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標題: | 新穎水相合成可分解彈性體之生醫應用研究 Novel biodegradable elastomers from waterborne processes for biomedical applications |
作者: | Chun-Yi Lu 呂俊逸 |
指導教授: | 徐善慧(Shan-hui Hsu) |
關鍵字: | 生醫彈性體,水性聚氨酯,生物可降解性,微相分離,生物相容性,電紡纖維,多孔支架, biomedical elastomer,waterborne polyurethane,biodegradable,microphase separation,biocompatibility,elestrospun fibers,porous scaffolds, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | 本研究藉由改變水性聚胺酯彈性體之軟鏈段種類,以聚己內酯二元醇分別混合聚己二酸乙二醇丁二醇酯二元醇、L型聚乳酸二元醇及DL型聚乳酸二元醇,合成出具有生物可降解性之水性聚胺酯(WBDPU),由此可改變其機械性質及微相分離程度,並可調控降解速率。由體外及體內降解測試結果得知,軟鏈段隨者搭配之聚己二酸己二醇酯二元醇、L型聚乳酸二元醇或DL型聚乳酸二元醇含量增加而有加速降解之趨勢。WBDPU較Pellethane具有較佳之內皮細胞貼附與生長、低免疫反應,並可向上調控eNOS基因表現。WBDPU較Pellethane及PLA具有較低血小板吸附與活化。由大鼠皮下植入實驗之纖維囊厚度得知,WBDPU較PLA具低外來物反應。生物可降解聚胺酯也可加工製備成具有良好內皮細胞相容性之電紡纖維或多孔薄膜,及具有良好間葉幹細胞相容性之多孔支架。因此未來可運用在心血管之生醫材料上,更進一步可應用在軟骨、硬骨方面等組織工程應用上。 Waterborne biodegradable polyurethane (WBDPU) ionomers were synthesized in this study. The soft segments employed the combination of two macrodiols, which were polycaprolactone diol (PCL diol) and a choice from polyethylene butylene adipate diol (PEBA diol) or polylactic acid diol (PLA diol, L-form or D,L-form). The mechanical properties, microphase separation, and degradation rate could be modulated by the ratios between PCL diol and the other macrodiol. The degradation rate of WBDPU was positively correlated with the type and ratio of macrodiols both in vitro and in vivo. WBDPU had better endothelial cell (EC) attachment and proliferation with lower immune response than Pellethane. The gene expression of endothelial nitric oxicde synthase was up-regulated by WBDPU. WBPDU showed lower platelet adhesion and activation than PLA and Pellethane. WBDPU implanted in subcutaneous rats showed lower capsule thickness than PLA, indicating the smaller foreign body reaction. WBDPU could also be conveniently processed into electrospun fibers as well as two-dimensional (2D) porous membranes with adequate EC attachment and three-dimensional (3D) scaffolds with proper mesenchymal stem cell seeding efficiency. The WBDPU synthesized in this study were novel biomedical elastomers that may find applications in the repair and regeneration of cardiovascular and other soft tissues. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15963 |
全文授權: | 未授權 |
顯示於系所單位: | 高分子科學與工程學研究所 |
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ntu-101-1.pdf 目前未授權公開取用 | 10.44 MB | Adobe PDF |
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