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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張惠婷 | |
| dc.contributor.author | Chi-Ya Huang | en |
| dc.contributor.author | 黃琪雅 | zh_TW |
| dc.date.accessioned | 2021-06-07T23:53:35Z | - |
| dc.date.copyright | 2013-11-05 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-10-18 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17024 | - |
| dc.description.abstract | 本研究之目的為探討黃連木（Pistacia chinensis）枝條、葉子精油與抽出物抑制黃嘌呤氧化酶之活性，期盼開發黃連木於醫療保健領域的新用途。研究結果顯示黃連木枝條與葉子的乙醇抽出物、丙酮抽出物、熱水抽出物與精油中，以葉子精油抑制黃嘌呤氧化酶的效果最為顯著。 葉子精油的6個分離部中，以E1分離部最能抑制黃嘌呤氧化酶的催化；經由成分分析顯示，葉子精油與分離部E1的主要成分均為Limonene，相對含量分別為23.44%和61.06%，其他次要成分尚有cis-β-Ocimene、Terpinen-4-ol、α-Thujene、α-Pinene、4-Carene與3-Carene等。精油主成分Limonene經定量分析顯示，(+)-Limonene之含量明顯高於(-)-Limonene。成分中具有減少尿酸形成的化合物為Myrcene、(-)-Limonene與(+)-Limonene，使用次黃嘌呤為受質時，化合物的半數抑制濃度分別為36.88、33.53和37.69 μg/mL，而抑制酵素催化黃嘌呤為尿酸之IC50值則介於41.05－48.04 μg/mL。 精油、E1分離部與有效成分進行酵素動力學分析，發現E1分離部、Myrcene與(-)-Limonene是以競爭型方式阻礙酵素催化次黃嘌呤生成尿酸，而黃連木葉子精油、Camphene、(+)-Limonene和γ-Terpinene則為混合型態；當受質為黃嘌呤時，全部試樣皆為混合型的方式影響黃嘌呤氧化酶與黃嘌呤的親和力。綜合上述結果，可知黃連木葉子精油具有潛力發展為治療痛風的天然藥物。 | zh_TW |
| dc.description.abstract | The aim of this study was to evaluate the xanthine oxidase inhibitiory activities of oils and extracts from Pistacia chinensis leaf and twig. According to xanthine oxidase inhibition results, leaf oil exhibited the most excellent performance whether hypoxanthine or xanthine was used as the substrate. Among six fractions of leaf oil, E1 fraction possessed the best xanthine oxidase inhibition activity. Results from the chemical composition analysis of leaf oil and E1 fraction, both specimens showed limonene was the main compound with the relative content of 23.44% and 61.06%, respectively. The other compounds from leaf oil had cis-β-ocimene, terpinen-4-ol, α-thujene, α-pinene, 4-carene and 3-carene. In quantitative analysis of limonene, both leaf oil and E1 fraction had a much higher content of (+)-limonene than that of (-)-limonene. When the substrate was hypoxanthine, myrcene, (-)-limonene and (+)-limonene were the best active compunds against xanthine oxidase with IC50 values of 36.88, 33.53 and 37.69 μg/mL, respectively. When the substrate was xanthine, IC50 values of myrcene, (-)-limonene and (+)-limonene ranged from 41.05 to 48.04 μg/mL. In the enzyme kinetic studies, E1 fraction, myrcene and (-)-limonene exhibited the competitive-type inhibition against xanthine oxidase; and leaf oil, camphene, (+)-limonene and γ-terpinene exhibited the mix-type inhibition when using hypoxanthine as a substrate. When the substrate was xanthine, all active specimens presented the mix-type inhibition. Accordingly, leaf oil from Pistacia chinensis is a natural remedy with a great potential for the treatment of gout. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T23:53:35Z (GMT). No. of bitstreams: 1 ntu-102-R99625047-1.pdf: 2237482 bytes, checksum: 84e9d11fa74c7a6d9b04b69821c5032d (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 摘要 I
Abstract II 目錄 III 表目錄 VII 圖目錄 X 壹、 前言 1 貳、 文獻回顧 3 一、 黃嘌呤氧化酶的性質與作用機制 3 二、 痛風 5 (一) 成因 5 (二) 誘發之因子 7 (三) 常見之症狀 8 (四) 預防與治療方法 8 三、 具有抑制黃嘌呤氧化酶活性之林木天然物 10 (一) 植物抽出物抑制黃嘌呤氧化酶之活性 10 (二) 植物天然化合物抑制黃嘌呤氧化酶之活性 15 四、 黃連木屬植物抽出物及其生物活性探討 24 (一) 黃連木屬植物的特性 24 (二) 黃連木屬植物抽出物之生物活性 25 1. 黃連木屬植物抽出物的抗氧化活性 25 2. 黃連木屬植物抽出物的抗發炎活性 28 3. 黃連木屬植物抽出物的抗菌活性 29 4. 黃連木屬植物抽出物的雌激素活性 30 5. 黃連木屬植物抽出物的鎮痛活性 31 6. 黃連木屬植物抽出物的神經元保護活性 31 7. 黃連木屬植物抽出物的抗遺傳毒性活性 31 參、 材料與方法 33 一、 試驗材料 33 (一) 試材 33 (二) 試驗藥品與溶劑 33 1. 試驗藥品 33 2. 試驗溶劑 33 二、 試驗方法 33 (一) 黃連木抽出物之萃取 33 1. 乙醇抽出物 33 2. 丙酮抽出物 34 3. 熱水抽出物 34 4. 水蒸餾精油 34 (二) 抗氧化活性測定 34 1. 總酚類含量測定 34 2. 總黃酮類含量測定 35 3. 還原力測定 35 4. DPPH自由基清除能力之測定 35 5. 總抗氧化能力測定 36 (三) 抑制黃嘌呤氧化酶活性測定 36 (四) 酵素動力學試驗 37 (五) 精油成分分離與鑑定 38 1. 管柱層析 38 2. 氣相層析 38 (六) 統計分析38 肆、 結果與討論 39 一、 黃連木枝條、葉子精油與抽出物之抗氧化活性 39 (一) 黃連木枝條、葉子精油與抽出物收率 39 (二) 黃連木枝條、葉子精油與抽出物之總酚類含量 40 (三) 黃連木枝條、葉子精油與抽出物之總黃酮類含量 42 (四) 黃連木枝條、葉子精油與抽出物之還原能力 43 (五) 黃連木枝條、葉子精油與抽出物之DPPH自由基捕捉活性 44 (六) 黃連木枝條、葉子精油與抽出物之總抗氧化能力 48 二、 黃連木枝條、葉子精油與抽出物之抑制尿酸生成活性 50 (一) 黃連木枝條、葉子精油與抽出物之黃嘌呤氧化酶抑制效果 50 (二) 黃連木枝條、葉子精油之酵素動力學探討 56 1. 葉子精油之酵素動力學分析 56 2. 枝條精油之酵素動力學分析 58 3. Allopurinol之酵素動力學分析 60 三、 黃連木葉子精油分離部之抑制尿酸生成活性 62 (一) 葉子精油分離部之收率 62 (二) 葉子精油分離部之黃嘌呤氧化酶抑制效果 63 (三) 葉子精油分離部E1之酵素動力學分析 66 四、 黃連木葉子精油之成分分析 68 (一) 葉子精油之成分分析 68 (二) 葉子精油分離部E1之成分分析 71 (三) Limonene之定量分析 73 五、 葉子精油成分之抑制尿酸生成活性 75 (一) 葉子精油成分之黃嘌呤氧化酶抑制效果 75 (二) 葉子精油成分之酵素動力學探討 81 1. Camphene之酵素動力學分析 81 2. Myrcene之酵素動力學分析 83 3. (-)-Limonene之酵素動力學分析 85 4. (+)-Limonene之酵素動力學分析 87 5. γ-Terpinene之酵素動力學分析 89 伍、 結論 91 陸、 參考文獻 92 | |
| dc.language.iso | zh-TW | |
| dc.subject | 檸檬烯 | zh_TW |
| dc.subject | 黃連木 | zh_TW |
| dc.subject | 精油 | zh_TW |
| dc.subject | 黃嘌呤氧化? | zh_TW |
| dc.subject | 抑制黃嘌呤氧化?活性 | zh_TW |
| dc.subject | Essential oil | en |
| dc.subject | Limonene | en |
| dc.subject | Xanthine oxidase inhibitiory activity | en |
| dc.subject | Xanthine oxidase | en |
| dc.subject | Pistacia chinensis | en |
| dc.title | 黃連木葉子精油抑制黃嘌呤氧化酶之活性研究 | zh_TW |
| dc.title | Studies on Xanthine Oxidase Inhibitory Activity of Pistacia chinensis Bunge Leaf oil | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 張上鎮,王升陽,許富蘭 | |
| dc.subject.keyword | 黃連木,精油,黃嘌呤氧化?,抑制黃嘌呤氧化?活性,檸檬烯, | zh_TW |
| dc.subject.keyword | Pistacia chinensis,Essential oil,Xanthine oxidase,Xanthine oxidase inhibitiory activity,Limonene, | en |
| dc.relation.page | 101 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2013-10-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
| 顯示於系所單位： | 森林環境暨資源學系 | |
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