請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10103
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 邱繼輝(Kay-Hooi Khoo) | |
dc.contributor.author | Shui-Hua Wang | en |
dc.contributor.author | 王穗華 | zh_TW |
dc.date.accessioned | 2021-05-20T21:02:09Z | - |
dc.date.available | 2014-07-27 | |
dc.date.available | 2021-05-20T21:02:09Z | - |
dc.date.copyright | 2011-07-27 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-07-19 | |
dc.identifier.citation | Abbott, K.L., Aoki, K., Lim, J.M., Porterfield, M., Johnson, R., O'Regan, R.M., Wells, L., Tiemeyer, M., and Pierce, M. (2008a). Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J Proteome Res 7, 1470-1480.
Abbott, K.L., Nairn, A.V., Hall, E.M., Horton, M.B., McDonald, J.F., Moremen, K.W., Dinulescu, D.M., and Pierce, M. (2008b). Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics 8, 3210-3220. Acosta-Rodriguez, E.V., Montes, C.L., Motran, C.C., Zuniga, E.I., Liu, F.T., Rabinovich, G.A., and Gruppi, A. (2004). Galectin-3 mediates IL-4-induced survival and differentiation of B cells: functional cross-talk and implications during Trypanosoma cruzi infection. J Immunol 172, 493-502. Agrawal, B.B., and Goldstein, I.J. (1965). Specific binding of concanavalin A to cross-linked dextran gels. Biochem J 96, 23contd-25c. Amano, M., Galvan, M., He, J., and Baum, L.G. (2003). The ST6Gal I sialyltransferase selectively modifies N-glycans on CD45 to negatively regulate galectin-1-induced CD45 clustering, phosphatase modulation, and T cell death. J Biol Chem 278, 7469-7475. Angata, K., and Fukuda, M. (2003). Polysialyltransferases: major players in polysialic acid synthesis on the neural cell adhesion molecule. Biochimie 85, 195-206. Angata, K., Suzuki, M., and Fukuda, M. (1998). Differential and cooperative polysialylation of the neural cell adhesion molecule by two polysialyltransferases, PST and STX. J Biol Chem 273, 28524-28532. Angata, T., and Varki, A. (2002). Chemical diversity in the sialic acids and related alpha-keto acids: an evolutionary perspective. Chem Rev 102, 439-469. Aoki, K., Perlman, M., Lim, J.M., Cantu, R., Wells, L., and Tiemeyer, M. (2007). Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo. J Biol Chem 282, 9127-9142. Atrih, A., Richardson, J.M., Prescott, A.R., and Ferguson, M.A. (2005). Trypanosoma brucei glycoproteins contain novel giant poly-N-acetyllactosamine carbohydrate chains. J Biol Chem 280, 865-871. Avril, T., North, S.J., Haslam, S.M., Willison, H.J., and Crocker, P.R. (2006). Probing the cis interactions of the inhibitory receptor Siglec-7 with alpha2,8-disialylated ligands on natural killer cells and other leukocytes using glycan-specific antibodies and by analysis of alpha2,8-sialyltransferase gene expression. J Leukoc Biol 80, 787-796. Babu, P., North, S.J., Jang-Lee, J., Chalabi, S., Mackerness, K., Stowell, S.R., Cummings, R.D., Rankin, S., Dell, A., and Haslam, S.M. (2009). Structural characterisation of neutrophil glycans by ultra sensitive mass spectrometric glycomics methodology. Glycoconj J 26, 975-986. Bateman, A.C., Karamanska, R., Busch, M.G., Dell, A., Olsen, C.W., and Haslam, S.M. (2010). Glycan analysis and influenza A virus infection of primary swine respiratory epithelial cells: the importance of NeuAc{alpha}2-6 glycans. J Biol Chem 285, 34016-34026. Baum, L.G., Derbin, K., Perillo, N.L., Wu, T., Pang, M., and Uittenbogaart, C. (1996). Characterization of terminal sialic acid linkages on human thymocytes. Correlation between lectin-binding phenotype and sialyltransferase expression. J Biol Chem 271, 10793-10799. Bierhuizen, M.F., Mattei, M.G., and Fukuda, M. (1993). Expression of the developmental I antigen by a cloned human cDNA encoding a member of a beta-1,6-N-acetylglucosaminyltransferase gene family. Genes Dev 7, 468-478. Brewer, C.F., Miceli, M.C., and Baum, L.G. (2002). Clusters, bundles, arrays and lattices: novel mechanisms for lectin-saccharide-mediated cellular interactions. Curr Opin Struct Biol 12, 616-623. Callaghan, J.M., Toh, B.H., Pettitt, J.M., Humphris, D.C., and Gleeson, P.A. (1990). Poly-N-acetyllactosamine-specific tomato lectin interacts with gastric parietal cells. Identification of a tomato-lectin binding 60-90 X 10(3) Mr membrane glycoprotein of tubulovesicles. J Cell Sci 95 ( Pt 4), 563-576. Callaghan, J.M., Toh, B.H., Simpson, R.J., Baldwin, G.S., and Gleeson, P.A. (1992). Rapid purification of the gastric H+/K(+)-ATPase complex by tomato-lectin affinity chromatography. Biochem J 283 ( Pt 1), 63-68. Canis, K., McKinnon, T.A., Nowak, A., Panico, M., Morris, H.R., Laffan, M., and Dell, A. (2010). The plasma von Willebrand factor O-glycome comprises a surprising variety of structures including ABH antigens and disialosyl motifs. J Thromb Haemost 8, 137-145. Carlsson, S.R., and Fukuda, M. (1990). The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2. Localization on the peptide backbones. J Biol Chem 265, 20488-20495. Chakraborty, A.K., and Pawelek, J.M. (2003). GnT-V, macrophage and cancer metastasis: a common link. Clin Exp Metastasis 20, 365-373. Chen, H., and He, M. (2005). Quantitation of synthetic polymers using an internal standard by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Am Soc Mass Spectrom 16, 100-106. Chen, S.C., Huang, B., Liu, Y.C., Shyu, K.G., Lin, P.Y., and Wang, D.L. (2008). Acute hypoxia enhances proteins' S-nitrosylation in endothelial cells. Biochem Biophys Res Commun 377, 1274-1278. Cheresh, D.A., Pierschbacher, M.D., Herzig, M.A., and Mujoo, K. (1986). Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins. J Cell Biol 102, 688-696. Chokhawala, H.A., Yu, H., and Chen, X. (2007). High-throughput substrate specificity studies of sialidases by using chemoenzymatically synthesized sialoside libraries. Chembiochem 8, 194-201. Ciucanu, I., and Kerek, F. (1984). A simple and rapid method for the permethylation of carbohydrates. Carbohydrate Research 131, 209-217. Collins, B.E., Blixt, O., DeSieno, A.R., Bovin, N., Marth, J.D., and Paulson, J.C. (2004). Masking of CD22 by cis ligands does not prevent redistribution of CD22 to sites of cell contact. Proc Natl Acad Sci U S A 101, 6104-6109. Costello, B.D.a.C.E. (1988). A systematic nomenclature for carbohydrate fragmentations in FAB-MS/MS spectra of glycoconjugates Glycoconjugate Journal 5, 397-409. Cummings, R.D., and Kornfeld, S. (1982). Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins. J Biol Chem 257, 11230-11234. Cummings, R.D., and Kornfeld, S. (1984). The distribution of repeating [Gal beta 1,4GlcNAc beta 1,3] sequences in asparagine-linked oligosaccharides of the mouse lymphoma cell lines BW5147 and PHAR 2.1. J Biol Chem 259, 6253-6260. Cummings, R.D., Trowbridge, I.S., and Kornfeld, S. (1982). A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase. J Biol Chem 257, 13421-13427. Dang, A.M., Phillips, J.A., Lin, T., and Raveche, E.S. (1996). Altered CD45 expression in malignant B-1 cells. Cell Immunol 169, 196-207. Dell, A. (1987). F.A.B.-mass spectrometry of carbohydrates. Adv Carbohydr Chem Biochem 45, 19-72. Dell, A., Reason, A.J., Khoo, K.H., Panico, M., McDowell, R.A., and Morris, H.R. (1994). Mass spectrometry of carbohydrate-containing biopolymers. Methods Enzymol 230, 108-132. Demetriou, M., Granovsky, M., Quaggin, S., and Dennis, J.W. (2001). Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation. Nature 409, 733-739. Denecke, J., Kranz, C., Nimtz, M., Conradt, H.S., Brune, T., Heimpel, H., and Marquardt, T. (2008). Characterization of the N-glycosylation phenotype of erythrocyte membrane proteins in congenital dyserythropoietic anemia type II (CDA II/HEMPAS). Glycoconj J 25, 375-382. Dennis, J.W., Granovsky, M., and Warren, C.E. (1999). Glycoprotein glycosylation and cancer progression. Biochim Biophys Acta 1473, 21-34. Dennis, J.W., Laferte, S., Waghorne, C., Breitman, M.L., and Kerbel, R.S. (1987). Beta 1-6 branching of Asn-linked oligosaccharides is directly associated with metastasis. Science 236, 582-585. Dennis, J.W., Nabi, I.R., and Demetriou, M. (2009). Metabolism, cell surface organization, and disease. Cell 139, 1229-1241. Diamandis, E.P. (2004). Mass spectrometry as a diagnostic and a cancer biomarker discovery tool: opportunities and potential limitations. Mol Cell Proteomics 3, 367-378. Donnelly, E.H., and Goldstein, I.J. (1970). Glutaraldehyde-insolubilized concanavalin A: an adsorbent for the specific isolation of polysaccharides and glycoproteins. Biochem J 118, 679-680. Earl, L.A., Bi, S., and Baum, L.G. (2010). N- and O-glycans modulate galectin-1 binding, CD45 signaling, and T cell death. J Biol Chem 285, 2232-2244. Edgell, C.J., McDonald, C.C., and Graham, J.B. (1983). Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci U S A 80, 3734-3737. Emeis, J.J., and Edgell, C.J. (1988). Fibrinolytic properties of a human endothelial hybrid cell line (Ea.hy 926). Blood 71, 1669-1675. Espeli, M., Mancini, S.J., Breton, C., Poirier, F., and Schiff, C. (2009). Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions. Blood 113, 5878-5886. Fan, Y.Y., Yu, S.Y., Ito, H., Kameyama, A., Sato, T., Lin, C.H., Yu, L.C., Narimatsu, H., and Khoo, K.H. (2008). Identification of further elongation and branching of dimeric type 1 chain on lactosylceramides from colonic adenocarcinoma by tandem mass spectrometry sequencing analyses. J Biol Chem 283, 16455-16468. Finne, J., Krusius, T., and Rauvala, H. (1977a). Occurrence of disialosyl groups in glycoproteins. Biochem Biophys Res Commun 74, 405-410. Finne, J., Krusius, T., Rauvala, H., and Hemminki, K. (1977b). The disialosyl group of glycoproteins. Occurrence in different tissues and cellular membranes. Eur J Biochem 77, 319-323. Freeze, H.H., and Aebi, M. (2005). Altered glycan structures: the molecular basis of congenital disorders of glycosylation. Curr Opin Struct Biol 15, 490-498. Fukuda, M., Bothner, B., Ramsamooj, P., Dell, A., Tiller, P.R., Varki, A., and Klock, J.C. (1985). Structures of sialylated fucosyl polylactosaminoglycans isolated from chronic myelogenous leukemia cells. J Biol Chem 260, 12957-12967. Fukuda, M., Dell, A., and Fukuda, M.N. (1984a). Structure of fetal lactosaminoglycan. The carbohydrate moiety of Band 3 isolated from human umbilical cord erythrocytes. J Biol Chem 259, 4782-4791. Fukuda, M., Dell, A., Oates, J.E., and Fukuda, M.N. (1984b). Structure of branched lactosaminoglycan, the carbohydrate moiety of band 3 isolated from adult human erythrocytes. J Biol Chem 259, 8260-8273. Fukuda, M., Fukuda, M.N., and Hakomori, S. (1979). Developmental change and genetic defect in the carbohydrate structure of band 3 glycoprotein of human erythrocyte membrane. J Biol Chem 254, 3700-3703. Fukuda, M., Guan, J.L., and Rose, J.K. (1988). A membrane-anchored form but not the secretory form of human chorionic gonadotropin-alpha chain acquires polylactosaminoglycan. J Biol Chem 263, 5314-5318. Fukuda, M., Lauffenburger, M., Sasaki, H., Rogers, M.E., and Dell, A. (1987). Structures of novel sialylated O-linked oligosaccharides isolated from human erythrocyte glycophorins. J Biol Chem 262, 11952-11957. Fukuda, M., Spooncer, E., Oates, J.E., Dell, A., and Klock, J.C. (1984c). Structure of sialylated fucosyl lactosaminoglycan isolated from human granulocytes. J Biol Chem 259, 10925-10935. Fukuda, M.N., and Matsumura, G. (1976). Endo-beta-galactosidase of Escherichia freundii. Purification and endoglycosidic action on keratan sulfates, oligosaccharides, and blood group active glycoprotein. J Biol Chem 251, 6218-6225. Fukuda, M.N., Sasaki, H., Lopez, L., and Fukuda, M. (1989). Survival of recombinant erythropoietin in the circulation: the role of carbohydrates. Blood 73, 84-89. Garcia-Vallejo, J.J., Van Dijk, W., Van Het Hof, B., Van Die, I., Engelse, M.A., Van Hinsbergh, V.W., and Gringhuis, S.I. (2006). Activation of human endothelial cells by tumor necrosis factor-alpha results in profound changes in the expression of glycosylation-related genes. J Cell Physiol 206, 203-210. Garcia, G.G., Berger, S.B., Sadighi Akha, A.A., and Miller, R.A. (2005). Age-associated changes in glycosylation of CD43 and CD45 on mouse CD4 T cells. Eur J Immunol 35, 622-631. Garner, O.B., and Baum, L.G. (2008). Galectin-glycan lattices regulate cell-surface glycoprotein organization and signalling. Biochem Soc Trans 36, 1472-1477. Gauthier, L., Rossi, B., Roux, F., Termine, E., and Schiff, C. (2002). Galectin-1 is a stromal cell ligand of the pre-B cell receptor (BCR) implicated in synapse formation between pre-B and stromal cells and in pre-BCR triggering. Proc Natl Acad Sci U S A 99, 13014-13019. Gillespie, W., Kelm, S., and Paulson, J.C. (1992). Cloning and expression of the Gal beta 1, 3GalNAc alpha 2,3-sialyltransferase. J Biol Chem 267, 21004-21010. Gu, J., Nishikawa, A., Fujii, S., Gasa, S., and Taniguchi, N. (1992). Biosynthesis of blood group I and i antigens in rat tissues. Identification of a novel beta 1-6-N-acetylglucosaminyltransferase. J Biol Chem 267, 2994-2999. Guo, H.B., Randolph, M., and Pierce, M. (2007). Inhibition of a specific N-glycosylation activity results in attenuation of breast carcinoma cell invasiveness-related phenotypes: inhibition of epidermal growth factor-induced dephosphorylation of focal adhesion kinase. J Biol Chem 282, 22150-22162. Hakomori, S. (1964). A Rapid Permethylation of Glycolipid, and Polysaccharide Catalyzed by Methylsulfinyl Carbanion in Dimethyl Sulfoxide. J Biochem 55, 205-208. Hara, S., Takemori, Y., Yamaguchi, M., Nakamura, M., and Ohkura, Y. (1987). Fluorometric high-performance liquid chromatography of N-acetyl- and N-glycolylneuraminic acids and its application to their microdetermination in human and animal sera, glycoproteins, and glycolipids. Anal Biochem 164, 138-145. Harvey, D.J. (2000). Postsource decay fragmentation of N-linked carbohydrates from ovalbumin and related glycoproteins. J Am Soc Mass Spectrom 11, 572-577. Harvey, D.J., Bateman, R.H., Bordoli, R.S., and Tyldesley, R. (2000). Ionisation and fragmentation of complex glycans with a quadrupole time-of-flight mass spectrometer fitted with a matrix-assisted laser desorption/ionisation ion source. Rapid Commun Mass Spectrom 14, 2135-2142. Heinz Egge, J.P.-K. (1987). Fast atom bombardment mass spectrometry for structural elucidation of glycoconjugates. 6, 331-393. Hirabayashi, J., Hashidate, T., Arata, Y., Nishi, N., Nakamura, T., Hirashima, M., Urashima, T., Oka, T., Futai, M., Muller, W.E., et al. (2002). Oligosaccharide specificity of galectins: a search by frontal affinity chromatography. Biochim Biophys Acta 1572, 232-254. Hirabayashi, J., Satoh, M., and Kasai, K. (1992). Evidence that Caenorhabditis elegans 32-kDa beta-galactoside-binding protein is homologous to vertebrate beta-galactoside-binding lectins. cDNA cloning and deduced amino acid sequence. J Biol Chem 267, 15485-15490. Hirabayashi, J., Ubukata, T., and Kasai, K. (1996). Purification and molecular characterization of a novel 16-kDa galectin from the nematode Caenorhabditis elegans. J Biol Chem 271, 2497-2505. Huang, M.T., Mason, J.C., Birdsey, G.M., Amsellem, V., Gerwin, N., Haskard, D.O., Ridley, A.J., and Randi, A.M. (2005). Endothelial intercellular adhesion molecule (ICAM)-2 regulates angiogenesis. Blood 106, 1636-1643. Ide, Y., Miyoshi, E., Nakagawa, T., Gu, J., Tanemura, M., Nishida, T., Ito, T., Yamamoto, H., Kozutsumi, Y., and Taniguchi, N. (2006). Aberrant expression of N-acetylglucosaminyltransferase-IVa and IVb (GnT-IVa and b) in pancreatic cancer. Biochem Biophys Res Commun 341, 478-482. Inaba, N., Hiruma, T., Togayachi, A., Iwasaki, H., Wang, X.H., Furukawa, Y., Sumi, R., Kudo, T., Fujimura, K., Iwai, T., et al. (2003). A novel I-branching beta-1,6-N-acetylglucosaminyltransferase involved in human blood group I antigen expression. Blood 101, 2870-2876. Inoue, S., Lin, S.L., and Inoue, Y. (2000). Chemical analysis of the developmental pattern of polysialylation in chicken brain. Expression of only an extended form of polysialyl chains during embryogenesis and the presence of disialyl residues in both embryonic and adult chicken brains. J Biol Chem 275, 29968-29979. Inoue, S., Lin, S.L., Lee, Y.C., and Inoue, Y. (2001). An ultrasensitive chemical method for polysialic acid analysis. Glycobiology 11, 759-767. Ishida, H., Togayachi, A., Sakai, T., Iwai, T., Hiruma, T., Sato, T., Okubo, R., Inaba, N., Kudo, T., Gotoh, M., et al. (2005). A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer. FEBS Lett 579, 71-78. Jain, A., Tindell, C.A., Laux, I., Hunter, J.B., Curran, J., Galkin, A., Afar, D.E., Aronson, N., Shak, S., Natale, R.B., et al. (2005). Epithelial membrane protein-1 is a biomarker of gefitinib resistance. Proc Natl Acad Sci U S A 102, 11858-11863. Jung, K., Cho, W., and Regnier, F.E. (2009). Glycoproteomics of plasma based on narrow selectivity lectin affinity chromatography. J Proteome Res 8, 643-650. Kang, P., Mechref, Y., Klouckova, I., and Novotny, M.V. (2005). Solid-phase permethylation of glycans for mass spectrometric analysis. Rapid Commun Mass Spectrom 19, 3421-3428. Kanter, J.L., Narayana, S., Ho, P.P., Catz, I., Warren, K.G., Sobel, R.A., Steinman, L., and Robinson, W.H. (2006). Lipid microarrays identify key mediators of autoimmune brain inflammation. Nat Med 12, 138-143. Kasahara, K., Watanabe, Y., Yamamoto, T., and Sanai, Y. (1997). Association of Src family tyrosine kinase Lyn with ganglioside GD3 in rat brain. Possible regulation of Lyn by glycosphingolipid in caveolae-like domains. J Biol Chem 272, 29947-29953. Kelm, S., Schauer, R., Manuguerra, J.C., Gross, H.J., and Crocker, P.R. (1994). Modifications of cell surface sialic acids modulate cell adhesion mediated by sialoadhesin and CD22. Glycoconj J 11, 576-585. Kiang, W.L., Krusius, T., Finne, J., Margolis, R.U., and Margolis, R.K. (1982). Glycoproteins and proteoglycans of the chromaffin granule matrix. J Biol Chem 257, 1651-1659. Kimura, N., Ohmori, K., Miyazaki, K., Izawa, M., Matsuzaki, Y., Yasuda, Y., Takematsu, H., Kozutsumi, Y., Moriyama, A., and Kannagi, R. (2007). Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2. J Biol Chem 282, 32200-32207. Kitajima, K., Kuroyanagi, H., Inoue, S., Ye, J., Troy, F.A., 2nd, and Inoue, Y. (1994). Discovery of a new type of sialidase, 'KDNase,' which specifically hydrolyzes deaminoneuraminyl (3-deoxy-D-glycero-D-galacto-2-nonulosonic acid) but not N-acylneuraminyl linkages. J Biol Chem 269, 21415-21419. Knapp, M.R., Jones, P.P., Black, S.J., Vitetta, E.S., Slavin, S., and Strober, S. (1979a). Characterization of a spontaneous murine B cell leukemia (BCL1). I. Cell surface expression of IgM, IgD, Ia, and FcR. J Immunol 123, 992-999. Knapp, M.R., Severinson-Gronowicz, E., Schroder, J., and Strober, S. (1979b). Characterization of a spontaneous murine B cell leukemia (BCL1). II. Tumor cell proliferation and IgM secretion after stimulation by LPS. J Immunol 123, 1000-1006. Kobata, A. (1988). Structures, function, and transformational changes of the sugar chains of glycohormones. J Cell Biochem 37, 79-90. Kochibe, N., and Furukawa, K. (1980). Purification and properties of a novel fucose-specific hemagglutinin of Aleuria aurantia. Biochemistry 19, 2841-2846. Koenderman, A.H., Koppen, P.L., and Van den Eijnden, D.H. (1987). Biosynthesis of polylactosaminoglycans. Novikoff ascites tumor cells contain two UDP-GlcNAc:beta-galactoside beta 1----6-N-acetylglucosaminyltransferase activities. Eur J Biochem 166, 199-208. Koethe, S., Zander, L., Koster, S., Annan, A., Ebenfelt, A., Spencer, J., and Bemark, M. (2011). Pivotal Advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation. J Leukoc Biol. Kojima, N., Yoshida, Y., Kurosawa, N., Lee, Y.C., and Tsuji, S. (1995). Enzymatic activity of a developmentally regulated member of the sialyltransferase family (STX): evidence for alpha 2,8-sialyltransferase activity toward N-linked oligosaccharides. FEBS Lett 360, 1-4. Kono, M., Yoshida, Y., Kojima, N., and Tsuji, S. (1996). Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3. J Biol Chem 271, 29366-29371. Kornfeld, K., Reitman, M.L., and Kornfeld, R. (1981). The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. J Biol Chem 256, 6633-6640. Kremser, M.E., Przybylo, M., Hoja-Lukowicz, D., Pochec, E., Amoresano, A., Carpentieri, A., Bubka, M., and Litynska, A. (2008). Characterisation of alpha3beta1 and alpha(v)beta3 integrin N-oligosaccharides in metastatic melanoma WM9 and WM239 cell lines. Biochim Biophys Acta 1780, 1421-1431. Lagana, A., Goetz, J.G., Cheung, P., Raz, A., Dennis, J.W., and Nabi, I.R. (2006). Galectin binding to Mgat5-modified N-glycans regulates fibronectin matrix remodeling in tumor cells. Mol Cell Biol 26, 3181-3193. Larsen, M.R., Jensen, S.S., Jakobsen, L.A., and Heegaard, N.H. (2007). Exploring the sialiome using titanium dioxide chromatography and mass spectrometry. Mol Cell Proteomics 6, 1778-1787. Larsen, M.R., Thingholm, T.E., Jensen, O.N., Roepstorff, P., and Jorgensen, T.J. (2005). Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns. Mol Cell Proteomics 4, 873-886. Lau, K.S., Partridge, E.A., Grigorian, A., Silvescu, C.I., Reinhold, V.N., Demetriou, M., and Dennis, J.W. (2007). Complex N-glycan number and degree of branching cooperate to regulate cell proliferation and differentiation. Cell 129, 123-134. Lee, N., Wang, W.C., and Fukuda, M. (1990). Granulocytic differentiation of HL-60 cells is associated with increase of poly-N-acetyllactosamine in Asn-linked oligosaccharides attached to human lysosomal membrane glycoproteins. J Biol Chem 265, 20476-20487. Lee, Y.C., Kim, Y.J., Lee, K.Y., Kim, K.S., Kim, B.U., Kim, H.N., Kim, C.H., and Do, S.I. (1998). Cloning and expression of cDNA for a human Sia alpha 2,3Gal beta 1, 4GlcNA:alpha 2,8-sialyltransferase (hST8Sia III). Arch Biochem Biophys 360, 41-46. Leppanen, A., Stowell, S., Blixt, O., and Cummings, R.D. (2005). Dimeric galectin-1 binds with high affinity to alpha2,3-sialylated and non-sialylated terminal N-acetyllactosamine units on surface-bound extended glycans. J Biol Chem 280, 5549-5562. Leppanen, A., Zhu, Y., Maaheimo, H., Helin, J., Lehtonen, E., and Renkonen, O. (1998). Biosynthesis of branched polylactosaminoglycans. Embryonal carcinoma cells express midchain beta1,6-N-acetylglucosaminyltransferase activity that generates branches to preformed linear backbones. J Biol Chem 273, 17399-17405. Lin, S.L., Inoue, Y., and Inoue, S. (1999). Evaluation of high-performance anion-exchange chromatography with pulsed electrochemical and fluorometric detection for extensive application to the analysisof homologous series of oligo- and polysialic acids in bioactive molecules. Glycobiology 9, 807-814. Lindberg, B., and Lonngren, J. (1978). Methylation analysis of complex carbohydrates: general procedure and application for sequence analysis. Methods Enzymol 50, 3-33. Liu, F.T., Patterson, R.J., and Wang, J.L. (2002). Intracellular functions of galectins. Biochim Biophys Acta 1572, 263-273. Liu, F.T., and Rabinovich, G.A. (2010). Galectins: regulators of acute and chronic inflammation. Ann N Y Acad Sci 1183, 158-182. Liu, H., Kojima, N., Kurosawa, N., and Tsuji, S. (1997). Regulated expression system for GD3 synthase cDNA and induction of differentiation in Neuro2a cells. Glycobiology 7, 1067-1076. Loboda, A.V., Krutchinsky, A.N., Bromirski, M., Ens, W., and Standing, K.G. (2000). A tandem quadrupole/time-of-flight mass spectrometer with a matrix-assisted laser desorption/ionization source: design and performance. Rapid Commun Mass Spectrom 14, 1047-1057. Ma, B.Y., Yoshida, K., Baba, M., Nonaka, M., Matsumoto, S., Kawasaki, N., Asano, S., and Kawasaki, T. (2009). The lectin Jacalin induces human B-lymphocyte apoptosis through glycosylation-dependent interaction with CD45. Immunology 127, 477-488. Mao, X., Luo, Y., Dai, Z., Wang, K., Du, Y., and Lin, B. (2004). Integrated lectin affinity microfluidic chip for glycoform separation. Anal Chem 76, 6941-6947. Merkle, R.K., and Cummings, R.D. (1987). Relationship of the terminal sequences to the length of poly-N-acetyllactosamine chains in asparagine-linked oligosaccharides from the mouse lymphoma cell line BW5147. Immobilized tomato lectin interacts with high affinity with glycopeptides containing long poly-N-acetyllactosamine chains. J Biol Chem 262, 8179-8189. Muramatsu, H., Kusano, T., Sato, M., Oda, Y., Kobori, K., and Muramatsu, T. (2008). Embryonic stem cells deficient in I beta1,6-N-acetylglucosaminyltransferase exhibit reduced expression of embryoglycan and the loss of a Lewis X antigen, 4C9. Glycobiology 18, 242-249. Muramatsu, T. (1988). Developmentally regulated expression of cell surface carbohydrates during mouse embryogenesis. J Cell Biochem 36, 1-14. Nan, B.C., Shao, D.M., Chen, H.L., Huang, Y., Gu, J.X., Zhang, Y.B., and Wu, Z.G. (1998). Alteration of N-acetylglucosaminyltransferases in pancreatic carcinoma. Glycoconj J 15, 1033-1037. Nangia-Makker, P., Honjo, Y., Sarvis, R., Akahani, S., Hogan, V., Pienta, K.J., and Raz, A. (2000). Galectin-3 induces endothelial cell morphogenesis and angiogenesis. Am J Pathol 156, 899-909. Naven, T.J., Harvey, D.J., Brown, J., and Critchley, G. (1997). Fragmentation of complex carbohydrates following ionization by matrix-assisted laser desorption with an instrument fitted with time-lag focusing. Rapid Commun Mass Spectrom 11, 1681-1686. Nemansky, M., Schiphorst, W.E., and Van den Eijnden, D.H. (1995). Branching and elongation with lactosaminoglycan chains of N-linked oligosaccharides result in a shift toward termination with alpha 2-->3-linked rather than with alpha 2-->6-linked sialic acid residues. FEBS Lett 363, 280-284. Neurohr, K.J., Young, N.M., and Mantsch, H.H. (1980). Determination of the carbohydrate-binding properties of peanut agglutinin by ultraviolet difference spectroscopy. J Biol Chem 255, 9205-9209. North, S.J., Hitchen, P.G., Haslam, S.M., and Dell, A. (2009). Mass spectrometry in the analysis of N-linked and O-linked glycans. Curr Opin Struct Biol 19, 498-506. North, S.J., Huang, H.H., Sundaram, S., Jang-Lee, J., Etienne, A.T., Trollope, A., Chalabi, S., Dell, A., Stanley, P., and Haslam, S.M. (2010). Glycomics profiling of Chinese hamster ovary cell glycosylation mutants reveals N-glycans of a novel size and complexity. J Biol Chem 285, 5759-5775. Ohyama, Y., Kasai, K., Nomoto, H., and Inoue, Y. (1985). Frontal affinity chromatography of ovalbumin glycoasparagines on a concanavalin A-sepharose column. A quantitative study of the binding specificity of the lectin. J Biol Chem 260, 6882-6887. Okudaira, T., Hirashima, M., Ishikawa, C., Makishi, S., Tomita, M., Matsuda, T., Kawakami, H., Taira, N., Ohshiro, K., Masuda, M., et al. (2007). A modified version of galectin-9 suppresses cell growth and induces apoptosis of human T-cell leukemia virus type I-infected T-cell lines. Int J Cancer 120, 2251-2261. Olsen, J.V., de Godoy, L.M., Li, G., Macek, B., Mortensen, P., Pesch, R., Makarov, A., Lange, O., Horning, S., and Mann, M. (2005). Parts per million mass accuracy on an Orbitrap mass spectrometer via lock mass injection into a C-trap. Mol Cell Proteomics 4, 2010-2021. Pan, S., Wang, Y., Quinn, J.F., Peskind, E.R., Waichunas, D., Wimberger, J.T., Jin, J., Li, J.G., Zhu, D., Pan, C., et al. (2006). Identification of glycoproteins in human cerebrospinal fluid with a complementary proteomic approach. J Proteome Res 5, 2769-2779. Pang, P.C., Tissot, B., Drobnis, E.Z., Morris, H.R., Dell, A., and Clark, G.F. (2009). Analysis of the human seminal plasma glycome reveals the presence of immunomodulatory carbohydrate functional groups. J Proteome Res 8, 4906-4915. Paulson, J.C., and Rademacher, C. (2009). Glycan terminator. Nat Struct Mol Biol 16, 1121-1122. Peracaula, R., Barrabes, S., Sarrats, A., Rudd, P.M., and de Llorens, R. (2008). Altered glycosylation in tumours focused to cancer diagnosis. Dis Markers 25, 207-218. Peterman, S.M., and Mulholland, J.J. (2006). A novel approach for identification and characterization of glycoproteins using a hybrid linear ion trap/FT-ICR mass spectrometer. J Am Soc Mass Spectrom 17, 168-179. Pierce, M., and Arango, J. (1986). Rous sarcoma virus-transformed baby hamster kidney cells express higher levels of asparagine-linked tri- and tetraantennary glycopeptides containing [GlcNAc-beta (1,6)Man-alpha (1,6)Man] and poly-N-acetyllactosamine sequences than baby hamster kidney cells. J Biol Chem 261, 10772-10777. Piller, F., and Cartron, J.P. (1983). UDP-GlcNAc:Gal beta 1-4Glc(NAc) beta 1-3N-acetylglucosaminyltransferase. Identification and characterization in human serum. J Biol Chem 258, 12293-12299. Piller, F., Cartron, J.P., Maranduba, A., Veyrieres, A., Leroy, Y., and Fournet, B. (1984). Biosynthesis of blood group I antigens. Identification of a UDP-GlcNAc:GlcNAc beta 1-3Gal(-R) beta 1-6(GlcNAc to Gal) N-acetylglucosaminyltransferase in hog gastric mucosa. J Biol Chem 259, 13385-13390. Pitt, J.J., and Gorman, J.J. (1997). Oligosaccharide characterization and quantitation using 1-phenyl-3-methyl-5-pyrazolone derivatization and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem 248, 63-75. Renkonen, O. (2000). Enzymatic in vitro synthesis of I-branches of mammalian polylactosamines: generation of scaffolds for multiple selectin-binding saccharide determinants. Cell Mol Life Sci 57, 1423-1439. Rosenberg, A., ed. (1995). Biology of the sialic acids (New York, Plenum Press, New York). Rossi, B., Espeli, M., Schiff, C., and Gauthier, L. (2006). Clustering of pre-B cell integrins induces galectin-1-dependent pre-B cell receptor relocalization and activation. J Immunol 177, 796-803. Saijonmaa, O., Nyman, T., Hohenthal, U., and Fyhrquist, F. (1991). Endothelin-1 is expressed and released by a human endothelial hybrid cell line (EA.hy 926). Biochem Biophys Res Commun 181, 529-536. Saito, H., Nishikawa, A., Gu, J., Ihara, Y., Soejima, H., Wada, Y., Sekiya, C., Niikawa, N., and Taniguchi, N. (1994). cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+. Biochem Biophys Res Commun 198, 318-327. Saitoh, O., Wang, W.C., Lotan, R., and Fukuda, M. (1992). Differential glycosylation and cell surface expression of lysosomal membrane glycoproteins in sublines of a human colon cancer exhibiting distinct metastatic potentials. J Biol Chem 267, 5700-5711. Sasaki, K., Kurata-Miura, K., Ujita, M., Angata, K., Nakagawa, S., Sekine, S., Nishi, T., and Fukuda, M. (1997). Expression cloning of cDNA encoding a human beta-1,3-N-acetylglucosaminyltransferase that is essential for poly-N-acetyllactosamine synthesis. Proc Natl Acad Sci U S A 94, 14294-14299. Sasaki, K., Kurata, K., Kojima, N., Kurosawa, N., Ohta, S., Hanai, N., Tsuji, S., and Nishi, T. (1994). Expression cloning of a GM3-specific alpha-2,8-sialyltransferase (GD3 synthase). J Biol Chem 269, 15950-15956. Sato, C. (2004). Chain length diversity of sialic acids and its biological significance. Trends Glycosci Glycotechnol 16, 331-344. Sato, C., Fukuoka, H., Ohta, K., | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10103 | - |
dc.description.abstract | 以質譜為基礎的醣質體及醣蛋白質體大多著重在鑑定醣鏈末端乙酰基乳糖胺上的唾液酸及岩藻醣化,對於N型醣鏈上是否為直鏈或是帶有支鏈的多乳糖胺聚醣則較少著墨,且大多只是憑藉著質譜所得的數據來推算其存在和可能的組成為何,因此,本篇論文主要是利用人類內皮細胞、老鼠及人類的B淋巴球為樣品,鑑定並挑戰分析多乳糖胺聚醣的結構特徵及其末端的唾液酸化和硫酸化修飾。
首先,以人類內皮細胞:EA.hy926和HUVEC為起始原料,利用介質輔助雷射脫附法和電噴灑離子化方法在MS及MS/MS的階段來仔細地探討多乳糖胺聚醣結構,同時並搭配endo-β-galactosidase酵素及Smith降解反應來鑑定其是否為分支醣鏈及長鏈延伸的起始位置。和HUVEC相比,EA.hy926細胞的N型醣鏈帶有較少的唾液酸化和岩藻醣化,但多乳糖胺聚醣的長度較長且具有分支醣鏈,其延伸點並不侷限在目前已被證實具有相當重要生物意義的甘露醣6號碳上。拓展至醣質體方面的研究上,則利用Lycopersicon esculentum凝集素分別在醣、醣蛋白及醣胜肽的階段做純化,較為專一的兩步驟純化方法讓我們得知了至少有40個以上的醣蛋白候補者可能帶有乳糖胺聚醣。 小鼠B細胞株—BCL1上N型醣鏈的修飾主要為核心岩藻醣化,末端α鏈結半乳糖及唾液酸(Neu5Gc)化,且為非分支的多乳糖胺聚醣;相反的是,其O型醣鏈主要為簡單的core 1結構,帶有單唾液酸或雙唾液酸修飾。利用唾液酸酶、 endo-β-galactosidase、MS/MS及化學分析方法可知,雙唾液酸主要靠α2-8鏈結,並同時存在於具有多乳糖胺聚醣延伸或非延伸的N型醣鏈上。以螢光標記唾液酸並搭配高效液相層析儀顯示雙唾液酸結構的含量在N型醣鏈和O型醣鏈是相當的,且CD45為其攜帶者之ㄧ。透過小鼠α2,8-sialyltransferase VI(ST8sia VI)基因抑制實驗可知,此酵素同時參與N型醣鏈和O型醣鏈上雙唾液酸的生合成,且ST8Sia VI和雙唾液酸結構的表現量皆會隨著B細胞分化而增加。 有趣的是,儘管其生理功能目前還不是很清楚,BCL1的N型醣鏈末端的單唾液酸、雙唾液酸化乙酰基乳糖胺,及N型醣鏈核心結構皆可被硫酸化修飾。另外,在人類活化的B淋巴球上也鑑定到了α2,6-sialyated 6-sulfo-LacNAc結構,單和α2-6唾液酸化乙酰基乳糖胺相比,其為目前已知CD22更好的配體,這些B細胞上多乳糖胺聚醣鏈附加修飾的鑑定,使得Galectin和Siglec對B細胞分化的調控可以更為複雜精緻。總而言之,質譜分析技術的發展和進步,對於我們詳細地鑑定多乳糖胺聚醣結構來講,為一個相當重要的基礎,有助於我們對醣生物學及其它生理功能更近一步的了解。 | zh_TW |
dc.description.abstract | Most mass spectrometry (MS)-based glycomic and glycoproteomic analyses focus on identifying changes in terminal glyco-epitopes represented by sialylation and fucosylation at specific positions of the terminal N-acetyllactosamine units. Much less attention was accorded to the underlying linear or branched poly-N-acetyllactosamine (polyLacNAc) extension from the N-glycan trimannosyl core other than a simple inference of its presence due to mass data and hence glycosyl compositional assignment. To advance the frontiers of glycomics, this thesis work aims primarily to address the analytical challenges in structural characterization of polylactosaminoglycans and associated terminal modifications such as sialylation and sulfation decorating the human endothelial cells, mouse and human B cells.
Using the human endothelial cells, EA.hy926 and HUVEC, as starting materials, we have systematically investigated the MALDI- and ESI-MS-based methodologies for probing the structural details of polyLacNAc at both MS and MS/MS levels in conjunction with the use of endo-β-galactosidase and Smith degradation to identify branching motifs and initiation sites. N-glycans in EA.hy926 were found to be less sialylated and fucosylated but more extended and branched than those of HUVEC, thus demonstrating a fundamental glycomic difference. For EA.hy926, its polyLacNAc chains were shown to be not restricted to extending from a specific antenna including the biologically important 6-arm position. Extending to glycoproteomics, the Lycopersicon esculentum lectin based enrichment strategy was optimized at glycan, glycoprotein, and glycopeptide levels, leading to identification of over 40 protein carriers utilizing a two-step enrichment workflow. For mouse B cells, the N-glycans of a B lymphoma cell line, BCL1, were found to be mostly core-fucosylated, capped with α-Gal or Neu5Gc sialic acid, and carry non-branched polyLacNAcs. In contrast, its O-glycans were based on simple core 1 structures, mono- or disialylated on both arms. Sialidase digestion, in conjunction with further MS/MS and chemical analyses, established the identity of the terminal disialyl motif as Neu5Gcα2-8Neu5Gc-, which was shown by endo-β-galatosidase digestion to be additionally present on both polyLacNAc extended and non-extended N-glycans. Fluorescent-labeling of released sialic acids coupled with fluorometric high performance liquid chromatography analysis revealed that the amount of the disialyl motif was comparable for both N- and O-glycans, and CD45 is one of the protein carriers. Gene knockdown studies provided positive correlation indicative of mouse α2,8-sialyltransferase VI (ST8sia VI) being involved in the biosynthesis of disialic acid on both N- and O-glycans. Importantly, both the expression level of ST8sia VI and the total amount of disialic acids increase during B cell differentiation. Interestingly, sulfation was additionally found on the terminal mono- and disialylated LacNAc of the polyLAcNAc chains, as well as on the LacNAc proximal to the trimannosyl core in BCL1 although its biological relevance is at present unclear. On the other hand, similar analysis led to identification of α2,6-sialylated 6-sulfo-LacNAc epitope on both the N- and O-glycans of activated human B cells, which is known to constitute a better ligand than the non-sulfated α2,6-sialylated LacNAc for human CD22. These additional modifications of polyLacNAcs apparently complicate the simplistic interpretation of the modulating roles of galectins and Siglecs in the B cell differentiation model. The development of enabling analytical techniques sensitive enough to identify and characterize the fine structural details of the underlying polyLAcNAc is an important step towards a better understanding of the glycobiology of this and many other physiological processes. | en |
dc.description.provenance | Made available in DSpace on 2021-05-20T21:02:09Z (GMT). No. of bitstreams: 1 ntu-100-D94b46003-1.pdf: 5057379 bytes, checksum: d9927be13f79ee9a7db12fec55c09bd5 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | CHAPTER I INTRODUCTION 1
1.1 Glycosylation, Glycomics and Glycoproteomics 1 1.2 Strategies and approaches for glycosylation analysis 3 1.2.1 Mass spectrometry for glycosylation analysis 6 1.2.2 MALDI-MS/MS sequencing of glycans 8 1.2.3 Glycopeptide enrichment for Glycoproteomics 10 1.3 Structural and Functional Implications of poly-N-acetyllactosamine 11 1.3.1 Biosynthesis of polyLacNAc and its potential biological function 12 1.3.2 Mapping the occurrence of polyLacNAc 13 1.4 Occurrence of terminal disialylation 15 1.4.1 Sialyltransferases responsible for the biosynthesis of disialic acid 16 1.4.2 Detection of disialic acids 17 1.5 Specific aims 18 CHAPTER II MATERIAL AND METHODS 20 2.1 Cells and culture conditions 20 2.2 Release of N-glycans and O-glycans from whole cell lysate 21 2.3 Enrichment of polyLacNAc-carrying glycopeptides for glycoproteomic studies 21 2.3.1 First step glycoprotein enrichment from total lysates 21 2.3.2 Isolation of membrane fraction for one step glycopeptide enrichment 22 2.3.3 Glycopeptide enrichment 23 2.4 Immunoprecipitation of CD45 from BCL1 for chemical analysis 23 2.5 Glycosidase digestions and subsequent clean-up or fractionation 24 2.6 Mild periodate oxidation and Smith degradation 25 2.7 Permethylation and microscale fractionation 25 2.8 Analysis of DMB-sialic acid derivatives 27 2.9 GC-MS methylation analysis 28 2.10 MALDI-MS and MS/MS analysis 28 2.11 NanoESI-MS and Total ion mapping analysis 29 2.12 LC-MS/MS shotgun proteomics analysis 29 2.13 RNA isolation, RT-Quantitative (Q)PCR, and knockdown of ST8sia VI 30 CHAPTER III RESULTS 32 PART I. POLYLACNACS ON ENDOTHELIAL CELLS 32 3.1 Direct glycomic profiling of glycans with polyLacNAc 32 3.1.1 MALDI-based MS analysis of HUVEC and EAhy926 32 3.1.2 nanoESI-based Total ion mapping (TIM) at MS2 level 33 3.2 Mapping the fine structural details of polyLacNAc 35 3.2.1 Applications of endo-β-galactosidase digestion 35 3.2.2 Applications of Smith degradation after further size fractionation 37 3.3 Enrichment of polyLacNAc-carrying glycopeptides for glycoproteomics 39 3.3.1 Specificity of tomato lectin at the glycan level 39 3.3.2 One-step and two-step enrichment of glycopeptides with polyLacNAc 40 PART II. POLYLACNACS ON B CELLS 42 3.4 Identification of polyLacNAcs and terminal disialyl motif on BCL1 42 3.4.1 High abundance of terminal disialyl motif on the O-glycans 43 3.4.2 DMB-sialic acid analysis on the total O- and N-glycan pools and CD45 44 3.4.3 Terminal Disialyl motif on both polyLacNAc extended and non-extended N-glycans 45 3.5 Sialyltransferases responsible for the biosynthesis of disialic acids 47 3.5.1 ST8Sia in BCL1 47 3.5.2 ST8Sia VI expression and disialic acid content during B cell differentiation 47 3.6. Sulfated glycans in B cells 48 3.6.1 Sulfated N-glycans in BCL1 48 3.6.2 Sulfated N- and O-glycans in activated human B cells 48 CHAPTER IV DISCUSSION 84 4.1 Technical Accomplishments 84 4.2 Outstanding Technical Limitations and Prospects 85 4.2.1 Application of endo-β-galactosidase to quantify the length of polyLacNAcs 85 4.2.2 The glycoproteomics of polyLacNAcs 86 4.3 The biological implications of polyLacNAc 88 4.4 Disialylation and sulfation on B lymphocytes 90 CHAPTER V REFERENCES 94 | |
dc.language.iso | en | |
dc.title | 內皮細胞及B細胞多乳糖胺聚醣與雙唾液酸醣質體的有效鑑定 | zh_TW |
dc.title | Glycomic mapping of polylactosaminoglycans, terminal disialyl and sialyl sulfo N-acetyllactosamine motifs on mammalian cells | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 劉扶東(Fu-Tong Liu),林俊宏(Chun-Hung Lin),林國儀(Kuo-I Lin),陳玉如(Yu-Ju Chen) | |
dc.subject.keyword | 質譜儀,多乳糖胺聚醣,雙唾液酸,硫酸化, | zh_TW |
dc.subject.keyword | Mass spectrometry,polylactosaminoglycans,disialic aicd,sulfation, | en |
dc.relation.page | 108 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2011-07-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf | 4.94 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。