請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75178
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | 張善喻 | zh_TW |
dc.date.accessioned | 2021-07-01T08:12:06Z | - |
dc.date.available | 2021-07-01T08:12:06Z | - |
dc.date.issued | 2001 | |
dc.identifier.citation | Bishop P. D., Makus D. J., Pearce G. and Ryan C. A. (1981) Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymically released from cell walls. Proc. Nail. Acad. Sci. USA Vol. 78. p.3536-3540. Bishop P. D., Pearce G., Bryant J. E. and Ryan C. A. (1984) Isolation and characterization of the proteinase inhibitor-inducing factor from tomato leaves. J. Biol. Chem. Vol. 259. p.13172-13177. Borut S., Joze P., Pika M., Darja B.-M., Franc G. and Igor K.V.T. (1992) Characterization of aspartic proteinase inhibitors from potato at the gene, cDNA and protein levels. Biol. Chem. Hoppe Seyler Vol. 373. p.477-482. Bradshaw H. D. J., Hollick J. B., Parsons T. J., Clarke H. R. G. and Gordon M. P. (1989) Systemically wound-responsive gene in poplar trees encode proteins similar to sweet potato sporamins and legume kunitz trypsin inhibitors. Plant Mol. Biol. Vol. 14. p.51-59 Broadway R. M. and Duffey S. S. (1986) Plant proteinase inhibitors: mechanism of action and effect on the growth and digestive physiology of larval Heliothis zea and Spodoptera exihua. Journal of Insect Physiology Vol. 32. p.827-833. Burgess E. P. J., Stevens P. 5., Keen G. K., laing W. A. and Christeller J. T. (1991) Effect of protease inhibitors and dietary protein level on the black field cricket Teleogryllus commodus. Entomologia Experimentialis et Appliccata Vol. 61. P. 123-130. Chen M.-S., Johnson B., Wen L., Muthukrishnan S., Kramer K. J., Morgan T. D. and Reeck G. R. (1992) Rice cystatin: bacterial expression, purification, cysteine proteinase inhibitory activity and insect growth suppressing acctivity of a truncated form of the protein. Protein Expression and Purification. Vol. 3. p. 4.1-49. Clough Steven J. and Bent Andrew F. (1998) Floral dip: a simplified method for Agrobacterium-mediated reansformation of Arabidopsis thaliana. The Plant Jouranal Vol.16 (6) p.735-743. Ding L. C., Hu C. Y., Yeh K. W. and Wang P. J. (1998) Development of insect-resistant transgenic cauliflower plants expressing the trypsin inhibitor gene isolated from local sweet potato. Plant Cell Rep. Vol. 17. p.854-860. Edmonds H. S., Gatehouse L. N., Hilder V. A. Aand Gatehouse J. A. (1996) The inhibitory effects of the cysteine protease inhibitor, oryzacystatin, on digestive proteases and larval survival and development of the southern corn rootworm (Diabrotica undecimpunctata howardi). Entomologia Experimentalis e t Applicata. Vol. 78. p.83-94. Elden T. C. (1995) Selected proteinase inhibitor effects on alfalfa weevil (Coleoptera: Curculionidae) Growth and development. Journal Of Economic Entomology. Vol. 88. p.1586-1590. Farmer F. F., Johnson R. R. and Ryan C. A. (1992) Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid. Plant Physiol. Vol. 98. p.995-1002. Flavell R.B. (1994) Review: Inactivation of gene expression in plants as a consequence of specific sequence duplication. Proc. Natl. Acad. Sci. USA Vol.9 1 p.3490-3496. Garcia-Olmedo F., Salcedo G., Sanchez-Monge R., Gomez L., Royo J. and Carbonero P. (1987) Plant proteinaceous inhibitors of proteinase and α-amylase. Oxf Surv. Plant Mol. Cell Biol. Vol. 4. p.345-350. Gatehouse A. M. R., Gatehouse J. A.,Dobie P., Kilminster A. M. and Boulter D. (1979) Biochemical basis of insect resistance in Vigna unguiculata. Journal of the Science of Food and Agriculture. Vol. 30. p.948-958. Gatehouse A. M. R. and Boulter D. (1983) Assessment of the antimetabolic effects of trypsin inhibitors from cowpea (Vigna unguiculata) and other legumes on development of the bruchid bettle Callosobruchus niaculatus. Journal of the Science of Food andAgriculture. Vol. 34. p.345-350. Gatehouse A. M. R., Fenton K. A., Jepson I. and Pavey D. J. (1986) The effects of α-amylase inhibitors on insect storage pests: inhibitor of α-amylase in vitro and effects on development in vivo. Journal of the Science of food and Agriculture Vol.3 7 p.727-734. Gatehouse A. M R., Howe D. S., Flemming J. E., Hilder V. A. and Gatehouse J.A. (1991) Biochemical basis of insect resistance in winged bean (Psophocarpus tetragonolobus) seeds. J. Sci. Food Agric. Vol. 55. p.63-74. Gatehouse A.M.R., Hilder V.A. and Boulter D.(1992) Potential of plant-derived genes in the genetic manipulation of crops for insect resistance. Plant genetic manipulation for crop protection. p.155-187. Gatehouse A. M. R., Davison G. M., Newell C. A., Merryyweather A., Hamilton W. D. O., Burgess E. P. J., Gilbert R. J. C. and Gatehouse J. A. (1997) Transgenic potato plants with enhznced resistance to the tomato month, Lacanobia oleracea: growth room trails. Molecular Breeding Vol. 31. p.49-63. Green T. R. and Ryan C. A. (1971) Wound-induced proteinase inhibitor in plant leaves: a possible defense mechanism against insects. Science Vol.175. p.776-777. Hattori T., Nakagawa T. Maeshima M., Nakamura K. and Asahi T. (1985) Molecular clonging and Nucleotide sequencce of cDNA for sporamin, the major soluble protein of sweet potato tuberous roots. Plant Mol. Biol. Vol. 5. p.313-320. Hattori T., Yoshida N. and Nakamura K. (1989) Structural relationship among the members of a multigene family coding for the sweet potato tub erous root storage protein. Plant Mol. Biol. Vol. 13. p.563-572. Hattori T., Nakagawa S. and Nakamura K. (1990) High-level expression of tuberous root storage protein genes of sweet potato in stem of plantlets grown in vitro on sucrose medium. Plant Mol. Biol. Vol. 14. p.595-604. Hattori T., Fukumoto H., Nakagawa S. and Nakamura K. (1991) Soucrose-induced expression of genes coding for the tuberous root storage protein, sporamin, of sweet potato in leaves and petioles, Plant Cell Physiol. Vol. 32. p.79-86. Hilder V. A., Gatehouse A. M. R., Sheerman S. E., Barker R. F. and Boulter D. (1987) A novel mechanism of insect resistance engineered into tobacco. Nature Vol. 330. p.160-163. Hines M. E., Nielsen S. S., Shade R. E. and pomeroy M. A.(1990) The effect of two proteinase inhibitors, E64 and the BowmanBirk inhibitor, on the developmental time and mortailty of Acanthoscelides obtectus. Entomologia Experimentalis et Appliccaya Vol. 57. p.201-207. Hoffmann Michael P., Zalom Frank G., Wilson Lloyd T., Smilanick Janet M., Malyj Lorraine D., Kiser John, Hilder Vaughan A. and Barnes Wayne M. (1992) Field evaluation of transgenic tobacco containing genes encoding Bacillus thuringiensis δ-endotoxin or cowpea trypsin inhibitor: efficacy against Helicoverpa zea.(Lepidoptera: Noctuidae). J. Econ. Entomol V.85. p. 2516- 2522. Ishiguro S. and Nakamura K. (1992) The nuclear factor SP8BF binds to the 5’-upstream regions of three different genes coding for major proteins of sweet potato tuberous roots. Plant Mol. Biol. Vol. 18. p.97-108. Ishiguro S. and Nakamura K. (1994) Characterization of a cDNA encoding a novel DNA-binding protein, SPF1, that recognizes SP8 sequences in the 5’-upstream regions of genes encoding for sporamin and beta-amylase from sweet potato. Mol. Gen. Genet. Vol. 244. p.563-571. Jakowitch Johannes, Istvan Papp, Eduardo A. M., Johannes van der Windwn, Marjori M. and Antonius J. M. Matzke (1999) Molecular and cytogenetic characterization of a transgene locus that induces silencing and methylation of homologous promoter in trans. The Plant journal V. 17 p.131-141. Johnson R., Narvaez J., An G. and Ryan C. A. (1989) Expression of proteinase inhibitors I and II in transgeni tobacco plants: effects on natural defense against Manduca sexta larvae. Proceedings of the National Academy of Science of the USA Vol. 86. p.9871-9875. Johnston K. A., Gatehouse J. A. and Anstee J. H. (1993) Effects of soybean proteinase inhibitors on the growth and development of larval Helcioverpa armigera. Journal of Insect Physiology. Vol. 39. p.657-664. Jongsma M. A., Bakker P.L., Peters J., Bosch D. and Stiekema W.J. (1995) Adaptation of Spodoptera exigua larvae to plant proteinase inhibitors by induction of proteinase activity insensitive of inhibition. Proceedings of the National Academy of Science of the USA. Vol. 92. p.8041-8045. Jouanin L., Michel B.B., Cecile G., Gil M., and Marc G. (1998) Transgenic plants for insect resistance. Plant Science Vol. 131. p.1-11. Knowles B. H. and Ellar D. J. (1987) Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with differenet insect specificity. Biochen. Biophy Acta Vol.924 p.509-518. Koide Y., Hirano H., Mastsuoka K. and Nakamura K. (1997) The N-terminals propeptide of the precursor of sporamin acts as a vacuole-targeting signal even at the C terminus of the mature part in tobacco cells. Plant Physiol. Vol. 114. p.863-870. Ku Maurice S.B., Agarie Sakae, Nomura Mika, Fukayama Hiroshi, Tsuchida Hiroko, Ono Kazuko, Hirose Sakiko, Miyao Mitsue and Matsuoka Makoto (1999) High-level expression of maize phophoenolpyruvate carboxylase in transgenic rice plants. Nature Biotechnology Vol. 17 p.76-80. Kuroda M., Ishimto M., Suzuki K., Kondo H., Abe K., Kitamura K. and Arai S. (1996) Oryzacystatins exhibit growth-inhibitory and lethal effects on different species of bean insect pest, Ccallosobruchus chinensis (Coleoptera) and Riptortus clavatus (Hemiptera). Bioscience Biotechnology and biochemistry. Vol. 60. p.209-212. Laskowski M, Jr. and kato I. (1980) Protein inhibitors of proteinases. Ann. Rev. Biochem. Vol. 49. p.593-626. Li H. S. and Oba K. (1985) Major soluble proteins of sweet potato roots and changesin in proteins after cutting, infection, or storage. Agric Biol. Chem. Vol. 49. p.737-744. Lin Y. H. and Chu H. H. (1988) Endopeptidases of sprouts and resting roots of sweet potato (Ipomoea batatas (L.) Lam.cv. Tainong 57). J. Chinese Biochem. Soci. Vol. 18. p.18-28. Lin Y. H. (1993) Trypsin inhibitors of sweet potato: review and storage. Recent Adv. Botany Vol. 13. p.179-185. Lyccett G. W.(1990) Genetic engineering of insect control agents and production of insect-resistant plants. Genetic Engineering of Crop Plants. p.49-58. Maarten A. J.,and Bolter C.(1997) The adaptation of insects to plant protease inhibitors. J.insect Physiol. Vol. 43. No.10. p.885-895. Markwick N. P., Reid S. J., Laing W. A. and Christeller J. T. (1995) Effect of dietary protein and protease inhibitors on codling moth (Lepidoptera: Tortricidae). Journal of Economic Entomology Vol. 88. p.33-39 Masehima M., Sasaki T. and Asahi T. (1985) Characterization of major proteins in sweet potato tuberous roots. Phytochemistry Vol. 24. p.1899-1902. Matsuoka K., Matsumoto S., Hattori T., Machida Y. and Nakamura K. (1991) Vacuolar targeting and post-translational precessing of the sweet potato tuberous root storage protein in heterologous plant cells. J. Biol. Chem. Vol.265. p.19750-19757. Matsuoka K. and Kakamura K. (1992) transport of a sweet potato storage protein, sporamin, to the vacuole in yest cells. Plant Cell Physiol. Vol. 33. p.453-462. McGurl B., Orozco-Cardenas M., Pearce G. and Ryan C. A. (1994) Overexpression of the prosystemin gene in transgenic tomato plants generate a systemin signal that constitutively induces proteinase inhibitor sythensis. Proc. Nati. Acad. Sci. USA Vol. 91. p.9799-9802. McManus M. T., White D. W. R. and McGregor P. G. (1994) Accumulation of a chymotrypsin inhibitor in transgenic tobacco can affect the growth of insect pests. Transgenic Research Vol. 3. p.50-58. Nakamura K., Matsuoka K., Mukumoto F. and Watanabe N. (1993) Processing and transport to the vacuole of a preccursor to sweet potato sporamin in transformed tobacco cell line By-2. J.Exp. Botany Vol. 44. p.331-338. Nelson C.E., Walker-simmons M., Makus D., Zuroske G. Grohamand J., and Ryan C.A.(1983) Regulation of synthesis and accumulation of proteinase inhibitors in leaves of wounded tomato plants. In Plant Resistance to Insects, ed. P.A. Hedin, p. 103-122. American Chemical Society, Washington. Odoardi M., Cremona R., Cunico C., Pecetti L., Tava A. and Valdicelli L. (1995) Characterization of trypsin inhibitors in seeds of different Mediccago species. J. Genet. Breeding Vol.48. p.377-382. Ohta S., Hattori T. Morikami A. and Nakamura K. (1991) Hightlevel expression of sweet potato sporamin gene promoter: β-glucuroidase (GUS) fusion gene in the stems of transgenic tobacco plants is conferred by multiple cell type-specific regulatory elements. Mol. Gen. Genet. Vol. 225. p.369-378. Ohto M. Nakamura-Kito K. and Nakamura K. (1992) Induction of expression of genes coding for sporamin and β-amylase by polygalacturonic acid in leaf-petiole cuttings of sweet potato. Plant Physiol. Vol. 99. p.422-427. Pearce G., Johnson S. and Ryan C. A. (1993) Purification and characterization from Tobacco (Nicotiana tabacum) leaves of six small, wound-inducible, proteinase isoinhibitors of the potato inhibitor II family. Plant Physiol. Vol. 102. p.639-644. Ryan C. A.(1981) Protease inhibitor. In the biochemistry of plant Vol. 6. (edit by Marcus A.) p.351-370. New York: Academic Press, Inc. Santos Michael O. , Adang Michael J. , All John N. , Roger Boerma H. and Parrott Wayne A. (1997) Testing transgenes for insect resistance using Arabidopsis. Mol. Breeding Vol.3. p.183-194. Shade R. E. Murdock L. L., Foard D. E. and Pomeroy M.A. (1986) Artifical seed system for bioassay of cowpea weevil (Cole optera: Bruchidae) growth and development. Environmental Entomology Vol. 15. p.1286-1291. Shukle R. H. and Murdocck L. L. (1983) Lipoxygenase, trypsin inhibitor, and lectin from soybeans: effects on larval growth of Manduca sexta (Lepidoptera: Sphingidae). Environmental Entomology Vol. 12. p.787-791 Steffens R., Fox F. R. and Kassell (1978) Effect of trypsin inhibitors on growth and metamorphosis of corn borer larvae Ostrinia nubilalis (Hubner). Journal ofAgricultural Food Chemistry Vol. 26. p.170-174. Tabashnik Bruce E., Finson Naomi, and Johnson Marshall W. (1992) Two protease inhibitors fail to synergize Bacillus thuringirnsis in Diamondback Moth (Lepidoptera: Plutellidae). J. Econ. Entomol V.85. p. 2082-2087. Takeda S., Kowyama Y., Takeucchi Y., Mastuoka K., Nishimura M. and Nakamura K. (1995) Spatial patterns of sucrose-inducible and polygalacturonic acid-inducible expression of genes that encode sporamin and β-amylase in sweet potato. Plant Cell Physiol. Vol. 36. p.321-333. Thomas J. C., Adams D. G., Keppenne V. D., Wasmann C. C., Brown J. K., Kanost M. R. and Bohnert H. J. (1995a) Manduca sexta encoded protease inhibitors expressed in Nicotiana tabacum provide protection against insects. Plant Physiology and Biochemistry Vol. 33. p.611-614. Thomas J. C., Adams D. G., Keppenne V. D., Wasmann C. C., Brown J. K., Kanost M. R. and Bohnert H. J. (1995b) Potease inhibitors of Manduca sexta expressed in transgenic cotton. Plant Cell Reports. Vol. 14. p.758-762. Urwin P. E., Atkinson H. J., Wailer D. A. and McPherson M. J. (1995) engineered oryxacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. Plant Journal Vol. 8. P.121-131. Valdes R. S., Segura N. M., Chagolla L.A., Verver Y. C. C. A., Segura N. M. And Chagolla L.A. (1994) Purification, characterization and complete amino acid sequence of trypsin inhibitor from amaranth (Amaranthus hypochondriacus) seeds. Plant Physiol. Vol. 103. p.1412-1427. Xie Z. W., Luo M. J., Xu W. F. and Chi C. W.(1997) Two reactive site locations and structure-function study of the arrowhead proteinase inhibitors, A and B, using mutagenesis. Biochemistry Vol.36. p.5846-5852. Xu D., Xue Q., McElroy D., Mawal Y., Hilder V. A. and Wu R. (1996) Constitutive expression of a cowpea trypsin inhibitor gene, CpTi, in transgenic rice plants confers resistance to two major rice insect pests. Molecular Breeding Vol. 2. p.167-173. Yang H. L., Luo R. S. and Chi C. W. (1992) Primary structure and disulfide bringe location of Arrowhead double-headed proteinase inhibitors. J. Biochem. Vol.111. p.537-545. Yeh K.W., Chen J. C., Lin M. I., Chen Y. M. and Lin C.Y. (1997a) Functional activity of sporamin from sweet potato (Ipomoea batatas Lam.): a tuber storage protein with trypsin inhibitory activity. Plant Mol. Biol. Vol. 33. p.565-570. Yeh K.W., Lin M.L., Tuan S.J., Chen Y.M., Lin C.Y. and Kao S.S. (1997b) Sweet potato (Ipomea Batatas) trypsin inhibitors expressed intransgenic tobacco plants confer resistance against Spodoptera litura. Plant Cell Rep. Vol.16. p.696-699. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75178 | - |
dc.description.abstract | 面對自然界環境逆境〔stress〕,昆蟲亦能不停地進化出抗性族群。為了能夠減緩昆蟲產生具抗性族群,若利用兩個抗蟲基因轉殖進入作物體內,應該可以有效的輔助植物來抵抗昆蟲,並使昆蟲不易產生抗性族群。 在本實驗中,我們建構三個不同的基因,分別是含SPoramin基因的pCAMBIA1301/sporamin、含Arrowhead Proteinase inhibitor〔API〕基因的pcAMBIA1301/API和結合兩者的 pcAMBIA1301/AL,利用農桿菌〔Agrobacterium tumefaciens〕將基因轉殖進入阿拉伯芥〔Agrobidopsis〕中。經由抗生素hygromycin篩選後,再利用南方點墨法〔Southern blot〕和北方點墨法〔Northern blot〕確認基因有插入及轉錄作用〔transcription〕成mRNA。經由GUS活性分析亦發現具有GUS活性,其胰蛋白抑制脢活性分析〔trypsin inhibitor assay〕亦有活性表現,但卻無法由西方點墨法〔Westhern blot〕和膠體胰蛋白脢活性染〔in situ gel trypsin activity assay〕偵測到蛋白質的存在。 利用阿拉伯芥轉殖株的葉片餵食-齡小菜蛾〔Plutella xylostela〕分析轉殖株的抗蟲能力,發現經過四天的餵食,sporamin轉殖株的存活率53.5%,優於API 轉殖株的59%、AL轉殖株的64.5%,蟲重的分析亦顯示sporamin轉殖株比API和AL轉殖株有效。因此具有雙基因的阿拉伯芥轉殖株並不具有更好的抗蟲效果。 阿拉伯芥轉殖株其蛋白質表現量不佳,可能是載體中的ADH intron所造成。另外一方面,具有sporamin和API的雙基因轉殖株可能有未知的因素造成基因表現的互相幹擾,致使雙基因表現較單一基因差。 | zh_TW |
dc.description.abstract | Facing on the environmental stress, insects evolve the resistant population rapidly. This causes the chemical pesticide and resistant genes invalid shortly. One possible strategy to delay the selection of resistant population is to engineer multiple resistant genes into a single crop cultivar. It is also the way to effectively confer insect resistance on plants. In this study, we use three constructs to perform plant transformation. They are either containing sporamin cDNA gene, i.e. pCAMBIA1301/sporamin, or containing Arrowhead proteinase inhibitor 〔API〕, i.e. pCAMBIA1301/API, and a combinatory construct containing both sporamin and API together, i.e. pCAMBIA13O1/AL. These three constructs were delivered into Arabidopsis respectively by the Agrobacterium tumefaciens-mediated transformation. Many independent transformants of sporamin, API and AL gene were obtained from hygromycin-selection medium. Further identification by Southern blot and Northern blot showed that most transformants were integrated with transgene, and expressed mRNA transcripts effectively. Confirmation by β-glucuronidase 〔GUS〕 activity assays also showed positive results. On the contrary, the presence of TI was not detected from Western blot and in situ gel activity assay. In the bioassay of insect resistance, first-instar insects of Plutella xylostela was fed on leaves of transgenic Arabidopsis. After 4-day feeding, the insect survival rate fed on sporamin is 53.5%, whereas 59% for feeding on API transformants, and 64.5% for feeding on combined API and sporamin transformants. The insect weight also showed that sporamin transformants are more effective than API transformants. Moreover, the insect resistance of transgenic Arabidopsis with combined transgenes was lower than that of plants expressing sporamin or API alone. The low level of transgene expression in Arabidopsis is possibly caused by the presence of ADH intron. However, there might be some unknown factors that interact the gene expression of co-existence of API and sporamin. Therefore, it caused combined gene expressing less than that of the single gene alone. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:12:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2001 | en |
dc.description.tableofcontents | 目錄………………1 中文摘要………………2 英文摘要………………4 前言………………6 材料與方法………………22 結果……………………38 討論……………………59 參考文獻……………………69 附錄…………………………82 | |
dc.language.iso | zh-TW | |
dc.title | 組合型胰蛋白?抑制因數轉殖於阿拉伯芥的抗蟲能力分析 | zh_TW |
dc.title | Testing Combined Trypsin Inhibitor Genes for Insect Resistance using Transgenic Arabidopsis | en |
dc.date.schoolyear | 89-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 84 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
沒有與此文件相關的檔案。
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。