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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳昭瑩(Chao-Ying Chen) | |
| dc.contributor.author | Yu-Ting Chen | en |
| dc.contributor.author | 陳鈺婷 | zh_TW |
| dc.date.accessioned | 2021-06-07T23:55:53Z | - |
| dc.date.copyright | 2013-08-25 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-20 | |
| dc.identifier.citation | 1. 劉增城。1998。草莓王國大湖鄉。苗栗區農業專訊4 (12)。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17083 | - |
| dc.description.abstract | 本研究探討分離自草莓植株之類芽孢桿菌菌株TP3的生物防治活性及施用方法。菌株TP3與灰黴病菌共培養於馬鈴薯葡萄糖瓊脂培養基上，可形成明顯的抑制圈，菌株TP3具有溶磷的能力，施用於草莓幼苗根圈不影響植株的生長。進行溫室及田間試驗，發現施用菌株TP3營養細胞或內孢子懸浮液於草莓植株地上部表面，對草莓灰黴病具有良好的防治效果，保護率達67.7 ~ 79.3%；此外，澆灌菌株TP3營養細胞或內孢子懸浮液於草莓植株根圈，亦可抑制草莓葉灰黴病的發病程度。含有幾丁聚醣之馬鈴薯葡萄糖培養基並不影響草莓灰黴病菌菌絲的生長，但若將濃度5×107 CFU/ml之菌株TP3營養細胞懸浮液與幾丁聚醣共同施用於草莓植株表面，可抑制灰黴病的發病程度，且在田間試驗施用菌株TP3 (2×107 CFU/ml)混合幾丁聚醣可促進草莓果實的產量。除了草莓灰黴病之外，菌株TP3營養細胞懸浮液施用於玫瑰花及百合葉上，亦可抑制灰黴病的病徵發展。菌株TP3液態培養之上清液對灰黴病菌孢子發芽具有顯著的抑制效果，進一步利用DEAE FF離子交換管柱進行層析純化及LC-MS/MS分析，自馬鈴薯葡萄糖培養液中獲得部分純化之菌株TP3外泌抗菌物質，推測其為新穎蛋白質。 | zh_TW |
| dc.description.abstract | In this research, Paenibacillus sp. TP3 screened from strawberry plants showed antagonistic activity against Btrytis cinerea in dual culture assay. Paenibacillus sp. TP3 showed phosphate-solubilizing activity and did not have negative effect on the growth of strawberry plantlet by soil drench. Pot assays and field trials by foliar spray with vegetative cell suspension of Paenibacillus sp. TP3 showed 67.7 ~ 79.3% inhibition of gray mold disease in strawberry. Furthermore, soil drench of TP3 vegetative cell or endospore suspension also suppressed gray mold disease in strawberry. Although hyphal growth of B. cinerea was not inhibited on chitosan-containing PDA medium, treatment with Paenibacillus sp. TP3 suspension of 5×107 CFU/ml combining with chitosan on strawberry plantlets suppressed gray mold development. Moreover, treatment with Paenibacillus sp. TP3 of 2×107 CFU/ml in combination with chitosan improved the fruit production of strawberry in field trials. Besides, treatment with vegetative cell suspension on rose flower and lily leaves suppressed gray mold symptoms. Since bacteria-free culture supernatant showed significant suppression on spore germination, the anti-Botrytis acting compounds were further purified by DEAE ion exchange chromatography, and performed LC-MS/MS analysis. According to the result, the anti-Botrytis substances produced by Paenibacillus sp. TP3 were presumed to be novel proteins. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T23:55:53Z (GMT). No. of bitstreams: 1 ntu-102-R00633014-1.pdf: 1738161 bytes, checksum: ca82ca8f1dcc44e69460aec51053379a (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 壹、摘要 1
貳、前言 3 參、前人研究 4 一、臺灣草莓產業沿革 4 二、草莓灰黴病現況 5 三、草莓灰黴病之化學及非化學防治 6 四、類芽孢桿菌(Paenibacillus)之特性 8 肆、 材料與方法 10 一、供試菌株之培養與保存 10 二、類芽孢桿菌菌株TP3之特性 11 1. 類芽孢桿菌菌株 TP3與灰黴病菌之對峙培養 11 2. 觀察內孢子形成之染色試驗 11 3. 類芽孢桿菌菌株 TP3產孢能力試驗 11 4. 類芽孢桿菌菌株 TP3溶磷能力試驗 11 5. 菌體懸浮液根部澆灌草莓植株生長試驗 12 三、草莓植株之培養條件 12 四、葉面施用之防治試驗 12 五、比較營養細胞及內孢子懸浮液於田間之防治效力（葉面施用） 13 六、菌體懸浮液根部澆灌草莓植株根部之防治試驗 14 七、類芽孢桿菌菌株TP3與幾丁聚醣共同施用之可能性 14 1. 含幾丁聚醣培養基對灰黴病菌菌絲生長速度之影響 14 2. 溫室試驗 15 3. 田間試驗 15 八、其他作物灰黴病之防治 15 1. 玫瑰切花之灰黴病防治試驗 15 2. 百合離葉之灰黴病防治試驗 16 九、抗菌物質生物活性試驗 16 1. 孢子發芽抑制試驗 16 2. 菌絲生長抑制試驗 16 十、抗菌物質特性分析 17 十一、抗菌物質分離純化 17 1. 硫酸銨蛋白質沉澱 17 2. 離子交換樹脂管柱層析純化 17 3. 抗菌活性測試 18 4. SDS-聚丙烯醯胺膠體分析 18 5. 蛋白質鑑定 19 伍、 結果 20 一、類芽孢桿菌菌株TP3之特性 20 1. 抗菌能力 20 2. 內孢子產生能力 20 3. 溶磷試驗 20 4. 菌體懸浮液根部澆灌草莓植株生長試驗 20 二、葉面施用抗病能力溫室試驗 21 三、營養細胞及內孢子懸浮液於田間之防治效力（葉面施用） 21 四、營養細胞懸浮液澆灌草莓植株根部對葉部灰黴病之防治效果 21 五、類芽孢桿菌菌株TP3與幾丁聚醣共同施用之可能性 22 1. 含幾丁聚醣培養基對灰黴病菌菌絲生長速度之影響 22 2. 田間試驗 22 六、其他作物灰黴病之生物防治試驗 23 1. 玫瑰切花之灰黴病生物防治試驗 23 2. 百合離葉之灰黴病生物防治試驗 23 七、抗菌物質分析 23 1. 抗菌物質特性分析 23 2. 抗菌物質分離純化 24 陸、討論 25 柒、參考文獻 30 捌、圖表集 41 表一、菌株TP3在不同培養基、培養天數之產孢情形 42 圖一、對峙培養形成之抑制圈 43 圖二、菌株TP3內孢子 44 圖三、PKA培養基溶磷能力測試 45 圖四、連續8周澆灌菌株TP3後草莓植株葉寬及葉數 46 圖五、草莓植株上之抗灰黴病菌試驗 48 圖六、田間試驗：植株地上部施用菌株TP3營養細胞及內孢子懸浮液之草莓花朵發病嚴重度 49 圖七、田間試驗: 菌株TP3對草莓果實灰黴病之防治試驗 51 圖八、澆灌菌液於草莓根圈對葉部灰黴病之誘導抗病效果 52 圖九、不同幾丁聚醣培養基對灰黴病菌生長之影響 53 圖十、幾丁聚醣施用於草莓植株對灰黴病之防治效果 54 圖十一、苗栗田間施用菌株TP3混合幾丁聚醣對草莓果實產量之影響 55 圖十二、苗栗田間施用菌株TP3混合幾丁聚醣對草莓果實均重之影響 56 圖十三、菌株TP3對玫瑰灰黴病之防治效果 57 圖十四、菌株TP3對百合灰黴病之防治效果 58 圖十五、培養上清液經酵素處理後對灰黴病菌菌絲生長之影響 59 圖十六、蛋白質純化流程 60 圖十七、管柱層析分液之孢子發芽生物活性分析 61 圖十八、部分純化蛋白質之SDS-PAGE條帶 62 | |
| dc.language.iso | zh-TW | |
| dc.title | 草莓灰黴病生物防治之應用研究 | zh_TW |
| dc.title | Applied Research of Biological Control of Strawberry Gray Mold | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳文希(Wen-Shi Wu),黃祥恩(Hsiang-En Huang),鍾嘉綾(Chia-Lin Chung) | |
| dc.subject.keyword | 草莓,灰黴病,類芽孢桿菌,生物防治,幾丁聚醣,抗菌蛋白, | zh_TW |
| dc.subject.keyword | strawberry,Botrytis cinerea,Paenibacillus,biological control,chitosan,antifungal protein, | en |
| dc.relation.page | 62 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-08-20 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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