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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 廖秀娟 | |
| dc.contributor.author | An-Chieh Kao | en |
| dc.contributor.author | 高安潔 | zh_TW |
| dc.date.accessioned | 2021-06-07T18:05:01Z | - |
| dc.date.copyright | 2012-08-01 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-27 | |
| dc.identifier.citation | 許翔雁 (2007)。台灣烏腳病地區含砷地下水菌種Bosea sp. str. L7506的分離與分析。國立台灣大學生物環境系統工程學研究所碩士論文。
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Environ Sci Pollut Res Int. DOI: 10.1007/s11356-012-0861-9. Zouboulis, A., and Katsoyiannis, I. (2002). Removal of arsenates from contaminated water by coagulation-direct filtration. Sep Sci Technol 37, 2859-2873. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16206 | - |
| dc.description.abstract | 飲用含砷地下水對人類健康所造成的危害已是全球性的議題。雖然地下水含砷在台灣很早就被發現，但影響砷釋放的因子以及如何降低砷汙染仍需探討。本研究中，我們討論農業行為裡肥料的使用(磷肥、銨肥)對砷釋出的影響，並從砷汙染地區篩選出一株具有三價砷氧化能力的菌株，探討其應用於砷的生物復育之潛力。實驗結果顯示，在厭氧環境下磷肥與銨肥皆會使砷自土壤中釋放到地下水。此外，添加磷肥與銨肥會加速鐵的釋出並改變微生物群相。As7325為自砷汙染地區篩選出來的細菌，屬於Pseudomonas屬，異營菌。As7325最適合生長的溫度為30oC，最適合生長的酸鹼值約為7。As7325對砷具有高抗性，可抵抗1 mM三價砷與高於100 mM五價砷。由砷的氧化實驗中發現，利用現地地下水在好氧環境條件下，As7325可以在一天內將30 μM (~ 2250 μg/L)三價砷全部氧化成五價砷。此外，經冷凍乾燥後的As7325可用來做為生物吸附劑；實驗結果顯示As7325能在六天內百分之百吸附6.67 μM (~ 500 μg/L)或13.33 μM (~ 1000 μg/L)的五價砷。以上結果皆顯示As7325在砷的生物復育上具有很大的應用潛力。 | zh_TW |
| dc.description.abstract | The contamination of groundwater with arsenic is a major concern to public health from local to international. Although arsenic groundwater problems in Taiwan had been noted for several years, the factors affecting arsenic release and technology to significantly reduce arsenic level still remain elucidated. In this study, we investigate the effect of agricultural behavior such as fertilizing (ammonium and phosphate) on arsenic mobilization, and isolate an arsenite-oxidizing bacterium As7325 to examine its potential for arsenic bioremediation. The results showed that under anaerobic condition, both phosphate and ammonium caused arsenic release from sediment to groundwater. In addition, the addition of phosphate and ammonium also enhanced the release of ferrous and changed the microbial communities. Bacterial strain As7325, which was isolated from the arsenic-contaminated site, belongs to the genus of Pseudomonas. Strain As7325 is a heterotroph. The optimal temperature for growth was 30oC and the pH was about 7. Strain As7325 could resist high concentration of arsenic with minimum inhibitory concentration at 1 mM for arsenite and over 100 mM for arsenate. Strain As7325 could completely oxidize 30 μM (~ 2250 μg/L) arsenite using in situ groundwater within 1 day under aerobic condition. In addition, lyophilized cell pellet of strain As7325 was used as a biosorbent and could adsorb 6.67 μM (~ 500 μg/L) and 13.33 μM (~ 1000 μg/L) arsenate near 100% at 6-day period. The results showed that strain As7325 has the potential for bioremediation of arsenic. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-07T18:05:01Z (GMT). No. of bitstreams: 1 ntu-101-R99622002-1.pdf: 1223926 bytes, checksum: b05880122c1c2a9b354050d13ca3ac07 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 誌謝 I
中文摘要 II ABSTRACT III TABLE OF CONTENTS V LIST OF FIGURES VII LIST OF TABLES VIII LIST OF ABBREVIATIONS IX CHAPTER 1 INTRODUCTION 1 1.1 Motivation of study 1 1.2 Arsenic in the environment 2 1.3 Arsenic microbial mechanisms 5 1.3.1 Detoxification and resistance system 6 1.3.2 Arsenite-oxidizing microbes 7 1.3.3 Arsenate-reducing microbes 8 1.4 Arsenic bioremediation technology 9 1.5 Purpose of study 11 CHAPTER 2 MATERIALS & METHODS 14 2.1 Sampling site description 14 2.2 Chemicals 14 2.3 Medium compositions 14 2.4 Phosphate and ammonium enrichment assay 18 2.5 Denaturing gradient gel electrophoresis assay 18 2.5.1 PCR for denaturing gradient gel electrophoresis 19 2.5.2 Denaturing gradient gel electrophoresis conditions 20 2.6 Isolation of arsenite-oxidizing bacterial strain As7325 21 2.7 Growth conditions for strain As7325 21 2.8 Substrate test for strain As7325 22 2.9 Minimum inhibitory concentration test for As7325 22 2.10 PCR amplification of 16S rRNA 23 2.11 PCR amplification of aoxB 23 2.12 Phylogenetic analysis 24 2.13 Arsenite transformation assay 24 2.14 Preparation of lyophilized cell pellet 25 2.15 Arsenate biosorption assay 25 CHAPTER 3 RESULTS 26 3.1 Effects of fertilizers on arsenic release 26 3.2 Denaturing gradient gel electrophoresis analysis of microbial diversity 27 3.3 Characterization of the arsenite-oxidizing strain As7325 31 3.3.1 General description of bacterial strain As7325 31 3.3.2 Sequence analysis of strain As7325 31 3.3.3 Substrate test for growth of strain As7325 32 3.3.4 Minimum inhibitory concentration test for strain As7325 39 3.4 Arsenite oxidation by strain As7325 39 3.5 Arsenite-oxidizing marker gene detection (aoxB) 40 3.6 Arsenate biosorption using strain As7325 40 CHAPTER 4 DISCUSSION 48 4.1 The effects of fertilizer utilization on arsenic mobilization 48 4.2 Microbial diversity upon supplemented with fertilizers 49 4.3 Arsenite-oxidizing bacterial strain As7325 from arsenic-contaminated area 50 4.4 Arsenate adsorption by bacterial strain As7325 53 CHAPTER 5 SUGGESTED FUTURE DIRECTION 57 REFERENCES 58 APPENDIXES 70 | |
| dc.language.iso | en | |
| dc.title | 臺灣烏腳病疫區含砷地下水釋出因子及其生物復育 | zh_TW |
| dc.title | The Release Factors and Bioremediation for Arsenic-contaminated Groundwater in
Blackfoot Disease Endemic Region in Taiwan | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 沈偉強,陳昭瑩,王珮玲 | |
| dc.subject.keyword | 砷,銨,磷酸鹽,三價砷氧化菌,地下水,生物復育, | zh_TW |
| dc.subject.keyword | arsenic,ammonium,phosphate,arsenite-oxidizing bacteria,groundwater,bioremediation, | en |
| dc.relation.page | 72 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2012-07-27 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 生物環境系統工程學研究所 | zh_TW |
| 顯示於系所單位： | 生物環境系統工程學系 | |
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