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標題: | 利用親疏水性質的DNA檢測晶片研發 Development of On-chip DNA Sensor Using Wettability |
作者: | Chen-Hao Kan 甘鎮豪 |
指導教授: | 楊鏡堂(Jing-Tang Yang) |
關鍵字: | 雜交感測器,親疏水性,被動式,毛細力, DNA,sensor,hybridization,passive,capillary,wettability, |
出版年 : | 2010 |
學位: | 碩士 |
摘要: | 本文之目的在於研發一種被動式的DNA雜交檢測晶片,使用毛細力作為檢測流體的驅動力,並結合接觸角之逆向梯度的流體閥門概念,提出一種不同於傳統光學式、電化學式、或質量感測式的檢測裝置概念,希望藉由雜交前後DNA表面的親疏水性質變化來達到DNA檢測的目的,並經設計實驗討論其可行性。本文提出之裝置檢測概念為:在一親水的玻璃表面上固定一道經末端修飾的探針DNA形成疏水表面,於表面蓋上PDMS製成之微通道,使含有互補DNA的流體能利用毛細力引入通道中,與疏水表面之DNA雜交之後因DNA本身序列之電負性使表面轉為相對親水,使裝置表面由原本的阻擋流體之逆向梯度變為順向梯度,使流體得以繼續通過通道,以此流通與否的直覺的方法來達成檢測目的。
本研究將設計一系列前期測試,包含微通道中毛細現象的觀察、設計裝置尺寸、實驗逆向梯度對通道中流體之影響、建立自組裝分子與疏水修飾之DNA探針表面之接觸角資料庫與實驗DNA雜交之後表面之親疏水性的變化,歸納結果並討論生化自組裝表面於被動式裝置的應用,以及本文提出之被動式感測器實現之可能。本文使用自組裝分子單層膜、DNA固定技術以及PDMS材料,並結合微機電製程,提供一個不同於傳統的檢測概念,晶片製程也更為簡易,此新概念與製作方式與生化表面性質資料可供後人在設計被動式檢測晶片時做為參考,也希望能啟發更多不同種的檢測概念。 The main objective of this paper is to develop a passive DNA hybridization sensor, which drives the analyte by capillary force and combines the concept of reversed contact angle gradient as a fluid valve. We propose a sensing concept that uses the change of the wettability before and after DNA hybridization, which is different from typical optical, electrochemical, or mass-sensitive sensor. Also, we design a series of preliminary experiments to approach this concept. The device we propose is a glass which immobilizes the hydrophobicity-modified DNA on a stripe region, covers a PDMS micro-channel that can draw the analytic fluids in through capillary force. After hybridizing with complementary DNA, the hydrophobic region will turn to be relatively hydrophilic due to the polarity of DNA molecule, so that the fluids can keep creeping through the micro-channel. Consequently, we can sense the DNA very intuitively with the device. We will observe the capillary effect in the micro-channel, design the geometry of the device, create a reversed gradient surface to test how it affects the fluids in the micro-channel, establish the contact angle database of the SAM and probe DNA surface, and record the change of wettability before and after hybridization. After summing up these results, we will discuss the applicant on passive device through the bio-chemical surface we have tested and the possibility to realize the concept we propose. We use SAM, DNA immobilization technology, and PDMS material, combine MEMS process to provide a new sensing concept. With these simple process and bio-chemical surface data, this paper can be a reference while designing another passive sensor. Meanwhile, we hope this can inspire more sensing concept. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10636 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 機械工程學系 |
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