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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 龐飛 | |
dc.contributor.author | Yen-Chen Chang | en |
dc.contributor.author | 張晏禎 | zh_TW |
dc.date.accessioned | 2021-06-07T23:46:47Z | - |
dc.date.copyright | 2014-07-16 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-06-13 | |
dc.identifier.citation | Allan, G. M., F. McNeilly, J. P. Cassidy, G. A. Reilly, B. Adair, W. A. Ellis and M. S. McNulty (1995). Pathogenesis of porcine circovirus; experimental infections of colostrum deprived piglets and examination of pig foetal material. Vet Microbiol 44(1): 49-64.
Balmelli, C., E. Steiner, H. Moulin, N. Peduto, B. Herrmann, A. Summerfield and K. McCullough (2011). Porcine circovirus type 2 DNA influences cytoskeleton rearrangements in plasmacytoid and monocyte-derived dendritic cells. Immunology 132(1): 57-65. Borghetti, P., M. Morganti, R. Saleri, L. Ferrari, E. De Angelis, V. Cavalli, A. Cacchioli, A. Corradi and P. Martelli (2013). Innate pro-inflammatory and adaptive immune cytokines in PBMC of vaccinated and unvaccinated pigs naturally exposed to porcine circovirus type 2 (PCV2) infection vary with the occurrence of the disease and the viral burden. Vet Microbiol 163(1-2): 42-53. Brunborg, I. M., C. M. Moldal T Fau - Jonassen and C. M. Jonassen (2004). Quantitation of porcine circovirus type 2 isolated from serum/plasma and tissue. J Virol Methods 122(2): 171-178. Buer, J., A. Lanoue, A. Franzke, C. Garcia, H. von Boehmer and A. Sarukhan (1998). Interleukin 10 secretion and impaired effector function of major histocompatibility complex class II-restricted T cells anergized in vivo. J Exp Med 187(2): 177-183. Chae, C. (2004). Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet J 168(1): 41-49. Chae, C. (2012). Commercial porcine circovirus type 2 vaccines: efficacy and clinical application. Vet J 194(2): 151-157. Chang, H. W., C. R. Jeng, T. L. Lin, J. J. Liu, M. T. Chiou, Y. C. Tsai, M. Y. Chia, T. R. Jan and V. F. Pang (2006). Immunopathological effects of porcine circovirus type 2 (PCV2) on swine alveolar macrophages by in vitro inoculation. Vet Immunol Immunopathol 110(3-4): 207-219. Chang, H. W., V. F. Pang, L. J. Chen, M. Y. Chia, Y. C. Tsai and C. R. Jeng (2006). Bacterial lipopolysaccharide induces porcine circovirus type 2 replication in swine alveolar macrophages. Vet Microbiol 115(4): 311-319. Chianini, F., N. Majo, J. Segales, J. Dominguez and M. Domingo (2003). Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 94(1-2): 63-75. Choi, C. and C. Chae (1999). In-situ hybridization for the detection of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Comp Pathol 121(3): 265-270. Crisci, E., M. Ballester, J. Dominguez, J. Segales and M. Montoya (2010). Increased numbers of myeloid and lymphoid IL-10 producing cells in spleen of pigs with naturally occurring postweaning multisystemic wasting syndrome. Vet Immunol Immunopathol 136(3-4): 305-310. Darwich, L., M. Balasch, J. Plana-Duran, J. Segales, M. Domingo and E. Mateu (2003). Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. J Gen Virol 84(Pt 12): 3453-3457. Darwich, L. and E. Mateu (2012). Immunology of porcine circovirus type 2 (PCV2). Virus Res 164(1-2): 61-67. Darwich, L., S. Pie, A. Rovira, J. Segales, M. Domingo, I. P. Oswald and E. Mateu (2003). Cytokine mRNA expression profiles in lymphoid tissues of pigs naturally affected by postweaning multisystemic wasting syndrome. J Gen Virol 84(Pt 8): 2117-2125. Darwich, L., J. Segales, M. Domingo and E. Mateu (2002). Changes in CD4(+), CD8(+), CD4(+) CD8(+), and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and healthy pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues. Clin Diagn Lab Immunol 9(2): 236-242. Darwich, L., J. Segales and E. Mateu (2004). Pathogenesis of postweaning multisystemic wasting syndrome caused by Porcine circovirus 2: An immune riddle. Arch Virol 149(5): 857-874. Darwich, L., J. Segales, A. Resendes, M. Balasch, J. Plana-Duran and E. Mateu (2008). Transient correlation between viremia levels and IL-10 expression in pigs subclinically infected with porcine circovirus type 2 (PCV2). Res Vet Sci 84(2): 194-198. de Boisseson, C., V. Beven, L. Bigarre, R. Thiery, N. Rose, E. Eveno, F. Madec and A. Jestin (2004). Molecular characterization of Porcine circovirus type 2 isolates from post-weaning multisystemic wasting syndrome-affected and non-affected pigs. J Gen Virol 85(Pt 2): 293-304. Diaz, I., M. Cortey, L. Darwich, M. Sibila, E. Mateu and J. Segales (2012). Subclinical porcine circovirus type 2 infection does not modulate the immune response to an Aujeszky's disease virus vaccine. Vet J 194(1): 84-88. Dorr, P. M., R. B. Baker, G. W. Almond, S. R. Wayne and W. A. Gebreyes (2007). Epidemiologic assessment of porcine circovirus type 2 coinfection with other pathogens in swine. J Am Vet Med Assoc 230(2): 244-250. Doster, A. R., S. Subramaniam, J. Y. Yhee, B. J. Kwon, C. H. Yu, S. Y. Kwon, F. A. Osorio and J. H. Sur (2010). Distribution and characterization of IL-10-secreting cells in lymphoid tissues of PCV2-infected pigs. J Vet Sci 11(3): 177-183. Dvorak, C. M., S. Puvanendiran and M. P. Murtaugh (2013). Cellular pathogenesis of porcine circovirus type 2 infection. Virus Res 174(1-2): 60-68. Ellis, J., L. Hassard, E. Clark, J. Harding, G. Allan, P. Willson, J. Strokappe, K. Martin, F. McNeilly, B. Meehan, D. Todd and D. Haines (1998). Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Can Vet J 39(1): 44-51. Fenaux, M., T. Opriessnig, P. G. Halbur, F. Elvinger and X. J. Meng (2004). Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo. J Virol 78(24): 13440-13446. Fernandes, L. T., A. Tomas, A. Bensaid, M. Perez-Enciso, M. Sibila, A. Sanchez and J. Segales (2009). Exploratory study on the transcriptional profile of pigs subclinically infected with porcine circovirus type 2. Anim Biotechnol 20(3): 96-109. Fernandes, L. T., A. Tomas, A. Bensaid, M. Sibila, A. Sanchez and J. Segales (2012). Microarray analysis of mediastinal lymph node of pigs naturally affected by postweaning multisystemic wasting syndrome. Virus Res 165(2): 134-142. Fort, M., A. Olvera, M. Sibila, J. Segales and E. Mateu (2007). Detection of neutralizing antibodies in postweaning multisystemic wasting syndrome (PMWS)-affected and non-PMWS-affected pigs. Vet Microbiol 125(3-4): 244-255. Fort, M., M. Sibila, A. Allepuz, E. Mateu, F. Roerink and J. Segales (2008). Porcine circovirus type 2 (PCV2) vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins. Vaccine 26(8): 1063-1071. Fraile, L., M. Sibila M Fau - Nofrarias, R. Nofrarias M Fau - Lopez-Jimenez, E. Lopez-Jimenez R Fau - Huerta, A. Huerta E Fau - Llorens, S. Llorens A Fau - Lopez-Soria, D. Lopez-Soria S Fau - Perez, J. Perez D Fau - Segales and J. Segales (2012). Effect of sow and piglet porcine circovirus type 2 (PCV2) vaccination on piglet. Vet Microbiol 161(1-2): 229-234. Grasland, B., P. Blanchard, A. Keranflec'h, L. Bigault, A. Oger, N. Rose, F. Madec, A. Jestin and R. Cariolet (2013). Evaluation of the transmission of porcine circovirus type 2 (PCV-2) genogroups a and b with semen from infected specific-pathogen-free boars. Vet Microbiol 162(2-4): 381-387. Grau-Roma, L., L. Fraile and J. Segales (2011). Recent advances in the epidemiology, diagnosis and control of diseases caused by porcine circovirus type 2. Vet J 187(1): 23-32. Grau-Roma, L., P. M. Heegaard, C. K. Hjulsager, M. Sibila, C. S. Kristensen, A. Allepuz, M. Pineiro, L. E. Larsen, J. Segales and L. Fraile (2009). Pig-major acute phase protein and haptoglobin serum concentrations correlate with PCV2 viremia and the clinical course of postweaning multisystemic wasting syndrome. Vet Microbiol 138(1-2): 53-61. Grierson, S. S., D. P. King, G. J. Wellenberg and M. Banks (2004). Genome sequence analysis of 10 Dutch porcine circovirus type 2 (PCV-2) isolates from a PMWS case-control study. Res Vet Sci 77(3): 265-268. Hasslung, F. C., M. Berg, G. M. Allan, B. M. Meehan, F. McNeilly and C. Fossum (2003). Identification of a sequence from the genome of porcine circovirus type 2 with an inhibitory effect on IFN-alpha production by porcine PBMCs. J Gen Virol 84(Pt 11): 2937-2945. Henney, C. S., K. Kuribayashi, D. E. Kern and S. Gillis (1981). Interleukin-2 augments natural killer cell activity. Nature 291(5813): 335-338. Horlen, K. P., S. S. Dritz, J. C. Nietfeld, S. C. Henry, R. A. Hesse, R. Oberst, M. Hays, J. Anderson and R. R. Rowland (2008). A field evaluation of mortality rate and growth performance in pigs vaccinated against porcine circovirus type 2. J Am Vet Med Assoc 232(6): 906-912. Juhan, N. M., T. LeRoith, T. Opriessnig and X. J. Meng (2010). The open reading frame 3 (ORF3) of porcine circovirus type 2 (PCV2) is dispensable for virus infection but evidence of reduced pathogenicity is limited in pigs infected by an ORF3-null PCV2 mutant. Virus Res 147(1): 60-66. Kekarainen, T., K. McCullough, M. Fort, C. Fossum, J. Segales and G. M. Allan (2010). Immune responses and vaccine-induced immunity against Porcine circovirus type 2. Vet Immunol Immunopathol 136(3-4): 185-193. Kim, D., C. H. Kim, K. Han, H. W. Seo, Y. Oh, C. Park, I. Kang and C. Chae (2011). Comparative efficacy of commercial Mycoplasma hyopneumoniae and porcine circovirus 2 (PCV2) vaccines in pigs experimentally infected with M. hyopneumoniae and PCV2. Vaccine 29(17): 3206-3212. Kim, J. and C. Chae (2001). Optimized protocols for the detection of porcine circovirus 2 DNA from. J Virol Methods 92(2): 105-111. Kim, J. and C. Chae (2003). Expression of monocyte chemoattractant protein-1 but not interleukin-8 in granulomatous lesions in lymph nodes from pigs with naturally occurring postweaning multisystemic wasting syndrome. Vet Pathol 40(2): 181-186. Krakowka, S., J. Ellis, F. McNeilly, C. Waldner and G. Allan (2005). Features of porcine circovirus-2 disease: correlations between lesions, amount and distribution of virus, and clinical outcome. J Vet Diagn Invest 17(3): 213-222. Krakowka, S., J. A. Ellis, F. McNeilly, S. Ringler, D. M. Rings and G. Allan (2001). Activation of the immune system is the pivotal event in the production of wasting disease in pigs infected with porcine circovirus-2 (PCV-2). Vet Pathol 38(1): 31-42. Krakowka, S., J. A. Ellis, B. Meehan, S. Kennedy, F. McNeilly and G. Allan (2000). Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet Pathol 37(3): 254-263. Lefebvre, D. J., P. Meerts, S. Costers, G. Misinzo, F. Barbe, K. Van Reeth and H. J. Nauwynck (2008). Increased porcine circovirus type 2 replication in porcine leukocytes in vitro and in vivo by concanavalin A stimulation. Vet Microbiol 132(1-2): 74-86. Lin, C. M., C. R. Jeng, H. W. Chang, I. C. Guo, Y. L. Huang, Y. C. Tsai, M. Y. Chia and V. F. Pang (2008). Characterization of porcine circovirus type 2 (PCV2) infection in swine lymphocytes using mitogen-stimulated peripheral blood lymphocytes from healthy PCV2-carrier pigs. Vet Immunol Immunopathol 124(3-4): 355-366. Lin, C. M., C. R. Jeng, S. H. Hsiao, Y. Lee, Y. C. Tsai, M. Y. Chia and V. F. Pang (2012). Monocyte-derived dendritic cells enhance cell proliferation and porcine circovirus type 2 replication in concanavalin A-stimulated swine peripheral blood lymphocytes in vitro. Vet Immunol Immunopathol 145(1-2): 368-378. Lin, C. M., C. R. Jeng, S. H. Hsiao, J. P. Liu, C. C. Chang, M. T. Chiou, Y. C. Tsai, M. Y. Chia and V. F. Pang (2011). Immunopathological characterization of porcine circovirus type 2 infection-associated follicular changes in inguinal lymph nodes using high-throughput tissue microarray. Vet Microbiol 149(1-2): 72-84. Lin, C. M., C. R. Jeng, J. P. Liu, E. C. Lin, C. C. Chang, Y. L. Huang, Y. C. Tsai, M. Y. Chia, C. H. Wan and V. F. Pang (2013). Immune gene expression profiles in swine inguinal lymph nodes with different viral loads of porcine circovirus type 2. Vet Microbiol 162(2-4): 519-529. Liu, J., I. Chen, Q. Du, H. Chua and J. Kwang (2006). The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo. J Virol 80(10): 5065-5073. Mandrioli, L., G. Sarli, S. Panarese, S. Baldoni and P. S. Marcato (2004). Apoptosis and proliferative activity in lymph node reaction in postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 97(1-2): 25-37. McIntosh, K. A., A. Tumber, J. C. Harding, S. Krakowka, J. A. Ellis and J. E. Hill (2009). Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues. Vet Microbiol 133(1-2): 23-33. McNeilly, F., D. Kennedy S Fau - Moffett, B. M. Moffett D Fau - Meehan, J. C. Meehan Bm Fau - Foster, E. G. Foster Jc Fau - Clarke, J. A. Clarke Eg Fau - Ellis, D. M. Ellis Ja Fau - Haines, B. M. Haines Dm Fau - Adair, G. M. Adair Bm Fau - Allan and G. M. Allan (1999). A comparison of in situ hybridization and immunohistochemistry for the detection. J Virol Methods 80(2): 123-128. Meerts, P., G. Misinzo, D. Lefebvre, J. Nielsen, A. Botner, C. S. Kristensen and H. J. Nauwynck (2006). Correlation between the presence of neutralizing antibodies against porcine circovirus 2 (PCV2) and protection against replication of the virus and development of PCV2-associated disease. BMC Vet Res 2: 6. Meerts, P., S. Van Gucht, E. Cox, A. Vandebosch and H. J. Nauwynck (2005). Correlation between type of adaptive immune response against porcine circovirus type 2 and level of virus replication. Viral Immunol 18(2): 333-341. Minami, Y., T. Kono, T. Miyazaki and T. Taniguchi (1993). The IL-2 receptor complex: its structure, function, and target genes. Annu Rev Immunol 11: 245-268. Nauwynck, H. J., R. Sanchez, P. Meerts, D. J. Lefebvre, D. Saha, L. Huang and G. Misinzo (2012). Cell tropism and entry of porcine circovirus 2. Virus Res 164(1-2): 43-45. Nielsen, J., I. E. Vincent, A. Botner, A. S. Ladekaer-Mikkelsen, G. Allan, A. Summerfield and K. C. McCullough (2003). Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 92(3-4): 97-111. Opriessnig, T., M. Fenaux, S. Yu, R. B. Evans, D. Cavanaugh, J. M. Gallup, F. J. Pallares, E. L. Thacker, K. M. Lager, X. J. Meng and P. G. Halbur (2004). Effect of porcine parvovirus vaccination on the development of PMWS in segregated early weaned pigs coinfected with type 2 porcine circovirus and porcine parvovirus. Vet Microbiol 98(3-4): 209-220. Opriessnig, T., P. G. Halbur, S. Yu, E. L. Thacker, M. Fenaux and X. J. Meng (2006). Effects of the timing of the administration of Mycoplasma hyopneumoniae bacterin on the development of lesions associated with porcine circovirus type 2. Vet Rec 158(5): 149-154. Opriessnig, T. and I. Langohr (2013). Current state of knowledge on porcine circovirus type 2-associated lesions. Vet Pathol 50(1): 23-38. Opriessnig, T., X. J. Meng and P. G. Halbur (2007). Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Invest 19(6): 591-615. Opriessnig, T., S. Ramamoorthy, D. M. Madson, A. R. Patterson, N. Pal, S. Carman, X. J. Meng and P. G. Halbur (2008). Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection. J Gen Virol 89(Pt 10): 2482-2491. Opriessnig, T., E. L. Thacker, S. Yu, M. Fenaux, X. J. Meng and P. G. Halbur (2004). Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Vet Pathol 41(6): 624-640. Ortaldo Jr Fau - Mason, A. T., J. P. Mason At Fau - Gerard, L. E. Gerard Jp Fau - Henderson, W. Henderson Le Fau - Farrar, R. F. Farrar W Fau - Hopkins, 3rd, R. B. Hopkins Rf 3rd Fau - Herberman, H. Herberman Rb Fau - Rabin and H. Rabin (1984). Effects of natural and recombinant IL 2 on regulation of IFN gamma production and. J Immunol 133(2): 779-783. Ostanello, F., A. Caprioli, A. Di Francesco, M. Battilani, G. Sala, G. Sarli, L. Mandrioli, F. McNeilly, G. M. Allan and S. Prosperi (2005). Experimental infection of 3-week-old conventional colostrum-fed pigs with porcine circovirus type 2 and porcine parvovirus. Vet Microbiol 108(3-4): 179-186. Paliard, X., R. de Waal Malefijt, H. Yssel, D. Blanchard, I. Chretien, J. Abrams, J. de Vries and H. Spits (1988). Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones. J Immunol 141(3): 849-855. Pallares, F. J., P. G. Halbur, T. Opriessnig, S. D. Sorden, D. Villar, B. H. Janke, M. J. Yaeger, D. J. Larson, K. J. Schwartz, K. J. Yoon and L. J. Hoffman (2002). Porcine circovirus type 2 (PCV-2) coinfections in US field cases of postweaning multisystemic wasting syndrome (PMWS). J Vet Diagn Invest 14(6): 515-519. Patterson, A. R. and T. Opriessnig (2010). Epidemiology and horizontal transmission of porcine circovirus type 2 (PCV2). Anim Health Res Rev 11(2): 217-234. Perez-Martin, E., A. Rovira, M. Calsamiglia, A. Mankertz, F. Rodriguez and J. Segales (2007). A new method to identify cell types that support porcine circovirus type 2 replication in formalin-fixed, paraffin-embedded swine tissues. J Virol Methods 146(1-2): 86-95. Pogranichniy, R. M., K. J. Yoon, P. A. Harms, S. D. Sorden and M. Daniels (2002). Case-control study on the association of porcine circovirus type 2 and other swine viral pathogens with postweaning multisystemic wasting syndrome. J Vet Diagn Invest 14(6): 449-456. Pogranichnyy, R. M., K. J. Yoon, P. A. Harms, S. L. Swenson, J. J. Zimmerman and S. D. Sorden (2000). Characterization of immune response of young pigs to porcine circovirus type 2 infection. Viral Immunol 13(2): 143-153. Quintana, J., J. Segales, C. Rosell, M. Calsamiglia, G. M. Rodriguez-Arrioja, F. Chianini, J. M. Folch, J. Maldonado, M. Canal, J. Plana-Duran and M. Domingo (2001). Clinical and pathological observations on pigs with postweaning multisystemic wasting syndrome. Vet Rec 149(12): 357-361. Ramamoorthy, S. and X. J. Meng (2009). Porcine circoviruses: a minuscule yet mammoth paradox. Anim Health Res Rev 10(1): 1-20. Rodriguez-Arrioja, G. M., J. Segales, M. Calsamiglia, A. R. Resendes, M. Balasch, J. Plana-Duran, J. Casal and M. Domingo (2002). Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome. Am J Vet Res 63(3): 354-357. Rosell, C., J. Segales, J. Plana-Duran, M. Balasch, G. M. Rodriguez-Arrioja, S. Kennedy, G. M. Allan, F. McNeilly, K. S. Latimer and M. Domingo (1999). Pathological, immunohistochemical, and in-situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J Comp Pathol 120(1): 59-78. Sanchez, R. E., Jr., P. Meerts, H. J. Nauwynck and M. B. Pensaert (2003). Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life. Vet Microbiol 95(1-2): 15-25. Sarli, G., L. Mandrioli, M. Laurenti, L. Sidoli, C. Cerati, G. Rolla and P. S. Marcato (2001). Immunohistochemical characterisation of the lymph node reaction in pig post-weaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 83(1-2): 53-67. Segales, J. (2012). Porcine circovirus type 2 (PCV2) infections: clinical signs, pathology and laboratory diagnosis. Virus Res 164(1-2): 10-19. Segales, J., G. M. Allan and M. Domingo (2005). Porcine circovirus diseases. Anim Health Res Rev 6(2): 119-142. Segales, J., F. Alonso, C. Rosell, J. Pastor, F. Chianini, E. Campos, L. Lopez-Fuertes, J. Quintana, G. Rodriguez-Arrioja, M. Calsamiglia, J. Pujols, J. Dominguez and M. Domingo (2001). Changes in peripheral blood leukocyte populations in pigs with natural postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 81(1-2): 37-44. Segales, J., C. Rosell and M. Domingo (2004). Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. Vet Microbiol 98(2): 137-149. Shibahara, T., K. Sato, Y. Ishikawa and K. Kadota (2000). Porcine circovirus induces B lymphocyte depletion in pigs with wasting disease syndrome. J Vet Med Sci 62(11): 1125-1131. Sipos, W., J. C. Duvigneau, M. Willheim, F. Schilcher, R. T. Hartl, G. Hofbauer, B. Exel, P. Pietschmann and F. Schmoll (2004). Systemic cytokine profile in feeder pigs suffering from natural postweaning multisystemic wasting syndrome (PMWS) as determined by semiquantitative RT-PCR and flow cytometric intracellular cytokine detection. Vet Immunol Immunopathol 99(1-2): 63-71. Steiner, E., C. Balmelli, H. Gerber, A. Summerfield and K. McCullough (2009). Cellular adaptive immune response against porcine circovirus type 2 in subclinically infected pigs. BMC Vet Res 5: 45. Stevenson, G. W., M. Kiupel, S. K. Mittal, J. Choi, K. S. Latimer and C. L. Kanitz (2001). Tissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors. J Vet Diagn Invest 13(1): 57-62. Takeda, K., T. Kaisho and S. Akira (2003). Toll-like receptors. Annu Rev Immunol 21: 335-376. Tischer, I., W. Mields, D. Wolff, M. Vagt and W. Griem (1986). Studies on epidemiology and pathogenicity of porcine circovirus. Arch Virol 91(3-4): 271-276. Tischer I Fau - Rasch, R., G. Rasch R Fau - Tochtermann and G. Tochtermann (1974). Characterization of papovavirus-and picornavirus-like particles in permanent pig. Zentralbl Bakteriol Orig A 226(2): 153-167. Tomas, A., L. T. Fernandes, A. Sanchez and J. Segales (2010). Time course differential gene expression in response to porcine circovirus type 2 subclinical infection. Vet Res 41(1): 12. Tomas, A., L. T. Fernandes, O. Valero and J. Segales (2008). A meta-analysis on experimental infections with porcine circovirus type 2 (PCV2). Vet Microbiol 132(3-4): 260-273. Tsai, Y. C., C. R. Jeng, S. H. Hsiao, H. W. Chang, J. J. Liu, C. C. Chang, C. M. Lin, M. Y. Chia and V. F. Pang (2010). Porcine circovirus type 2 (PCV2) induces cell proliferation, fusion, and chemokine expression in swine monocytic cells in vitro. Vet Res 41(5): 60. Vincent, I. E., C. Balmelli, B. Meehan, G. Allan, A. Summerfield and K. C. McCullough (2007). Silencing of natural interferon producing cell activation by porcine circovirus type 2 DNA. Immunology 120(1): 47-56. Vincent, I. E., C. P. Carrasco, L. Guzylack-Piriou, B. Herrmann, F. McNeilly, G. M. Allan, A. Summerfield and K. C. McCullough (2005). Subset-dependent modulation of dendritic cell activity by circovirus type 2. Immunology 115(3): 388-398. Vincent, I. E., C. P. Carrasco, B. Herrmann, B. M. Meehan, G. M. Allan, A. Summerfield and K. C. McCullough (2003). Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus. J Virol 77(24): 13288-13300. Wallgren, P., F. Hasslung, G. Bergstrom, A. Linder, K. Belak, C. Hard af Segerstad, M. Stampe, B. Molander, T. Bjornberg Kallay, E. Norregard, C. J. Ehlorsson, M. Tornquist, C. Fossum, G. M. Allan and J. A. Robertsson (2004). Postweaning multisystemic wasting syndrome--PMWS. the first year with the disease in Sweden. Vet Q 26(4): 170-187. Yu, S., P. G. Halbur and E. Thacker (2009). Effect of porcine circovirus type 2 infection and replication on activated porcine peripheral blood mononuclear cells in vitro. Vet Immunol Immunopathol 127(3-4): 350-356. Yu, S., T. Opriessnig, P. Kitikoon, D. Nilubol, P. G. Halbur and E. Thacker (2007). Porcine circovirus type 2 (PCV2) distribution and replication in tissues and immune cells in early infected pigs. Vet Immunol Immunopathol 115(3-4): 261-272. Yu, S., A. Vincent, T. Opriessnig, S. Carpenter, P. Kitikoon, P. G. Halbur and E. Thacker (2007). Quantification of PCV2 capsid transcript in peripheral blood mononuclear cells (PBMCs) in vitro. Vet Microbiol 123(1-3): 34-42. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16806 | - |
dc.description.abstract | 由第二型豬環狀病毒(PCV2)所引起的豬環狀病毒相關疾病中,以離乳後多系統性消耗症候群(PMWS)所引起的致害最為嚴重,造成離乳豬嚴重生長遲緩、體重下降,甚至死亡,此病在養豬產業造成重大的經濟損失,根據文獻可知,病毒量對於PCV2感染的嚴重度有重要的影響。由於大部分PCV2的感染豬隻為亞臨床感染,而有關於亞臨床PCV2感染免疫狀態的研究仍極為有限,因此,本研究以周邊血液單核細胞為檢測對象,以活體外給予致裂原刺激及PCV2攻毒,模擬田間免疫活化及PCV2再暴露等狀況,探討含不同病毒量的亞臨床染豬隻的細胞存活率、增殖能力、PCV2含量及細胞激素變化,以進一步了解在亞臨床感染的條件下PCV2可能的免疫致病機制。此外,豬場的PCV2疫苗使用,亦可能會影響亞臨床PCV2感染豬隻的免疫狀態,故也將其列入討論。實驗結果顯示,若依病毒量將豬隻分為大於103 copies/μL高病毒量豬隻及小於103 copies/μL低病毒量豬隻兩群,可見在給予Con A或PCV2處理後,含高病毒量豬隻的細胞存活率皆較含低病毒量豬隻為低;但就細胞增殖能力而言,在所有處理組中皆可發現含低病毒量豬隻會高於含高病毒量豬隻,此外,亦可見無疫苗注射且含低病毒量豬隻以及有疫苗注射但含高病毒豬隻的細胞增殖指數有較高的表現。在病毒量方面,含高病毒量豬隻的數量在有給予疫苗注射豬群中較無疫苗注射豬群為低,且平均PCV2病毒量也較低;而有疫苗注射且含低病毒量豬隻,亦能夠將病毒量維持在相對低值;此外,亦發現PCV2病毒的增加與細胞增殖能力上升有正相關。在細胞激素方面,含高病毒量豬隻的IL-1β、IL-8及IL-10 mRNA表現量較含低病毒量豬隻有較高的趨勢,而且在PCV2處理組的表現量上會明顯上升,尤其是在含高病毒量豬隻;IL-4 mRNA表現量,同樣在高病毒量豬隻有較高的表現趨勢;含低及高病毒量豬隻的IL-2、IL-4及IFN-ϒ mRNA表現量,在給予Con A後均有增加的趨勢,但是PCV2處理組則與對照組無明顯差異;而有給予疫苗但含高病毒量豬隻的IL-1β、IL-8及IL-10 mRNA表現量均會增加,同時,其細胞增殖能力也會明顯上升。根據實驗結果推測,從亞臨床PCV2感染到出現PCVAD臨床症狀可能為一漸進性病程,在亞臨床感染豬隻的免疫系統雖尚能維持正常調節,但病毒量的增加可能造成較嚴重的炎症並進而引發組織病變以及免疫抑制。此外,疫苗的注射有助於減低病毒量,但是在給予疫苗注射後所出現的含高病毒量豬隻,則可能有較嚴重的組織發炎病變及免疫抑制的傾向,而較有利於PCV2的複製。 | zh_TW |
dc.description.abstract | Porcine circovirus type 2 (PCV2) infection induces porcine circovirus associated diseases (PCVAD) among which postweaning multisystemic wasting syndrome (PMWS) is the most serious, which causes severe growth retardation, weight loss, and death in weaned pigs leading to considerable economic loss in pig industry. It is known that PCV2 load determines the outcome of PCV2 infection. However, the majority of PCV2-infected pigs in the field are subclinical and information regarding the immune status of the subclinically PCV2-infected pigs is rather limited. Therefore, using peripheral blood mononuclear cells (PBMC) treated with mitogen and recall antigen in vitro to mimic the immune system activation by vaccination, infection with other pathogens or PCV2 re-exposure in the field situation and to evaluate the survival rate, proliferation ability, PCV2 load, and cytokine expression profiles in subclinically PCV2-infected pigs with different PCV2 loads to determine the immune status of the asymptomatic pigs may provide a more clear picture in understanding the immunopathogenesis of PCV2 infection in pigs. Due to the fact that PCV2 vaccine is widely used in the field, the effects of vaccination on the immune status of subclinically PCV2-infected pigs are discussed as well. According to the PCV2 loads, we subdivided the pigs into two groups, including the high viral load group (HVL) which had PCV2 genome number more than 103 copies/μL and the low viral load group (LVL) which had PCV2 genome number less than 103 copies/μL. In Con A or PCV2 treated groups, the result of cell viability of PBMC displayed that pigs with HVL had lower cell viability than those with LVL. In general, the proliferation abilities of PBMC in pigs with LVL were higher than pigs with HVL. Furthermore, non-vaccinated pigs with LVL and vaccinated pigs with HVL had higher cell proliferation abilities. In the result of PCV2 load, it showed that the vaccinated pigs had lower viral loads and only a relatively small number of pigs had high PCV2 loads. Besides, vaccinated pigs with LVL could maintain a low level of PCV2 load. Pigs with HVL were prone to have higher mRNA expression levels of IL-1β, IL-8, and IL-10, which were more prominent in PCV2-treated group, especially in the HVL group. The level of IL-4 mRNA expression in the HVL pigs was higher than those in pigs with LVL. The expression levels of IL-2, IL-4, and IFN-ϒ became higher when treated with Con A but they were not responsive to PCV2 re-exposure. Besides, the mRNA expression levels of IL-1β, IL-8, and IL-10 increased in vaccinated pigs with HVL and had increasing cell proliferation ability in PBMC. According to the results, it is considered that pigs transform from subclinical PCV2 infection to PCVAD might be a progressing course of disease development. In subclinically infected pigs, the immune system might regulate normally but inflammation and immunosuppression could be more severe since the increasing PCV2 viral loads. Although PCV2 vaccination would reduce the viral loads quite successfully, vaccinated pigs with high PCV2 loads might be prone to have more severe inflammation and immunosuppression and more conductive to PCV2 replication than non-vaccinated ones. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T23:46:47Z (GMT). No. of bitstreams: 1 ntu-103-R01644003-1.pdf: 1925974 bytes, checksum: 654cedb97fd91731b91004e8db8c34db (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 中文摘要 i
英文摘要 iii 目錄 v 表次 viii 圖次 ix 第一章 序言 1 第二章 文獻回顧 2 第一節 豬環狀病毒及其流行病學 2 第二節 豬環狀病毒相關症 3 2.1 豬環狀病毒的傳播路徑 3 2.2 豬環狀病毒相關疾病的致病機制及發病相關因子 4 2.3 豬環狀病毒相關疾病的臨床症狀及組織病變 6 2.4 豬環狀病毒相關疾病的診斷 8 第三節 豬環狀病毒的複製 8 第四節 第二型豬環狀病毒對豬隻免疫調控之影響 10 4.1第二型豬環狀病毒量與離乳後多系統性消耗症候群之影響 10 4.2 離乳後多系統性消耗症候群對於淋巴組織的影響 11 4.3 第二型豬環狀病毒感染對於後天免疫的影響 12 4.4 離乳後多系統性消耗症候群對豬隻細胞激素之改變 13 4.5 離乳後多系統性消耗症候群於基因表現之變化 15 4.6亞臨床第二型豬環狀病毒感染之影響 16 第三章 材料與方法 18 第一節 實驗設計 18 第二節 實驗材料 20 2-1 實驗動物與血液樣本 20 2-2 細胞培養基及試劑 21 2-3 核酸抽取試劑 23 2-4 即時聚合酶鏈鎖反應之商品化試劑 24 2-5 流式細胞儀相關試劑 26 2-6 儀器 27 第三節 實驗方法 27 3-1 周邊血液單核細胞的分離 27 3-2 第二型豬環狀病毒與Concanavalin A (Con A)對周邊血液單核細胞的影響 28 3-3 以Propidium iodine (PI)染色檢測細胞存活率 29 3-4 以CFSE染色檢測細胞增殖能力 29 3-5 細胞、血漿及上清液的第二型豬環狀病毒核酸抽取 30 3-6 細胞RNA抽取 31 3-7 反轉綠聚合酶鏈鎖反應 31 3-8 利用即時聚合酶鏈鎖反應檢測第二型豬環狀病毒量及細胞激素(IL-1β、IL-2、IL-4、IL-8、IL-10、IFN-ϒ) mRNA的表現量 32 3-9 統計分析 33 第四章 結果 34 第一節 豬隻樣本數及周邊血液單核細胞的純化 34 第二節 細胞存活率 34 第三節 細胞增殖能力 36 第四節 第二型豬環狀病毒量的變化 37 第五節 周邊血液單核細胞的細胞激素(IL-1β、IL-2、IL-4、IL-8、IL-10及IFN-ϒ) mRNA表現量變化 39 5.1 不同細胞收集時間點對周邊血液單核細胞的細胞激素mRNA表現量的影響 39 5.2 不同病毒量的周邊血液單核細胞在給予各種處理後細胞激素mRNA表現量的差異 41 5.3不同病毒量及疫苗注射與否對周邊血液單核細胞的細胞激素mRNA表現量的影響 43 第五章 討論 57 第六章 文獻參考 69 | |
dc.language.iso | zh-TW | |
dc.title | 亞臨床第二型豬環狀病毒感染豬的免疫狀態-血液病毒量對周邊血液單核細胞對致裂原及第二型豬環狀病毒刺激反應的影響 | zh_TW |
dc.title | The immune status of subclinically porcine circovirus type 2 (PCV2)-infected pigs – Effects of PCV2 loads on the functions of peripheral blood mononuclear cells in response to mitogen or PCV2 recall | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭謙仁 | |
dc.contributor.oralexamcommittee | 劉涓,張志成,張惠雯,黃有良 | |
dc.subject.keyword | 第二型豬環狀病毒,亞臨床感染,豬周邊血液單核細胞,豬環狀病毒相關症,疫苗注射, | zh_TW |
dc.subject.keyword | Porcine circovirus type 2,subclinical infection,porcine peripheral blood mononuclear cell,porcine circovirus associated disease,vaccination, | en |
dc.relation.page | 78 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2014-06-13 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 分子暨比較病理生物學研究所 | zh_TW |
顯示於系所單位: | 分子暨比較病理生物學研究所 |
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