請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74408
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝銘鈞 | |
dc.contributor.author | Shu-Yi Tseng | en |
dc.contributor.author | 曾書怡 | zh_TW |
dc.date.accessioned | 2021-06-17T08:34:11Z | - |
dc.date.available | 2019-08-18 | |
dc.date.copyright | 2019-08-18 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-09 | |
dc.identifier.citation | [1] Kubo Mingliang, 1996, Discovery and Treatment of Gastrointestinal Symptoms, First Edition, Yiqun Publishing, 222-232.
[2] Liao Jiding, 2003, Clinical Oncology, First Edition, Heji Book Publishing, 513-571. [3] Colorectal Cancer - American Cancer Society. [4] Magrini R, Bhonde MR, Hanski M-L, et al. (2002) Cellular effects of CPT-11 on colon carcinoma cells: Dependence on p53 and hMLH1 status. International Journal of Cancer. 101:23-31. [5] Bruce WR, Van Der Gaag HA. (1963) Quantitative assay for the number of murine lymphoma cells capable of proliferation in vivo. Nature. 199:79–80. [6] Visvader JE, Lindeman GJ. (2012) Cancer stem cells: current status and evolving complexities. Cell Stem Cell. 10(6):717–728. [7] Vinogradov S, Wei X. (2012) Cancer stem cells and drug resistane: the potential of nanomedicine, Nanomedicine (LAND). 7(4): 597-615. [8] Paholak HJ, Stevers NO, Chen H, et al. (2016) Elimination of epithelial-like and mesenchymal-like breast cancer stem cells to inhibit metastasis following nanoparticle-mediated photothermal therapy. Biomaterials. 104:145-57 [9] Lee PC, Lin CY, Peng CL, Shieh MJ. (2016) Development of a controlled-release drug delivery system by encapsulating oxaliplatin into SPIO/MWNT nanoparticles for effective colon cancer therapy and magnetic resonance imaging. Biomater Sci. 4(12):1742-1753. [10] Lee SY, Yang CY, Peng CL, Wei MF, Chen KC, Yao CJ, Shieh MJ . (2016) A theranostic micelleplex co-delivering SN-38 and VEGF siRNA for colorectal cancer therapy. Biomaterials. 86:92-105. [11] Wei MF, Chen MW, Chen KC, Lou PJ, Lin SY, Hung SC, Hsiao M, Yao CJ, Shieh MJ. (2014) Autophagy promotes resistance to photodynamic therapy-induced apoptosis selectively in colorectal cancer stem-like cells.Autophagy. 10(7):1179-92. [12] Wang TW, Wu HC, Wang WR, Lin FH, Lou PJ, Shieh MJ, Young TH. (2007) The development of magnetic degradable DP-Bioglass for hyperthermia cancer therapy. J Biomed Mater Res A. 83(3):828-37. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/74408 | - |
dc.description.abstract | 大腸直腸癌目前已是全球人類死亡的重大疾病之一,而國內隨著經濟之進步、飲食習慣之西化,大腸直腸癌之發生率亦隨之增加,約有三分之一的大腸直腸癌患者於診斷時即已發生轉移。而目前大腸直腸癌惡化的元兇乃是因病患體內有一群惡性度極高的腫瘤幹細胞,研究也證實腫瘤幹細胞能造成癌症復發、轉移,以及對藥物產生多重抗藥性的結果。本計畫即是開發一結合診斷與高溫療法、化療之高生物相容性奈米藥物載體以應用於大腸直腸癌與腫 瘤幹細胞之診斷與治療上。本計畫所使用的奈米藥物載體系統是以超順磁磁性奈米粒子 (Superparamagnetic iron oxide nanoparticles)為核心,做為大腸直腸癌診斷與高溫治療之工具,並層層披覆上高生物相容性之 Poly(sodium styrene sulfonate)、化療藥物 Irinotecan (CPT-11)與人類血清白蛋白,並利用 anti-human prominin-1 (PROM1/CD133) monoclonal antibodies 作為此奈米藥物載體對大腸直腸癌標靶治療之標記,以增加直腸腫瘤幹細胞與腫瘤細胞對奈米藥物載體的攝入。故可藉此奈米藥物載體提高化療藥物於血液之半衰期、降低病患之副作用與提高大腸直腸癌熱療與化療之效果。 | zh_TW |
dc.description.abstract | Colorectal cancer is now one of the major diseases in the world. With westernized diet in Taiwan, the incidence of colorectal cancer increases. For cancer stem cell therapy, CD133 (prominin-1) is a theoretical cancer stem cell (CSC) marker for colorectal cancer and is a proposed therapeutic target. Cells with CD133 overexpression have demonstrated enhanced tumor-initiating ability and tumor relapse probability. To resolve the problem of chemotherapy failure, we will develop a magnetite-based nanomedicine using loco-regional hyperthermia combined with chemotherapy. The targeting carrier has a magnetite nanoparticle (superparamagnetic iron oxide nanoparticles, SPIO) core and a layer-by-layer polyelectrolyte molecule shell that carries irinotecan (CPT-11) and anti-human prominin-1 (PROM1/CD133) monoclonal antibody for cancer stem cell-specific targeting. Besides as a contrast agent for MRI, this nanomedicine plays as an important role to relay the externally delivered radiofrequency energy for tumor hyperthermia. Locoregional heat can trigger a drug release from the carrier as it directly damages tumor cells and cancer stem cells. .Finally, the use of this nanomedicine can improve the half-life of chemotherapy drugs in the blood and reduce the side effect, and is significantly more efficacious than hyperthermia or chemotherapy alone for colorectal cancer therapy. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T08:34:11Z (GMT). No. of bitstreams: 1 ntu-108-R06548055-1.pdf: 1363632 bytes, checksum: c2b4a0c44246c971018a017c8685cb0e (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員審定書 I
致謝 II 中文摘要 IV Abstract V Contents VI Chapter 1 Introduction 1 1.1 Research background and its importance 1 1.2 Cancer stem cells 4 1.3 Development of therapeutic system containing superparamagnetic magnetic nanoparticles 5 1.4 Nanomedicine poisoning cancer stem cells 6 1.5Nanomedicine development in hyperthermia 8 Chapter 2 Materials and Methods 10 2.1 Materials 10 2.2 Preparation of SPIOs/PSS/CPT11/HAS-AntiCD133 10 2.3 Cell culture 11 2.4 Flow Cytometry 11 2.5 In vitro radio frequency properties 12 2.6 In vitro cytotoxicity of magnetic hyperthermia and chemotherapy 12 2.7 Prussian blue staining 14 2.8 Animal and tumor model 14 2.9 T2-MRI image 15 2.10 In vivo antitumor efficacy 16 2.11 Statistical analysis 17 Chapter 3 Result and Discussion 18 3.1 Preparation and Characteristics of SPIO@APTES and SPIOs/PSS/CPT11/HAS-AntiCD133 NPs 18 3.2 T2-MRI image of SPIO@APTES, SPIOs/PSS/CPT11/HAS NPs and SPIOs/PSS/CPT11/HAS-AntiCD133 NPs 20 3.3 Anti-CD133 expression on the CRC cells 20 3.4 In vitro cytotoxicity of prepared nanoparticles combined with RF magnetic field irradiation 21 3.5 Cellular uptake 22 3.6 In vivo T2-weight MRI image 23 3.7 In vivo antitumor effect 24 Chapter 4 Conclusion 25 Reference 26 Scheme 28 Tables 30 Figures 32 | |
dc.language.iso | en | |
dc.title | 結合癌症診斷與磁熱化療之氧化鐵奈米藥物載體開發 | zh_TW |
dc.title | Magnetite-based nanomedicine for cancer diagnosis and therapy using loco-regional hyperthermia combined with chemotherapy | en |
dc.type | Thesis | |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊台鴻,林文澧,張富雄 | |
dc.subject.keyword | 大腸直腸癌,腫瘤幹細胞,高溫療法,化學治療,超順磁磁性奈米粒子, | zh_TW |
dc.subject.keyword | colorectal cancer,cancer stem cell,hyperthermia,chemotherapy,superparamagnetic iron oxide nanoparticles,irinotecan, | en |
dc.relation.page | 42 | |
dc.identifier.doi | 10.6342/NTU201902952 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2019-08-12 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-108-1.pdf 目前未授權公開取用 | 1.33 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。