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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 張耀乾(Yao-Chien Alex Chang) | |
dc.contributor.author | Yu-Chen Lu | en |
dc.contributor.author | 呂毓真 | zh_TW |
dc.date.accessioned | 2021-05-20T21:56:16Z | - |
dc.date.available | 2013-07-27 | |
dc.date.available | 2021-05-20T21:56:16Z | - |
dc.date.copyright | 2010-07-27 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-23 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10759 | - |
dc.description.abstract | 文心蘭(Oncidium)為台灣重要蘭花作物,其光合作用於夏季午間常有降低情況,溫度為主要的環境影響因子,然於生理層面的機制尚未釐清。本研究針對一年內環境因子(如光度、溫度及蒸氣壓差等)與文心蘭光合酵素的關係作進一步探討,以期找出環境因子、光合作用以及Rubisco酵素活性之間的關聯性。
本研究發現,一年中(2009年一月至十二月)文心蘭光合作用日變化可分為三種類型,一月及十二月的日變化為單一波峰,即光合作用在接近中午時達最高值;二月至六月及九月至十一月的日變化為雙峰類型,即光合作用在午間有午休現象(midday depression),其中二月及三月下午的恢復情況良好;七月的光合作用有嚴重的午休現象,在下午沒有恢復。單一波峰類型發生於葉溫低於15 ℃時,光度為此類型光合作用的主要調節因子。三月份時,當葉溫超過33 ℃,蒸氣壓差超過3 kPa時,文心蘭淨光合作用明顯下降。七月份時,當葉溫超過40 ℃,蒸氣壓差超過3 kPa時,文心蘭淨光合作用下降至接近零。中午葉溫上升使蒸氣壓差增加,氣孔導度因而降低,可能因此限制了光合作用的進行。從二月份到七月份,文心蘭一日間淨光合作用開始下降點的葉溫由26.2 ℃提高至42.7 ℃,顯示文心蘭對溫度的反應受到季節改變而有馴化現象。從春季至夏季,文心蘭對熱的耐受性逐漸提高。 短時間的天氣改變即會對文心蘭光合作用產生影響,在夏季晴朗天氣條件下,文心蘭的光合作用午休現象劇烈,下午恢復情形不佳。陰天氣溫下降,有助於減緩午休現象,而光度會影響淨光合作用速率的高低。 測量上述三種光合作用類型的Rubisco的活化程度(% activation)日變化,發現光合作用日變化為單峰類型時,文心蘭Rubisco的活化程度日變化趨勢與光度變化成正比。光合作用日變化為雙峰類型時,文心蘭Rubisco 活化程度一日內變化幅度不大,約維持在60%-70%左右。光合作用出現嚴重的午休現象,在下午沒有回復的情況下,Rubisco活化程度於6:00時約58%,之後隨光度增加而逐漸上升,與淨光合作用速率同於8:00時達光期最高值。10:00之後葉溫上升至37 ℃以上,Rubisco活化程度開始下降,葉溫於12:00時達40 ℃,Rubisco活化程度降至51%,下午沒有恢復。 針對文心蘭Rubisco初始活性(initial activity)及活化狀態受光度影響的研究發現,這兩種特性隨光度之增加而提高。當光度由0 μmol•m-2•s-1提高至600 μmol•m-2•s-1,Rubisco初始活性由12.7 μmol•mg chl-1•hr-1提升至18.2 μmol•mg chl-1•hr-1,Rubisco活化狀態從68%提高至92%,顯示在25 ℃的環境及合宜光度範圍內,提高光度有助於提升Rubisco酵素活性。 在固定的生長條件下的研究發現,栽培於日夜溫35/30 ℃的文心蘭植株,在光度為250 μmol•m-2•s-1,溫度30 ℃以上之生長箱下所測得之淨光合作用速率、氣孔導度及Rubisco活化程度對熱的耐受性較15/13 ℃及25/20 ℃者為佳。栽培於15/13 ℃及25/20 ℃的文心蘭植株,淨光合作用速率及氣孔導度在葉溫達33 ℃時明顯降低,Rubisco活性則於葉溫達39 ℃時有下降現象。 綜上所述,在適當溫度條件下,光度可提高氣孔導度及Rubisco活性,進而提升光合作用速率。高溫逆境下,中午氣溫上升使蒸氣壓差增加,文心蘭氣孔導度因而降低,此為較早出現的生理反應;溫度持續升高可能進一步造成植株體內Rubisco活性下降,形成非氣孔限制因子。而植物在不同生長溫度下,體內光合作用酵素對熱的耐受性有所差異。對文心蘭產業而言,夏天高溫透過噴霧以降低氣溫與蒸氣壓差當可減少高溫對光合作用與生長所帶來的負面影響。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-05-20T21:56:16Z (GMT). No. of bitstreams: 1 ntu-99-R97628116-1.pdf: 783089 bytes, checksum: 9e9b4de2d5369f0499b4ff4956ec2ba3 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 誌謝 I
摘要(Abstract) II Abstract IV 目錄 VII 表目錄 IX 圖目錄 X 前言 (Introduction) 1 前人研究(Literature review) 2 一、文心蘭生育習性 2 二、文心蘭光合作用相關研究 3 三、環境因子對光合作用之影響 4 四、環境因子對Rubisco活性的影響 7 (一)Rubisco活化機制 7 (二)溫度對Rubisco的影響 8 (三)光度對Rubisco的影響 10 五、光合作用測量方法簡介 12 材料與方法(Materials and Methods) 15 一、植物材料 (Plant material) 15 二、試驗場所 (Experimental locations) 15 三、栽培管理方式 (Cultural management) 15 四、試驗設計 (Experimental design) 16 (一) 文心蘭光合作用日變化的週年波動情形 16 (二) 不同光度對文心蘭光合作用及Rubisco酵素活性之影響 16 (三) 不同溫度對文心蘭光合作用及Rubisco酵素活性之影響 16 (四) 文心蘭光合作用與Rubisco酵素活性的日變化 17 (五) 不同天候對文心蘭光合作用之影響 17 五、測量方法 (Measurement Methods) 18 (一) 淨光合作用速率之測量 18 (二) PSⅡ光反應速率測定 18 (三) Rubisco測量方法 19 (四)西方墨點轉印法(Western blot assay)分析蛋白質含量 20 六、統計分析(Statistic Analysis) 22 結果(Results) 24 一、文心蘭光合作用日變化的週年波動情形 24 二、不同光度對文心蘭光合作用及Rubisco酵素活性之影響 31 三、不同溫度對文心蘭光合作用及Rubisco酵素活性之影響 31 四、文心蘭光合作用與Rubisco酵素活性的日變化 32 五、不同天候對文心蘭光合作用之影響 35 討論(Discussion) 88 一、文心蘭光合作用日變化的週年波動情形 88 二、不同光度對文心蘭光合作用及Rubisco酵素活性之影響 91 三、不同溫度對文心蘭光合作用及Rubisco酵素活性之影響 92 四、文心蘭光合作用與Rubisco酵素活性的日變化 93 五、不同天候對文心蘭光合作用之影響 96 結論 98 參考文獻(References) 99 附錄一 SDS-PAGE之溶液及膠體配置 106 附錄二 西方轉漬法之溶液 108 | |
dc.language.iso | zh-TW | |
dc.title | 環境因子對文心蘭光合作用及Rubisco活性之影響 | zh_TW |
dc.title | Effects of Environmental Factors on Photosynthesis and Rubisco Activity of Oncidium Gower Ramsey | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 古森本(Maurice Sun-Ben Ku) | |
dc.contributor.oralexamcommittee | 李金龍,陳駿季,林瑞松 | |
dc.subject.keyword | 文心蘭,午休現象,光合作用, | zh_TW |
dc.subject.keyword | Oncidium,photosynthesis,midday depression,Rubisco, | en |
dc.relation.page | 108 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2010-07-26 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝學研究所 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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