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標題: | 轉殖小枯草桿菌纖維素酶對於雲杉紙漿吸附、降解與磨漿促進效能 Effect of Recombinant Paenibacillus Cellulase on Adsorption, Degradation and Refining Assistance of Spruce Pulps |
作者: | Wan-Jyun Liao 廖婉均 |
指導教授: | 柯淳涵 |
關鍵字: | 酵素吸附,酵素水解,聚合度,磨漿,微纖維分析, Adsorption,Hydrolysis,Degree of Polymer,Refined,Morfi, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 本研究探討Paenibacillus 所生產之纖維素酶於生質能與造紙生產的作用,以此特定纖維素酶與雲杉紙漿纖維素反應,研究其吸附、水解效能及和木質素 (lignin)、酵母添加物 (yeast extract) 的交互作用,以及探討酵素處理對於磨漿的纖維性質變化。
研究顯示,與水解作用無關的雜蛋白質會優先吸附於紙漿纖維外部的木質素,而外加的酵母抽出物會增強雜蛋白吸附於木質素現象。纖維素酶水解系統會受到以下複雜因子影響:與水解作用無關的雜蛋白質、紙漿木質素和纖維素含量。實驗分別加入纖維素酶劑量5 IU及50 IU與不同木質素漿樣反應,相對於原漿聚合度,其高木質素 (12.4%) 漿樣之LODP僅降解至82.3 % 與 62.1 % 的程度,而低木質素 (1.2%) 漿樣之LODP則降至69.7 % 與60.7 %。此外,添加酵母抽出物,可使雜蛋白質與木質素作用而讓水解蛋白正確吸附在目標物纖維素,因而增加了水解酶的效率,改變水解機制。 另一方面,纖維素酶的前處理將改變磨漿紙漿性質。酵素前處理,會使磨漿纖維表面產生變化,增加纖維對水的親和力,如紙漿濾水性質及纖維水合程度;此外,以微纖維分析儀 (Morfi) 觀察微纖維尺度的紙漿纖維,比較無酵素處理的控制組與有酵素前處理組別,可知纖維素酶前處理會造成較寬的纖維、扭結角度 (kink angle) 較小、較少漿絮 (small fines)、更多破裂纖維和增加微纖維長度的比例。經酵素處理後,紙力也產生變化。透過掃描式顯微鏡 (SEM) 可發現纖維素酶前處理會增加磨漿纖維表面粗糙度 (coarseness)。 This article focused on two sides of action recombinant Paenibacillus cellulase on spruce kraft pulps. One is about the study of interaction of cellulase and pulp on different conditions among four kinds of lignin contents and adding yeast extract. Another subject would discuss the refined fiber properties with the cellulase-pretreatment. We observed that irrelevant proteins preferentially adsorbed on pulp surface with higher lignin contents than cellulase, and the addition of yeast extract strengthened the above trend. The cellulase hydrolysis rates were influenced by various factors on this system: the presence of irrelevant proteins, pulp lignin, and cellulose contents. The high lignin sample (12.4%) with cellulase dosages of 5 IU and 50 IU would degrade DP of 82.3 % and 62.1 %; and the low lignin sample with same enzyme treated would degrade DP of to LODP to 69.7% and 60.7% of original LODP values. Addition of yeast extract further promoted preferential adsorption of irrelevant proteins, and increased the hydrolysis rates by cellulase and altered the mode of hydrolysis. On the other hand, the pretreatment of cellulase through refined would raise the pulp properties. The enzyme modified the interfacial properties of fibers, increasing the affinity for water, such as pulp drainability and fiber hydration. Moreover, the enzymatic pretreatment also changed the distribution of microfiber (Morfi), causing wider fiber, less kink angle and small fines, more broken ends and rate in length of microfiber. The strength properties of the pulp had change, and the SEM also showed that the fiber would increase the coarseness with cellulase treated. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10007 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 森林環境暨資源學系 |
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