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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉嚞睿 | zh_TW |
| dc.contributor.advisor | Je-Ruei Liu | en |
| dc.contributor.author | 沈冠妮 | zh_TW |
| dc.contributor.author | Kuan-Ni Shen | en |
| dc.date.accessioned | 2026-08-28T16:33:02Z | - |
| dc.date.available | 2026-08-29 | - |
| dc.date.copyright | 2026-08-28 | - |
| dc.date.issued | 2026 | - |
| dc.date.submitted | 2026-07-15 00:00:00 | - |
| dc.identifier.citation | Flori, L., Galgani, G., Bray, G., Ippolito, C., Segnani, C., Pellegrini, C., Citi, V., Bernardini, N., Martelli, A., & Calderone, V. (2025). Development of an adipocyte differentiation protocol using 3T3-L1 cells for the investigation of the browning process: Identification of the PPAR-γ agonist rosiglitazone as a browning reference drug. Frontiers in Pharmacology, 16, 1546456. https://doi.org/10.3389/fphar.2025.1546456
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/104588 | - |
| dc.description.abstract | 第二型糖尿病 (type 2 diabetes, T2D) 以胰島素阻抗為主要特徵，其中脂肪組織發炎扮演關鍵角色。ELOVL6 為參與脂質代謝之脂肪酸延長酶，可透過調控脂肪酸組成影響代謝穩態。本研究利用 3T3-L1 細胞模型，探討 ELOVL6 抑制 (knockdown, KD) 是否依脂肪細胞分化階段不同而調控發炎相關基因表現。於脂多醣 (lipopolysaccharide, LPS) 刺激下，ELOVL6 KD 在早期脂肪細胞中降低 Tnf 與 Il6 表現，並增加 Ccl2 表現；然而在成熟脂肪細胞中，則觀察到 Tnf 與 Il6 表現有上升趨勢、Ccl2 表現有下降趨勢，且脂質累積未出現 ELOVL6 KD 依賴性之顯著改變，顯示 ELOVL6 對發炎反應的影響可能受到脂肪細胞分化階段所影響。此結果顯示 ELOVL6 對發炎反應的調控可能具有分化階段依賴性(differentiation-stage-dependent)，而非單純地抑制或促進發炎反應。其機制可能與脂肪酸組成改變所導致之脂質重塑有關，進而影響膜相關訊號傳遞與壓力反應途徑。整體而言，本研究顯示，在總脂質累積量未明顯改變的情況下，ELOVL6 KD 仍可能影響發炎相關基因表現，提示脂質組成或脂質重塑可能參與脂肪細胞發炎反應的調控。然而，此機制仍需進一步透過脂質體學分析加以驗證。 | zh_TW |
| dc.description.abstract | Type 2 diabetes (T2D) is characterized by insulin resistance, in which adipose tissue inflammation plays a key role. ELOVL6, a fatty acid elongase, regulates lipid composition and has been implicated in metabolic homeostasis, although its role in inflammation remains unclear. This study used a 3T3-L1 adipocyte differentiation model to examine whether ELOVL6 knockdown (KD) differentially affects inflammation-associated gene expression at different stages of adipocyte differentiation. Following lipopolysaccharide (LPS) stimulation, ELOVL6 KD reduced Tnf and Il6 expression but increased Ccl2 expression in early-stage adipocytes. In contrast, in mature adipocytes, ELOVL6 KD showed a tendency toward increased Tnf and Il6 expression and reduced Ccl2 expression, while not significantly affecting lipid accumulation. These findings suggest that ELOVL6 knockdown may influence inflammatory gene expression in a differentiation-stage-dependent manner. Since lipid accumulation was not significantly altered, the observed changes in inflammatory gene expression may be related to lipid remodeling rather than total lipid quantity; however, this mechanism requires further validation by lipidomic analysis. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2026-08-28T16:33:02Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2026-08-28T16:33:02Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iii Chapter 1 Introduction 1 1.1 Global Burden of Obesity and Chronic Inflammation 1 1.2 Lipid-Mediated Inflammatory Signaling in Adipocytes 2 1.2.1 TLR4-Mediated Inflammatory Signaling 2 1.2.2 Membrane Structure and Lipid Raft Regulation 4 1.2.3 In Vitro Modeling Using 3T3-L1 Cells 5 1.3 ELOVL6 in Metabolic Regulation and Insulin Resistance 6 1.3.1 The ELOVL Family and Fatty Acid Elongation 6 1.3.2 ELOVL6 and Inflammatory Signaling 8 1.4 Research Gap and Hypothesis 10 1.5 Lipid Remodeling and Membrane-Dependent Signaling 12 1.6 Research Objective and Approach 14 Chapter 2 Materials and Methods 16 2.1 Cell Culture and Adipocyte Differentiation 16 2.2 siRNA Transfection and LPS Treatment 17 2.3 RNA Extraction and Quantitative Real-Time PCR 19 2.4 Oil Red O Staining and Quantification 19 2.5 Statistical Analysis 20 Chapter 3 Results 21 3.1 Validation of Elovl6 Knockdown 21 3.2 Effects of ELOVL6 Knockdown on Inflammatory Gene Expression in Early-Stage Adipocytes 21 3.3 Effects of ELOVL6 Knockdown on Inflammatory Gene Expression in Mature Adipocytes 23 3.4 Effects of ELOVL6 Knockdown on Lipid Accumulation 24 Chapter 4 Discussion 27 4.1 Overview of Key Findings 27 4.2 Stage-Dependent Regulation of Inflammatory Responses 27 4.3 Mechanistic Insights: Lipid Remodeling and Signaling Pathways 29 4.4 Implications for Adipocyte Biology and Metabolic Inflammation 31 Chapter 5 Limitations and Future Perspectives 33 Chapter 6 Conclusion and Way Forward 35 6.1 Conclusion and Way Forward 35 6.2 Translational Potential of ELOVL6 in Metabolic Diseases 36 6.3 Regional Perspectives: Context-Specific Approaches in Japan, Taiwan, and France 36 6.4 Implications for Metabolic Health and Future Strategies 37 References 39 Appendix Table 43 | - |
| dc.language.iso | en | - |
| dc.subject | ELOVL6 | - |
| dc.subject | 脂肪細胞分化 | - |
| dc.subject | 發炎訊號傳遞 | - |
| dc.subject | 3T3-L1 細胞 | - |
| dc.subject | 脂多醣 | - |
| dc.subject | 第二型糖尿病 | - |
| dc.subject | ELOVL6 | - |
| dc.subject | adipocyte differentiation | - |
| dc.subject | inflammatory signaling | - |
| dc.subject | 3T3-L1 cells | - |
| dc.subject | lipopolysaccharide | - |
| dc.subject | type 2 diabetes | - |
| dc.title | ELOVL6於3T3-L1脂肪細胞中對脂多醣誘導發炎反應之調控作用 | zh_TW |
| dc.title | ELOVL6 Regulates LPS-Induced Inflammation in 3T3-L1 Adipocytes | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 114-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 饒梓明;楊俊逸 | zh_TW |
| dc.contributor.oralexamcommittee | Tzu-Ming Jao;Jun-Yi Yang | en |
| dc.subject.keyword | ELOVL6; 脂肪細胞分化; 發炎訊號傳遞; 3T3-L1 細胞; 脂多醣; 第二型糖尿病 | zh_TW |
| dc.subject.keyword | ELOVL6; adipocyte differentiation; inflammatory signaling; 3T3-L1 cells; lipopolysaccharide; type 2 diabetes | en |
| dc.relation.page | 43 | - |
| dc.identifier.doi | 10.6342/NTU202601976 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2026-07-15 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 國際三校農業生技與健康醫療碩士學位學程 | - |
| dc.date.embargo-lift | N/A | - |
| 顯示於系所單位： | 國際三校農業生技與健康醫療碩士學位學程 | |
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