptake studies. Eur J Pharm Biopharm 68: 129-137 Arbab AS, Yocum GT, Kalish H, Jordan EK, Anderson SA, Khakoo AY, Read EJ, Frank JA (2004) Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood 104: 1217-1223 Lewin M, Carlesso N, Tung CH, Tang XW, Cory D, Scadden DT, Weissleder R (2000) Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells. Nat Biotechnol 18: 410-414 Bulte JW, Kraitchman DL (2004) Iron oxide MR contrast agents for molecular and cellular imaging. NMR Biomed 17: 484-499 Weissleder R, Lee AS, Fischman AJ, Reimer P, Shen T, Wilkinson R, Callahan RJ, Brady TJ (1991) Polyclonal human immunoglobulin G labeled with polymeric iron oxide: antibody MR imaging. Radiology 181: 245-249 Douziech-Eyrolles L, Marchais H, Herve K, Munnier E, Souce M, Linassier C, Dubois P, Chourpa I (2007) Nanovectors for anticancer agents based on superparamagnetic iron oxide nanoparticles. Int J Nanomedicine 2: 541-550 Hanessian S, Grzyb JA, Cengelli F, Juillerat-Jeanneret L (2008) Synthesis of chemically functionalized superparamagnetic nanoparticles as delivery vectors for chemotherapeutic drugs. Bioorg Med Chem 16: 2921-2931 Hong G, Yuan R, Liang B, Shen J, Yang X, Shuai X (2008) Folate-functionalized polymeric micelle as hepatic carcinoma-targeted, MRI-ultrasensitive delivery system of antitumor drugs. Biomed Microdevices 5, 693-700 Davis, M.E., Chen, Z.G., and Shin, D.M. (2008). Nanoparticle therapeutics: an emerging treatment modality for cancer. Nature reviews Drug discovery 7, 771-782 Sugahara, K.N., Teesalu, T., Karmali, P.P., Kotamraju, V.R., Agemy, L., Greenwald, D.R., and Ruoslahti, E. (2010). Coadministration of a tumor-penetrating peptide enhances the efficacy of cancer drugs. Science 328, 1031-1035. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17115 | - |
dc.description.abstract | 奈米科技的研究廣泛在各個領域上,而在生醫領域上,主要有組織再生材料、影像顯影追蹤的顯影劑還有治療癌症疾病的載體。而在治療癌症中,奈米粒子被運用的方式有很多,有物理化療的方式也有將奈米粒子做為藥物的載體。而在做藥物載體的研究時,最大的挑戰就是如何將此載體安全運送到目的地,必須不被身體內的免疫細胞給清除,也不能大部分堆積在肝臟或腎臟,而有效率地送到癌症發生的地方,並且奈米粒子只能專一傳遞給癌細胞,而不能非專一性的遞送,以免造成其他細胞死亡。我們所使用的氧化鐵奈米粒子具有磁性,並且用微脂體包覆藥物在其中,當包覆藥物的奈米粒子在遞送上,可藉由外在磁場引導氧化鐵奈米粒子到作用位置。實驗室所建構的PAST蛋白,是由protein A跟strepavidin所組成的融合蛋白,我們使用中性脂微粒表面修飾biotin,再包覆氧化鐵奈米粒子,製備成磁性奈米脂微粒,biotin可藉由strepavidin與PAST結合,而protein A 可與特定抗體結合,利用此混和好的複合物,即可標定到有特定受體的細胞,就可提高專一性毒殺癌細胞的效果。在本研究中將分析:1. 奈米脂微粒與氧化鐵複合奈米粒子之粒徑分析,; 2. 導向磁性奈米脂微粒專一性標定細胞,利用螢光訊號驗證經由修飾過的複合奈米粒子,是否對特定的細胞具有專一性的遞送; 3. 用包覆藥物的奈米粒子證明,是否有複合式的奈米粒子,可以針對特定的細胞產生細胞毒殺作用。 | zh_TW |
dc.description.abstract | Liposomes are lipid-based particles and have been reported in clinical applications. Used as drug delivery systems in cancer treatment, a series of strategies have been developed and modified to enhance the therapeutic efficacy of liposomal drugs. The straightforward strategy is designing tumor-specific targeting of liposomal drugs. Therefore we used modified liposomes by specific interaction between ligands and tumor cells for targeting. We constructed an adaptor protein between liposomes and antibodies for cancer cell targeting. Protein A-streptavidin (PAST), a bifunctional fusion protein, could link nanostures of biotinylated liposomes and antibodies together. The cationic liposomes have been used in nanoparticle research recently. We first examined cationic lipid based combination of liposomes for cell targeting. But the big problem is non-specific binding. Since targeted delivery is the major concern in cancer therapeutic studies. Therefore, we changed to another combination of liposomes, using neutral lipids, which can prevent from non-specific binding. The specific targeting strategy can be achieved by linking cellular ligands to the surface of nanoparticles. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T23:57:04Z (GMT). No. of bitstreams: 1 ntu-102-R00442033-1.pdf: 617012 bytes, checksum: 40c2ba796a09106777495343f7d82303 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 ii 中文摘要 iii ABSTRACT iv 目錄 v 第一章 緒論 1 1.1 奈米科技基本介紹………………………………………………………………1 1.2 氧化鐵奈米粒子…………………………………………………………………1 1.2.1 氧化鐵奈米粒子在細胞標記和藥物遞送之應用…………………………...1 1.2.2 修飾氧化鐵奈米粒子的包覆材料…………………………………………...2 1.3 PAST連接性融合蛋白…………………………………………………………...2 1.3.1 以PAST蛋白和抗體提高奈米粒子進入細胞的專一性…………………….2 1.4 研究動機…………………………………………………………………………3 第二章 實驗材料與方法 4 2.1 實驗材料 4 2.1.1 細胞株 4 2.1.2 奈米粒子……………………………….…………………………………..4 2.1.3 脂質…………………………………….…………………………………..4 2.1.4 藥品………………………………………………………………………...4 2.1.5 抗體………………………………………………………………………...5 2.2 實驗方法…………..……………………………………………………………..5 2.2.1 氧化鐵奈米粒子(Fe3O4)合成步驟………………………………………...5 2.2.2 磁性奈米脂微粒之製備…………………………………………………...6 2.2.3 純化PAST蛋白質………………………………………………………....7 2.2.4 PAST蛋白質定量與定性………………………………………………….8 2.2.5 製備導向磁性奈米脂微粒與蛋白質的複合體…………………………...8 2.2.6 以雷射共軛焦顯微鏡觀察脂微粒在細胞中的分佈……………………...9 2.2.7 使用高效液相色譜法(HPCL) 偵測在磁性奈米脂微粒的藥物含量……..9 第三章 實驗結果 10 3.1 脂微粒包覆氧化鐵奈米粒子的製備與分析……………………………………10 3.2 PAST蛋白製備與活性測定……………………………………………………..10 3.3 磁性奈米脂微粒接合胜肽的製備和對於細胞吸收與分佈的影響……………11 3.4 利用高效液相色譜法做出紫杉醇的相對定量,偵測不同時間內磁性奈米脂微粒還殘留的紫杉醇含量………………………………………………………….…12 3.5 利用不同組合的磁性奈米脂微粒,並且包覆藥物佐證是否對特定細胞有 專一性的毒殺效果……………………………………………………………….12 第四章 討論 13 4.1 脂微粒包覆氧化鐵奈米粒子的製備與分析……………………………………..13 4.2磁性奈米脂微粒接合胜肽的製備和對於細胞吸收與分佈的影響………….…..13 4.3 利用不同組合的磁性奈米脂微粒,並且包覆藥物佐證是否對特定細胞有 專一性的毒殺效果……………………………………………………………….13 第五章 圖表與說明 14 表一、奈米脂微粒與氧化鐵複合奈米粒子之粒徑分析…………………………..…14 圖一、導向磁性奈米脂微粒專一性標定AU565細胞………………………………14 圖二、導向磁性奈米脂微粒專一性標定AU565細胞的雷射共軛焦顯微鏡影像…15 圖三、流式細胞儀偵測細胞內導向磁性奈米脂微粒的螢光量……………………..16 圖四、 利用高效液相色譜法做出紫杉醇的相對定量,此為標準曲線……………17 圖五、 利用高效液相色譜法做出紫杉醇的相對定量,偵測不同時間內磁性奈米 脂微粒還殘留的紫杉醇含量………………………………………………...17 第六章 參考文獻 18 | |
dc.language.iso | zh-TW | |
dc.title | 利用連接蛋白質快速組合成標靶奈米粒子以利體外藥物遞送之評估研究 | zh_TW |
dc.title | Quick formation of targeted nanoparticles for in vitro
evaluation of drug delivery using adaptor protein as the linker | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張明富,林文澧,梁碧惠 | |
dc.subject.keyword | 奈米粒子,連接蛋白質, | zh_TW |
dc.subject.keyword | liposome,taxol, | en |
dc.relation.page | 20 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2013-08-19 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 生物化學暨分子生物學研究所 | zh_TW |
顯示於系所單位: | 生物化學暨分子生物學科研究所 |
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