請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/104133完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鄭光成 | zh_TW |
| dc.contributor.advisor | KUAN-CHEN CHENG | en |
| dc.contributor.author | 詹沛青 | zh_TW |
| dc.contributor.author | Pei-Ching Chan | en |
| dc.date.accessioned | 2026-08-24T16:05:46Z | - |
| dc.date.available | 2026-08-25 | - |
| dc.date.copyright | 2026-08-24 | - |
| dc.date.issued | 2026 | - |
| dc.date.submitted | 2026-08-14 12:16:32 | - |
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林欣平 (2016) 細菌性纖維之生產及其於傷口癒創之應用。國立臺灣大學生物科研究所學位論文。臺北，臺灣 林云心 (2024) 利用木質醋酸菌和基因工程之大腸桿菌共培養生合成多孔性細菌性纖維素/紫色桿菌素複合膜作為水中重金屬吸附之研究。國立臺灣大學生物科研究所學位論文。臺北，臺灣 洪翎 (2019) 以幾丁聚醣修飾之泡沫培養基生產多孔性細菌纖維素作為活性包材之應用。國立臺灣大學食品科技研究所學位論文。臺北，臺灣 黃亦尊 (2015) 開發普魯藍抗菌活性包材用於延長冷凍鯛魚片保存期限之研究。國立臺灣大學食品科技研究所學位論文。臺北，臺灣 盛柳娟 (2022) 以泡沫培養基生產多孔性細菌性纖維與其材料特性分析。國立臺灣大學生物科研究所學位論文。臺北，臺灣 陳玠廷 (2014) 利用交聯化改良普魯藍薄膜之物理特性。國立臺灣大學生物科研究所學位論文。臺北，臺灣 顧子嵐 (2024) 利用共培養木質醋酸菌和基因工程大腸桿菌製備多孔性細菌性纖維/黑色素複合膜及其重金屬離子吸附功能之研究。國立臺灣大學生物科研究所學位論文。臺北，臺灣 盧元明 (2024) 順序培養 Komagataeibacter xylinus 和 Lactococcus lactis 以生產抗菌乳鏈菌素多孔性細菌纖維素與其材料特性分析。國立臺灣大學生物科研究所學位論文。臺北，臺灣 Ahmed, M., Saini, P., & Iqbal, U. (2021). Microbial cellulose based films and composites for food packaging: A review. The Annals of the University Dunarea de Jos of Galati. Fascicle VI-Food Technology, 45(1), 178-198. Ahmed, W., Xu, T., Mahmood, M., Núñez-Delgado, A., Ali, S., Shakoor, A., Qaswar, M., Zhao, H., Liu, W., & Li, W. (2022). 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/104133 | - |
| dc.description.abstract | 食品保存與安全已成為全球重視的議題，然而傳統食品包裝多僅具備被動隔離功能，對於延長食品保存期限與抑制品質劣變效果有限。為此，本研究旨在開發一種具抗氧化功能的活性包裝材料，結合泡沫狀細菌性纖維素 (foaming bacterial cellulose, FBC) 與天然類胡蘿蔔素角黃素 (canthaxanthin, CX)，以提升
其保鮮與應用潛力。FBC 係利用含 cremodan 與 xanthan 的泡沫培養基培養Komagataeibacter sucrofermentans 所得，CX 萃取液則透過 Escherichia. coli/pAC-CANTHipi 培養液中進行萃取，並依據培養液體積與萃取溶液體積比 (1 : 1、3 : 1、5 : 1、7 : 1、10 : 1) 區分為不同濃度組別 (CX1、CX3、CX5、CX7與CX10)，進行萃取後用於 FBC 之異位修飾並製得 FBC/CX 膜材。研究結果顯示，FBC 成功製備並順利以 CX 進行異位修飾，修飾後之 FBC/CX 膜呈現濃度依賴性之橘紅色外觀，抗氧化分析亦證實 CX1 至 CX7 萃取液具有良好線性增長之抗氧化能力，並於 FBC/CX10 測得樣品之 Trolox 當量為4.86 ± 0.73 mg/g，自由基清除率達 21.30 ± 1.43 %，遠高於未修飾 FBC (1.10 ± 0.202 mg/g 與 3.30 ± 0.74 %)。細胞實驗結果亦證實所有膜材對 NIH-3T3 小鼠胚胎成纖維細胞皆具良好生物相容性。進一步應用於鱸魚冷藏包裝試驗中， FBC/CX10 展現優異的抑菌與抗氧化效果，在總生菌數分析結果中，FBC/CX10 於第 3 天相較 Blank 組抑菌率達 73.95 %，第 9 與第 12 天則分別達到 76.52 % 與 77.62 %。在脂質氧化指標方面，儲存第 3 天硫代巴比妥酸反應物質變化結果顯示 FBC/CX10 樣品之丙二醛濃度為 0.68 ± 0.05 mg/g，顯著低於未包裝 Blank 組 (1.25 ± 0.49 mg/g) 與 FBC 組 (1.52 ± 0.16 mg/g) ，顯示 FBC/CX10 薄膜於冷藏初期能有效抑制脂質氧化，透過此試驗可知該薄膜可有效抑制前 3 天之總生菌數到達腐敗標準及延緩硫代巴比妥酸反應物質變化指標上升，證實其於活性食品包裝應用具高度潛力。 | zh_TW |
| dc.description.abstract | Food preservation and safety have become globally important issues. However, conventional food packaging typically offers only passive barrier functions and provides limited effectiveness in extending shelf life or preventing quality deterioration. To address this limitation, this study aimed to develop an active packaging material with antioxidant properties by integrating foaming bacterial cellulose (FBC) and a natural carotenoid, canthaxanthin (CX), to enhance preservation performance and application potential. FBC was produced by cultivating Komagataeibacter sucrofermentans in a foamed medium containing cremodan and xanthan, while CX extract was obtained from the culture medium of Escherichia coli/pAC-CANTHipi. The extracts were classified into five concentrations (CX1, CX3, CX5, CX7, and CX10) based on different volume ratios between culture broth and extraction solvent (1:1, 3:1, 5:1, 7:1, 10:1), and then used for ex situ modification of FBC to obtain FBC/CX composite films. The results demonstrated the successful preparation of FBC and its subsequent modification with CX. The modified FBC/CX films exhibited a concentration-dependent orange-red appearance. Antioxidant analysis confirmed that CX extracts from CX1 to CX7 displayed a good linear increase in antioxidant activity. Specifically, the FBC/CX10 film exhibited a Trolox equivalent of 4.86 ± 0.73 mg/g and a free radical scavenging rate of 21.30 ± 1.43 %, significantly higher than the unmodified FBC (1.10 ± 0.202 mg/g and 3.30 ± 0.74 %). Furthermore, cytocompatibility assays confirmed that all films exhibited good biocompatibility with NIH-3T3 cells. In the refrigerated packaging test using fish fillets, the FBC/CX10 film showed excellent antimicrobial and antioxidant effects. Total viable count analysis revealed that FBC/CX10 reduced microbial growth by 73.95 % on day 3 compared to the blank group, and by 76.52 % and 77.62 % on days 9 and 12, respectively. Regarding lipid oxidation, the malondialdehyde (MDA) content in FBC/CX10 on day 3 was 0.68 ± 0.05 mg/g, significantly lower than that of the blank (1.25 ± 0.49 mg/g) and FBC (1.52 ± 0.16 mg/g) groups, indicating that FBC/CX10 films effectively suppressed lipid oxidation in the early stages of cold storage. These findings confirm that FBC/CX10 films have high potential as active materials for food packaging applications, capable of delaying microbial spoilage and oxidative degradation. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2026-08-24T16:05:46Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2026-08-24T16:05:46Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 謝誌 i
摘要 ii Abstract iii 目次 v 圖次 x 表次 xi List of Figures xii List of Tables xiv 壹、前言 1 貳、文獻回顧 2 2.1 食品包裝 2 2.1.1 食品包裝技術 4 2.1.2食品包裝現況與挑戰 4 2.1.2.1 食品浪費與食安問題現況與因應 4 2.1.2.2 生物性危害 5 2.1.1.3 化學性危害 5 2.1.2.4 物理性危害 6 2.1.2.5 相關法規 7 2.1.3 傳統包裝材料的限制與環境衝擊 9 2.1.4 食品保存需求與功能性包裝興起 11 2.1.5 活性與智能包裝的機制與應用 16 2.1.5.1活性包裝的原理與應用 16 2.1.5.2 智能包裝的系統與應用 18 2.2 生物塑膠 20 2.2.1 生物基塑膠 21 2.2.2 可生物降解材料於食品包裝之應用 22 2.3 細菌性纖維簡介 25 2.3.1 細菌性纖維素的合成原理與生產菌株 25 2.3.2 細菌性纖維素材料特性與應用潛力 26 2.3.3 細菌性纖維素之應用 28 2.3.3.1 生物醫學材料應用 28 2.3.3.2 環境應用 29 2.3.3.3 食品包裝應用 29 2.4 細菌性纖維修飾 32 2.4.1 原位修飾 32 2.4.2 異位修飾 33 2.4.3 泡沫狀細菌性纖維素的製備與特性 36 2.4.4 泡沫狀細菌性纖維之修飾應用 39 2.5 角黃素之簡介 40 2.5.1 類胡蘿蔔素之介紹 41 2.5.2 角黃素之生合成路徑與穩定性 43 2.5.3 角黃素之抗氧化機制與比較分析 44 2.5.4 角黃素於食品、醫療與健康之應用 48 參、研究目的與架構 50 3.1 研究目的 50 肆、材料與方法 52 4.1 實驗材料 52 4.1.1 實驗菌種 52 4.1.2 實驗藥品 52 4.2 儀器設備 53 4.3 實驗方法 54 4.3.1 培養基配製 54 4.3.1.1 大腸桿菌 LB 培養基配製 54 4.3.1.2 細菌纖維素 CSL-fructose 培養基配製 54 4.3.1.3 泡沫狀細菌性纖維素 modified CSL-fructose 培養基配製 54 4.3.2 細菌性纖維素製備 55 4.3.2.1凍管製作 55 4.3.2.2 木質醋酸菌菌株活化 55 4.3.2.3 泡沫狀細菌性纖維素薄膜製備 56 4.3.2.4 泡沫狀細菌性纖維素薄膜純化與冷凍乾燥 56 4.3.3 角黃素製備 56 4.3.3.1 重組菌株凍管製備與保存 56 4.3.3.2基因工程轉殖與質體確認 57 4.3.3.3重組菌株種菌培養 57 4.3.3.4 重組菌株之色素生產與角黃素萃取 57 4.3.4 角黃素異位修飾之泡沫狀細菌性纖維素複合膜製備 58 4.3.5 材料特性分析 58 4.3.5.1 吸附能力分析 58 4.3.5.2 分光色差儀色度分析 59 4.3.5.3 掃描式電子顯微鏡分析 59 4.3.5.4 傅立葉紅外線光譜分析 60 4.3.5.5 X 光繞射分析 60 4.3.5.6 熱重分析 60 4.3.6 抗氧化實驗 61 4.3.7 食品包裝實驗 62 4.3.7.1 細胞毒性分析 62 4.3.7.2金目鱸魚儲藏性實驗 62 4.3.7.2.1 總生菌數 63 4.3.7.2.2 總揮發性鹼性氮 63 4.3.7.2.3 硫代巴比妥酸反應物質 64 4.3.8 統計分析 65 伍、結果與討論 66 5.1 泡沫狀細菌性纖維素製備與純化 66 5.2. 重組菌株之色素生產與角黃素萃取 68 5.3 角黃素異位修飾之泡沫狀細菌性纖維素複合膜製備 70 5.4 吸附能力分析 72 5.5分光色差儀色度分析 74 5.6 掃描式電子顯微鏡分析 76 5.7 傅立葉紅外線光譜分析 78 5.8 X 光繞射分析 80 5.9 熱重分析 82 5.10 抗氧化能力分析 84 5.10.1 角黃素萃取液抗氧化能力分析 84 5.10.2 角黃素異位修飾泡沫狀細菌性纖維素複合膜抗氧化能力分析 87 5.11 細胞實驗 90 5.12 金目鱸魚儲藏性實驗 92 5.12.1 總生菌數 94 5.12.2 總揮發性鹼性氮 97 5.12.3 硫代巴比妥酸反應物質 99 陸、結論 101 柒、未來展望 103 捌、參考文獻 104 玖、附錄 124 9.1 培養基成分表 124 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 細菌性纖維素 | - |
| dc.subject | 角黃素 | - |
| dc.subject | 活性包裝 | - |
| dc.subject | 抗氧化 | - |
| dc.subject | 異位修飾 | - |
| dc.subject | Foaming bacterial cellulose | - |
| dc.subject | Canthaxanthin | - |
| dc.subject | Active food packaging | - |
| dc.subject | Antioxidant | - |
| dc.subject | Ex situ modification | - |
| dc.title | 角黃素異位修飾泡沫狀細菌性纖維素用於食品活性包裝之開發 | zh_TW |
| dc.title | Foaming bacterial cellulose incorporated with canthaxanthin via ex situ modification for active food packaging applications | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 114-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 林欣平;張文昌;王鐘毅;鄭孟純 | zh_TW |
| dc.contributor.oralexamcommittee | SHIN-PING LIN;WEN-CHANG CHANG;CHUNG-YI WANG;MENG-CHUN CHENG | en |
| dc.subject.keyword | 細菌性纖維素; 角黃素; 活性包裝; 抗氧化; 異位修飾 | zh_TW |
| dc.subject.keyword | Foaming bacterial cellulose; Canthaxanthin; Active food packaging; Antioxidant; Ex situ modification | en |
| dc.relation.page | 124 | - |
| dc.identifier.doi | 10.6342/NTU202603324 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2026-08-19 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 生物科技研究所 | - |
| dc.date.embargo-lift | 2026-08-25 | - |
| 顯示於系所單位： | 生物科技研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-114-2.pdf | 3.05 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。