請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16658
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳賢燁(Hsien-Yeh Chen) | |
dc.contributor.author | Hsin-Ying Ho | en |
dc.contributor.author | 何信穎 | zh_TW |
dc.date.accessioned | 2021-06-07T23:42:58Z | - |
dc.date.copyright | 2014-08-12 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-07-22 | |
dc.identifier.citation | 1 Romeas, V., Pichat, P., Guillard, C., Chopin, T. & Lehaut, C. Testing the Efficacy and the Potential Effect on Indoor Air Quality of a Transparent Self-Cleaning TiO2-Coated Glass through the Degradation of a Fluoranthene Layer. Industrial & Engineering Chemistry Research 38, 3878-3885, doi:10.1021/ie990326k (1999).
2 Balazs, A. C., Emrick, T. & Russell, T. P. Nanoparticle Polymer Composites: Where Two Small Worlds Meet. Science 314, 1107-1110, doi:10.1126/science.1130557 (2006). 3 Wang, M. & Thanou, M. Targeting nanoparticles to cancer. Pharmacological Research 62, 90-99, doi:http://dx.doi.org/10.1016/j.phrs.2010.03.005 (2010). 4 Matsumura, Y. & Maeda, H. A New Concept for Macromolecular Therapeutics in Cancer Chemotherapy: Mechanism of Tumoritropic Accumulation of Proteins and the Antitumor Agent Smancs. Cancer Research 46, 6387-6392 (1986). 5 Zhang, L. et al. Nanoparticles in Medicine: Therapeutic Applications and Developments. Clin Pharmacol Ther 83, 761-769 (2007). 6 Davis, M. E., Chen, Z. & Shin, D. M. Nanoparticle therapeutics: an emerging treatment modality for cancer. Nat Rev Drug Discov 7, 771-782 (2008). 7 Champion, J. A. & Mitragotri, S. Role of target geometry in phagocytosis. Proceedings of the National Academy of Sciences of the United States of America 103, 4930-4934, doi:10.1073/pnas.0600997103 (2006). 8 Chilkoti, A. & Hubbell, J. A. Biointerface Science. MRS Bulletin 30, 175-179 (2005). 9 Petros, R. A. & DeSimone, J. M. Strategies in the design of nanoparticles for therapeutic applications. Nat Rev Drug Discov 9, 615-627 (2010). 10 Tsai, M.-Y. et al. Vapor-based synthesis of maleimide-functionalized coating for biointerface engineering. Chemical Communications 48, 10969-10971, doi:10.1039/C2CC35892A (2012). 11 Senkevich, J. J. et al. Unique structure/properties of chemical vapor deposited parylene E. Journal of Vacuum Science & Technology A 20, 1445-1449, doi:doi:http://dx.doi.org/10.1116/1.1487870 (2002). 12 Duffy, D. C., McDonald, J. C., Schueller, O. J. A. & Whitesides, G. M. Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane). Analytical Chemistry 70, 4974-4984, doi:10.1021/ac980656z (1998). 13 Lahann, J. Vapor-based polymer coatings for potential biomedical applications. Polymer International 55, 1361-1370, doi:10.1002/pi.2098 (2006). 14 Ishaque, M., Agarwal, S. & Greiner, A. in e-Polymers Vol. 2 442 (2002). 15 Gilch, H. G. & Wheelwright, W. L. Polymerization of α-halogenated p-xylenes with base. Journal of Polymer Science Part A-1: Polymer Chemistry 4, 1337-1349, doi:10.1002/pol.1966.150040602 (1966). 16 Senkevich, J. J., Yang, G. R. & Lu, T. M. The facile surface modification of poly(p-xylylene) ultrathin films. Colloids and Surfaces A: Physicochemical and Engineering Aspects 216, 167-173, doi:http://dx.doi.org/10.1016/S0927-7757(02)00546-0 (2003). 17 Lahann, J. & Langer, R. Novel Poly(p-xylylenes): Thin Films with Tailored Chemical and Optical Properties. Macromolecules 35, 4380-4386, doi:10.1021/ma011769e (2002). 18 Lahann, J., Choi, I. S., Lee, J., Jensen, K. F. & Langer, R. A New Method toward Microengineered Surfaces Based on Reactive Coating. Angewandte Chemie International Edition 40, 3166-3169, doi:10.1002/1521-3773(20010903)40:17<3166::AID-ANIE3166>3.0.CO;2-# (2001). 19 Chen, H.-Y. & Lahann, J. Fabrication of Discontinuous Surface Patterns within Microfluidic Channels Using Photodefinable Vapor-Based Polymer Coatings. Analytical Chemistry 77, 6909-6914, doi:10.1021/ac050964e (2005). 20 Nandivada, H., Chen, H.-Y., Bondarenko, L. & Lahann, J. Reactive Polymer Coatings that “Click”. Angewandte Chemie International Edition 45, 3360-3363, doi:10.1002/anie.200600357 (2006). 21 Tenhaeff, W. E. & Gleason, K. K. Initiated and Oxidative Chemical Vapor Deposition of Polymeric Thin Films: iCVD and oCVD. Advanced Functional Materials 18, 979-992, doi:10.1002/adfm.200701479 (2008). 22 Suh, K. Y., Langer, R. & Lahann, J. A Novel Photodefinable Reactive Polymer Coating and Its Use for Microfabrication of Hydrogel Elements. Advanced Materials 16, 1401-1405, doi:10.1002/adma.200400101 (2004). 23 Jiang, X., Chen, H. Y., Galvan, G., Yoshida, M. & Lahann, J. Vapor-Based Initiator Coatings for Atom Transfer Radical Polymerization. Advanced Functional Materials 18, 27-35, doi:10.1002/adfm.200700789 (2008). 24 Qu, Z. et al. A Biologically Active Surface Enzyme Assembly that Attenuates Thrombus Formation. Advanced Functional Materials 21, 4736-4743, doi:10.1002/adfm.201101687 (2011). 25 Elkasabi, Y. & Lahann, J. Vapor-Based Polymer Gradients. Macromolecular Rapid Communications 30, 57-63, doi:10.1002/marc.200800578 (2009). 26 Lahann, J., Hocker, H. & Langer, R. Synthesis of Amino[2.2]paracyclophanes—Beneficial Monomers for Bioactive Coating of Medical Implant Materials. Angewandte Chemie International Edition 40, 726-728, doi:10.1002/1521-3773(20010216)40:4<726::AID-ANIE7260>3.0.CO;2-X (2001). 27 Lahann, J. et al. Reactive Polymer Coatings: A Platform for Patterning Proteins and Mammalian Cells onto a Broad Range of Materials. Langmuir 18, 3632-3638, doi:10.1021/la011464t (2002). 28 Bangham, A. D. & Horne, R. W. Negative staining of phospholipids and their structural modification by surface-active agents as observed in the electron microscope. Journal of Molecular Biology 8, 660-IN610, doi:http://dx.doi.org/10.1016/S0022-2836(64)80115-7 (1964). 29 Bangham, A. D., Standish, M. M. & Watkins, J. C. Diffusion of univalent ions across the lamellae of swollen phospholipids. Journal of Molecular Biology 13, 238-IN227, doi:http://dx.doi.org/10.1016/S0022-2836(65)80093-6 (1965). 30 Kataoka, K., Harada, A. & Nagasaki, Y. Block copolymer micelles for drug delivery: design, characterization and biological significance. Advanced Drug Delivery Reviews 47, 113-131, doi:http://dx.doi.org/10.1016/S0169-409X(00)00124-1 (2001). 31 Duncan, R. & Izzo, L. Dendrimer biocompatibility and toxicity. Advanced Drug Delivery Reviews 57, 2215-2237, doi:http://dx.doi.org/10.1016/j.addr.2005.09.019 (2005). 32 Wang, G. & Uludag, H. Recent developments in nanoparticle-based drug delivery and targeting systems with emphasis on protein-based nanoparticles. Expert Opinion on Drug Delivery 5, 499-515, doi:doi:10.1517/17425247.5.5.499 (2008). 33 Roy, I. et al. Ceramic-Based Nanoparticles Entrapping Water-Insoluble Photosensitizing Anticancer Drugs: A Novel Drug−Carrier System for Photodynamic Therapy. Journal of the American Chemical Society 125, 7860-7865, doi:10.1021/ja0343095 (2003). 34 Paciotti, G. F. et al. Colloidal Gold: A Novel Nanoparticle Vector for Tumor Directed Drug Delivery. Drug Delivery 11, 169-183, doi:doi:10.1080/10717540490433895 (2004). 35 Davis, M. E. The First Targeted Delivery of siRNA in Humans via a Self-Assembling, Cyclodextrin Polymer-Based Nanoparticle: From Concept to Clinic. Molecular Pharmaceutics 6, 659-668, doi:10.1021/mp900015y (2009). 36 Jatzkewitz, H. [Incorporation of physiologically-active substances into a colloidal blood plasma substitute. I. Incorporation of mescaline peptide into polyvinylpyrrolidone]. Hoppe-Seyler's Zeitschrift fur physiologische Chemie 297, 149-156 (1954). 37 Scheffel, U., Rhodes, B. A., Natarajan, T. K. & Wagner, H. N., Jr. Albumin microspheres for study of the reticuloendothelial system. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 13, 498-503 (1972). 38 Kreuter, J. Nanoparticles—a historical perspective. International Journal of Pharmaceutics 331, 1-10, doi:http://dx.doi.org/10.1016/j.ijpharm.2006.10.021 (2007). 39 Gradishar, W. J. et al. Phase III Trial of Nanoparticle Albumin-Bound Paclitaxel Compared With Polyethylated Castor Oil–Based Paclitaxel in Women With Breast Cancer. Journal of Clinical Oncology 23, 7794-7803, doi:10.1200/jco.2005.04.937 (2005). 40 Maeda, H., Greish, K. & Fang, J. in Polymer Therapeutics II Vol. 193 Advances in Polymer Science (eds Ronit Satchi-Fainaro & Ruth Duncan) Ch. 26, 103-121 (Springer Berlin Heidelberg, 2006). 41 Brem, H. et al. Placebo-controlled trial of safety and efficacy of intraoperative controlled delivery by biodegradable polymers of chemotherapy for recurrent gliomas. The Lancet 345, 1008-1012, doi:http://dx.doi.org/10.1016/S0140-6736(95)90755-6 (1995). 42 Bock, N., Dargaville, T. R. & Woodruff, M. A. Electrospraying of polymers with therapeutic molecules: State of the art. Progress in Polymer Science 37, 1510-1551, doi:http://dx.doi.org/10.1016/j.progpolymsci.2012.03.002 (2012). 43 Xu, Y., Skotak, M. & Hanna, M. Electrospray encapsulation of water-soluble protein with polylactide. I. Effects of formulations and process on morphology and particle size. Journal of Microencapsulation 23, 69-78 (2006). 44 Hong, Y., Li, Y., Yin, Y., Li, D. & Zou, G. Electrohydrodynamic atomization of quasi-monodisperse drug-loaded spherical/wrinkled microparticles. Journal of Aerosol Science 39, 525-536 (2008). 45 Valo, H. et al. Electrospray encapsulation of hydrophilic and hydrophobic drugs in poly(L-lactic acid) nanoparticles. Small 5, 1791-1798 (2009). 46 Xie, J., Marijnissen, J. C. M. & Wang, C. H. Microparticles developed by electrohydrodynamic atomization for the local delivery of anticancer drug to treat C6 glioma in vitro. Biomaterials 27, 3321-3332 (2006). 47 Xu, Y. & Hanna, M. A. Electrospray encapsulation of water-soluble protein with polylactide. Effects of formulations on morphology, encapsulation efficiency and release profile of particles. International Journal of Pharmaceutics 320, 30-36 (2006). 48 Xu, Y. & Hanna, M. A. Electrosprayed bovine serum albumin-loaded tripolyphosphate cross-linked chitosan capsules: Synthesis and characterization. Journal of Microencapsulation 24, 143-151 (2007). 49 Arya, N., Chakraborty, S., Dube, N. & Katti, D. S. Electrospraying: A facile technique for synthesis of chitosan-based micro/nanospheres for drug delivery applications. Journal of Biomedical Materials Research - Part B Applied Biomaterials 88, 17-31 (2009). 50 Enayati, M., Ahmad, Z., Stride, E. & Edirisinghe, M. Size mapping of electric field-assisted production of polycaprolactone particles. Journal of the Royal Society, Interface / the Royal Society 7 Suppl 4, S393-402, doi:10.1098/rsif.2010.0099.focus (2010). 51 Xie, J., Lim, L. K., Phua, Y., Hua, J. & Wang, C. H. Electrohydrodynamic atomization for biodegradable polymeric particle production. Journal of Colloid and Interface Science 302, 103-112 (2006). 52 Bock, N., Woodruff, M. A., Hutmacher, D. W. & Dargaville, T. R. Electrospraying, a Reproducible Method for Production of Polymeric Microspheres for Biomedical Applications. Polymers 3, 131-149 (2011). 53 Gupta, P., Elkins, C., Long, T. E. & Wilkes, G. L. Electrospinning of linear homopolymers of poly(methyl methacrylate): Exploring relationships between fiber formation, viscosity, molecular weight and concentration in a good solvent. Polymer 46, 4799-4810 (2005). 54 Roh, K.-H., Martin, D. C. & Lahann, J. Biphasic Janus particles with nanoscale anisotropy. Nat Mater 4, 759-763, doi:http://www.nature.com/nmat/journal/v4/n10/suppinfo/nmat1486_S1.html (2005). 55 Roh, K.-H., Martin, D. C. & Lahann, J. Triphasic Nanocolloids. Journal of the American Chemical Society 128, 6796-6797, doi:10.1021/ja060836n (2006). | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16658 | - |
dc.description.abstract | 微奈米粒子技術的成熟賦予了許多材料截然不同的性質,而在工業上的應用也漸趨豐富,此外,近年來生物技術以及奈米醫藥的發展獲得許多重要的突破以及成功的應用,而在這其中微奈米技術的參與更是不可或缺,然而,在廣大的高分子領域當中,功能性聚對二甲苯高分子微奈米粒子 (functionalized poly-para-xylylene particle) 首次利用電噴霧製程技術 (electrospraying technique) 由苯甲醯基聚對二甲苯 (poly[(4-benzoyl-p-xylylene)-co-(p-xylylene)]) 成功地製備出來。一系列的分析包括動態光散射 (dynamic light scattering) 以及介面電位 (zeta potential) 將被用來驗證粒子之粒徑及表面電荷,掃描式電子顯微鏡 (scanning electron microscope) 以及穿透式電子顯微鏡 (transmittance electron microscope) 將被用來分析粒子表面型態以及內部構造,傅立葉轉換紅外線光譜 (fourier transform infrared spectroscope) 將被用來分析粒子化學組成,共軛焦螢光顯微鏡 (confocal fluorescence microscope) 將被用來擷取螢光影像,石英晶體微天平 (quartz crystal microbalance) 將被用來測量粒子之重量。在成功製備出苯甲醯基聚對二甲苯微奈米粒子後,polyethylene glycol-biotin 率先修飾於粒子表面,接著以生物耦合技術專一性地將 Alexa Fluor 488 streptavidin 透過 biotin-streptavidin作用而結合,藉此證明表面官能基活性;而將粒子改質使具有生物功能性前亦進行細胞毒性測試,並以 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) 進行細胞存活率分析,最後,此一功能性聚對二甲苯微奈米粒子將被設計並且改質以展示不同之生物功能,其一為將奈米粒子表面修飾有抗癌藥物 paclitaxel 進行癌細胞毒殺測試,其二為將奈米粒子表面修飾有抗菌藥物 chlorhexidine 進行最小抑菌濃度測試。未來除了苯甲醯基聚對二甲苯外,各式各樣具有不同功能性的聚對二甲苯亦能夠以微奈米粒子的形式進行多樣的應用,更重要的是,透過製程變化能夠使微奈米粒子由單功能性進化成多功能性以滿足漸趨複雜之改質設計,然而,不同功能性的官能基於粒子表面的分布亦能夠以混合或者區域性的方式呈現,藉此提升微奈米粒子改質設計的層次。 | zh_TW |
dc.description.abstract | Nanotechnology has led numerous materials to have their brand new properties and those materials have been widely applied in industry. Besides, with the help of nanotechnology, the field of biotechnology and nanomedicine has gain many important breakthrough. However, in the field of macromolecules, functionalized poly-para-xylylene particle was first successfully manufactured by electrospraying technique with poly[(4-benzoyl-p-xylylene)-co-(p-xylylene)], benzoyl-PPX. Dynamic light scattering and zeta potential tests were performed to measure the diameter and surface charge of vary-sized micro- to nano- particle. Scanning electron microscope and transmittance electron microscope were used to make sure the surface morphology and the interior structure of benzoyl-PPX particles. Fourier transform infrared spectroscope was also performed to analyze the particle chemistry characteristics. Confocal fluorescence microscope was use as a tool to get fluorescence photo of particle. Quartz crystal microbalance was used to weight the particle powder. Polyethylene glycol-biotin was first attach to particle by UV light induced photochemistry reaction, then fluorescence Alexa Fluor 488 streptavidin was combined with PEG-biotin through bioconjugation reaction specifically. Before bio-test to be conduct, particles of varying size were used to undergo cytotoxicity test followed by MTT assay. Finally, this functionalized poly[(4-benzoyl-p-xylylene)-co-(p-xylylene)] particle was design to exhibit distinct biological functions by first immobilizing anti-cancer drug paclitaxel and serving as cancer cell killer. Secondly, anti-bacteria drug chlorhexidine was also immobilized onto particle surface to suppress the growth of bacteria. In the future, many kinds of functionalized poly-para-xylylene particle in addition to benzoyl-PPX particle will have different applications. More importantly, mono-functionalized particle can turn into multi-functionalized particle with the adjustment of electrospraying technique. However, the different functional groups can also be distribute in the way of mixed or biphasic on the surface of particle, which will greatly meet the requirement of more complex particle design. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T23:42:58Z (GMT). No. of bitstreams: 1 ntu-103-R01524056-1.pdf: 24250311 bytes, checksum: 4316c13066d905cf1f05f37e5ed08dbd (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 致謝................................................................................................................................ I
摘要............................................................................................................................... II Abstract ........................................................................................................................ IV 目錄.............................................................................................................................. VI 圖目錄.......................................................................................................................... IX 表目錄........................................................................................................................ XII 第一章 緒論.................................................................................................................. 1 1.1 前言 ...................................................................................................................... 1 1.2 文獻回顧 .............................................................................................................. 3 1.2.1 功能性聚對二甲苯高分子 ........................................................................... 3 1.2.2 微奈米粒子之生物醫學應用 ....................................................................... 6 1.2.3 電噴霧製程技術 ........................................................................................... 8 1.3 研究動機 .............................................................................................................. 9 第二章 實驗步驟........................................................................................................ 11 2.1 藥品 .................................................................................................................... 11 2.2 儀器 .................................................................................................................... 12 2.2.1 熱蒸鍍設備 ................................................................................................. 12 2.2.2 傅立葉轉換紅外線光譜儀 ......................................................................... 13 2.2.3 掃描式電子顯微鏡 ..................................................................................... 14 2.2.4 穿透式電子顯微鏡 ..................................................................................... 15 2.2.5 動態光散射儀 ............................................................................................. 16 2.2.6 介面電位分析儀 ......................................................................................... 16 2.2.7 共軛焦螢光顯微鏡 ..................................................................................... 17 VII 2.2.8 石英晶體微天平 ......................................................................................... 18 2.3 苯甲醯基對二甲苯二聚體之製備 .................................................................... 19 2.4 化學氣相沉積製程技術製備苯甲醯基聚對二甲苯高分子 ............................ 20 2.5 電噴霧製程技術製備苯甲醯基聚對二甲苯微奈米粒子 ................................ 22 2.5.1 電噴霧製程設備 ......................................................................................... 22 2.5.2 電噴霧製程技術 ......................................................................................... 27 2.6 苯甲醯基聚對二甲苯微奈米粒子表面改質及應用技術 ................................ 29 2.6.1 生物耦合技術 ............................................................................................ 29 2.6.2 細胞毒性測試 ............................................................................................. 29 2.6.3 抗癌功能之改質 ......................................................................................... 30 2.6.4 癌細胞毒性測試 ......................................................................................... 30 2.6.5 癌細胞奈米粒子胞吞測試 ......................................................................... 31 2.6.6 抗菌功能之改質 ......................................................................................... 31 2.6.7 最小抑菌濃度測試 ..................................................................................... 32 第三章 結果討論........................................................................................................ 33 3.1 苯甲醯基對二甲苯二聚體之特性分析 ............................................................ 33 3.2 苯甲醯基聚對二甲苯高分子之特性分析 ........................................................ 34 3.3 電噴霧製程技術之參數探討 ............................................................................ 36 3.4 苯甲醯基聚對二甲苯微奈米粒子之特性分析 ................................................ 45 3.4.1 動態光散射儀圖譜分析 ............................................................................. 45 3.4.2 介面電位分析 ............................................................................................. 45 3.4.3 掃描式電子顯微鏡圖像分析 ..................................................................... 48 3.4.4 穿透式電子顯微鏡圖像分析 ..................................................................... 52 3.4.5 傅立葉轉換紅外線光譜分析 ..................................................................... 55 3.5 苯甲醯基聚對二甲苯微奈米粒子之應用 ........................................................ 57 VIII 3.5.1 生物耦合技術 ............................................................................................. 57 3.5.2 細胞毒性測試 ............................................................................................. 60 3.5.3 抗癌功能之應用 ......................................................................................... 62 3.5.4 抗菌功能之應用 ......................................................................................... 68 第四章 結論與未來展望............................................................................................ 75 4.1 結論 .................................................................................................................... 75 4.2 未來展望 ............................................................................................................ 77 參考文獻...................................................................................................................... 89 附錄.............................................................................................................................. 97 | |
dc.language.iso | zh-TW | |
dc.title | 電噴霧製程技術製備功能性聚對二甲苯微奈米粒子之開發應用 | zh_TW |
dc.title | Micro- and Nano- Particles Based on Functionalized Poly-para-xylylenes via Electrospraying | en |
dc.type | Thesis | |
dc.date.schoolyear | 102-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡偉博(Wei-Bor Tsai),吳嘉文(Chia-Wen Wu),姜昱至(Yu-Chih Chiang) | |
dc.subject.keyword | 電噴霧製程技術,功能性聚對二甲苯,微奈米粒子,生物耦合技術,表面改質, | zh_TW |
dc.subject.keyword | electrospraying technique,functionalized poly-para-xylylene,micro- to nano- particle,bioconjugation,surface modification, | en |
dc.relation.page | 97 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2014-07-22 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 23.68 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。