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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kuan-Chung Huang | en |
dc.contributor.author | 黃冠中 | zh_TW |
dc.date.accessioned | 2021-07-01T08:11:23Z | - |
dc.date.available | 2021-07-01T08:11:23Z | - |
dc.date.issued | 1999 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/75016 | - |
dc.description.abstract | 由成熟雄鼠儲精囊分泌液中純化出分子量為6.4kDa的類抑鈣素蛋白?抑制因數(caltrin-like protease inhibitor;簡稱TI),存在於生殖系統中的儲精囊、凝固腺及前列腺。利用雄鼠副睪尾端取出的精子,與生物素(biotin)標示的TI進行免疫組織化學染色,發現TI能專一性結合在精蟲頭部頂體(acrosom)部位。以電腦輔助精蟲活性測定儀(CASA)測定TI與精蟲活動力(motility)的關係,得知精子的活動力與高度活化作用(hyperactivation)會受到TI的抑制而降低,此現象會因加入牛血清白蛋白(BSA)而再度活化。TI與其受體以交又結合試劑(DSS)共價結合後利用西方墨點法分析,測得一15kDa蛋白,為TI結合在精子頂體上的專一性受體。 | zh_TW |
dc.description.abstract | A caltrin-like protease inhibitor (TI) secreted from the accessory sexual glands of male mice was shown to have the ability in the suppression of motility and hyperactivation of mouse spermatozoa. The activity could be counteracted by bovine serum albumin (BSA). We prepared the biotinylated TI as a probe to characterize the TI receptor on sperm surface. The results of both histochemical study and the ligand-receptor crosslinking characterization suggest the presence of a 15kDa TI-binding protein on the sperm head. | en |
dc.description.provenance | Made available in DSpace on 2021-07-01T08:11:23Z (GMT). No. of bitstreams: 0 Previous issue date: 1999 | en |
dc.description.tableofcontents | 中文摘要……………2 英文摘要……………3 縮寫表………………4 論文內容………………6 附圖次…………………8 圖次……………………9 表次……………………10 附錄……………………123 第一章 緒論……………11 1.1 哺乳動物的生殖生理………………13 1.1.1 精子的成熟過程………………13 1.1.2 精子的獲能作用………………14 1.1.3 精子的高度活化作用………………16 1.1.4 精子的頂體反應………………17 1.1.5 卵細胞膜蛋白………………18 1.1.6 精子上相關蛋白研究………………19 1.2 儲精囊分泌蛋白………………21 1.3 Kazal Type胰蛋白?抑制因數………………21 1.4 胰蛋白?抑制因數在生殖生理上的研究………23 1.5 本論文的研究方向………………24 第二章 實驗材料與方法………………35 2.1 胰蛋白?抑制因數的純化………………37 2.1.1 硫酸銨沉澱………………37 2.1.2 分子篩膠體層析法………………38 2.1.3 逆向高效液相層析法………………39 2.1.4 蛋白質定量分析-BCA蛋白質定量法………………40 2.1.5 SDS-聚丙烯醯胺膠體電泳………………42 2.1.6 Coomassie Blue染色………………44 2.1.7 西方墨點法………………44 2.2 胰蛋白?抑制因數之抗體的純化………………47 2.3 胰蛋白?抑制因數的生物素標示作用………………49 2.4 胰蛋白?抑制因數抑制活性的分析………………52 2.5 胰蛋白?抑制因數與精蟲的結合作用………………54 2.6 胰蛋白?抑制因數與精蟲結合之活動力測定…………58 2.7 胰蛋白?抑制因數與精蟲結合之受體研究…………64 第三章 實驗結果………………69 3.1 TI蛋白的純化………………71 3.2 TI抗體的純化………………71 3.3 TI的生物素標示作用………………71 3.4 TI抑制活性的分析………………72 3.5 TI在精子上結合部位………………72 3.6 TI對精蟲活動力的影響………………73 3.7 TI與精子結合受體之鑑定………………73 第四章 討論………………101 第五章 參考文獻………………109 | |
dc.language.iso | zh-TW | |
dc.title | 小白鼠儲精囊分泌胰蛋白?抑制因數與精蟲結合之研究 | zh_TW |
dc.title | A Kazal Type Trypsin Inhibitor-Using a Biotinylated TI as a Probe to Study the Sperm Binding | en |
dc.date.schoolyear | 87-2 | |
dc.description.degree | 碩士 | |
dc.relation.page | 125 | |
dc.rights.note | 未授權 | |
dc.contributor.author-dept | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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