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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.author | Chia-Ying Chu | en |
dc.contributor.author | 朱家瑩 | zh_TW |
dc.date.accessioned | 2021-07-01T08:20:01Z | - |
dc.date.available | 2021-07-01T08:20:01Z | - |
dc.date.issued | 1997 | |
dc.identifier.citation | Akimenko, M.-A., Johnson, S. L., Westerfield, M., and Ekker, M. (1995) Differential induction of four mxs homeobox genes during fin development and regeneration in zebrafish. Development 121, 347-357. Alam, J. and Cook, J. L. (1990) Reporter genes: Application to the study of mammalian gene transcription. Anal. Biochem. 188, 245-254. Amsterdam, A., Lin, S., and Hopkins, N. (1995) The Aequorea victoria green fluorescent protein can be used as a reporter in live zebrafish embryos. Dev. Biol. 171, 123-129. Amsterdam, A., Lin, S., Moss, L. G. and Hopkins, N. (1996) Requirements for green fluorescent protein detection in transgenic zebrafish embryos. Gene 173, 99-103. Bayer, T. A., and Campos-ortega, A. (1992) A transgene containing lacZ is expressed in primary sensory neurons in zebrafish. Develpoment 115, 421-426. Caldovic, L. and Hackeet, P. B. (1995) Development of position-independent expression vectors and their transfer into transgenic fish. Mol. Mar. Biol. Biotechnol. 4, 51-61. Chalfie, M. (1995) Green fluorescent protein. Photochem. Photobiol. 62, 651-656. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994) Green fluorecent protein as a marker for gene expression. Science 263, 802-806. Chang, M. S., Chang, G. D., Leu, J. H., Huang, F. L., Chou, C. K., Huang, C. J., and Lo, T. B. (1996) Expression, characterization and genomic structure of carp JAK1 kinase gene. DNA. Cell. Biol. 15,827-844. Chang, M. S., Leu, J. H., Yao, C. W., Huang, F. L., and Huand, C. J. (1997) Genomic structure and promoter region of c-fos gene of round-spotted pufferfish, Tetraodon fluviatilis. Zool. Stud. 36, page unknowen. Chen, C., and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745-2752. Chen, E. Y. and Seeburg, P. H. (1985) Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4, 165-170. Chen, S.N. and Kou, G. H. (1986). Establishment, characterization and application of 14 cell lines from warm-water fish. In Invertebrate and Fish Tissue Culture. E. Kurstak and Y. Kuroda, eds. (Japan Societies Press, Springer-Verlag) pp. 218-227. Cilp, P., N?sslein-volhard, C., and Hopkins, N. (1991). High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc. Natl. Acad. Sci. USA 88, 7953-7957. Cody, C. W., Prasher, D. C., Westler, W. M., Prendergast, F. G, and Ward, W. W. (1993) Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry 32, 1212-1218. Conway, G., Margoliath, A., Wong-Madden, S., Roberts, R. J., and Gilbert, W. Jakl kinase is required for cell migrations and anterior specification in zebrafish embryos. Proc. Natl. Acad. Sci. USA 94, 3082-3087. Cubitt, A. B., Heim, R., Adams, S. R., Boyd, A. E., Gross, L. A., and Tsien, R. Y. (1995). Understanding, improving and using green fluorescent proteins. Trands Biochem. Sci. 20, 448-455. Cui, c., Wani, M. A., Wight, D., Kopchick, J. and Stambrool, P. J. (1994) Reporter genes in transgenic mice. Transgenic Res. 3, 182-194. Culp, P., Nusslein-Volhard, C., and Hopkins, N. (1991) High-frequency germ-line transmission of plasmid DNA sequences injected into fertilized zebrafish eggs. Proc. Natl. Acad. Sci. USA 88, 7953-7957. de Wet, J. R., Wood, K. V., Helinski, D. R., and DeLuca, M. (1985) Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc. Natl. Acad. Sci. USA 82, 7870-7873. DiMattia, G. E., Rhodes, S. J., Krones, A., Carri?re, C., O'Connell, S., Kalla, K., Arias, C., Sawchenoko, P., and Rosenfeld, M. G. (1997) The PIT-1 gene is regulated by distinct early and late pituitwry-specific enhancers. Dev. Biol. 182, 180-190. Driever, W., Stemple, D., Schier, A., and Solnica-Krezel, L. (1994) Zebrafish: genetic tools for studying vetebrate development. Trends Genet. 10, 152-159. Durbec, P., Marcos-Guti?rrez, C. V., Kilkenny, C., Grigoriou, M., Wartiowaara, K., Suvanto, P., Smith, D., Ponder, B., Costantini, F., and Saarma, M., (1996).GDNF signaling through the RET receptor tyrosine kinase. Nature 381, 789-793 Edlund, T., Walker, M. D., Barr, P. J., and Rutter, W. J. (1985) Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements. Science 230, 912-916 Eisen, J. S. (1996) Zebrafish make a big splash. Cell 87, 967-977. Etkin, L. D., and Pearman B. (1987) Distribution, expression and germ line transmission of exogenous DNA sequences following microinjection into Xenopus laevis eggs. Development 99, 15-23. Fowler, A. V., and zabin, I. (1983) Purification, structure, and properties of hybrid beta-galactosidase proteins. J. Biol. Chem. 258, 14354-14358 Friedenreich, H. and Schartl, M. (1990) Transient expression directed by homologous and heterologous promoter and enhancer sequences in fish cells. Nucleic Acids Res. 18, 3299-3305. Gerdes, H.-H. and Kaether, C. (1996). Green fluorescent protein: application in cell biology. FEBS lett. 389, 44-47. Gould, S. J., and Subramani, S. (1988) Firefly luciferase as a tool in molecular and cell biology. Anal. Biochem. 175, 5-13. Gross, G. and Hauser, H. (1995). Heterologous expression as a tool for gene identification and analysis. J. Biotech. 41, 91-110. Hastings, J. W. (1996) Chemistries and color of bioluminescent reactions: a review. Gene 173, 5-11. Heim, R. and Tsien, R. Y. (1996). Engineering green fluorescent protein for improves brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6, 178-182. Herbomel, P., Bourachot, B., nad Yaniv, M. (1984). Two distinct enhancers with different cell specificities coexist in the regulatory region of Polyoma. Cell 39, 653-662. Ihssen, P. E., McKay, L. R., McMillan, I. and Phillips, R. B. (1990) Ploidy manipulation and gynogenesis in fished: cytogenetic and fisheries applications. Trans. Am. Fish. Soc. 119, 698-717. Inoue, K., Yamashita, S., Akita, N., Mitsuboshi, T., Nagahisa, E., Shiba, T. and Fujita, T. (1990) Electroporation as a new technique for producing transgenic fish. Cell. Differ. Develop. 29, 123-128. Inouye, S. and Tsuji, F. I. (1994) Aquorea green fluorescent protein expression of the gene and fluorescence characteristics of the recombinant protein. FEBS lett. 341, 277-280. Iyrngar, A., and Maclean, N. (1995) Transgene concatemerization and expression in rainbow trout (Oncoyhynchus mykiss). Mol. Mar. Biol. Biotechnol. 4, 248-254. Jing, S., Wen, D., Yu, Y., Holst, P.L., Luo, Y., Fang, M., Tamir, R., Antonio, L., Hu, Z., Cupples, R., Louis, J.C., Hu, S., Altrock, B.W., Fox, G.M. (1996) GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-α, a novel receptor for GDNF. Cell 85, 1113-1124 Kain, S. R., Adams, M., Kondepudi, A., Yang, T.-T., Ward, W. W., and Kitts, P. (1995). Green fluorescent protein as a reporter of gene expression and protein localization. Bio Tech. 19, 650-655. Kimmel, C. B., Warga, R. M., and Schilling, T.F. (1990) Origin and organization of the zebrafish fate map. Development 108, 581-594 Kindy, M. S., and Verma, I. M. (1990) developmental expression of the Xenopus laevis fos protooncogene. Cell Growth Differ. 1, 27-37. Komarova, E. A., Chernov, M. V., Franks, R., Wang, K., Armin, G., Zelnick, A. R., Chin, D. M., Bacus, S. S., Stark, G. R., and Gudkov, A. V. (1997) Transfenic mice with p53- responsive lacZ: p53 activity varies dramativally during nomal development and determines rafiation anf drug sestivity in vivo. EMBO 16, 1391-1400. Leu, J. H., Chang, M. S., Yao, C. W., Chou, C. K., Chen, S.T., and Huang, C. J. (1997) Genomic organization and characterization of the promoter region of the round-spotted pufferfish (Tetraodon fluviatilis) JAK1 kinase gene. Submitted to B. B. A. Liao, Y., Yi, T., Hoit, B. D., Walsh, R. A., and Karnik, S. S. (1997) Selective reporter expression in mast cells using a chymase. J. Biol. Chem. 272, 2969-2976. Lin, S., Ttang, S., and Hopkins, N. (1994) lacZ Expression in germline transgenic zebrafish cen be detected lin living embryos. Dev. Biol. 161, 77-83. Marcros-Guti?erez, C., Wilson, S. W., Holder, N., and Pachnis, V. (1997) The zebrafish homologue of the ret receptor and its pattern of expression during embryogenesis. Oncogene 14, 879-889. McFarlane, M., and Wilson, J. B. (1996) A model for the mechanism of precise integration of a microinjected transgene. Transgenic Res. 5, 171-177. Moss, J. B., Price, A. L., Raz, E. Driever, W. and Rosenthal, N. (1996). Green fluorescent protein marks skeletal muscle in murine cell lines and zebrafish. Gene, 173, 89-98. Mullis, K. B. and Faloona, F. A. (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods. Enzymed. 155, 335-350. Pachnis, V., Mankoo, B., and Constantini, F. (1993) Expression of the c-ret proto-oncogene during mouse embryogenesis. Development 119, 1005-1017. Peters, K., Rao, P. S., Bell, B. S., and Kindman, L. A. (1995) Green fluorescent fusion proteins: Powerful tools for monitoring protein expression in live zebrafish embryos. Dev. Biol. 171, 252-257. Pines, J. (1995) GFP in mammalian cells. Trends Genet 11, 326-327. Postlethwait, J. H. and Talbot, W. S. (1997) Zebrafish genomics: from mutants to genes. Trands Genet. 13, 183-190. Reinhard, E., Nedivi, E., Wegner, J., Pate Skene, J.H., and Westerfield, M. (1994) Neural selectivr activation and temporal regulation of a mammalian GAP-43 promoter in zebrafish. Development 120, 1767-1775. Rinder, H., Bayer, T. A., Gertzen, E-M., and Hoffmann, W. (1992) Molecular analysis of the ependymin gene and functional test of its promoter region by transient expression in Brachydanio rerio. DNA. Cell. Biol. 11, 425-432. Rizzuto, R., Brini, M., Pizzo, P., Murgia, M. and Pozzan, T. (1995). Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr. Biol. 5, 635-642. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular cloning: a laboratory Manual, 2nd ed., p. 16.8 Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. Sanchez, M.P., Silos-Santiago, I., Frisen, J., He, B., Lira, S.A., Barbacid, M., (1996). Renal agenesis and the absence of enteric neurons in mice lacking GDNF. Nature 382, 70-73 (1996) Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chain-terminating inhibitor. Proc. Natl. Acad. Sci. USA 74, 5463-5467. Sanger, F., Nicklen, S., and Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467. Schier, A. F. (1997) Genetics of neural development in zebrafish. Curr. Opin. Neurobiol. 7, 119-126. Seed, B., and Sheen, J.-Y. (1988) A simple phase-extraction assay for chloramphenicol acyltransferase activity. Gene 67, 271-277. Sepulveda, A. R., Huang, S. L., Lebovitz, R. M., and Lieberman, M. W. (1997) A 346-base pair region of the mouse γ-glutamyl transpeptidease type II promoter contains sufficient cis-acting elements for kidney-restricted experssion in transgenic mice. J. Biol. Chem. 272, 11959-11967. Shimizu, M., Ichikawa, E., Inoue, U., Nakamura, T., Nakajima, N., Nojima. H., Okayama, H. and Oda, K. (1995). The G1/S boundary-specific enhancer of the rat cdc2 promoter. Mol. Cell. Biol. 15, 2882-2892. Stuart, G. W., Vielkind, J. R., McMurry, J. V., and Westerfield, M. (1990) Stable lines of transgenic zebrafish exhibit reproducible pattern of transgenic expression. Development 109, 577-584. Stuart, G., McMurry, J. V., and Westerfield, M. (1988) Replication, integration and stable germ-line transmission of foreign sequences injected into early zebrafish embryos. Development 103, 403-412. Subramani, S., and DeLuca, M. (1988) in Genetic engineering (Setlow, J. K., and Hollender, A., eds), Vol. 10, pp.75-89, Plenum, New york. Terasaki, M., Jaffe, L. A., Hunnicutt, G. R., and Hammer, J. A. (1996) Structural change of the endoplasmic reticulum during fertilization: evidance for loss of membrane continuity using the green flourescent protein. Dev. Biol. 179, 320-328. Tommasi, S., and Pfeifer, G. P. (1995) In vivo structure of the human cdc2 promoter: relesase of a p130-E2F-4 complex from sequences immediately upstream of the transcription initiation site coincides with induction of cdc2 expression. Mol. Cell. Biol. 15. 6901-6913. Treanor, J.J., Goodman, L., de Sauvage, F., Stone, D.M., Poulsen, K.T., Beck, C.D., Gray, C., Armanini, M.P., Pollock, R.A. Hefti, F., Phillips, H.S., Goddard, A., Moore, M.W., Buj-Bello, A., Davies, A.M., Asai, N., Takahashi, M., Vandlen, R., Henderson, C.E., Rosenthal, A., (1996). Characterization of a multicomponent receptor for GDNF. Natrue 382, 80-83. Trupp, M., Arenas, E., Fainzilber, M., Nilsson, A.S., Sieber, B.A., Grigoriou, M., Kilkenny, C., Salazar-Grueso, E., Pachnis, V., Arumae, U. (1996) Functional receptor for GDNF encoded by the c-ret proto-oncogene. Nature 381, 785-788 (1996) Westerfield, M., Wegner, J., Jegalian, B. G., DeRobertis, E. M., and Puschel, A. W. (1992) Specific activation of mammalian Hox promoters in mosaic transgenic zebrafish. Genes. Dev. 6, 591-598. Winkler, C., Vielkind, J. R., and Schartl, M. (1991) Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes). Mol. Gen. Genet. 226, 129-140. Wynshaw-Boris, A., Short, J. M., Hanson, R. W. (1987) Regulation of gene transcription by multiple hormones: organization of regulatory elements. Prog Nucleic Acid Res Mol Biol 34, 59-87 (1987) Zernicka-Goetz, M., Pines, J., Ryan, K., Siemering, K. R., Haseloff, J., Evans, M. J. and Gurdon, J. B. (1996) An indelible lineage marker for Xenopus using a mutanted green fluorescent protein. Development 122, 3719-3724. 蕭培文(1994)鯉魚cdc2 cDNA之選殖及其cdk基因結構之研究.國立台灣大學生化科學研究所碩士論文 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/76297 | - |
dc.description.abstract | 為了研究細胞株及胚胎中的啟動子活性和調控機制,我們構築數種包含不同基因啟動子區域的檢測基因表現載體。使用的檢測基因為β-半乳糖?以及綠螢光蛋白(GFP)。啟動子區域則分別來自鯉魚(Cyprinus carpio)的JAK1、cdc2基因,和圓斑河魨(Tetraodon fluviatilis)的JAK1、fos、RET基因。在細胞株中的活性分析顯示,河魨JAK1基因啟動子的活性僅為正對照組CMV啟動子活性的7%;河魨fos基因啟動子較強,為CMV啟動子的15%。若以顯微注射法將各表現質體送入斑馬魚(Danio rerio)受精卵中進行活性分析,我們發現河魨JAK1啟動子有較高的表現活性,約達CMV啟動子的80%,而河魨fos基因啟動子僅為30%。在胚胎中由於具有不同的細胞種類,能提供不同的基因調控條件,因此與細胞株中的in vitro結果有所不同。分析各基因啟動子在胚胎中的作用部位,發現河魨JAK1、河魨fos、和鯉魚JAK1基因啟動子表現部位多集中於軀幹中段;河魨RET較集中於頭部;而鯉魚cdc2基因啟動子則有全身性的表現。統計不同啟動子表現的細胞種類,鯉魚cdc2基因啟動子及河魨RET基因啟動子多為表皮和頭部區域,河魨JAK1、河魨fos、鯉魚JAK1等基因啟動子則多出現於肌肉和脊索中,顯示部份的基因啟動子可能具有組織專一性的表現能力。此外,以不同長度的鯉魚cdc2基因啟動子進行活性分析,我們發現可能存在一個轉錄增強序列,該序列(5'-AAATTAACAAA-3')與已知老鼠cdc2基因的轉錄增強序列(5'-AAGTTACAAA-3')有很高的相似性。由於檢視β-半乳糖?活性時,必須犧牲胚胎進行固定染色,因此另外選用GFP作為檢測基因,追蹤觀察啟動子在活體中的表現活性。我們所構築的鯉魚JAK1啟動子GFP表現質體,能成功地表現於斑馬魚胚胎及幼魚體中,而在肌肉、脊索等部位放出綠色螢光。利用本論文在斑馬魚胚胎中偵測啟動子活性所建立的in vivo分析方式,將可應用於基因轉殖魚類的研究上,達到攜帶基因於特殊部位表現,或是全身性強而有效表現等目的。 | zh_TW |
dc.description.abstract | In order to study the gene regulation in cell lines and embryos, several expression vectors containing different heterologous promoters as well as lacZ reporter gene were constructed. The promoter regions were obtained from a variety of fish genes including common carp (Cyprinus carpio) JAK1 and cdc2 genes as well as the round-spotted pufferfish (Tetraodon fluviatilis) JAK1, RET and c-fos genes. The results of promoter assay were different in cell lines and in embryos. When transfected into carp CF cell line, pf-fos promoter displayed strong lacZ activity, about 15% of that of pCMV β, While pf-JAK1 promoter had only 7% of that of pCMV β. When these constructs were injected into zebrafish (Danio rerio) embryos at one-cell-stage, the pf-JAK1 promoter displayed stronger expression activity than that of pf-fos promoter. Analyzing the expression pattern of 24 -h (hours after fertilization at 28℃) embryos, the β-gal-expressed cells of pf-JAK1 and c-JAK1 promoters were predominantly located in notochord and muscle in the trunk segments of embryos, whereas those of c-cdc2 promoter were located ubiquitously. The majority of expressing cells of pf-RET promoter was located in the head and skin region. We also performed deletion analysis of c-cdc2 promoter and a positive regulation region between -211 and -146 was defined. A sequence in the region (5'-AAATTAACAAA-3') has high similarity with rat cdc2 enhancer element (-276AAGTTACAAA-267). With the goal of detecting gene expression in the embryo at different developmental stages, the green fluorescent protein (GFP) from the jellyfish (Aequorea victoria) was chosen as reporter gene. GFP fluorescence can be observed non-invasively in living cells without any substrate or cofactor. A GFP expression vector driven by c-JAK1 promoter was constructed and microinjected into zebrafish embryos. At 44-h and 72-h after injected, the green fluorescence was visible in muscle and notochord. Our data indicated that the transient expression system, by means of transgenic zebrafish, could be used as rapid analysis of promoter activity and gene regulation in vivo. Utilizing this system, we found that some promoters were expressed ubiquitously whereas other promoters were expressed at restricted area. Thus, analysis of promoter activity would be useful for further studies. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:20:01Z (GMT). No. of bitstreams: 0 Previous issue date: 1997 | en |
dc.description.tableofcontents | 目錄 i 中文摘要 iii 英文摘要 v 第一章 前言 1 基因啟動子的研究 1 檢測基因表現載體 2 基因轉殖動物及活體(in vivo)檢測啟動子活性 5 基因轉殖魚類的研究 6 本論文的研究主題 7 第二章 材料與方法 9 1.材料 9 2.魚類各基因之選殖 9 3.β-半乳糖?(β-galactosidase)基因表現載體之構築 10 3.1 聚合?連鎖反應(polymerase chain reaction, PCR) 10 3.2 電泳純析(Electroelution) 11 3.3 DNA接合反應(DNA ligation) 11 3.4 轉形細胞(Competent cells)的製備 11 3.5 細菌的轉形作用(transformation) 12 3.6 質體DNA的快速製備 12 3.7 核酸序列分析 13 4.魚類各種基因啟動子在鯉魚細胞株中的活性分析 13 4.1 魚類細胞培養 13 4.2 小量純化質體DNA 13 4.3 基因轉染(DNA transfection) 14 4.4 收集細胞萃取液 14 4.5 β-半乳糖?活性試驗 14 4.6 氯黴素乙醯轉移?試驗(CAT assay) 15 4.7 X-gal呈色反應 15 5.各種魚類基因啟動子在斑馬魚胚胎中的活性分析 16 5.1 純化質體DNA 16 5.2 顯微注射β-半乳糖?基因表現之分析 16 6.以綠螢光蛋白基因(Green Fluorescent Protein, GFP)做為偵測基因(reporter gene)研究carp-JAK1基因啟動子長期的活性 17 6.1 綠螢光蛋白表現載體的構築 17 6.2 鯉魚細胞株中的活性檢視 17 6.3 斑馬魚中的活性檢視 17 第三章 結果 18 構築轉殖基因β-半乳糖?表現載體 18 各基因啟動子在細胞株中的表現作用 18 各基因啟動子在斑馬魚胚胎中的作用 18 各基因啟動子在細胞株及斑馬魚胚胎的活性比較 22 胚胎中各基因啟動子作用的分佈情形 22 鯉魚cdc2基因啟動子不同區域片段在細胞株及斑馬魚胚胎中的活性比較 24 鯉魚cdc2基因啟動子不同長度片段在胚胎中作用的分佈情形 24 鯉魚JAK1基因啟動子不同區域片段在細胞株及斑馬魚胚胎中的活性比較 28 鯉魚JAK1基因啟動子不同長度片段在胚胎中作用的分佈情形 30 以GFP為檢測基因觀察啟動子的作用 30 第四章 討論 34 參考文獻 43 附錄 52 | |
dc.language.iso | zh-TW | |
dc.title | 魚類基因啟動子在魚類細胞株及斑馬魚胚胎中之活性分析 | zh_TW |
dc.title | Expression of Heterologous Promoter Sequence in Fish Cell Line and Zebrafish Embryos | en |
dc.date.schoolyear | 85-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 70 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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