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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡懷楨(Huai-Jen Tsai) | |
| dc.contributor.author | Ting-Lieh Hsieh | en |
| dc.contributor.author | 謝庭列 | zh_TW |
| dc.date.accessioned | 2021-06-07T18:15:11Z | - |
| dc.date.copyright | 2012-04-24 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-03-09 | |
| dc.identifier.citation | Arnold, K., Bordoli, L., Kopp, J. and Schwede, T. (2006). The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics, 22, 195-201.
Chang, S.H., Lee, B.C., Chen, Y.D., Lee, Y.C. and Tsai, H.J. (2011) Development of transgenic zooplankton Artemia as a bioreactor to produce exogenous protein. Transgenic research, 20, 1099-1111. Chang, T.L. (2005). Dual role of α-defensin-1 in anti-HIV-1 innate immunity. Journal of Clinical Investigation, 115, 765-773. Chen, X., Zhu, F., Cao, Y. and Qiao, S. (2009). Novel Expression Vector for Secretion of Cecropin AD in Bacillus subtilis with Enhanced Antimicrobial Activity. Antimicrobial Agents and Chemotherapy, 53, 3683-3689. Chong, D.K. and Langridge, W.H. (2000) Expression of full-length bioactive antimicrobial human lactoferrin in potato plants. Transgenic research, 9, 71-78. Cole, A.M., Weis, P. and Diamond, G. (1997). Isolation and characterization of pleurocidin, an antimicrobial peptide in the skin secretions of winter flounder. The Journal of biological chemistry, 272, 12008-12013. Costa, C., Solanes, G., Visa, J. and Bosch, F. (1998). Transgenic rabbits overexpressing growth hormone develop acromegaly and diabetes mellitus. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 12,1455-1460. de Luis, R., Lavilla, M., Sanchez, L., Calvo, M. and Perez, M.D. (2010). Pepsin degradation of Cry1A(b). protein purified from genetically modified maize (Zea mays). Journal of agricultural and food chemistry, 58, 2548-2553. Devlin, R.H., Biagi, C.A., Yesaki, T.Y., Smailus, D.E. and Byatt, J.C. (2001). Growth of domesticated transgenic fish. Nature, 409, 781-782. Devlin, R.H., D'Andrade, M., Uh, M. and Biagi, C.A. (2004). Population effects of growth hormone transgenic coho salmon depend on food availability and genotype by environment interactions. Proceedings of the National Academy of Sciences of the United States of America, 101, 9303-9308. Devlin, R.H., Sakhrani, D., Tymchuk, W.E., Rise, M.L. and Goh, B. (2009). Domestication and growth hormone transgenesis cause similar changes in gene expression in coho salmon (Oncorhynchus kisutch). Proceedings of the National Academy of Sciences of the United States of America, 106, 3047-3052. Friedrich, C.L., Rozek, A., Patrzykat, A. and Hancock, R.E. (2001). Structure and mechanism of action of an indolicidin peptide derivative with improved activity against gram-positive bacteria. The Journal of biological chemistry, 276, 24015-24022. Gomez-Gil, B., Herrera-Vega, M.A., Abreu-Grobois, F.A. and Roque, A. (1998). Bioencapsulation of two different vibrio species in nauplii of the brine shrimp (Artemia franciscana). Applied and environmental microbiology, 64, 2318-2322. Haag, C.R., Riek, M., Hottinger, J.W., Pajunen, V.I. and Ebert, D. (2005). Genetic diversity and genetic differentiation in Daphnia metapopulations with subpopulations of known age. Genetics, 170, 1809-1820. Hao, Y.Q., Zhou, X.D., Xiao, X.R., Lu, J.J., Zhang, F.C., Hu, T., Wu, H.K. and Chen, X.M. (2005). Effects of cecropin-XJ on growth and adherence of oral cariogenic bacteria in vitro. Chinese medical journal, 118, 155-160. Haug, B.E., Strom, M.B. and Svendsen, J.S. (2007). The medicinal chemistry of short lactoferricin-based antibacterial peptides. Current medicinal chemistry, 14, 1-18. Hultmark, D., Engstrom, A., Bennich, H., Kapur, R. and Boman, H.G. (1982). Insect immunity: isolation and structure of cecropin D and four minor antibacterial components from Cecropia pupae. European journal of biochemistry / FEBS, 127, 207-217. Hwang, G., Muller, F., Rahman, M.A., Williams, D.W., Murdock, P.J., Pasi, K.J., Goldspink, G., Farahmand, H. and Maclean, N. (2004). Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos. Mar Biotechnol (NY), 6, 485-492. Kavanagh, K. and Dowd, S. (2004). Histatins: antimicrobial peptides with therapeutic potential. The Journal of pharmacy and pharmacology, 56, 285-289. Kroll, K.L. and Amaya, E. (1996). Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. Development, 122, 3173-3183. Ladokhin, A.S., Selsted, M.E. and White, S.H. (1999). CD spectra of indolicidin antimicrobial peptides suggest turns, not polyproline helix. Biochemistry, 38, 12313-12319. Laurienzo, P. (2010). Marine polysaccharides in pharmaceutical applications: an overview. Marine drugs, 8, 2435-2465. Li, S.S. and Tsai, H.J. (2000) Transfer of foreign gene to giant freshwater prawn (Macrobrachium rosenbergii) by spermatophore-microinjection. Molecular reproduction and development, 56, 149-154. Lin, C.-C., Lin, J.H.-Y., Chen, M.-S. and Yang, H.-L. (2007). An oral nervous necrosis virus vaccine that induces protective immunity in larvae of grouper (Epinephelus coioides). Aquaculture, 268, 265-273. Lin, C.Y., Yang, P.H., Kao, C.L., Huang, H.I. and Tsai, H.J. (2010). Transgenic zebrafish eggs containing bactericidal peptide is a novel food supplement enhancing resistance to pathogenic infection of fish. Fish and shellfish immunology, 28, 419-427. Liu, Y.L., Zhao, Y., Dai, Z.M., Chen, H.M. and Yang, W.J. (2009). Formation of diapause cyst shell in brine shrimp, Artemia parthenogenetica, and its resistance role in environmental stresses. The Journal of biological chemistry, 284, 16931-16938. Lu, J.K., Fu, B.H., Wu, J.L. and Chen, T.T. (2002). Production of transgenic silver sea bream (Sparus sarba) by different gene transfer methods. Mar Biotechnol (NY)., 4, 328-337. Marcos, J.F., Beachy, R.N., Houghten, R.A., Blondelle, S.E. and Perez-Paya, E. (1995). Inhibition of a plant virus infection by analogs of melittin. Proceedings of the National Academy of Sciences of the United States of America, 92, 12466-12469. Moreno-Garrido, I. (2008). Microalgae immobilization: current techniques and uses. Bioresource technology, 99, 3949-3964. Moriyama, S., Oda, M., Yamazaki, T., Yamaguchi, K., Amiya, N., Takahashi, A., Amano, M., Goto, T., Nozaki, M., Meguro, H. and Kawauchi, H. (2008). Gene structure and functional characterization of growth hormone in dogfish, Squalus acanthias. Zoological science, 25, 604-613. Munari, M., Matozzo, V. and Marin, M.G. (2011). Combined effects of temperature and salinity on functional responses of haemocytes and survival in air of the clam Ruditapes philippinarum. Fish and shellfish immunology, 30, 1024-1030. Ortega, M.A., Diaz-Guerra, M. and Sastre, L. (1996). Actin gene structure in two Artemia species, A. franciscana and A. parthenogenetica. Journal of molecular evolution, 43, 224-235. Papadopoulos, A.I., Lazaridou, E., Mauridou, G. and Touraki, M. (2004). Glutathione S-transferase in the branchiopod Artemia salina. Marine Biology, 144, 295-301. Parraguez, M., Gajardo, G. and Beardmore, J.A. (2009). The New World Artemia species A. franciscana and A. persimilis are highly differentiated for chromosome size and heterochromatin content. Hereditas, 146, 93-103. Rahman, M.A., Mak, R., Ayad, H., Smith, A. and Maclean, N. (1998). Expression of a novel piscine growth hormone gene results in growth enhancement in transgenic tilapia (Oreochromis niloticus).. Transgenic research, 7, 357-369. Renfer, E., Amon-Hassenzahl, A., Steinmetz, P.R. and Technau, U. (2010). A muscle-specific transgenic reporter line of the sea anemone, Nematostella vectensis. Proceedings of the National Academy of Sciences of the United States of America, 107, 104-108. Sakamoto, T. and McCormick, S.D. (2006). Prolactin and growth hormone in fish osmoregulation. General and comparative endocrinology, 147, 24-30. Schibli, D.J., Hwang, P.M. and Vogel, H.J. (1999). The structure of the antimicrobial active center of lactoferricin B bound to sodium dodecyl sulfate micelles. FEBS letters, 446, 213-217. Thériault, I. and Pernet, F. (2007). Lipid nutrition and settlement behaviour in American lobster Homarus americanus. Aquatic Biology, 1, 121-133. Tsai, H.J., Lin, K.L., Kuo, J.C. and Chen, S.W. (1995). Highly efficient expression of fish growth hormone by Escherichia coli cells. Applied and environmental microbiology, 61, 4116-4119. Varo, I., Serrano, R., Navarro, J.C., Lopez, F.J. and Amat, F. (1998). Acute lethal toxicity of the organophosphorus pesticide chlorpyrifos to different species and strains of Artemia. Bulletin of environmental contamination and toxicology, 61, 778-785. Wachinger, M., Kleinschmidt, A., Winder, D., von Pechmann, N., Ludvigsen, A., Neumann, M., Holle, R., Salmons, B., Erfle, V. and Brack-Werner, R. (1998). Antimicrobial peptides melittin and cecropin inhibit replication of human immunodeficiency virus 1 by suppressing viral gene expression. The Journal of general virology, 79 ( Pt 4), 731-740. Wang, J.Q., Hou, L., Yi, N., Zhang, R.F., Zou, X.Y., Xiao, Q. and Guo, R. (2012). Molecular cloning and its expression of trachealess gene (As-trh) during development in brine shrimp, Artemia sinica. Molecular biology reports, 39, 1659-1665. Wang, W., Smith, D.K. and Chen, H.M. (1999). The effect of pH on the structure, binding and model membrane lysis by cecropin B and analogs. Biochimica et biophysica acta, 1473, 418-430. Yada, T. (2007). Growth hormone and fish immune system. General and comparative endocrinology, 152, 353-358. Yamamoto, Y., Kabeya, N., Takeuchi, Y., Higuchi, K., Yatabe, T., Tsunemoto, K., Yazawa, R., Kawamura, T. and Yoshizaki, G. (2011). Establishment of a stable transgenic strain in a pelagic egg spawning marine teleost, Nibe croaker Nibea mitsukurii. Aquaculture, 313, 42-49. Yen, C.C., Shen, C.J., Hsu, W.H., Chang, Y.H., Lin, H.T., Chen, H.L. and Chen, C.M. (2011). Lactoferrin: an iron-binding antimicrobial protein against Escherichia coli infection. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 24, 585-594. Zhang, Y., Arends, J.B., Van de Wiele, T. and Boon, N. (2011). Bioreactor technology in marine microbiology: from design to future application. Biotechnology advances, 29, 312-321. Zweytick, D., Deutsch, G., Andra, J., Blondelle, S.E., Vollmer, E., Jerala, R. and Lohner, K. (2011). Studies on lactoferricin-derived Escherichia coli membrane-active peptides reveal differences in the mechanism of N-acylated versus nonacylated peptides. The Journal of biological chemistry, 286, 21266-21276. Zweytick, D., Tumer, S., Blondelle, S.E. and Lohner, K. (2008). Membrane curvature stress and antibacterial activity of lactoferricin derivatives. Biochemical and biophysical research communications, 369, 395-400. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16443 | - |
| dc.description.abstract | 本研究欲採用甲殼類豐年蝦作為生物反應器。於豐年蝦體中製造魚類的生長激素 (ypGH)及牛的乳鐵蛋白，以提供魚類抗病及促進成長用途。首先，先構築可以表現外來基因的表現質體。透過電穿孔方式轉殖至豐年蝦中，利用綠螢光篩選出100隻G0親代，並與野生種配對，之後利用PCR及螢光篩選出帶有綠螢光的YL6及YL8兩個品系 (strain)的F1子代，育成F2子代，並將F2個體進行自交後，產出F3子代。利用PCR檢測這些F3個體，可偵測到1.3 k的外來基因片段。抽取YL6及YL8兩品系F3子代蛋白質，以tGFP抗體都可偵測到50 kDa的外來重組蛋白質。若以ELISA偵測無節幼蟲帶有的外來重組蛋白質含量，每隻YL6的為0.0011 μg及每隻YL8的為0.0004 μg。另外使用pepsin進行對YL6轉殖豐年蝦蛋白質進行處理，以tGFP抗體可偵測到被pepsin截切後的27 kDa tGFP蛋白質，而以ypGH抗體可以偵測到21 kDa ypGH蛋白質，表示外來重組蛋白確實存在。在透過外加pepsin切下會產生抗菌活性，經抑菌環實驗結果得知YL6每一隻F3轉殖豐年蝦在agar plate顯示出其殺大腸桿菌 (Escherichia coli)的效力。總結，本研究得到可以產製外來蛋白質之穩定遺傳品系豐年蝦，將來可進一步於in vivo進行生物活性試驗，並可應用於水產養殖及醫療等用途。 | zh_TW |
| dc.description.abstract | In this study, we use Artemia sp. as a bioreactor to produce recombinant proteins through whole body. We constructed an expression plasmid and introduced into Artemia by electroporation to generate the transgenic Artemia. We selected 100 GFP-positive Artemia as G0 transgenic founders, and crossed with wild-type individually. In total, two G0 lines which produced GFP-positive F1 offspring were generated. Among F1 offspring, we crossed GFP- positive F1 with wild-type individually to generate F2, and thereof F3 generations of Artemia. Genomic DNAs were extracted from the G0, F1, F2 and F3 individuals and checked with PCR detection, a 1.3 k PCR-product was amplified. Two stable transgenic Artemia lines were screened, named YL6 and YL8. Moreover, a recombinant protein with molecular masses of 50 kDa from transgenic Artemia was positive hybridization with tGFP antiserum. The concentration of recombinant proteins produced by one homozygotic nauplii of YL6 and YL8 were 0.0011 μg and 0.0004 μg, respectively, by ELISA. In addition, in agar-well diffusion assay, the bactericidal functional domain was able to release from fusion protein after it was digested with pepsin, suggesting that the bactericidal efficacy against Escherichia coli from one transgenic Artemia YL6 was positive. In conclusion, this study showed that we successfully generated stable transgenic Artemia lines which may be potentially applied for aquaculture and therapeutic treatment. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T18:15:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 | en |
| dc.description.tableofcontents | 目 錄
中文摘要…………………………………………………………… 1 英文摘要…………………………………………………………… 2 文獻回顧…………………………………………………………….3 前言………………………………………………………………….9 實驗材料與方法……………………………………………………..11 結果…………………………………………………………………..19 討論…………………………………………………………………...24 參考資料……………………………………………………………...28 圖與表…………………………………………………………………34 附錄……………………………………………………………………46 | |
| dc.language.iso | zh-TW | |
| dc.subject | 抗菌蛋白 | zh_TW |
| dc.subject | 生長激素 | zh_TW |
| dc.subject | 豐年蝦 | zh_TW |
| dc.subject | Artemia | en |
| dc.subject | Growth hormone | en |
| dc.subject | Antimicrobial peptide | en |
| dc.title | 基因轉殖豐年蝦表現外源重組蛋白 | zh_TW |
| dc.title | Transgenic Artemia as a Bioreactor to Produce Recombinant Proteins | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蕭世民(Shyh-Min Tom Hsiao),韓玉山(Yu-San Han) | |
| dc.subject.keyword | 豐年蝦,抗菌蛋白,生長激素, | zh_TW |
| dc.subject.keyword | Artemia,Antimicrobial peptide,Growth hormone, | en |
| dc.relation.page | 47 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2012-03-13 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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