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標題: | 阿拉伯芥ARID-HMG1蛋白參與非生物逆境之功能性研究 Involvement of Arabidopsis ARID-HMG1 in abiotic stress responses |
作者: | Chih-Hua Kuo 郭致驊 |
指導教授: | 吳克強(Keqiang Wu) |
關鍵字: | 阿拉伯芥,ARID-HMG蛋白,離層酸,非生物性逆境,組蛋白去乙醯酶, Arabidopsis,ARID-HMG1,ABA,abiotic stress,histone deacetylases, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | ARID 是一個普遍存在於真核生物蛋白質中的DNA結合區,ARID此段區域約由100個胺基酸所組成,並形成螺旋-轉-螺旋的形式結合在DNA的major groove上,結合在DNA AT鹼基對較多的區域。包含ARID區域的蛋白質有非常多的功能,參與在染色質重塑與基因表達,可控制細胞生長與分化。HMG-box區域是由約80個胺基酸所組成,這個區域和DNA也有著高親和力,可藉由和DNA的結合調控下由基因的轉錄。在本研究,我們發現一個阿拉伯芥ARID-HMG1(簡稱AHMG1)蛋白可以和RPD3 類的組蛋白去乙醯酶HDA2及HDA9 相互作用,形成一個複合體以調控下游的基因表達。在ahmg1的knockout突變體比野生型植株有更高的抗旱性及抗鹽性,一些逆境反應及ABA訊息傳導相關的基因如ABI1、ABI2、RD29B等,在ahmg1 突變體中也大量表達。這些結果顯示AHMG1在抗逆境反應重要功能。並參與離層酸的訊息傳遞路徑中。 The ARID is an ancient DNA-binding domain that is conserved throughout the evolution of higher eukaryotes. The ARID consensus sequence spans about 100 amino acid residues, and structural studies identify the major groove contact site as a modified helix-turn-helix motif. A subset of ARID family proteins binds DNA specifically at AT-rich sites. ARID-containing proteins exhibit a range of cellular functions, including participation in chromatin remodeling, and regulation of gene expression during cell growth, differentiation, and development. The HMG-box domain was first recognized as duplicated 80-amino-acid regions. All of these proteins share a marked effect on DNA structure and an affinity for distorted DNA structures. In this study, we found that the ARID-containing protein ARID-HMG1 (AHMG1) could interact with RPD3- type HDACs, HDA2 and HDA9 in Arabidopsis, suggesting that AHMG1 may form a complex with HDA2 and HDA9. It was found that ahmg1 mutant plants were more tolerant to salt and drought stresses compared to wild-type. In addition, ABA-responsive genes, ABI1, ABI2, and RD29B, were highly expressed in ahmg mutant plants. These data suggested that AHMG1 may play an important role in plant ABA and salt responsive pathway. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15728 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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