請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/104399完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳育騏 | zh_TW |
| dc.contributor.advisor | Yu-Chi Wu | en |
| dc.contributor.author | 郭怡葶 | zh_TW |
| dc.contributor.author | Yi-Ting Guo | en |
| dc.date.accessioned | 2026-08-26T16:20:16Z | - |
| dc.date.available | 2026-08-27 | - |
| dc.date.copyright | 2026-08-26 | - |
| dc.date.issued | 2026 | - |
| dc.date.submitted | 2026-07-28 00:00:00 | - |
| dc.identifier.citation | Allen, H.K., Trachsel, J., Looft, T., Casey, T.A., 2014. Finding alternatives to antibiotics. Annals of the New York Academy of Sciences 1323(1), 91-100.
Anné, J., Economou, A., Bernaerts, K., 2016. Protein secretion in gram-positive bacteria: from multiple pathways to biotechnology. Protein and sugar export and assembly in gram-positive bacteria, 267-308. Awate, S., Mubarka, S., Huber, R.G., 2023. Whole Genomic Characterization of Streptococcus iniae Isolates from Barramundi (Lates calcarifer) and Preliminary Evidence of Cross-Protective Immunization. Vaccines (Basel) 11(9). Bacou, E., Walk, C., Rider, S., Litta, G., Perez-Calvo, E., 2021. Dietary Oxidative Distress: A Review of Nutritional Challenges as Models for Poultry, Swine and Fish. Antioxidants (Basel) 10(4). Belkaid, Y., Hand, T.W., 2014. Role of the microbiota in immunity and inflammation. Cell 157(1), 121-141. Bergamo, A., Sava, G., 2024. Lysozyme: A Natural Product with Multiple and Useful Antiviral Properties. Molecules 29(3). Bhar, S., Zhao, G., Bartel, J.D., Sterchele, H., Del Mazo, A., Emerson, L.E., Edelmann, M.J., Jones, M.K., 2022. Bacterial extracellular vesicles control murine norovirus infection through modulation of antiviral immune responses. Frontiers in Immunology Volume 13 - 2022. Biller, J.D., Takahashi, L.S., 2018. Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity. Anais da Academia Brasileira de Ciências 90, 3403-3414. Bjørgen, H., Koppang, E.O., 2022. Anatomy of teleost fish immune structures and organs. Principles of fish immunology: from cells and molecules to host protection, 1-30. Bogdan, C., 2015. Nitric oxide synthase in innate and adaptive immunity: an update. Trends in immunology 36(3), 161-178. Bose, S., Aggarwal, S., Singh, D.V., Acharya, N., 2020. Extracellular vesicles: An emerging platform in gram-positive bacteria. Microbial Cell 7(12), 312. Briaud, P., Carroll, R.K., 2020. Extracellular vesicle biogenesis and functions in gram-positive bacteria. Infection and immunity 88(12), 10.1128/iai. 00433-00420. Brown, L., Wolf, J.M., Prados-Rosales, R., Casadevall, A., 2015. Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi. Nature Reviews Microbiology 13(10), 620-630. Busatto, S., Vilanilam, G., Ticer, T., Lin, W.L., Dickson, D.W., Shapiro, S., Bergese, P., Wolfram, J., 2018. Tangential Flow Filtration for Highly Efficient Concentration of Extracellular Vesicles from Large Volumes of Fluid. Cells 7(12). Cao, Y., Lin, H., 2021. Characterization and function of membrane vesicles in Gram-positive bacteria. Applied microbiology and biotechnology 105(5), 1795-1801. Cavalli, G., Colafrancesco, S., Emmi, G., Imazio, M., Lopalco, G., Maggio, M.C., Sota, J., Dinarello, C.A., 2021. Interleukin 1α: a comprehensive review on the role of IL-1α in the pathogenesis and treatment of autoimmune and inflammatory diseases. Autoimmunity reviews 20(3), 102763. Cecil, J.D., O’Brien-Simpson, N.M., Lenzo, J.C., Holden, J.A., Singleton, W., Perez-Gonzalez, A., Mansell, A., Reynolds, E.C., 2017. Outer membrane vesicles prime and activate macrophage inflammasomes and cytokine secretion in vitro and in vivo. Frontiers in immunology 8, 1017. Chaichana, N., Boonsan, J., Dechathai, T., Suwannasin, S., Singkhamanan, K., Wonglapsuwan, M., Pomwised, R., Surachat, K., 2025. Comparative genomic analysis of the Pediococcus genus reveals functional diversity for fermentation and probiotic applications. Computational and Structural Biotechnology Journal. Chang, C.-K., Lin, Z.-L., Kai, Y.-H., Peng, M.-Y., Wu, Y.-C., 2025. Dietary supplementation with Pediococcus pentosaceus promotes resistance to betanodavirus and ranavirus in giant groupers. Aquaculture 599, 742090. Chao, C.B., Yang, S.C., Tsai, H.Y., Chen, C.Y., Lin, C.S., Huang, H.T., 2002. A nested PCR for the detection of grouper iridovirus in Taiwan (TGIV) in cultured hybrid grouper, giant seaperch, and largemouth bass. Journal of Aquatic Animal Health 14(2), 104-113. Chen, T.-t., Tan, L.-r., Hu, N., Dong, Z.-q., Hu, Z.-g., Jiang, Y.-m., Chen, P., Pan, M.-h., Lu, C., 2018. C-lysozyme contributes to antiviral immunity in Bombyx mori against nucleopolyhedrovirus infection. Journal of insect physiology 108, 54-60. Chen, T., Xie, M.-Y., Sun, J.-J., Ye, R.-S., Cheng, X., Sun, R.-P., Wei, L.-M., Li, M., Lin, D.-L., Jiang, Q.-Y., Xi, Q.-Y., Zhang, Y.-L., 2016. Porcine milk-derived exosomes promote proliferation of intestinal epithelial cells. Scientific Reports 6(1), 33862. Chinchar, V.G., Hick, P., Ince, I.A., Jancovich, J.K., Marschang, R., Qin, Q., Subramaniam, K., Waltzek, T.B., Whittington, R., Williams, T., 2017. ICTV virus taxonomy profile: Iridoviridae. Journal of General Virology 98(5), 890-891. Chomová, N., Ratvaj, M., Mudroňová, D., Popelka, P., Mareš, J., Palíková, M., Faldyna, M., 2025. Feed enriched by autochthonous probiotic bacteria and its effect on growth and immune response under different feeding regimes and immune response after Aeromonas salmonicida infection in rainbow trout (Oncorhynchus mykiss). Aquaculture International 33(4), 267. Davis, T., 1985. Seasonal changes in gonad maturity, and abundance of larvae and early juveniles of barramundi, Lates calcarifer (Bloch), in Van Diemen Gulf and the Gulf of Carpentaria. Australian journal of marine and freshwater research 36(2), 177-190. Deng, Z., Rong, Y., Teng, Y., Zhuang, X., Samykutty, A., Mu, J., Zhang, L., Cao, P., Yan, J., Miller, D., 2017. Exosomes miR-126a released from MDSC induced by DOX treatment promotes lung metastasis. Oncogene 36(5), 639-651. Du, S., Guan, Y., Xie, A., Yan, Z., Gao, S., Li, W., Rao, L., Chen, X., Chen, T., 2023. Extracellular vesicles: a rising star for therapeutics and drug delivery. Journal of Nanobiotechnology 21(1), 231. Elvington, M., Liszewski, M.K., Atkinson, J.P., 2016. Evolution of the complement system: from defense of the single cell to guardian of the intravascular space. Immunological reviews 274(1), 9-15. Erdei, A., Sándor, N., Mácsik‐Valent, B., Lukácsi, S., Kremlitzka, M., Bajtay, Z., 2016. The versatile functions of complement C3‐derived ligands. Immunological reviews 274(1), 127-140. Froese, R., 2005. FishBase. world wide web electronic publication. fishbase. org. Fu, G.H., Bai, Z.Y., Xia, J.H., Liu, F., Liu, P., Yue, G.H., 2013. Analysis of two lysozyme genes and antimicrobial functions of their recombinant proteins in Asian seabass. PLoS One 8(11), e79743. Gao, Q., Zhou, W., Nurxat, N., Xu, K., Xu, Y., Li, W., Chen, T., Hu, C., Dong, L., Liu, Q., Li, M., 2025. Dynamic Profiling of Lipoteichoic Acid (LTA) and/or Lipopolysaccharide (LPS) Positive Extracellular Vesicles in Plasma as Diagnostic and Prognostic Biomarkers for Bacterial Infection. Advanced Science (Weinh) 12(44), e06613. Gao, Y., Tian, T., 2023. mTOR Signaling Pathway and Gut Microbiota in Various Disorders: Mechanisms and Potential Drugs in Pharmacotherapy. International Journal of Molecular Sciences 24(14), 11811. Glencross, B., 2006. The nutritional management of barramundi, Lates calcarifer–a review. Aquaculture Nutrition 12(4), 291-309. Gomez, D., Sunyer, J.O., Salinas, I., 2013. The mucosal immune system of fish: the evolution of tolerating commensals while fighting pathogens. Fish Shellfish Immunol 35(6), 1729-1739. Gong, L., He, H., Li, D., Cao, L., Khan, T.A., Li, Y., Pan, L., Yan, L., Ding, X., Sun, Y., 2019. A new isolate of Pediococcus pentosaceus (SL001) with antibacterial activity against fish pathogens and potency in facilitating the immunity and growth performance of grass carps. Frontiers in Microbiology 10, 1384. Gong, Y., Jin, L., Duan, L., Xiao, J., Li, Y., Wang, H., Wang, H., Lin, W., Zhang, Y., Gan, X., Pang, S., Qiu, Y., Lai, W., Zheng, L., Li, H., 2025. Extracellular Vesicles Derived From Streptococcus anginosus Aggravate Lupus Nephritis by Triggering TLR2-MyD88-NF-κB Signalling in NK Cells. J Extracell Vesicles 14(7), e70134. Grenier, D., Mayrand, D., 1987. Functional characterization of extracellular vesicles produced by Bacteroides gingivalis. Infection and Immunity 55(1), 111-117. Guo, C., He, J., Xu, X., Weng, S., He, J., 2025. Megalocytivirus: A Review of Epidemiology, Pathogenicity, Immune Evasion, and Prevention Strategies. Reviews in Aquaculture 17(3), e70025. Gurung, S., Perocheau, D., Touramanidou, L., Baruteau, J., 2021. The exosome journey: from biogenesis to uptake and intracellular signalling. Cell Communication and Signaling 19(1), 47. Harikrishnan, R., Balasundaram, C., Heo, M.-S., 2010. Molecular studies, disease status and prophylactic measures in grouper aquaculture: Economic importance, diseases and immunology. Aquaculture 309(1), 1-14. He, J., Chen, N.N., Li, Z.M., Wang, Y.Y., Weng, S.P., Guo, C.J., He, J.G., 2021. Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein. Int J Mol Sci 22(19). Hoseinifar, S.H., Yousefi, S., Van Doan, H., Ashouri, G., Gioacchini, G., Maradonna, F., Carnevali, O., 2020. Oxidative Stress and Antioxidant Defense in Fish: The Implications of Probiotic, Prebiotic, and Synbiotics. Reviews in Fisheries Science & Aquaculture 29(2), 198-217. Hu, R., Lin, H., Wang, M., Zhao, Y., Liu, H., Min, Y., Yang, X., Gao, Y., Yang, M., 2021. Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharide-induced inflammatory responses in broilers. Journal of Animal Science and Biotechnology 12(1), 25. Huang, D., Taha, M.S., Nocera, A.L., Workman, A.D., Amiji, M.M., Bleier, B.S., 2023. Cold exposure impairs extracellular vesicle swarm–mediated nasal antiviral immunity. Journal of Allergy and Clinical Immunology 151(2), 509-525.e508. Huang, J.-B., Wu, Y.-C., Chi, S.-C., 2014. Dietary supplementation of Pediococcus pentosaceus enhances innate immunity, physiological health and resistance to Vibrio anguillarum in orange-spotted grouper (Epinephelus coioides). Fish & shellfish immunology 39(2), 196-205. Jayathilaka, E.T., Dias, M.K.H.M., Nikapitiya, C., De Zoysa, M., 2024. Immunomodulatory responses of extracellular vesicles released by gram-positive fish pathogen Streptococcus parauberis. Fish & Shellfish Immunology 148, 109508. Jeong, D., Kim, M.J., Park, Y., Chung, J., Kweon, H.-S., Kang, N.-G., Hwang, S.J., Youn, S.H., Hwang, B.K., Kim, D., 2022. Visualizing extracellular vesicle biogenesis in gram-positive bacteria using super-resolution microscopy. BMC biology 20(1), 270. Jeong, M.-A., Jeong, Y.-J., Kim, K.-I., 2021. Complete Genome Sequences and Pathogenicity Analysis of Two Red Sea Bream Iridoviruses Isolated from Cultured Fish in Korea. Fishes 6(4), 82. Jeyaram, A., Jay, S.M., 2017. Preservation and Storage Stability of Extracellular Vesicles for Therapeutic Applications. Aaps j 20(1), 1. Jiang, S., Cai, L., Lv, L., Li, L., 2021. Pediococcus pentosaceus, a future additive or probiotic candidate. Microbial Cell Factories 20(1), 45. Jones, J.E., Le Sage, V., Lakdawala, S.S., 2021. Viral and host heterogeneity and their effects on the viral life cycle. Nature Reviews Microbiology 19(4), 272-282. Kang, J.-H., Kim, J.-H., Gim, J.-A., Lee, M.-Y., 2025. iNOS in Macrophage Polarization: Pharmacological and Regulatory Insights. International Journal of Molecular Sciences 26(24), 12056. Kerddee, P., Dinh-Hung, N., Dong, H.T., Hirono, I., Soontara, C., Areechon, N., Srisapoome, P., Kayansamruaj, P., 2021. Molecular evidence for homologous strains of infectious spleen and kidney necrosis virus (ISKNV) genotype I infecting inland freshwater cultured Asian sea bass (Lates calcarifer) in Thailand. Arch Virol 166(11), 3061-3074. Khatua, A.K., Taylor, H.E., Hildreth, J.E., Popik, W., 2009. Exosomes packaging APOBEC3G confer human immunodeficiency virus resistance to recipient cells. Journal of virology 83(2), 512-521. Kilercioğlu, S., 2021. Fish immune system, mucosal immunity and functions of IL-1β, TNF-α and IL-18 proinflammatory cytokines. Kim, K.H., Kang, G., Woo, W.S., Sohn, M.Y., Son, H.J., Kwon, M.G., Kim, J.O., Park, C.I., 2023. Impact of Red Sea Bream Iridovirus Infection on Rock Bream (Oplegnathus fasciatus) and Other Fish Species: A Study of Horizontal Transmission. Animals (Basel) 13(7). Kim, W., Lee, E.J., Bae, I.-H., Myoung, K., Kim, S.T., Park, P.J., Lee, K.-H., Pham, A.V.Q., Ko, J., Oh, S.H., 2020. Lactobacillus plantarum-derived extracellular vesicles induce anti-inflammatory M2 macrophage polarization in vitro. Journal of extracellular vesicles 9(1), 1793514. King, A.M., Lefkowitz, E., Adams, M.J., Carstens, E.B., 2011. Virus taxonomy: ninth report of the International Committee on Taxonomy of Viruses. Elsevier. Kleinert, H., Schwarz, P.M., Förstermann, U., 2003. Regulation of the expression of inducible nitric oxide synthase. Biological chemistry 384(11), 1343-1364. Krishnan, R., Jeena, K., Kurcheti, P.P., 2021. Nervous necrosis virus induced oxidative imbalance and host associated antioxidant response in Asian seabass brain. Aquaculture 531, 735809. Krishnan, R., Qadiri, S.S.N., Kim, J.-O., Kim, J.-O., Oh, M.-J., 2019. Validation of housekeeping genes as candidate internal references for quantitative expression studies in healthy and nervous necrosis virus-infected seven-band grouper (Hyporthodus septemfasciatus). Fisheries and Aquatic Sciences 22, 1-8. Kurita, J., Nakajima, K., 2012. Megalocytiviruses. Viruses 4(4), 521-538. Magnadóttir, B., 2006. Innate immunity of fish (overview). Fish & shellfish immunology 20(2), 137-151. Małaczewska, J., Kaczorek-Łukowska, E., Wójcik, R., Siwicki, A.K., 2019. Antiviral effects of nisin, lysozyme, lactoferrin and their mixtures against bovine viral diarrhoea virus. BMC veterinary research 15(1), 318. Mashoof, S., Criscitiello, M.F., 2016. Fish immunoglobulins. Biology 5(4), 45. Matic, S., Dia, V.P., 2022. Bovine milk exosomes affected proliferation of macrophages under hypoxia. Current Research in Food Science 5, 2108-2113. Matinha-Cardoso, J., Gonçalves, G., Coutinho, F., Lima, S., Bonneville, L., Serrano, M., Tamagnini, P., Oliva-Teles, A., Couto, A., Serra, C.R., Oliveira, P., 2026. Cyanobacterial Extracellular Vesicles as Protein Carriers: Towards Fish Vaccination. Microbial Biotechnology 19(1), e70294. Mittelbrunn, M., Sánchez-Madrid, F., 2012. Intercellular communication: diverse structures for exchange of genetic information. Nature reviews Molecular cell biology 13(5), 328-335. Mokhtar, D.M., Zaccone, G., Alesci, A., Kuciel, M., Hussein, M.T., Sayed, R.K., 2023. Main components of fish immunity: An overview of the fish immune system. Fishes 8(2), 93. Moore, R., 1979. Natural sex inversion in the giant perch (Lates calcarifer). Australian Journal of Marine and Freshwater Research 30(6), 803-813. Mutoloki, S., Jørgensen, J.B., Evensen, Ø., 2014. The adaptive immune response in fish. Fish vaccination, 104-115. Naeem, P., Baumgartner, A., Ghaderi, N., Sefat, F., Alhawamdeh, M., Heidari, S., Shahzad, F., Swaminathan, K., Akhbari, P., Isreb, M., Anderson, D., Wright, A., Najafzadeh, M., 2023. Anticarcinogenic impact of extracellular vesicles (exosomes) from cord blood stem cells in malignant melanoma: A potential biological treatment. Joural of Cellular and Molecular Medicine 27(2), 222-231. Pamplona, R., Costantini, D., 2011. Molecular and structural antioxidant defenses against oxidative stress in animals. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 301(4), R843-R863. Parida, S., Sahoo, P.K., 2023. Antioxidant Defence in Labeo rohita to biotic and abiotic stress: insight from mRNA expression, molecular characterization and recombinant protein-based ELISA of catalase, glutathione peroxidase, CuZn superoxide dismutase, and glutathione S-transferase. Antioxidants 13(1), 18. Peregrino, E.S., Castañeda-Casimiro, J., Vázquez-Flores, L., Estrada-Parra, S., Wong-Baeza, C., Serafín-López, J., Wong-Baeza, I., 2024. The Role of Bacterial Extracellular Vesicles in the Immune Response to Pathogens, and Therapeutic Opportunities. International Journal of Molecular Science 25(11). Puagsopa, J., Tongviseskul, N., Jaroentomeechai, T., Meksiriporn, B., 2025. Recent Progress in Developing Extracellular Vesicles as Nanovehicles to Deliver Carbohydrate-Based Therapeutics and Vaccines. Vaccines 13(3), 285. Qi, Y., Huang, L., Zeng, Y., Li, W., Zhou, D., Xie, J., Xie, J., Tu, Q., Deng, D., Yin, J., 2021. Pediococcus pentosaceus: screening and application as probiotics in food processing. Frontiers in Microbiology 12, 762467. Raposo, G., Stoorvogel, W., 2013. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol 200(4), 373-383. Ratvaj, M., Maruščáková, I.C., Popelka, P., Fečkaninová, A., Koščová, J., Chomová, N., Mareš, J., Malý, O., Žitňan, R., Faldyna, M., Mudroňová, D., 2023. Feeding-Regime-Dependent Intestinal Response of Rainbow Trout after Administration of a Novel Probiotic Feed. Animals (Basel) 13(12). Sadler, A.J., Williams, B.R.G., 2008. Interferon-inducible antiviral effectors. Nature Reviews Immunology 8(7), 559-568. Sakai, M., Hikima, J.-i., Kono, T., 2021. Fish cytokines: current research and applications. Fisheries Science 87(1), 1-9. Salinas, I., Fernández-Montero, Á., Ding, Y., Sunyer, J.O., 2021. Mucosal immunoglobulins of teleost fish: A decade of advances. Developmental & Comparative Immunology 121, 104079. Sarjoon, M., Amarasinghe, A., Abdul-Careem, M., 2016. Activation of toll-like receptor signaling pathways leading to nitric oxide-mediated antiviral responses. Archives of Virology 161, 2075-2086. Saurabh, S., Sahoo, P.K., 2008. Lysozyme: an important defence molecule of fish innate immune system. Aquaculture Research 39(3), 223-239. Sayed, R.K., Zaccone, G., Capillo, G., Albano, M., Mokhtar, D.M., 2022. Structural and functional aspects of the spleen in molly fish Poecilia sphenops (Valenciennes, 1846): synergistic interactions of stem cells, neurons, and immune cells. Biology 11(5), 779. Simmonds, P., Adriaenssens, E.M., Lefkowitz, E.J., Oksanen, H.M., Siddell, S.G., Zerbini, F.M., Alfenas-Zerbini, P., Aylward, F.O., Dempsey, D.M., Dutilh, B.E., Freitas-Astúa, J., García, M.L., Hendrickson, R.C., Hughes, H.R., Junglen, S., Krupovic, M., Kuhn, J.H., Lambert, A.J., Łobocka, M., Mushegian, A.R., Penzes, J., Muñoz, A.R., Robertson, D.L., Roux, S., Rubino, L., Sabanadzovic, S., Smith, D.B., Suzuki, N., Turner, D., Van Doorslaer, K., Vandamme, A.M., Varsani, A., 2024. Changes to virus taxonomy and the ICTV Statutes ratified by the International Committee on Taxonomy of Viruses (2024). Archives of Virology 169(11), 236. Smith, N.C., Rise, M.L., Christian, S.L., 2019. A comparison of the innate and adaptive immune systems in cartilaginous fish, ray-finned fish, and lobe-finned fish. Frontiers in immunology 10, 2292. Son, V.M., Chang, C.-C., Wu, M.-C., Guu, Y.-K., Chiu, C.-H., Cheng, W., 2009. Dietary administration of the probiotic, Lactobacillus plantarum, enhanced the growth, innate immune responses, and disease resistance of the grouper Epinephelus coioides. Fish & Shellfish Immunology 26(5), 691-698. Sundaram, K., Miller, D.P., Kumar, A., Teng, Y., Sayed, M., Mu, J., Lei, C., Sriwastva, M.K., Zhang, L., Yan, J., 2019. Plant-derived exosomal nanoparticles inhibit pathogenicity of Porphyromonas gingivalis. Iscience 21, 308-327. Tathode, M.S., Bonomo, M.G., Zappavigna, S., Mang, S.M., Bocchetti, M., Camele, I., Caraglia, M., Salzano, G., 2024. Whole-genome analysis suggesting probiotic potential and safety properties of Pediococcus pentosaceus DSPZPP1, a promising LAB strain isolated from traditional fermented sausages of the Basilicata region (Southern Italy). Frontiers in microbiology 15, 1268216. Todorov, S.D., Dioso, C.M., Liong, M.-T., Nero, L.A., Khosravi-Darani, K., Ivanova, I.V., 2023. Beneficial features of pediococcus: from starter cultures and inhibitory activities to probiotic benefits. World Journal of Microbiology and Biotechnology 39(1), 4. Trobridge, G.D., Leong, J.-A.C., 1995. Characterization of a rainbow trout Mx gene. Journal of Interferon & Cytokine Research 15(8), 691-702. Tucker, J.W., Jr., Mackinnon, M.R., Russell, D.J., O'Brien, J.J., Cazzola, E., 1988. Growth of Juvenile Barramundi (Lates calcarifer) on Dry Feeds. The Progressive Fish-Culturist 50(2), 81-85. Valadi, H., Ekström, K., Bossios, A., Sjöstrand, M., Lee, J.J., Lötvall, J.O., 2007. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nature cell biology 9(6), 654-659. Verhelst, J., Hulpiau, P., Saelens, X., 2013. Mx proteins: antiviral gatekeepers that restrain the uninvited. Microbiology and Molecular Biology Reviews 77(4), 551-566. Vicente-Gil, S., Nuñez-Ortiz, N., Morel, E., Serra, C.R., Docando, F., Díaz-Rosales, P., Tafalla, C., 2024. Immunomodulatory properties of Bacillus subtilis extracellular vesicles on rainbow trout intestinal cells and splenic leukocytes. Front Immunol 15, 1394501. Vinh, N.T., Dong, H.T., Lan, N.G.T., Sangsuriya, P., Salin, K.R., Chatchaiphan, S., Senapin, S., 2023. Immunological response of 35 and 42 days old Asian seabass (Lates calcarifer, Bloch 1790) fry following immersion immunization with Streptococcus iniae heat-killed vaccine. Fish & Shellfish Immunology 138, 108802. Wang, I.-H., Burckhardt, C.J., Yakimovich, A., Greber, U.F., 2018a. Imaging, tracking and computational analyses of virus entry and egress with the cytoskeleton. Viruses 10(4), 166. Wang, X., Thompson, C.D., Weidenmaier, C., Lee, J.C., 2018b. Release of Staphylococcus aureus extracellular vesicles and their application as a vaccine platform. Nat Commun 9(1), 1379. Wang, Y., Zhao, D., Hu, J., Bao, Z., Wang, M., 2022. Proteomic analysis of exosomes in pacific oyster Crassostrea gigas during bacterial stimulation. Fish & Shellfish Immunology 127, 1024-1032. Weisburg, W.G., Barns, S.M., Pelletier, D.A., Lane, D.J., 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173(2), 697-703. Wu, K.K.-l., Xu, X., Wu, M., Li, X., Hoque, M., Li, G.H.Y., Lian, Q., Long, K., Zhou, T., Piao, H., 2024. MDM2 induces pro-inflammatory and glycolytic responses in M1 macrophages by integrating iNOS-nitric oxide and HIF-1α pathways in mice. Nature communications 15(1), 8624. Wu, Y.-C., Kai, Y.-H., Chi, S.-C., 2013. Persistently betanodavirus-infected barramundi (Lates calcarifer) exhibit resistances to red sea bream iridovirus infection. Developmental & Comparative Immunology 41(4), 666-674. Wu, Y.-C., Lu, Y.-F., Chi, S.-C., 2010. Anti-viral mechanism of barramundi Mx against betanodavirus involves the inhibition of viral RNA synthesis through the interference of RdRp. Fish & shellfish immunology 28(3), 467-475. Wu, Y.-C., Tsai, C.-H., Chi, S.-C., 2021. Ammonium produced from Shewanella strain 0409 contributes to antiviral activity against Betanodavirus. Aquaculture 533, 736131. Xing, C.F., Hu, H.H., Huang, J.B., Fang, H.C., Kai, Y.H., Wu, Y.C., Chi, S.C., 2013. Diet supplementation of Pediococcus pentosaceus in cobia (Rachycentron canadum) enhances growth rate, respiratory burst and resistance against photobacteriosis. Fish Shellfish Immunol 35(4), 1122-1128. Xu, J., Duan, X., Yang, J., Beeching, J.R., Zhang, P., 2013. Enhanced reactive oxygen species scavenging by overproduction of superoxide dismutase and catalase delays postharvest physiological deterioration of cassava storage roots. Plant Physiol 161(3), 1517-1528. Xu, Y., Xie, C., Liu, Y., Qin, X., Liu, J., 2023. An update on our understanding of Gram-positive bacterial membrane vesicles: discovery, functions, and applications. Frontiers in Cellular and Infection Microbiology 13, 1273813. Yang, T., Yan, W., 2025. Strategies for enhancing the antibacterial efficacy of lysozyme and the resulting outcome. International Journal of Biological Macromolecules 310, 143137. Yang, Y., Song, W., Lin, H., Immune responses of asian seabass lates calcarifer to dietary Glycyrrhiza uralensis. Animals. 2020; 10 (9): 1629. Yu, Y., Wang, Q., Huang, Z., Ding, L., Xu, Z., 2020. Immunoglobulins, mucosal immunity and vaccination in teleost fish. Frontiers in immunology 11, 567941. Zaborowski, M.P., Balaj, L., Breakefield, X.O., Lai, C.P., 2015. Extracellular Vesicles: Composition, Biological Relevance, and Methods of Study. Bioscience 65(8), 783-797. Zarantonello, A., Revel, M., Grunenwald, A., Roumenina, L.T., 2023. C3‐dependent effector functions of complement. Immunological reviews 313(1), 120-138. Zhang, B., Li, S., Ding, J., Guo, J., Ma, Z., Duan, H., 2025. Rho-GTPases subfamily: cellular defectors orchestrating viral infection. Cellular & Molecular Biology Letters 30(1), 55. Zhang, M., Viennois, E., Prasad, M., Zhang, Y., Wang, L., Zhang, Z., Han, M.K., Xiao, B., Xu, C., Srinivasan, S., Merlin, D., 2016. Edible ginger-derived nanoparticles: A novel therapeutic approach for the prevention and treatment of inflammatory bowel disease and colitis-associated cancer. Biomaterials 101, 321-340. Zhao, X., Sun, M., Gong, Y., 2025. Exosome-mediated ACSL4 mRNA delivery suppresses WSSV infection by promoting ferroptosis in mud crab. Aquaculture 598, 742078. Zhu, Z., Duan, C., Li, Y., Huang, C., Weng, S., He, J., Dong, C., 2021. Pathogenicity and histopathology of infectious spleen and kidney necrosis virus genotype II (ISKNV-II) recovering from mass mortality of farmed Asian seabass, Lates calcarifer, in Southern China. Aquaculture 534, 736326. Zidan, A.A., Al-Hawwas, M., Perkins, G.B., Mourad, G.M., Stapledon, C.J.M., Bobrovskaya, L., Zhou, X.-F., Hurtado, P.R., 2021. Characterization of Urine Stem Cell-Derived Extracellular Vesicles Reveals B Cell Stimulating Cargo. International Journal of Molecular Sciences 22(1), 459. 李浚維, 2023. 新型態複合式益生菌對金目鱸成長及免疫反應之影響. 國立臺灣海洋大學水產養殖學系學位論文, 1-64. 栗田潤, 中島員洋, 廣野育生, 青木宙, 1998. Polymerase chain reaction (PCR) amplification of DNA of red sea bream iridovirus (RSIV). 魚病研究 33(1), 17-23. 農業部, 2024. 台灣水產養殖產業市場分析與商機趨勢報告. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/104399 | - |
| dc.description.abstract | 金目鱸 (Lates calcarifer) 為臺灣具重要經濟價值之養殖魚種，在高密度養殖條件下易發生傳染性疾病爆發。其中，嘉鱲虹彩病毒 (Red Sea Bream Iridovirus, RSIV) 為全球養殖及野生魚類族群中造成高死亡率的最嚴重病原之一。胞外囊泡 (Extracellular vesicles, EVs) 為細胞 (包含細菌) 所釋放之具膜結構顆粒，可作為細胞間通訊的媒介，調控多種生理與病理過程。先前研究已證實，植物乳桿菌屬的戊糖片球菌Pediococcus pentosaceus 4012菌株 (LAB4012) 可對魚類病毒具有防治效果。然而,LAB4012所分泌之細胞外囊泡 (EV4012) 是否為其主要抗病毒因子，以及其潛在的抗病毒機制，仍尚未釐清。因此，本研究旨在純化EV4012，並評估其是否具有抵抗RSIV感染的能力。本研究利用切向流過濾技術 (Tangential Flow Filtration, TFF) 自LAB4012培養上清液中純化EV4012，並透過穿透式電子顯微鏡 (Transmission Electron Microscopy, TEM) 與奈米顆粒追蹤分析 (Nanoparticle Tracking Analysis, NTA) 觀察到EV4012具有球形與杯狀之型態，大小約為81 nm，顆粒濃度約為3.49 x 1010 particles/ mL。在穩定性方面，EV4012可儲存於 −80°C長達8個月。此外，體外實驗使用金目鱸腎臟細胞株 (Seabass Kidney cell line, SK) 評估EV4012的安全濃度以及對RSIV之抗病毒活性，結果顯示，稀釋128倍以上的EV4012 接種到SK 細胞24小時不會造成顯著的細胞毒性，並且EV4012在細胞中可以有效中和RSIV。將EV4012作為飼料添加劑，連續餵食金目鱸四週，不會對魚隻造成死亡以及各項組織造成損傷。此外，餵食EV4012能夠提升金目鱸頭腎、脾臟的IL-1β、Mx、C3、IgM、IgT 以及 iNOS等免疫相關基因來提升魚體對RSIV感染之抵抗能力。顯示其在金目鱸養殖中具有作為抵抗RSIV感染之潛在應用價值。 | zh_TW |
| dc.description.abstract | Barramundi (Lates calcarifer) is an economically important aquaculture species in Taiwan. However, under intensive farming conditions, it is highly susceptible to outbreaks of infectious diseases. Among these, Red Sea Bream Iridovirus (RSIV) is one of the most devastating pathogens, causing high mortality in both cultured and wild fish populations worldwide. Extracellular vesicles (EVs) are membrane-bound nanoparticles secreted by cells, including bacteria, and serve as important mediators of intercellular communication by regulating various physiological and pathological processes. Previous studies have demonstrated that Pediococcus pentosaceus strain 4012 (LAB4012), a lactic acid bacterium, exhibits antiviral activity against fish viruses. However, whether the extracellular vesicles secreted by LAB4012 (EV4012) are the major antiviral factor and the underlying mechanisms responsible for their antiviral activity remain unclear. Therefore, this study aimed to isolate and characterize EV4012 and evaluate its antiviral potential against RSIV infection. EV4012 was purified from the culture supernatant of LAB4012 using tangential flow filtration (TFF). Transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) revealed that EV4012 exhibited spherical and cup-shaped morphology with an average particle size of approximately 81 nm and a concentration of 3.49 × 10¹⁰ particles/mL. Stability analysis showed that EV4012 remained stable after storage at −80°C for up to eight months. In vitro, the seabass kidney (SK) cell line was used to evaluate the cytotoxicity and antiviral activity of EV4012. The results showed that EV4012 diluted at least 128-fold exhibited no significant cytotoxicity after 24 h of treatment. Moreover, EV4012 effectively neutralized RSIV infection in SK cells. For the in vivo study, barramundi was fed an EV4012-supplemented diet continuously for four weeks. No mortality or histopathological abnormalities were observed in the experimental fish. Furthermore, dietary supplementation with EV4012 significantly upregulated the expression of immune-related genes, including IL-1β, Mx, C3, IgM, IgT, and iNOS, in the head kidney and spleen, thereby enhancing the host immune response against RSIV infection. These findings demonstrate that EV4012 has considerable potential as a functional feed additive for preventing RSIV infection in barramundi aquaculture. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2026-08-26T16:20:16Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2026-08-26T16:20:16Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 論文口試委員審定書 I
謝辭 II 摘要 III Abstract IV 目次 VI 圖次 VIII 表次 IX 1 文獻回顧 1 1.1 金目鱸 1 1.2 虹彩病毒 1 1.3 魚類的免疫系統 2 1.3.1 先天性免疫 (Innate immunity) 2 1.3.2 後天性免疫 (Adaptive immunity) 3 1.3.3 魚類主要免疫器官 3 1.4 益生菌 4 1.5 細胞外囊泡 (Extracellular vesicles, EVs) 5 2 研究目的 7 3 材料與方法 8 3.1 細菌、細胞株與病毒 8 3.2 胞外囊泡之分離與純化 8 3.3 胞外囊泡之分析 9 3.4 細胞安全劑量試驗 10 3.5 抗病毒活性試驗 10 3.6 餵食EV4012對金目鱸生長表現之影響以及抗RSIV的活性 11 3.7 餵食EV4012對金目鱸調節免疫基因與器官組織切片之影響 12 3.8 RNA的萃取 12 3.9 反轉錄合成 cDNA 13 3.10 即時定量聚合酶連鎖反應 (Quantitative real time PCR) 13 3.11 統計分析 14 4 實驗結果 15 4.1 胞外囊泡的純化平台 15 4.2 不同濃度的EV4012對SK細胞之安全劑量分析 15 4.3 EV4012對SK細胞抗病毒活性之分析 16 4.4 餵食不同濃度EV4012對金目鱸安全性及生長狀況之影響 16 4.5 餵食不同濃度EV4012對金目鱸脾臟與頭腎的免疫基因表現 17 4.6 餵食不同濃度EV4012對金目鱸腸道的免疫基因表現 18 4.7 餵食不同濃度的EV4012對金目鱸各個組織之病理影響 20 4.8 餵食不同濃度的EV4012對金目鱸感染RSIV後存活率之影響 20 5 討論 22 6 結論 30 7 參考文獻 31 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 金目鱸 | - |
| dc.subject | 巨大細胞病毒 | - |
| dc.subject | 戊糖片球菌 | - |
| dc.subject | 細胞外囊泡 | - |
| dc.subject | 飼料添加劑 | - |
| dc.subject | Barramundi | - |
| dc.subject | Megalocytivirus | - |
| dc.subject | Pediococcus pentosaceus | - |
| dc.subject | extracellular vesicles | - |
| dc.subject | feed additive | - |
| dc.title | 戊糖片球菌胞外囊泡對巨大細胞病毒的抗病毒活性及其對金目鱸的免疫調節作用 | zh_TW |
| dc.title | Antiviral activity of extracellular vesicles from Pediococcus pentosaceus against megalocytivirus and its immunomodulatory effects in barramundi | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 114-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 韓玉山;胡鄴方;蓋玉軒 | zh_TW |
| dc.contributor.oralexamcommittee | Yu-San Han;Yeh-Fang Hu;Yu-Hsuan Kai | en |
| dc.subject.keyword | 金目鱸; 巨大細胞病毒; 戊糖片球菌; 細胞外囊泡; 飼料添加劑 | zh_TW |
| dc.subject.keyword | Barramundi; Megalocytivirus; Pediococcus pentosaceus; extracellular vesicles; feed additive | en |
| dc.relation.page | 59 | - |
| dc.identifier.doi | 10.6342/NTU202602667 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2026-07-29 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 漁業科學研究所 | - |
| dc.date.embargo-lift | 2031-07-28 | - |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-114-2.pdf 未授權公開取用 | 3.56 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。