請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15841
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝學真(Hsyue-Jen Hsieh) | |
dc.contributor.author | Pin-Wen Chen | en |
dc.contributor.author | 陳品彣 | zh_TW |
dc.date.accessioned | 2021-06-07T17:53:22Z | - |
dc.date.copyright | 2012-08-27 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-17 | |
dc.identifier.citation | 1.Tathe A, Ghodke M, Nikalje AP, A brief review: biomaterials and their application. International Journal of Pharmacy and Pharmaceutical Sciences, 2010. 2(4): p. 19-23.
2.Honarkar H, Barikani M, Applications of biopolymers I: chitosan. Monatshefte für Chemie Chemical Monthly 2009. 140(12): p. 1403-1420. 3.陳播暉, 幾丁聚醣/果膠複合材料之製備與特性探討, 國立臺灣大學化學工程研究所碩士論文. 2004. 4.Qian T, Liping J, Jingwei Z, En Z, Yanyan W, Choosing and application of wound dressings: a literature review. Journal of Nursing Science, 2010. 25(4). 5.Chvapil M, Considerations on manufacturing principles of a synthetic burn dressing: a review. J Biomed Mater Res, 1982. 16(3): p. 245-63. 6.Stamatialis DF, Papenburg BJ, Girones M, Saiful S, Bettahalli SNM, Schmitmeier S, Wessling M, Medical applications of membranes: Drug delivery, artificial organs and tissue engineering. Journal of Membrane Science, 2008. 308(1-2): p. 1-34. 7.許學全, Chitosan對已被覆生醫陶瓷Ti6Al4V植入用合金之影響,中興大學材料工程學系所. 2009. 8.Kumar MNVR, A review of chitin and chitosan applications. Reactive & Functional Polymers, 2000. 46(1): p. 1-27. 9.Shirakura M, Tanimoto K, Eguchi H, Miyauchi M, Nakamura H, Hiyama K, Tanimoto K, Tanaka E, Takata T, Tanne K, Activation of the hypoxia-inducible factor-1 in overloaded temporomandibular joint, and induction of osteoclastogenesis. Biochem Biophys Res Commun, 2010. 393(4): p. 800-805. 10.Klokkevold PR, Subar P, Fukayama H, Bertolami CN, Effect of Chitosan on Lingual Hemostasis in Rabbits with Platelet Dysfunction Induced by Epoprostenol. Journal of Oral and Maxillofacial Surgery, 1992. 50(1): p. 41-45. 11.Muzzarelli RAA, Zucchini C, Ilari P, Pugnaloni A, Belmonte MM, Biagini G, Castaldini C, Osteoconductive Properties of Methylpyrrolidinone Chitosan in an Animal-Model. Biomaterials, 1993. 14(12): p. 925-929. 12.Muzzarelli RAA, Chitin. 1st ed1977, Oxford ; New York: Pergamon Press. xv, 309 p. 13.Khor E, Lim LY, Implantable applications of chitin and chitosan. Biomaterials, 2003. 24(13): p. 2339-2349. 14.曾彥博, 幾丁聚醣-果膠複合材料微粒之製備、特性分析與應用探討, 國立臺灣大學化學工程學研究所碩士論文. 2010. 15.Wu YN, Yang Z, Hui JHP, Ouyang HW, Lee EH, Cartilaginous ECM component-modification of the micro-bead culture system for chondrogenic differentiation of mesenchymal stem cells. Biomaterials, 2007. 28(28): p. 4056-4067. 16.Wang YC, Kao SH, Hsieh HJ, A chemical surface modification of chitosan by glycoconjugates to enhance the cell-biomaterial interaction. Biomacromolecules, 2003. 4(2): p. 224-231. 17.Amiji MM, Surface modification of chitosan membranes by complexation-interpenetration of anionic polysaccharides for improved blood compatibility in hemodialysis. Journal of Biomaterials Science-Polymer Edition, 1996. 8(4): p. 281-298. 18.Tangpasuthadol V, Pongchaisirikul N, Hoven VP, Surface modification of chitosan films. Effects of hydrophobicity on protein adsorption. Carbohydrate Research, 2003. 338(9): p. 937-942. 19.Jeon C HW, Chemical modification of chitosan and equilibrium study for mercury ion removal. Water Research, 2003. 37(19): p. 4770-4780. 20.Muzzarelli RAA, Tanfani F, Scarpini G, Laterza G, The Degree of Acetylation of Chitins by Gas-Chromatography and Infrared-Spectroscopy. Journal of Biochemical and Biophysical Methods, 1980. 2(5): p. 299-306. 21.Vilanova E, Barril J, Carrera V, Biochemical-Properties and Possible Toxicological Significance of Various Forms of Nte. Chemico-Biological Interactions, 1993. 87(1-3): p. 369-381. 22.Dumitriu S, Polysaccharides, Marcel Dekker Inc., New York. 1998. 23.蔡政翰, 以化學修飾法改進幾丁聚醣之溶解度, 國立台灣大學食品科技研究所碩士論文. 1996. 24.Northcote DH, The Cell Walls of Higher Plants - Their Composition, Structure and Growth. Biological Reviews of the Cambridge Philosophical Society, 1958. 33(1): p. 53-102. 25.Walter RH, The Chemistry and technology of pectin. Food science and technology1991, San Diego: Academic Press. xi, 276 p. 26.Nordby MH, Kjoniksen AL, Nystrom B, Roots J, Thermoreversible gelation of aqueous mixtures of pectin and chitosan. Rheology. Biomacromolecules, 2003. 4(2): p. 337-343. 27.Street CA, Anderson DMW, Refinement of Structures Previously Proposed for Gum Arabic and Other Acacia Gum Exudates. Talanta, 1983. 30(11): p. 887-893. 28.Tiss A, Carriere F, Verger R, Effects of gum Arabic on lipase interfacial binding and activity. Analytical Biochemistry, 2001. 294(1): p. 36-43. 29.周奕宏, 以電紡絲法製備幾丁聚醣/硫酸軟骨素/聚乙烯醇複合奈米纖維之研究, 國立臺灣大學化學工程研究所碩士論文. 2011. 30.Fanger PO, Introduction of the olf and the decipol units to quantify air pollution perceived by humans indoors and outdoors. Energy and Buildings, 1988. 12(1): p. 1-6. 31.Lin SY, Chen KS, Liang RC, Design and evaluation of drug-loaded wound dressing having thermo responsive, adhesive, absorptive and easy peeling properties. Biomaterials, 2001. 22(22): p. 2999-3004. 32.黃穎斐, 生醫敷料及人工皮膚. 科學發展, 2004. 308: p. 24-29. 33.Liu HF, Mao JS, Yao KD, Yang GH, Cui L, Cao YL, A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering applications. Journal of Biomaterials Science-Polymer Edition, 2004. 15(1): p. 25-40. 34.Anthony D. Metcalfe MWJF, Bioengineering skin using mechanisms of regeneration and repair. Biomaterials, 2007. 28(34): p. 5100-5113. 35.Sonnemann KJ, Bement WM, Wound repair: toward understanding and integration of single-cell and multicellular wound responses. Annu Rev Cell Dev Biol, 2011. 27: p. 237-63. 36.楊瑞永, 慢性傷口的敷料使用原則. 長庚醫訊, 2008. 29卷 10期: p. 10-12. 37.Lin SY, Chen KS, Run-Chu L, Design and evaluation of drug-loaded wound dressing having thermoresponsive, adhesive, absorptive and easy peeling properties. Biomaterials, 2001. 22(22): p. 2999-3004. 38.Lugao AB, Malmonge SM, Use of radiation in the production of hydrogels. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, 2001. 185: p. 37-42. 39.呂理誌, 材料表面性質影響細胞生長及血清蛋白質吸附之探討, 國立台灣大學化學工程學研究所碩士論文. 1999. 40.Lamke LO, Evaporative water loss from burns under different environmental conditions. Scand J Plast Reconstr Surg, 1971. 5(2): p. 77-81. 41.石政坪, 以UV光聚合Chitosan-PAA-pHEMA水膠應用於創傷敷料之研究, 南台科技大學化學工程與材料工程系碩士論文. 2008. 42.Alves A, Pinho ED, Neves NM, Sousa RA, Reis RL, Processing ulvan into 2D structures: Cross-linked ulvan membranes as new biomaterials for drug delivery applications. Int J Pharm, 2012. 426(1-2): p. 76-81. 43.Tsao CT, Chang CH, Lin YY, Wu MF, Wang JL, Young TH, Han JL, Hsieh KH, Evaluation of chitosan/gamma-poly(glutamic acid) polyelectrolyte complex for wound dressing materials. Carbohydr Polym, 2011. 84(2): p. 812-819. 44.苑乃義, 多孔狀幾丁聚醣基質製備與酵素降解, 國立台灣大學化學工程學研究所碩士論文. 2003. 45.Alani NI, Borazan HN, Interactions of Caffeine and Theophylline with P-Cresol - Uv Studies. Journal of Pharmaceutical Sciences, 1978. 67(10): p. 1381-1384. 46.Alobeidi FA, Borazan HN, Interactions of Nucleic-Acid Bases with Catechol - Uv Studies. Journal of Pharmaceutical Sciences, 1976. 65(6): p. 892-895. 47.Alobeidi FA, Borazan HN, Interactions of Adenine, Thymine, and Uracil with Epinephrine - Uv Studies. Journal of Pharmaceutical Sciences, 1976. 65(7): p. 982-985. 48.林雨利, 酸鹼敏感型幾丁聚醣奈米顆粒之製備與應用, 國立台灣大學化學工程學研究所碩士論文. 2011. 49.Zahedi P, Karami Z, Rezaeian I, Jafari SH, Mahdaviani P, Abdolghaffari AH, Abdollahi M, Preparation and performance evaluation of tetracycline hydrochloride loaded wound dressing mats based on electrospun nanofibrous poly(lactic acid)/poly(ϵ-caprolactone) blends. Journal of Applied Polymer Science, 2012. 124(5): p. 4174-4183. 50.Lamke LO, Liljedahl SO, Evaporative water loss from burns, grafts and donor sites. Scand J Plast Reconstr Surg, 1971. 5(1): p. 17-22. 51.Lamke LO, Nilsson GE, Reithner HL, Evaporative Water-Loss from Burns and Water-Vapor Permeability of Grafts and Artificial Membranes Used in Treatment of Burns. Burns, 1977. 3(3): p. 159-165. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15841 | - |
dc.description.abstract | 本研究主要目的為製備幾丁聚醣與果膠及阿拉伯膠的三成分複合膜材,過程中會針對不同成分比例的混合溶液及製備出的緻密複合膜材,進行一連串基本性質的測試。接著在複合膜材應用層面,會藉由其藥物釋放及透水氣速率(water vapor transmission rate,簡稱WVTR)的測定,以期瞭解此複合材料是否具備成為創傷敷料的潛力。
首先針對不同比例的幾丁聚醣與果膠及阿拉伯膠配製出混合溶液,進行基本的溶液黏度及pH值測試。結果發現於幾丁聚醣中同時加入果膠及阿拉伯膠,可有效地讓溶液黏度自40 cP左右降低至約10 cP,且因為加入的果膠及阿拉伯膠為酸性多醣,所以可使幾丁聚醣溶液pH值由3.91降低至3.1。 之後利用混合溶液製備出緻密膜材,並針對膜材的機械性質、親水性、吸水能力、熱性質以及細胞相容性進行測試。由結果可知添加果膠及阿拉伯膠後,膜材機械強度會由1.49 MPa增強至1.9 MPa,但其延展性會降低,伸長量可由55%降低至28%。此外,因為果膠及阿拉伯膠為親水性材料,因此添加此兩種成分後,會讓膜材的親水性及吸水能力提高,使其接觸角由92.2度降至84度,含水量從膜重的1.3倍增加至2.3倍。而由熱性質測定結果可知緻密膜中的果膠及阿拉伯膠比例提升後,會降低其熱穩定性,熱重分析(TGA)測得的裂解溫度(Td)由純幾丁聚醣膜的311.8 oC下降為281.7 oC;示差掃描熱分析的結晶溫度(Tc)亦由162 oC降為138 oC左右。加入果膠及阿拉伯膠後膜材的崩解14天後剩餘量,在鹼處理過後的膜材部分會從96.5%減少至90.2%,而未經鹼處理的膜材則由75.6%減少至39%。之後在緻密膜上進行細胞相容性的測定,於纖維母細胞的培養成果可顯示,複合膜材不適合纖維母細胞生長,細胞不易貼附於材料上。而間葉幹細胞的培養成果顯示細胞於膜材上雖然生長速度較慢,但在較長時間的培養下,細胞還是能持續增生,藉此可知並非所有細胞都不適合生長在利用幾丁聚醣與果膠及阿拉伯膠製備的膜材上。 而在應用方面,以牛血清蛋白(bovine serum albumin,簡稱BSA)、茶鹼(theophylline)以及四環黴素(tetracycline)作為乘載藥物進行藥物釋放,則可發現在乘載藥物為大分子(BSA)時,藥物置入膜中有延緩釋放的效果,藥物於15小時內皆有持續地釋放,而隨著添加親水性負電材料的比例增加,藥物總釋放量從33.5%提高至64.1%。而若添加藥物為小分子:茶鹼及四環黴素,則非常容易自緻密膜中釋放,最慢亦在5分鐘內即完成藥物的釋出,推測是因茶鹼及四環黴素的分子過小,所以在緻密膜泡入PBS溶液後會因膨潤而造成孔隙變大,使分子可以輕易的從膜材內釋出。而對於水氣透過速率的測試顯示,以乾膜模擬傷口水氣散失結果,複合膜材的水氣透過速率(WVTR)約在2125~2553 g/m2-day符合文獻上提出燒燙傷傷口所需的數值(2000~3000 g/m2-day),但與市售的一般創傷敷料7~8 g/m2-day相比水氣透過速率則超過太多,因此,於複合膜材外加上一層透水氣能力較弱的材料polydimethylsiloxane (PDMS),結果發現的確有降低整體透水氣速率的能力,WVTR 可由2125~2553 g/m2-day降低至172~190 g/m2-day,將可增加膜材的應用廣度。 本實驗成功做出的三成分複合膜材雖無法有效控制四環黴素的釋放速度,但其在機械強度、親水性、吸水性皆有比純幾丁聚醣膜較佳表現。此外,複合膜材的水氣透過速率亦符合燒燙傷需求,外加PDMS層則可使水氣透過率降低至一般創傷敷料需求範圍。由此可知,本研究製作出的三成分複合緻密膜,具有生醫應用上的潛力。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-07T17:53:22Z (GMT). No. of bitstreams: 1 ntu-101-R99524040-1.pdf: 11835756 bytes, checksum: b015523d56466910fc6bb9ad11adf483 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 誌謝 I
摘要 III Abstract V 目錄 VII 圖目錄 XI 表目錄 XV 縮寫與符號說明 XVII 中英對照表 XIX 1. 緒論 1 1.1 研究背景 1 1.2 實驗動機與目的 2 1.3 實驗架構與流程 3 2. 文獻回顧 7 2.1 生醫材料 7 2.1.1 生醫材料的分類 7 2.1.2 複合材料 8 2.2 幾丁聚醣 9 2.3 果膠 12 2.4 阿拉伯膠 16 2.5 皮膚與傷口癒合 18 2.6 生醫敷料 19 2.6.1 生醫敷料種類 19 2.6.2 生醫敷料特性 20 3. 實驗藥品、儀器與方法 21 3.1 實驗材料 21 3.1.1 混合溶液製備 21 3.1.2 複合緻密膜材製備 21 3.1.3 複合緻密膜材性質測試 21 3.1.3.1 崩解性質測定 21 3.1.3.2 生物相容性測定 21 3.1.4 複合緻密膜材相關應用 22 3.1.4.1 藥物釋放特性 22 3.2 實驗儀器 23 3.2.1 一般儀器 23 3.2.2 混合溶液配製 23 3.2.3 複合緻密膜材製備與性質測試 24 3.3 實驗方法 26 3.3.1 混合溶液配製與性質測定 26 3.3.1.1 混合溶液製備 26 3.3.1.2 溶液黏度及pH值 27 3.3.1.3 溶液pH值 27 3.3.2 複合緻密膜材製備與性質測試 27 3.3.2.1 緻密膜材製備 27 3.3.2.2 膜材結構測定 28 3.3.2.3 機械性質測定 28 3.3.2.4 親水性測定 29 3.3.2.5 吸水程度測定 32 3.3.2.6 熱性質測定 33 3.3.2.7 崩解性質測定 34 3.3.2.8 生物相容性測定 35 3.3.3 複合緻密膜材相關應用 37 3.3.3.1 藥物釋放特性 37 3.3.3.2 透水氣性質測定 41 3.3.3.3 PDMS膜材製備 42 3.3.3.4 統計分析 42 4. 結果與討論 43 4.1 混合溶液配製與性質測定 43 4.1.1 溶液黏度 43 4.1.2 溶液pH值 46 4.2 複合緻密膜材製備與性質測定 46 4.2.1 膜材外觀及結構 46 4.2.2 機械性質測定 49 4.2.3 親水性測定 53 4.2.4 吸水程度測定 55 4.2.5 熱性質測定 59 4.2.6 崩解性質測定 64 4.2.7 細胞相容性測定 69 4.3 複合緻密膜材相關應用 81 4.3.1 藥物釋放特性 81 4.3.2 透水氣性質測定 87 5. 結論與未來方向 97 5.1 結論 97 5.2 未來研究方向 100 6. 參考文獻 101 | |
dc.language.iso | zh-TW | |
dc.title | 多成分幾丁聚醣複合敷料之製作及特性測試 | zh_TW |
dc.title | Preparation and Characterization of Multicomponent Chitosan Composite Dressings | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王大銘,謝子陽 | |
dc.subject.keyword | 幾丁聚醣,果膠,阿拉伯膠,緻密膜,敷料,細胞貼附,四環黴素,透水氣速率, | zh_TW |
dc.subject.keyword | chitosan,pectin,Acacia gum,dense film,dressing,cell attachment,tetracycline hydrochloride,water vapor transmission rate, | en |
dc.relation.page | 104 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2012-08-18 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-101-1.pdf 目前未授權公開取用 | 11.56 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。