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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳昭瑩 | |
| dc.contributor.author | Ming-Wei Chang | en |
| dc.contributor.author | 張明偉 | zh_TW |
| dc.date.accessioned | 2021-06-07T18:22:00Z | - |
| dc.date.copyright | 2012-01-16 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-11-17 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16582 | - |
| dc.description.abstract | 抗菌胜肽具有抗菌活性，由12~50 個胺基酸組成，廣泛存在於各種生物體如人類、昆蟲、植物、細菌等，可以協助生物體抵抗細菌、真菌、病毒、寄生蟲等各種病原感染。LsGRP1 (Lilium ‘Star Gazer’ glycine-rich protein)是一源自於葵百合的防禦相關蛋白，由138 個胺基酸組成；C 端序列的16%為半胱胺酸(cysteine)，具有良好的抑菌能力，為一抗菌胜肽。為了解LsGRP1 的應用潛力，測試其對臺灣多種常見植物病原菌的抑制作用，發現LsGRP1 的C 端胜肽(LsGRP1C)可有效抑制Alternaria brassicicola、Botrytis cinerea 與Colletotrichum acutatum 等真菌孢子發芽，對Pseudomonas syringae pv. syringae、Xanthomonas campestris pv.vesicatoria 與Clavibacter michiganensis 等細菌則有致死的作用。於植體上，LsGRP1C 可在甘藍葉片及草莓花瓣分別抑制黑斑病及灰黴病的發展，並可抑制P. syringae pv. syringae 在番茄葉片上的感染。進一步探討LsGRP1C 的抑菌機制，以掃描式電子顯微鏡觀察細菌細胞外部形態的變化，發現處理LsGRP1C 的細菌表面有疣狀凸起並於細菌周圍出現許多球狀物；藉由螢光染劑SYTOX green 觀察LsGRP1C 對細菌細胞的影響，發現此抗菌胜肽可造成細菌細胞膜穿孔。 | zh_TW |
| dc.description.abstract | Antimicrobial peptides (AMPs) are a class of small host defense peptides, normally 12 to 50 amino acids, with antimicrobial activity against bacteria, fungi, viruses, parasites and other pathogenic microorganisms on humans, insects, plants, etc. LsGRP1 (Lilium ‘Star Gazer’ glycine-rich protein) is a plant defense-related protein derived from Lilium, consisting of 138 amino acids with 16% cysteine in C-terminal region. Antimicrobial activity of the synthetic C-terminal LsGRP1 (LsGRP1C) had been shown previously. To understand the potential applications of this peptide, its inhibitory abilities to a variety of plant pathogens were assayed and found to inhibit spore germination of Alternaria brassicicola, Botrytis cinerea, and Colletotrichum acutatum. In addition, this peptide caused bacterial cell death in Pseudomonas syringae pv. syringae, Xanthomonas campestris pv. vesicatoria, and Clavibacter michiganensis., In planta assays showed that this synthetic LsGRP1C inhibited symptom developments of cabbage black spot, strawberry gray mold, and tomato bacterial leaf spot. For investigation of the antibacterial mechanism, bacterial cell surface was examined by scanning electron microscopy, and the results showed that LsGRP1C caused protrusion of bacterial surface and formation of spherical bodies aside of bacterial cells. Examination with fluorescent dye SYTOX green showed that this AMP could increase membrane permeability of the target bacterial cells. | en |
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| dc.description.tableofcontents | 壹、中文摘要 1
貳、英文摘要 2 参、前 言 4 肆、前人研究 6 一、 植物之富含甘胺酸蛋白(glycine-rich protein, GRP) 6 二、 抗菌胜肽的結構及理化特性 6 三、 抗菌胜肽在農業上的應用 7 四、 抗菌胜肽之抗生機制 9 五、 病害防治標的 12 六、 酵母菌蛋白質表現系統 13 伍、材料與方法 15 一、 真菌菌株培養與保存 15 二、 細菌菌株培養與保存 15 三、 酵母菌菌株培養與保存 16 四、 人工合成LsGRP1 C端胜肽 (LsGRP1C) 16 五、 植物栽植與處理 16 六、 LsGRP1C抑制細菌與酵母菌能力測試 17 七、 LsGRP1C抑制真菌能力試驗 17 八、 以掃描式電子顯微鏡觀察LsGRP1C對細菌之作用 18 九、 植物體上LsGRP1C抑制Alternaria brassicicola試驗 18 十、 以染色觀察葉片上真菌之侵入情形 19 十一、 植體上LsGRP1C抑制Botrytis cinerea試驗 19 十二、 LsGRP 1C之細胞毒性試驗 20 十三、 藉由螢光染劑SYTOX green觀察LsGRP1C對細菌與酵母菌細胞膜之影響 20 十四、 以酵母菌表現LsGRP1C之分子選殖 20 1. 細菌質體DNA之小量製備法 20 2. DNA瓊脂精膠體電泳分析 21 3. 引子設計 21 4. 聚合酵素連鎖反應 22 5. TA cloning 22 6. 大腸桿菌勝任細胞製備 22 7. 大腸桿菌細胞轉形 23 8. 大腸桿菌轉形株之篩選 23 9. 限制酵素切割 23 10. DNA片段的回收與純化 24 11. DNA黏接反應 24 12. 酵母菌勝任細胞製備 25 13. 酵母菌細胞轉形 25 14. 酵母菌菌落聚合酵素連鎖反應之增幅篩選 25 十五、 利用酵母菌表現LsGRP1C 26 1. 胞內蛋白質萃取 26 2. Tricine-SDS-聚丙烯醯胺膠體分析 27 3. 酵母菌表現LsGRP1C蛋白質產物純化流程 28 4. 西方墨點法分析(western blot)及抗體偵測 28 陸、結 果 29 一、 LsGRP1C之生體外抑菌能力分析 30 二、 LsGRP1C之植體上抑菌能力分析 30 三、 LsGRP1C對哺乳類紅血球之作用 32 四、 LsGRP1C對細菌細胞之作用 32 五、 以酵母菌表現LsGRP1C之分子選殖及蛋白質產物分析 33 柒、討 論 34 捌、參考文獻 42 玖、圖表集 56 表一、LsGRP1C生體外抑制細菌活性 56 表二、LsGRP1C生體外抑制真菌活性 57 表三、LsGRP1C對細菌之50%抑制濃度 59 表四、LsGRP1C對真菌之50%抑制濃度 60 表五、LsGRP1C對哺乳類紅血球毒性測試 61 表六、酵母菌表現系統引子對序列 62 圖一、LsGRP1胺基酸序列 63 圖二、LsGRP1C抑制甘藍黑斑病之病徵比較 64 圖三、LsGRP1C抑制甘藍黑斑病之病斑直徑比較 65 圖四、不同處理時間對LsGRP1C抑制甘藍黑斑病之影響 66 圖五、處理LsGRP1C對甘藍黑斑病菌感染甘藍葉之影響 67 圖六、甘藍葉上LsGRP1C對黑斑病菌孢子發芽之影響 68 圖七、草莓花灰黴病罹病等級 69 圖八、不同濃度LsGRP1C對草莓花灰黴病病徵發展之影響 70 圖九、不同濃度LsGRP1C對草莓花及幼果灰黴病病徵發展之影響 71 圖十、LsGRP1C前處理對灰黴病菌感染草莓花之影響 72 圖十一、LsGRP1C前處理與後處理對灰黴病菌感染草莓之影響 73 圖十二、LsGRP1C後處理時間對灰黴病菌感染草莓花之影響 74 圖十三、蘭花灰黴病罹病等級 75 圖十四、不同濃度LsGRP1C對蘭花灰黴病病徵發展之影響 76 圖十五、LsGRP1C抑制番茄細菌性斑點病之病徵比較 77 圖十六、LsGRP1C對Pseudomonas syringae細胞外型之影響(高菌量) 78 圖十七、LsGRP1C對Pseudomonas syringae細胞外型之影響(低菌量) 79 圖十八、LsGRP1C對Xanthomonas campestris pv. vesicatoria細胞外型之影響(低菌量) (I) 80 圖十九、LsGRP1C對Xanthomonas campestris pv. vesicatoria細胞外型改變之影響(低菌量) (II) 81 圖二十、LsGRP1C對Xanthomonas campestris pv. campestris 細胞外型之影響(高菌量) ………………………………………………………………………..82 圖二十一、LsGRP1C處理不同時間對Xanthomonas campestris pv. campestris 細胞外型之影響(高菌量) 83 圖二十二、LsGRP1C對Pseudomonas syringae細胞膜之透化 之作用 84 圖二十三、LsGRP1C對Xanthomonas campestris pv. vesicatoria細胞膜之透化作用 ………………………………………………………………………..85 圖二十四、LsGRP1C對Xanthomonas campestris pv. campestris細胞膜之透化作用 ………………………………………………………………………..86 圖二十五、利用Pichia pastoris表現LsGRP1C之建構流程圖 87 圖二十六、利用西方點漬法確認酵母菌表現LsGRP1C蛋白質產物 88 圖二十七、LsGRP1C與pepR胺基酸序列比較 89 拾、附 錄 90 一、質體pPICZB示意圖 91 二、pPICZB酵素切位示意圖 92 | |
| dc.language.iso | zh-TW | |
| dc.title | 百合防禦相關蛋白LsGRP1之胜肽片段抗菌活性與機制探討 | zh_TW |
| dc.title | Study of antimicrobial activity and mechanism of Lilium
defense-related protein LsGRP1-derived peptide | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭安秀,許元勳,吳蕙芬 | |
| dc.subject.keyword | 抗菌胜?LsGRP1 C端胜?抗菌分析, | zh_TW |
| dc.subject.keyword | Antimicrobial peptide,C-terminal peptide of LsGRP1,antimicrobial assay, | en |
| dc.relation.page | 92 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-11-18 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 植物病理與微生物學研究所 | zh_TW |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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