請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15728
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳克強(Keqiang Wu) | |
dc.contributor.author | Chih-Hua Kuo | en |
dc.contributor.author | 郭致驊 | zh_TW |
dc.date.accessioned | 2021-06-07T17:50:52Z | - |
dc.date.copyright | 2012-11-15 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-11-06 | |
dc.identifier.citation | Abraham, E., Hourton-Cabassa, C., Erdei, L., and Szabados, L. (2010). Methods for determination of proline in plants. Methods in molecular biology 639, 317-331.
Alinsug, M.V., Yu, C.W., and Wu, K. (2009). Phylogenetic analysis, subcellular localization, and expression patterns of RPD3/HDA1 family histone deacetylases in plants. BMC plant biology 9, 37. Antosch, M., Mortensen, S.A., and Grasser, K.D. (2012). Plant proteins containing high mobility group box DNA-binding domains modulate different nuclear processes. Plant physiology 159, 875-883. Benhamed, M., Bertrand, C., Servet, C., and Zhou, D.X. (2006). Arabidopsis GCN5, HD1, and TAF1/HAF2 interact to regulate histone acetylation required for light-responsive gene expression. The Plant cell 18, 2893-2903. Bianchi, M.E., and Beltrame, M. (1998). Flexing DNA: HMG-Box Proteins and Their Partners. American Society of Human Genetics 63, 1573-1577. Chomczynski. (1993). A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532-536. Differentiation, and Development. Cell Growth & Differentiation 13, 95-106. Gonzalez-Guzman, M. (2002). The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde. The Plant Cell Online 14, 1833-1846. Grasser, K.D., T, F., Hansen, Madsen, C.K., Nordland, A.M., Grasser, M., and Merkle, T. (2008). A Novel Family of Plant DNA-Binding Proteins Containing both HMG-Box and AT-Rich Interaction Domains. Biochemistry, 13207–13214. He, Y., and Amasino, R.M. (2005). Role of chromatin modification in flowering-time control. Trends in plant science 10, 30-35. Hiroshi Abe, Kazuko Yamaguchi-Shinozaki, Urao, T., lwasaki, T., Hosokawa, D., and Shinozaki, K. (1997). Role of Arabidopsis MYC and MYB Homologs in Droughtand Abscisic Acid-Regulated Gene Expression. The Plant cell 9, 1859-1868. Imura, Y., Kobayashi, Y., Yamamoto, S., Furutani, M., Tasaka, M., Abe, M., and Araki, T. (2012). CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple target steps in Arabidopsis. Plant & cell physiology 53, 287-303. Imura, Y., Kobayashi, Y., Yamamoto, S., Furutani, M., Tasaka, M., Abe, M., and Araki, T. (2012). CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple target steps in Arabidopsis. Plant & cell physiology 53, 287-303. Kim, J.M., To, T.K., Nishioka, T., and Seki, M. (2010). Chromatin regulation functions in plant abiotic stress responses. Plant, cell & environment 33, 604-611. Kwak, K.J., Kim, J.Y., Kim, Y.O., and Kang, H. (2007). Characterization of transgenic Arabidopsis plants overexpressing high mobility group B proteins under high salinity, drought or cold stress. Plant & cell physiology 48, 221-231. Lu, Q., Tang, X., Tian, G., Wang, F., Liu, K., Nguyen, V., Kohalmi, S.E., Keller, W.A., Tsang, E.W., Harada, J.J., Rothstein, S.J., and Cui, Y. (2010). Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. The Plant journal : for cell and molecular biology 61, 259-270. Michaels. (2004). Vernalization, Competence, and the Epigenetic Memory of Winter. The Plant Cell Online 16, 2553-2559. Moran, E., Wilsker, D., Patsialou, A., and Dallas, P.B. (2002). ARID Proteins: A Diverse Family of DNA Binding Proteins Probst, A.V., Fagard, M., Proux, F., Mourrain, P., Boutet, S., Earley, K., Lawrence, R.J., Pikaard, C.S., Murfett, J., Furner, I., Vaucheret, H., and Mittelsten Scheid, O. (2004). Arabidopsis histone deacetylase HDA6 is required for maintenance of transcriptional gene silencing and determines nuclear organization of rDNA repeats. The Plant cell 16, 1021-1034. Qian, W., Miki, D., Zhang, H., Liu, Y., Zhang, X., Tang, K., Kan, Y., La, H., Li, X., Li, S., Zhu, X., Shi, X., Zhang, K., Pontes, O., Chen, X., Liu, R., Gong, Z., and Zhu, J.K. (2012). A histone acetyltransferase regulates active DNA demethylation in Arabidopsis. Science 336, 1445-1448. Richards, E., Reichardt, M., and Rogers, S. (1994). Preparation of Genomic DNA from Plant Tissue. Current Protocols in Molecular Biology, 2.3.1-2.3.7. Schroeder, J.I., Allen, G.J., Kuchitsu, K., Chu, S.P., and Yoshiyuki Murata. (1999). Arabidopsis abi1-1 and abi2-1 Phosphatase Mutations Reduce Abscisic Acid–Induced Cytoplasmic Calcium Rises in Guard Cells. The Plant cell 11, 1785-1789. Sharma, S., and Verslues, P.E. (2010). Mechanisms independent of abscisic acid (ABA) or proline feedback have a predominant role in transcriptional regulation of proline metabolism during low water potential and stress recovery. Plant, cell & environment 33, 1838-1851. Shinozaki, K., and Yamaguchi-Shinozaki, K. (2007). Gene networks involved in drought stress response and tolerance. Journal of experimental botany 58, 221-227. Verslues, P.E., and Sharma, S. (2010). Proline metabolism and its implications for plant-environment interaction. The Arabidopsis book / American Society of Plant Biologists 8, e0140. Verslues, P.E., and Sharma, S. (2010). Proline metabolism and its implications for plant-environment interaction. The Arabidopsis book / American Society of Plant Biologists 8, e0140. Wilsker, D., Probst, L., Wain, H.M., Maltais, L., Tucker, P.W., and Moran, E. (2005). Nomenclature of the ARID family of DNA-binding proteins. Genomics 86, 242-251. Yu, C.W., Liu, X., Luo, M., Chen, C., Lin, X., Tian, G., Lu, Q., Cui, Y., and Wu, K. (2011). HISTONE DEACETYLASE6 interacts with FLOWERING LOCUS D and regulates flowering in Arabidopsis. Plant physiology 156, 173-184. Zhu, J.K. (2002). Salt and drought stress signal transduction in plants. Annual review of plant biology 53, 247-273. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15728 | - |
dc.description.abstract | ARID 是一個普遍存在於真核生物蛋白質中的DNA結合區,ARID此段區域約由100個胺基酸所組成,並形成螺旋-轉-螺旋的形式結合在DNA的major groove上,結合在DNA AT鹼基對較多的區域。包含ARID區域的蛋白質有非常多的功能,參與在染色質重塑與基因表達,可控制細胞生長與分化。HMG-box區域是由約80個胺基酸所組成,這個區域和DNA也有著高親和力,可藉由和DNA的結合調控下由基因的轉錄。在本研究,我們發現一個阿拉伯芥ARID-HMG1(簡稱AHMG1)蛋白可以和RPD3 類的組蛋白去乙醯酶HDA2及HDA9 相互作用,形成一個複合體以調控下游的基因表達。在ahmg1的knockout突變體比野生型植株有更高的抗旱性及抗鹽性,一些逆境反應及ABA訊息傳導相關的基因如ABI1、ABI2、RD29B等,在ahmg1 突變體中也大量表達。這些結果顯示AHMG1在抗逆境反應重要功能。並參與離層酸的訊息傳遞路徑中。 | zh_TW |
dc.description.abstract | The ARID is an ancient DNA-binding domain that is conserved throughout the evolution of higher eukaryotes. The ARID consensus sequence spans about 100 amino acid residues, and structural studies identify the major groove contact site as a modified helix-turn-helix motif. A subset of ARID family proteins binds DNA specifically at AT-rich sites. ARID-containing proteins exhibit a range of cellular functions, including participation in chromatin remodeling, and regulation of gene expression during cell growth, differentiation, and development. The HMG-box domain was first recognized as duplicated 80-amino-acid regions. All of these proteins share a marked effect on DNA structure and an affinity for distorted DNA structures. In this study, we found that the ARID-containing protein ARID-HMG1 (AHMG1) could interact with RPD3- type HDACs, HDA2 and HDA9 in Arabidopsis, suggesting that AHMG1 may form a complex with HDA2 and HDA9. It was found that ahmg1 mutant plants were more tolerant to salt and drought stresses compared to wild-type. In addition, ABA-responsive genes, ABI1, ABI2, and RD29B, were highly expressed in ahmg mutant plants. These data suggested that AHMG1 may play an important role in plant ABA and salt responsive pathway. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T17:50:52Z (GMT). No. of bitstreams: 1 ntu-101-R99b42020-1.pdf: 2305450 bytes, checksum: b8b2a66bf44ed18d3c5c9bfb2a7ee6b4 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract II Index III List of Abbreviations V List of Figures VII List of Tables IX Introduction 1 Histone modification 1 Chromatin-associated proteins 2 ARID-HMG (AHMG) proteins 2 Abiotic stress responses and signaling pathways in plants 4 Histone modification and abiotic stress responses 6 Materials and Methods 8 Plant materials 8 Quick DNA extraction (Richard et al., 1994) 8 RNA isolation (Chomczynski and Sacchi, 1993) 9 RT-PCR analysis 11 Yeast two hybrid assays 11 Quantitative Real-Time PCR (qPCR) 12 Measurement of germination rates and root lengths 13 High salinity and drought treatment 13 Quantification of ABA contents (Weiler et al., 1985) 14 Stomatal aperture measurements 15 Bimolecular Fluorescence Complementation (BiFC) assay 16 Results 21 Phylogenic analysis and sequence comparison of ARID-HMG proteins 21 Expression of AHMG1 was repressed under ABA and NaCl treatment 21 AHMG1 expression patterns in plant organs 22 Subcellular location of the AHMG1 protein 22 HDA9 and HDA2 interacted with AHMG1 23 Identification of AHMG1 T-DNA knock-out mutants 24 ahmg1 mutants were hypersensitive to ABA and abiotic stress during seed germination 25 ahmg1 mutant plants can tolerance osmotic stress and drought stress. 26 ahmg1 plants had high proline contents 27 Expression of ABA biosynthesis genes 27 ahmg1 mutants were insensitive to salt stress. 28 Expression of salt stress-responsive genes 29 ahmg1 and hda9 mutant plants displayed early flowering phenotype 30 Expression of the flowering-related in ahmg1-1 mutants 30 AHMG1 affeced pollen morphology 30 Discussions 31 AHMG1 proteins might function by interacting with RPD3 type HDACs. 31 AHMG1 proteins are involved in ABA and salt stress in Arabidopsis 31 AHMG1 proteins are involved in osmotic and drought stress. 33 ahmg1 mutants display an early flowering phenotype 35 Figures 37 Tables 70 References 75 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥ARID-HMG1蛋白參與非生物逆境之功能性研究 | zh_TW |
dc.title | Involvement of Arabidopsis ARID-HMG1 in abiotic stress responses | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林讚標(Tsan-Piao Lin),葉開溫(Kai-Wun Yeh),鄭秋萍(Chiu-Ping Cheng),張孟基(Men-Chi Chang) | |
dc.subject.keyword | 阿拉伯芥,ARID-HMG蛋白,離層酸,非生物性逆境,組蛋白去乙醯酶, | zh_TW |
dc.subject.keyword | Arabidopsis,ARID-HMG1,ABA,abiotic stress,histone deacetylases, | en |
dc.relation.page | 79 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2012-11-07 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-101-1.pdf 目前未授權公開取用 | 2.25 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。