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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳仲瑄(Chung-Hsuan Chen) | |
| dc.contributor.author | Yu-Wen Wang | en |
| dc.contributor.author | 王鈺雯 | zh_TW |
| dc.date.accessioned | 2021-05-20T21:55:29Z | - |
| dc.date.available | 2012-07-29 | |
| dc.date.available | 2021-05-20T21:55:29Z | - |
| dc.date.copyright | 2010-07-29 | |
| dc.date.issued | 2010 | |
| dc.date.submitted | 2010-07-26 | |
| dc.identifier.citation | 1 Venter, A., Nefliu, M. & Cooks, R. G. Ambient desorption ionization mass spectrometry. TrAC Trends in Analytical Chemistry 27, 284-290 (2008).
2 Harris, G. A., Nyadong, L. & Fernandez, F. M. Recent developments in ambient ionization techniques for analytical mass spectrometry. Analyst 133, 1297 - 1301 (2008). 3 Chen, H., Gamez, G. & Zenobi, R. What Can We Learn from Ambient Ionization Techniques? . Journal of the American Society for Mass Spectrometry 20, 1947-1963 (2009). 4 Wiseman, J. M. et al. Desorption electrospray ionization mass spectrometry: Imaging drugs and metabolites in tissues. PNAS 105, 18120–18125 (2008). 5 Takáts, Z., Wiseman, J. M., Gologan, B. & Cooks, R. G. Mass Spectrometry Sampling Under Ambient Conditions with Desorption Electrospray Ionization Science 306, 471 - 473 (2004). 6 Weston, D. J. Ambient ionization mass spectrometry: current understanding of mechanistic theory; analytical performance and application areas. Analyst 135, 661 - 668 (2010). 7 Costa, A. B. & Cooks, R. G. Simulated splashes: Elucidating the mechanism of desorption electrospray ionization mass spectrometry. Chemical Physics Letters 464, 1-8 (2008). 8 Venter, A., Sojka, P. E. & Cooks, R. G. Droplet Dynamics and Ionization Mechanisms in Desorption Electrospray Ionization Mass Spectrometry. Analytical Chemistry 78, 8549-8555 (2006). 9 Costa, A. B. & Cooks, R. G. Simulation of atmospheric transport and droplet–thin film collisions in desorption electrospray ionization. Chem. Commun., 3915–3917 (2007). 10 Ifa, D. R., Wu, C., Ouyang, Z. & Cooks, R. G. Desorption electrospray ionization and other ambient ionization methods: current progress and preview. Analyst 135, 669 - 681 (2010). 11 Pasilis, S. P., Kertesz, V. & Berkel, G. J. V. Surface Scanning Analysis of Planar Arrays of Analytes with Desorption Electrospray Ionization-Mass Spectrometry. Analytical Chemistry 79 5956–5962 (2007). 12 Cody, R. B., Laramée, J. A. & Durst, a. H. D. Versatile New Ion Source for the Analysis of Materials in Open Air under Ambient Conditions. Analytical Chemistry 77 2297-2302 (2005). 13 Shiea, J. et al. Electrospray-assisted laser desorption/ionization mass spectrometry for direct ambient analysis of solids. Rapid Communications in Mass Spectrometry 19, 3701-3704 (2005). 14 Peng, I. X., Shiea, J., Loo, R. R. O. & Loo, J. A. Electrospray-assisted laser desorption/ionization and tandem mass spectrometry of peptides and proteins. Rapid Communications in Mass Spectrometry 21, 2541-2546 (2007). 15 Peng, I. X., Loo, R. R. O., Shiea, J. & Loo, J. A. Reactive-electrospray-assisted laser desorption/ionization for characterization of peptides and proteins. Analytical Chemistry 80, 6995-7003 (2008). 16 Peng, I. X., Loo, R. R. O., Margalith, E., Little, M. W. & Loo, J. A. Electrospray-assisted laser desorption ionization mass spectrometry (ELDI-MS) with an infrared laser for characterizing peptides and proteins. Analyst 135, 767 - 772 (2010). 17 Na, N. et al. Direct Detection of Explosives on Solid Surfaces by Mass Spectrometry with an Ambient Ion Source Based on Dielectric Barrier Discharge. Journal of Mass Spectrometry 42, 1079-1085 (2007). 18 Na, N., Zhao, M., Zhang, S., Yang, C. & Zhang, X. Development of a Dielectric Barrier Discharge Ion Source for Ambient Mass Spectrometry Journal of the American Society for Mass Spectrometry 18, 1859-1862 (2007). 19 Kogelschatz, U., Eliasson, B. & Egli, W. From ozone generators to flat television screens: history and future potential of dielectric-barrier discharges. Pure Applied Chemistry 71, 1819-1828 (1999). 20 Williams, J. P., Patel, V. J., Richard Holland & Scrivens, J. H. The use of recently described ionisation techniques for the rapid analysis of some common drugs and samples of biological origin. Rapid Communications in Mass Spectrometry 20, 1447 - 1456 (2006). 21 Huang, G., Chen, H., Zhang, X., Cooks, R. G. & Ouyang, Z. Rapid screening of anabolic steroids in urine by reactive Desorption Electrospray Ionization. Analytical Chemistry 79, 8327-8332 (2007). 22 Kauppila, T. J. et al. Rapid analysis of metabolites and drugs of abuse from urine samples by desorption electrospray ionization-mass spectrometry. Analyst 132, 868 - 875 (2007). 23 Jackson, A. U., Talaty, N., Cooks, R. G. & Berkel, G. J. V. Salt Tolerance of Desorption Electrospray Ionization (DESI). Journal of the American Society for Mass Spectrometry 18, 2218-2225 (2007). 24 Takáts, Z., Wiseman, J. M. & Cooks, R. G. Ambient mass spectrometry using desorption electrospray ionization (DESI): instrumentation, mechanisms and applications in forensics, chemistry, and biology. Journal of Mass Spectrometry 40, 1261 - 1275 (2005). 25 Kertesz, V. & Berkel, G. J. V. Improved imaging resolution in desorption electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry 22, 2639 - 2644 (2008). 26 Shin, Y.-S., Drolet, B., Mayer, R., Dolence, K. & Basile, F. Desorption Electrospray Ionization-Mass Spectrometry of Proteins. Analytical Chemistry 79 3514–3518 (2007). 27 Hoffmann, E. d. & Stroobant, V. Mass Spectrometry: Principles and Applications. 232 (John Wiley& Sons, Ltd, 2007). 28 Myung, S. et al. Coupling desorption electrospray ionization with ion mobility/mass spectrometry for analysis of protein structure: evidence for desorption of folded and denatured States. JOURNAL OF PHYSICAL CHEMISTRY B 110, 5045-5051 (2006). 29 Nemes, P. & Vertes, A. Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry. Analytical Chemistry 79, 8098-8106 (2007). 30 Takáts, Z., Wiseman, J. M., Gologan, B. & Cooks, R. G. Electrosonic Spray Ionization. A Gentle Technique for Generating Folded Proteins and Protein Complexes in the Gas Phase and for Studying Ion-Molecule Reactions at Atmospheric Pressure. Analytical Chemistry 76, 4050-4058 (2004). 31 Wu, C., Ifa, D. R., Manicke, N. E. & Cooks, R. G. Rapid, Direct Analysis of Cholesterol by Charge Labeling in Reactive Desorption Electrospray Ionization. Analytical Chemistry 81, 7618-7624 (2009). | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10750 | - |
| dc.description.abstract | 大氣壓力游離法這幾年在質譜學領域逐漸的受到重視，因為具有不需樣品前處理的優點。大氣壓力游離法可以直接將大氣中的固體與液體用於質譜分析。在此論文中、我們發展了電極輔助噴灑游離法（EADESI）、它可以直接用於生物樣品的快速分析。EADESI是一種新型的大氣壓力游離法，可以檢測樣本在導體或非導體的樣品盤上。該儀器由三個主要部分：三維移動的樣品盤，高電壓電極，ESI噴霧源。ESI噴霧源以45 °輕靠在電極旁邊。被霧化的溶劑氣體被電極極化後噴灑至樣本上，然後分析物離子化並隨著氣流被引入質譜儀中進行分析。我們的研究結果表明，EADESI的空間解析度可以達到25μm。 EADESI可以對不同的生物樣本有廣泛的線性分析範圍、所以是一個可靠而嶄新的生物樣品分析方法。 | zh_TW |
| dc.description.abstract | Ambient ionization mass spectrometry has recently played an important role in Biological Mass Spectrometry because pre-treatment of samples is not required. It can be used for direct analysis of solid and liquid samples with a simple mass spectrometer. We developed the novel Electrode-Assisted Desorption Electrospray Ionization (EADESI) method for quick biomedical sample analysis. EADESI is a novel ambient ionization method which can be used as detection of samples on nearly any type of substrate. The instrument consists of three major parts: a three-dimensional movable sample plate with ground potential, a high voltage electrode, and a spray source. The spray source and electrode are positioned at about 45° with respect to each other and directly above the sample plate. Solvent nebulized by gas was used as a spray device to spray onto the sample, and then the desorbed analyte ions were introduced into the mass spectrometer. Our results indicated that EADESI can have space resolution of 25 μm. EADESI also has a wide dynamic range for different types of biomedical compounds. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T21:55:29Z (GMT). No. of bitstreams: 1 ntu-99-R97223213-1.pdf: 5464535 bytes, checksum: baa09cfacabffe514c1318aebcd6bff7 (MD5) Previous issue date: 2010 | en |
| dc.description.tableofcontents | ACKNOWLEDGEMENTS 1
中文摘要 2 ABSTRACT 3 CONTENTS 4 LIST OF TABLES 6 LIST OF FIGURES 7 INTRODUCTION 1 I. AMBIENT IONIZATION MASS SPECTROMETRY 1 II. DESORPTION ELECTROSPRAY IONIZATION 4 III. DIRECT ANALYSIS IN REAL TIME 10 IV. ELECTROSPRAY-ASSISTED LASER DESORPTION IONIZATION 14 V. DIELECTRIC BARRIER DISCHARGE IONIZATION 17 VI. ELECTRODE-ASSISTED DESORPTION ELECTROSPRAY IONIZATION (EADESI) 19 METHOD 20 ELECTROSPRAY IONIZATION (ESI) 20 EADESI 20 SAMPLE PREPARATION 24 RESULTS AND DISCUSSIONS 26 DETECTION OF BIOMOLECULES 26 PARAMETERS OF EADESI 33 SPATIAL RESOLUTION 36 LIMIT OF DETECTION (lod) 39 LINEAR RANGE 41 MUTIPLE CHARGING 44 DETECTION OF LARGE MOLECULES (ALBUMIN) 45 COMPARISON FO AMBIENT IONIZATION METHODS 47 CONCLUSION 49 REFERENCES 51 | |
| dc.language.iso | en | |
| dc.title | 電極輔助噴灑游離法的開發及對生物分子的應用 | zh_TW |
| dc.title | Development of Electrode-Assisted Desorption Electrospray Ionization and Its biomedical Applications | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 98-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王亦生(Yi-Sheng Wang),林俊利(Jung-Lee Lin) | |
| dc.subject.keyword | 大氣壓力游離法,電極輔助噴灑游離法, | zh_TW |
| dc.subject.keyword | Ambient ionization,Electrode-Assisted Desorption Electrospray Ionization, | en |
| dc.relation.page | 55 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2010-07-27 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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