請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17093
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭秋萍(Chiu-Ping Cheng) | |
dc.contributor.author | Sim Chong | en |
dc.contributor.author | 張芯 | zh_TW |
dc.date.accessioned | 2021-06-07T23:56:14Z | - |
dc.date.copyright | 2013-08-25 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-08-20 | |
dc.identifier.citation | 楊宗霖。2011。ERF B1-a 基因群在植物青枯病與非生物逆境反應之功能研究。國立台灣大學植物科學研究所碩士論文。
Aguilar, I., Alamillo, J.M., Garcia-Olmedo, F., and Rodriguez-Palenzuela, P. (2002). Natural variability in the Arabidopsis response to infection with Erwinia carotovora subsp. carotovora. Planta 215, 205-209. Asselbergh, B., Curvers, K., Franca, S.C., Audenaert, K., Vuylsteke, M., Van Breusegem, F., and Hofte, M. (2007). Resistance to Botrytis cinerea in sitiens, an abscisic acid-deficient tomato mutant, involves timely production of hydrogen peroxide and cell wall modifications in the epidermis. Plant Physiol 144, 1863-1877. Atkinson, N.J., and Urwin, P.E. (2012). The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63, 3523-3543. Ben, C., Debelle, F., Berges, H., Bellec, A., Jardinaud, M.F., Anson, P., Huguet, T., Gentzbittel, L., and Vailleau, F. (2013). MtQRRS1, an R-locus required for Medicago truncatula quantitative resistance to Ralstonia solanacearum. New Phytol 199, 758-772. Chen, Y.Y., Lin, Y.M., Chao, T.C., Wang, J.F., Liu, A.C., Ho, F.I., and Cheng, C.P. (2009). Virus-induced gene silencing reveals the involvement of ethylene-, salicylic acid- and mitogen-activated protein kinase-related defense pathways in the resistance of tomato to bacterial wilt. Physiol Plant 136, 324-335. Chinchilla, D., Zipfel, C., Robatzek, S., Kemmerling, B., Nurnberger, T., Jones, J.D., Felix, G., and Boller, T. (2007). A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature 448, 497-500. Dang, F.F., Wang, Y.N., Yu, L., Eulgem, T., Lai, Y., Liu, Z.Q., Wang, X., Qiu, A.L., Zhang, T.X., Lin, J., Chen, Y.S., Guan, D.Y., Cai, H.Y., Mou, S.L., and He, S.L. (2013). CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection. Plant Cell Environ 36, 757-774. Denance, N., Ranocha, P., Oria, N., Barlet, X., Riviere, M.P., Yadeta, K.A., Hoffmann, L., Perreau, F., Clement, G., Maia-Grondard, A., van den Berg, G.C., Savelli, B., Fournier, S., Aubert, Y., Pelletier, S., Thomma, B.P., Molina, A., Jouanin, L., Marco, Y., and Goffner, D. (2012). Arabidopsis wat1 (walls are thin1)-mediated resistance to the bacterial vascular pathogen, Ralstonia solanacearum, is accompanied by cross-regulation of salicylic acid and tryptophan metabolism. Plant J 73, 225-239. Dodds, P.N., and Rathjen, J.P. (2010). Plant immunity: towards an integrated view of plant-pathogen interactions. Nat Rev Genet 11, 539-548. Dong, C.J., and Liu, J.Y. (2010). The Arabidopsis EAR-motif-containing protein RAP2.1 functions as an active transcriptional repressor to keep stress responses under tight control. BMC Plant Biol 10, 47. Feng, D.X., Tasset, C., Hanemian, M., Barlet, X., Hu, J., Tremousaygue, D., Deslandes, L., and Marco, Y. (2012). Biological control of bacterial wilt in Arabidopsis thaliana involves abscissic acid signalling. New Phytol 194, 1035-1045. Fujiwara, A., Fujisawa, M., Hamasaki, R., Kawasaki, T., Fujie, M., and Yamada, T. (2011). Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages. Appl Environ Microbiol 77, 4155-4162. Glazebrook, J. (2005). Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol. 43, 205-227. Geraats, P.B.J., Bakker, P.A.H.M., Lawrence, C.B., Achuo E.A., Hofte, M., and van Loon, L.C. (2003). Ethylene-insensitive tobacco shows differentially altered susceptibility to different pathogens. Phytopathology 93:7, 813-821 Gutterson, N., and Reuber, T.L. (2004). Regulation of disease resistance pathways by AP2/ERF transcription factors. Curr Opin Plant Biol 7, 465-471. Hernandez-Blanco, C., Feng, D.X., Hu, J., Sanchez-Vallet, A., Deslandes, L., Llorente, F., Berrocal-Lobo, M., Keller, H., Barlet, X., Sanchez-Rodriguez, C., Anderson, L.K., Somerville, S., Marco, Y., and Molina, A. (2007). Impairment of cellulose synthases required for Arabidopsis secondary cell wall formation enhances disease resistance. Plant cell 19, 890-903. Hiruma, K., Fukunaga, S., Bednarek, P., Pislewska-Bednarek, M., Watanabe, S., Narusaka, Y., Shirasu, K., and Takano, Y. (2013). Glutathione and tryptophan metabolism are required for Arabidopsis immunity during the hypersensitive response to hemibiotrophs. Proc Natl Acad Sci U S A 110, 9589-9594. Ishihara, T., Mitsuhara, I., Takahashi, H., and Nakaho, K. (2012). Transcriptome analysis of quantitative resistance-specific response upon Ralstonia solanacearum infection in tomato. PLoS One 7, e46763. Jacobs, J.M., Babujee, L., Meng, F., Milling, A., and Allen, C. (2012). The in planta transcriptome of Ralstonia solanacearum: conserved physiological and virulence strategies during bacterial wilt of tomato. mBio 3, e00114. Katiyar-Agarwal, S., Morgan, R., Dahlbeck, D., Borsani, O., Villegas, A., Jr., Zhu, J.K., Staskawicz, B.J., and Jin, H. (2006). A pathogen-inducible endogenous siRNA in plant immunity. Proc Natl Acad Sci U S A 103, 18002-18007. Kim, Y.S., Choi, D., Lee, M.M., Lee, S.H., and Kim, W.T. (1998). Biotic and abiotic stress-related expression of 1-aminocyclopropane-1-carboxylate oxidase gene family in Nicotiana glutinosa L. Plant Cell Physiol 39, 565-673. Lai, Y., Dang, F., Lin, J., Yu, L., Shi, Y., Xiao, Y., Huang, M., Lin, J., Chen, C., Qi, A., Liu, Z., Guan, D., Mou, S., Qiu, A., and He, S. (2013). Overexpression of a Chinese cabbage BrERF11 transcription factor enhances disease resistance to Ralstonia solanacearum in tobacco. Plant Physiol Biochem 62, 70-78. Lebeau, A., Gouy, M., Daunay, M.C., Wicker, E., Chiroleu, F., Prior, P., Frary, A., Dintinger, J. (2012). Genetic mapping of a major dominant gene for resistance to Ralstonia solanacearum in eggplant. Theor Appl Genet. 120, 143-158. Li, Z., Zhang, L., Yu, Y., Quan, R., Zhang, Z., Zhang, H., and Huang, R. (2011). The ethylene response factor AtERF11 that is transcriptionally modulated by the bZIP transcription factor HY5 is a crucial repressor for ethylene biosynthesis in Arabidopsis. Plant J 68, 88-99. Liu, J.H., Lee-Tamon, S.H., and Reid, D.M. (1997). Differential and wound-inducible expression of 1-aminocyclopropane-1-carboxylate oxidase genes in sunflower gene. Plant Mol Biol 34, 923-933. Lu, J., Ju, H., Zhou, G., Zhu, C., Erb, M., Wang, X., Wang, P., and Lou, Y. (2011). An EAR-motif-containing ERF transcription factor affects herbivore-induced signaling, defense and resistance in rice. Plant J 68, 583-596. McGrath, K.C., Dombrecht, B., Manners, J.M., Schenk, P.M., Edgar, C.I., Maclean, D.J., Scheible, W.R., Udvardi, M.K., and Kazan, K. (2005). Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression. Plant physiol 139, 949-959. Melotto, M., Underwood, W., Koczan, J., Nomura, K., and He, S.Y. (2006). Plant stomata function in innate immunity against bacterial invasion. Cell 126, 969-980. Mizoi, J., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2012). AP2/ERF family transcription factors in plant abiotic stress responses. Biochim Biophys Acta 1819, 86-96. Ohme-Takagi, M., and Shinshi, H. (1995). Ethylene-inducible DNA binding proteins that interact with ethylene responsive element. Plant Cell 7, 173-182. Ohta, M., Matsui, K., Hiratsu, K., Shinshi, H., and Ohme-Takagi, M. (2001). Repression domains of Class II ERF transcriptional repressors share an essential motif for active repression. Plant Cell 13, 1959-1968. Pan, I.C., Li, C.W., Su, R.C., Cheng, C.P., Lin, C.S., and Chan, M.T. (2010). Ectopic expression of an EAR motif deletion mutant of SlERF3 enhances tolerance to salt stress and Ralstonia solanacearum in tomato. Planta 232, 1075-1086. Palva, T.K., Hurtig, M., Saindrenan, and Palva, E.T. (1994). Salicylic acid induced resistance to Erwinia carotovora subsp. carotovora in Tobacco. Mol Plant Microbe Interact 7, 356-363. Pieterse, C.M., Van der Does, D., Zamioudis, C., Leon-Reyes, A., and Van Wees, S.C. (2012). Hormonal modulation of plant immunity. Annu Rev Cell Dev Biol 28, 489-521. Pirrello, J., Prasad, B.C. N., Zhang, W.S., Chen, K.S., Mila, I., Zouine., M., Latche, A., Pech, J.C., Ohme-Takagi, M., Regad. R. and Bouzayen. M. (2012). Functional analysis and binding affinity of tomato ethylene response factors provide insight on the molecular bases of plant differential responses to ethylene. BMC Plant Biol 12, 190. Porra, R.J., Schafer, W., Cmiel, E., Katheder, I. and Scheer, H. (1994). The derivation of the formyl-group oxygen of chlorophyll b in higher plants from molecular oxygen Achievement of high enrichment of the7-formyl-group oxygen from 18O2 in greening maize leaves. Eur J Biochem. 219, 671 -679. Rizhsky, L., Davletova, S., Liang, H., and Mittler, R. (2004). The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J Biol Chem. 279, 11736-11743. Rizhsky, L., Liang, H., Shuman, J., Shulaev, V., Davletova, S., and Mittler, R. (2004). When defense pathways collide. The response of Arabidopsis to a combination of drought and heat stress. Plant physiol 134, 1683-1696. Sakuma, Y., Liu, Q., Dubouzet, J.G., Abe, H., Shinozaki, K., and Yamaguchi-Shinozaki, K. (2002). DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun. 290, 998-1009. Sharma, M.K., Kumar, R., Solanke, A.U., Sharma, R., Tyagi, A.K., and Sharma, A.K. (2010). Identification, phylogeny, and transcript profiling of ERF family genes during development and abiotic stress treatments in tomato. Mol Genet Genomics. 284, 455-475. Song, C.P., Agarwal, M., Ohta, M., Guo, Y., Halfter, U., Wang, P., and Zhu, J.K. (2005). Role of an Arabidopsis AP2/EREBP-type transcriptional repressor in abscisic acid and drought stress responses. Plant cell 17, 2384-2396. Torres, M.A. (2010). ROS in biotic interactions. Physiol Plant. 138, 414-429. Tournier, B., Sanchez-Ballesta, M.T., Jones, B., Pesquet, E., Regad, F., Latche, A., Pech, J.-C., and Bouzayen, M. (2003). New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element. FEBS Lett. 550, 149-154. Upadhyay, R.K., Soni, D.K., Singh, R., Dwivedi, U.N., Pathre, U.V., Nath, P., and Sane, A.P. (2013). SlERF36, an EAR-motif-containing ERF gene from tomato, alters stomatal density and modulates photosynthesis and growth. J Exp Bot 64, 3237–3247. Van der Linden, L., Bredenkamp, J., Naidoo, S., Fouche-Weich, J., Denby, K.J., Genin, S., Marco, Y., and Berger, D.K. (2013). Gene-for-gene tolerance to bacterial wilt in Arabidopsis. Mol Plant Microbe Interact 26, 398-406. Wang, J.F., Oliver, J., Thoquet, P., Mangin, B., Sauviac, L., and Grimsley, N.H. (2000). Resistance of tomato line Hawaii7996 to Ralstonia solanacearum Pss4 in Taiwan ss controlled mainly by a major strain-specific locus. Mol Plant Microbe Interact 13, 6-13. Wang, J.-F., Ho, F.-I., Truong, H.T.H., Huang, S.-M., Balatero, C.H., Dittapongpitch, V., and Hidayati, N. (2012). Identification of major QTLs associated with stable resistance of tomato cultivar ‘Hawaii 7996’ to Ralstonia solanacearum. Euphytica 190, 241-252. Yan, Y., Zhang, Y., Yang, K., Sun, Z., Fu, Y., Chen, X., and Fang, R. (2011). Small RNAs from MITE-derived stem-loop precursors regulate abscisic acid signaling and abiotic stress responses in rice. Plant J 65, 820-828. Yang, Z., Tian, L., Latoszek-Green, M., Brown, D., and Wu, K. (2005). Arabidopsis ERF4 is a transcriptional repressor capable of modulating ethylene and abscisic acid responses. Plant Mol Biol 58, 585-596. Zhou, J., Zhang, H., Yang, Y., Zhang, Z., Zhang, H., Hu, X., Chen, J., Wang, X.C., and Huang, R. (2008). Abscisic acid regulates TSRF1-mediated resistance to Ralstonia solanacearum by modifying the expression of GCC box-containing genes in tobacco. J Exp Bot 59, 645-652. Zhu, Z., Shi, J., Cao, J., He, M., and Wang, Y. (2012). VpWRKY3, a biotic and abiotic stress-related transcription factor from the Chinese wild Vitis pseudoreticulata. Plant Cell Rep. 31, 2109-2120. Zipfel, C. (2008). Pattern-recognition receptors in plant innate immunity. Curr Opin Immunol 20, 10-16. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17093 | - |
dc.description.abstract | 植物在自然環境中常遭受各種逆境脅迫,造成植物生長與農作物產量影響極巨,例如,由青枯病菌(Ralstonia solanacearum, Rs)引起的青枯病(bacterial wilt, BW)與缺水逆境都會導致植物急速死亡,造成農業上重大損失。植物為了生存演化出一套複雜的調控機制以調控並抵禦逆境反應,而乙烯轉錄因子(ethylene-response factor, ERFs)是植物特有的轉錄因子且參與許多逆境反應。我們先前的研究顯示在番茄中的兩個屬於VIII群ERF的成員SlERF36與SlERF39可能在青枯病與乾旱防禦反應具一定的重要性,故本研究旨在利用過量表現與基因靜默的轉殖植物研究策略進一步分析這兩個基因在植物逆境反應之確切功能與其調控機制。由結果發現SlERF36蛋白確實具有抑制轉錄活性的功能;過量表現SlERF36可能會使轉殖菸草對青枯病感病些微增加、對滲透壓逆境及乾旱抗性降低,推測SlERF36可能抑制抗病與抗旱相關啟動基因(activators)表現而造成這些性狀。另外,研究結果顯示SlERF39具有抑制轉錄活性的功能;過量表現SlERF39對青枯病抗性降低、對滲透壓及乾旱逆境反應降低,而較抗鹽害逆境,推論SlERF39可能使ET與JA訊息傳遞之抑制基因(repressors)表現降低進而增加ET與JA表現。SlERF39可能抑制促進基因表現進而對缺水逆境抗性降低,而藉由抑制啟動基因表現對鹽害逆境抗性增加。未來可利用本研究所得之過量表現與基因靜默之轉殖植物,更深入探討SlERF36與SlERF39在各種逆境反應之功能,希望有助於釐清對植物病害與非生物逆境之複雜的交互作用,並增加對這些逆境防禦機制的了解並加以運用。 | zh_TW |
dc.description.abstract | Plant constantly encounters environmental stresses such as drought, salt, heat even pathogens and pest, which effect plant growth and crop productivity. Bacterial wilt (BW), which is caused by Ralstonia solanacearum, is a very complex deadly disease and shares certain common features with water stress responses induced plant death and crop loss. Hence, the plants have evoluted a complex mechanism to regulate and defense to stress responses. Ethylene response factors (ERFs) is a large family of plant-specific transcription factors involved in various stress responses. Our previous study revealed that two Group VIII ERFs from tomato, namely SlERF36 and SlERF39 may involve in BW and drought responses. In this study, transgenic plant with altered expression levels of these genes were generated and analyzed for their stress responses and the involvement regulatory mechanism. The result show that SlERF36 was a functional transcriptional repressor. Overexpression of SlERF36 in transgenic tobacco plants demonstrated that SlERF36 increased susceptibility to BW, decreased osmotic and water tolerance. It is hypothesized that SlERF36 may reduce the expression of defense response and water tolerance response by suppressing the expression of defense response and drought tolerant activators. SlERF35 also function as a transcriptional repressor. Overexpression of SlERF39 cause increased susceptibility to BW and decreased in water stress tolerance, increased expression of ethylene (ET) and jasmonic acid (JA) biosynthesis genes. It is hypothesized that SlERF39 re-depressed the ET and JA signaling by suppressing the expression of certain repressors. Reduced expression of SA led to susceptibility to BW. SlERF39 may reduce the expression of water tolerance response by suppressing the expression of drought tolerant activators but increased the expression of salt tolerant through suppression of salt tolerant suppressor. The transgenic plants generated in this study can be used for future analyses to gain further insight into disease resistant and abiotic cross-talk mechanism of these proteins. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T23:56:14Z (GMT). No. of bitstreams: 1 ntu-102-R99B42032-1.pdf: 2418463 bytes, checksum: 962f7a4d18df506aedd626fece6471d4 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iii ABSTRACT iv 縮寫與全名對照表 v 目錄 vii 圖目錄 ix 附錄目錄 x 第一章 前言 1 植物病害防禦機制 1 植物病害與非生物逆境之交互作用 1 青枯病 (Bacterial wilt, BW) 之介紹 2 青枯病反應相關研究 2 AP2 (APETALA2)/ERF 植物乙烯轉錄因子超級家族 3 研究動機 4 第二章 材料與方法 6 1. 植物材料簡介 6 2. 基因選殖常用實驗 6 3. 轉錄活性分析法Transactivation assay 9 4. 番茄以真空吸引浸透法 (Vacuum-infiltration) 接種青枯病菌、細菌性斑點病、細菌性黑腐病及軟腐病之樣品製備 10 5. 檢測基因表現 11 6. 植物轉殖 12 7. 植物之逆境檢測 14 第三章 結果 18 1. 利用生物資訊分析番茄SlERF36與SlERF39基因結構組成及其序列比對分析 18 2. SlERF36與SlERF39 調控轉錄活性分析 (Transactivation assay) 18 3. 35S::SlERF36轉殖菸草之性狀分析 19 4. SlERF36-RNAi番茄轉殖株性狀分析 20 5. 35S::SlERF39轉殖菸草之性狀分析 21 6. SlERF39-RNAi番茄轉殖株性狀分析 23 第四章 討論 25 1. SlERF36與SlERF39為抑制子型 (repressor-type) 轉錄因子 25 2. SlRF36與SlERF39可能負調控植物抗青枯病反應,但可能未參與細菌性軟腐病害反應 25 3. SlRF36與SlERF39負調控植物乾旱抗性,也參與ABA與滲透壓逆境,但可能未參與鹽害反應 27 4. pCAMBIA1301之空載體番茄轉殖株發病異常 28 5. 總結 28 參考文獻 30 | |
dc.language.iso | zh-TW | |
dc.title | 番茄ERF36與ERF39在逆境反應之功能研究 | zh_TW |
dc.title | Functional study of tomato ERF36 and ERF39 in stress responses | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 詹明才(Ming-Tsair Chan),葉開溫(Kai-Wun Yeh),鄭石通(Shih-Tong Jeng) | |
dc.subject.keyword | 乙烯反應轉錄因子,青枯病,乾旱逆境,鹽害逆境, | zh_TW |
dc.subject.keyword | Ethylene-response factors (ERFs),bacteria wilt (BW),drought,salt stress, | en |
dc.relation.page | 76 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2013-08-20 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 2.36 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。