請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/101733完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 王永松 | zh_TW |
| dc.contributor.advisor | Yung-Song Wang | en |
| dc.contributor.author | 江潔欣 | zh_TW |
| dc.contributor.author | Chieh-Hsin Chiang | en |
| dc.date.accessioned | 2026-03-04T16:08:25Z | - |
| dc.date.available | 2026-03-05 | - |
| dc.date.copyright | 2026-03-04 | - |
| dc.date.issued | 2026 | - |
| dc.date.submitted | 2026-02-25 | - |
| dc.identifier.citation | Arndt A, C Marquez, P Lambert, MJ Smith. (1996). Molecular phylogeny of eastern Pacific sea cucumbers (Echinodermata: Holothuroidea) based on mitochondrial DNA sequence. Molecular Phylogenetics and Evolution 6: 425-437.
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Les Holothuries Aspidochirotes du lagon de Nouvelle-Calédonie: biologie, écologie et exploitation (Vol. 42). Ed. de l'ORSTOM. Cutress BM. (1996). Changes in dermal ossicles during somatic growth in Caribbean littoral sea cucumbers (Echinoidea: Holothuroidea: Aspidochirotida). Bulletin of Marine Science, 58(1), 44-116. Froese R. (2006). Cube law, condition factor and weight-length relationships: History, meta-analysis and recommendations. Journal of Applied Ichthyology 22: 241-253. Hajibabaei M, GA Singer, PDN Hebert, DA Hickey. (2007). DNA barcoding: how it complements taxonomy, molecular phylogenetics, and population genetics. Trends in Genetics, 23(4), 167–172. Havird JC, SR Santos. (2014). Performance of single and concatenated sets of mitochondrial genes at inferring metazoan relationships relative to full mitogenome data. PLOS ONE, 9(1), e84080. Hebert PDN, A Cywinska, SL Ball, JR deWaard. (2003). Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270(1512), 313–321. Honey-Escandón M, FA Solís-Marín, A Laguarda-Figueras. (2011). Holothuria (Selenkothuria) carere, a new species of sea cucumber (Echinodermata: Holothuroidea) from the Mexican Pacific. Zootaxa 2922: 27-33. Jia C, Z Xu, F Li, L Chen, A Wang, F Gao. (2025). Taxonomic status and phylogenetic analyses based on complete mitochondrial genome and microscopic ossicles: Redescription of a controversial tropical sea cucumber species (Holothuroidea, Holothuria Linnaeus, 1767). Zoosystematics and Evolution, 100(1), 137–152. Khatulistiani TS, AS Dewi, Yasman. (2022). Detailed description of scanning electron microscope (SEM) of the Holothuria scabra’s ossicles (Holothuroidea: Echinodermata) collected from Pesawaran Waters, Lampung, Indonesia. Biodiversitas, 23(7), 3697–3704. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. Kuo CY, S Keshavmurthy, YY , MJ Ho, HJ Hsieh, AT Hsiao, WC Lo, YC Hsin, CA Chen. (2023). Transitional coral ecosystem of Taiwan in the era of changing climate in Coral Reefs of Eastern Asia under Anthropogenic Impacts pp. 7-35. Lee, S., Ferse, S. C., Ford, A., Wild, C., & Mangubhai, S. (2017). Effect of sea cucumber density on the health of reef-flat sediments. Wildlife Conservation Society. Madruga AS, PM Felix, J Sousa, FAE Silva, AC Brito, S Mendes, Pombo. (2023). Effect of rearing temperature in the growth of hatchery reared juveniles of the sea cucumber (Koehler & Vaney, 1906). Aquaculture 562. Massin C. 1996. Results of the rumphius biohistorical expedition to ambon (1990). Part. 4. The Holothurioidea (Echinodermata) collected at ambon during the rumphius biohistorical expedition. Zoologische Verhandelingen 307: 53 pp. Massin C. 2013. Holothuria (selenkothuria) parvispinea sp. nov.. (Echinodermata, Hololthuroidea, Holothuriidae) with key to the sub-genus selenkothuria. Zootaxa 3609: 343-348. Miller, A. K., Kerr, A. M., Paulay, G., Reich, M., Wilson, N. G., Carvajal, J. I., & Rouse, G. W. (2017). Molecular phylogeny of extant Holothuroidea (Echinodermata). Molecular Phylogenetics and Evolution, 111, 110-131. Morey, G., Moranta, J., Massutı, E., Grau, A., Linde, M., Riera, F., & Morales-Nin, B. (2003). Weight–length relationships of littoral to lower slope fishes from the western Mediterranean. Fisheries Research, 62(1), 89-96. Morgan B, TY Wang, YZ Chen, V Moctezuma, O Burgos, MH Le, JP Huang. (2022). Long-read sequencing data reveals dynamic evolution of mitochondrial genome size and the phylogenetic utility of mitochondrial DNA in Hercules beetles (Dynastes; Scarabaeidae). Genome Biology and Evolution, 14(10), evac147. Pan, W., Wang, X., Ren, C., Jiang, X., Gong, S., Xie, Z., ... & Chen, T. (2024). Sea cucumbers and their symbiotic microbiome have evolved to feed on seabed sediments. Nature Communications, 15(1), 1-14. Pasquini V, P Addis, AA Giglioli, D Moccia, A Pusceddu. (2023). Outcomes of feeding activity of the sea cucumber Holothuria tubulosa on quantity, biochemical composition, and nutritional quality of sedimentary organic matter. Frontiers in Marine Science, 9:1010014. Perna, N. T., & Kocher, T. D. (1995). Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes. Journal of molecular evolution, 41(3), 353-358. Perseke M, D Bernhard, G Fritzsch, F Brummer, PF Stadler, M Schlegel. (2010). Mitochondrial genome evolution in Ophiuroidea, Echinoidea, and Holothuroidea: Insights in phylogenetic relationships of Echinodermata. Molecular Phylogenetics and Evolution 56: 201-211. Purcell SW, H Gossuin, NS Agudo. (2009). Status and Management of the Sea Cucumber Fishery of La Grande Terre, New Caledonia. Programme ZoN´eCo, WorldFish Center Studies and Reviews 1901. The WorldFish Center, Malaysia, p. 136. Rowe FWE. (1969). A review of the family Holothuriidae (Holothuroidea: Aspidochirotida). Bulletin of the British Museum (Natural History) Zoology 18: 119–170. Samyn Y, D Vandenspiegel, C Massin. (2006). Taxonomie des holothuries des. Comores. Abc Taxa 1. Samyn Y, D Vandenspiegel. (2004). An introduction to the taxonomy of Holothuroidea. Royal Belgian Institute of Natural Sciences. Smirnov, AV. 2014. Sea cucumbers symmetry (Echinodermata: Holothuroidea). Paleontological Journal, 48(12), 1523–1534. Tamura K, G Stecher, S Kumar. (2021). Mega11 molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38: 3022-3027. Tersol JN, MV Gabuyo, LMM Palmones, FD Manipon, JRD Torres. (2023). Ossicle morphology of Sandfish (Holothuria scabra Jaeger) at different stages of maturity. International Journal of Biosciences, 23(2), 240–254. Thandar AS. (1988). A new subgenus of Holothuria with a description of a new species from the Southeast Atlantic Ocean. Journal of Zoology 215: 47-54. Uthicke S, M Byrne, C Conand. (2010). Genetic barcoding of commercial Beche-de-mer species (Echinodermata: Holothuroidea). Molecular Ecology Resources 10(4), 634-646. Wang, Z., Wang, Z., Shi, X., Wu, Q., Tao, Y., Guo, H., ... & Bai, Y. (2018). Complete mitochondrial genome of Parasesarma affine (Brachyura: Sesarmidae): Gene rearrangements in Sesarmidae and phylogenetic analysis of the Brachyura. International journal of biological macromolecules, 118, 31-40. 趙世民. 1998. 臺灣礁岩海岸的海參. 臺中市:國立自然科學博物館. 廖玉麟. 1997. 中國動物誌 棘皮動物門 海參綱. 北京:科學出版社. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/101733 | - |
| dc.description.abstract | 本研究闡述一種在臺東海岸發現的新物種海參，其外觀特徵為黑色的圓柱形身體、柔軟的表皮，腹側具有呈三縱列排列的管足，背側有許多均勻分佈的疣足，口部具約20個呈樹枝狀的攝食觸手，收縮時呈楯狀；利用掃描式電子顯微鏡觀察，在管足、觸手、縱肌以及體背側可發現棒狀和末端分支的棒狀骨針；解剖構造顯示其缺乏居維葉氏管，上述特徵表明此海參應為尚未發現的物種。此新種海參的粒線體基因體共有15,720個鹼基對，由37個基因構成，包括13個蛋白質編碼基因、2個核糖體RNA基因、22個轉運RNA基因，以及一個非編碼控制區；粒線體基因體的AT-skew (0.04) 和GC-skew (−0.17) 分別為正值和負值，與大多數已定序的棘皮動物相同。親緣關係分析顯示此新種海參位於海參科物種構成的單一支序群，與同屬海參目的刺參科分為不同支系。本研究結合形態和分子證據，將該物種分類於海參目海參科，並透過其獨特的骨針組成，將其歸類至海參屬下的賽倫參亞屬。本研究亦分析此海參的體長與體重關係，顯示此海參為負異速生長，與在其他海參中觀察到的生長模式相似。本研究首次發現並闡述一未被記錄的海參物種，提出新的學名，且為臺灣定序海參完整粒線體基因體之先驅，並進行野外族群調查，提供關於此新物種的生態棲位和演化關係的見解。 | zh_TW |
| dc.description.abstract | This study describes a newly discovered sea cucumber species from the eastern coast of Taiwan and focuses on its morphological characteristics, growth patterns, and complete mitochondrial genome. The species is characterized by a black, cylindrical body and soft skin. Tube feet are arranged in three adjacent rows, and the dorsal side is covered with multiple evenly distributed papillae. The species has approximately 20 dendritic tentacles that appear peltate-like when contracted. Scanning electron microscopy showed that only rod-shaped or terminally branched rod-shaped ossicles are present in the tube feet, tentacles, longitudinal muscles, and dorsal body. Internal anatomy shows the absence of Cuvierian organs. The complete mitogenome comprises 15,720 base pairs and contains 37 genes, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a single control region. The AT-skew (0.04) and GC-skew (−0.17) in the mitogenome are positive and negative, respectively, consistent with most sequenced echinoderms. Phylogenetic analysis based on mitochondrial amino acid sequences places this species within the Holothuriidae family. Based on both morphological and molecular data, the new species is classified as Holothuriida: Holothuriidae. The dermal ossicle compositions support its classification as subgenus Selenkothuria of Holothuria. The length-weight relationship index and growth coefficient indicate negative allometric growth, similar to patterns observed in other holothurians. Our research offers insights into the ecology and evolution of a previously unrecognized sea cucumber species. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2026-03-04T16:08:25Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2026-03-04T16:08:25Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 i
中文摘要 ii Abstract iii 目次 iv 表次 vi 圖次 vii 第一章 前言 1 1.1海參的生態棲位與產業概況 1 1.2 海參的構造與觀察 2 1.3 海參的命名與分類 4 1.4物種鑑定方法與親緣關係分析 6 1.5新物種的鑑定與發表 8 1.6 研究目的 9 第二章 材料與方法 11 2.1樣本來源與保存 11 2.2形態學研究 11 2.3 DNA萃取與粒線體全基因體定序 12 2.4 序列分析與基因註解 (Gene Annotation) 12 2.5 親緣演化分析 13 2.6 命名學處理與資料庫註冊 15 2.7 體長與體重數據分析 15 笫三章 實驗結果 17 3.1 物種描述 17 3.2生物分類 (Taxonomy) 18 3.2.1 分類歸屬 18 3.2.2 物種界定 (Species Delimitation) 19 3.3 粒線體基因體註解 21 3.4 系統發育分析 (Phylogenetic Analysis) 22 3.5 命名與註冊 24 3.6 體長、體重之測量與族群生長模式 25 第四章 討論 27 4.1 亞屬內物種比較與趨同演化 27 4.2 分子親緣關係與特徵演化 28 4.3 族群生長模式與生態適應 30 第五章 結論 32 參考文獻 33 表次 表一.臺灣記錄的海參種類 37 表二.新種海參之完整粒線體基因體註解 39 表三. 14種海參的完整線粒體DNA的鹼基組成列表 41 表四. 2024-2025年臺灣東海岸海參的生長性能及參數 42 表五.亞屬內海參物種特徵比較 43 圖次 圖一. 新種海參 (Holothuria ciliksiz sp. nov.) 採集座標及生態棲地 44 圖二. 新種海參 (Holothuria ciliksiz sp. nov.) 活體形態與解剖構造觀察 45 圖三. 新種海參 (Holothuria ciliksiz sp. nov.) 各部位的桿狀骨針 46 圖四、新種海參於野外觀察到的斷裂生殖 ...47 圖五、新種海參 (Holothuria ciliksiz sp. nov.) 與黑赤星海參 48 圖六、與新種海參與外觀相似的海參屬物種之骨針示意圖 49 圖七. 新種海參 (Holothuria ciliksiz sp. nov.) 粒線體基因體之基因圖譜 50 圖八. 新種海參 (Holothuria ciliksiz sp. nov.) 粒線體蛋白質編碼基因註解 51 圖九. 海參粒線體基因體序列之分子親緣關係樹 52 圖十. 臺灣臺東沿海海參周年調查之每月體長及體重分布與常態性 53 圖十一. 臺灣臺東沿海海參體長體重周年調查與生長模式分析 55 圖十二. 臺灣臺東沿海月度海表溫度與海參生長係數關係圖 56 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 棘皮動物門 | - |
| dc.subject | 海參科 | - |
| dc.subject | 海參屬 | - |
| dc.subject | 新種 | - |
| dc.subject | 骨針 | - |
| dc.subject | 粒線體基因體 | - |
| dc.subject | 體重與體長關係 | - |
| dc.subject | Echinodermata | - |
| dc.subject | Holothuroidae | - |
| dc.subject | Holothuria (Selenkothuria) | - |
| dc.subject | new species | - |
| dc.subject | ossicle | - |
| dc.subject | mitogenome | - |
| dc.subject | length-weight relationship | - |
| dc.title | 臺灣東部海岸之海參新種：形態特徵、粒線體基因體與生長模式分析 | zh_TW |
| dc.title | A New Species of Holothuria (Echinodermata: Holothuriidae) from Taiwan’s Eastern Coast: Morphology, Mitochondrial Genome and Growth Pattern | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 114-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 曾登裕;何瓊紋 | zh_TW |
| dc.contributor.oralexamcommittee | Deng-Yu Tseng;Chuan-Wen Ho | en |
| dc.subject.keyword | 棘皮動物門,海參科海參屬新種骨針粒線體基因體體重與體長關係 | zh_TW |
| dc.subject.keyword | Echinodermata,HolothuroidaeHolothuria (Selenkothuria)new speciesossiclemitogenomelength-weight relationship | en |
| dc.relation.page | 56 | - |
| dc.identifier.doi | 10.6342/NTU202600500 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2026-02-25 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 漁業科學研究所 | - |
| dc.date.embargo-lift | 2026-03-05 | - |
| 顯示於系所單位： | 漁業科學研究所 | |
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