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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 黃青真 | |
dc.contributor.author | Feng-Lung Tsai | en |
dc.contributor.author | 蔡豐隆 | zh_TW |
dc.date.accessioned | 2021-06-16T17:30:36Z | - |
dc.date.available | 2017-08-27 | |
dc.date.copyright | 2012-08-27 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-15 | |
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Momordica charantia (bitter melon) reduces plasma apolipoprotein B-100 and increases hepatic insulin receptor substrate and phosphoinositide-3 kinase interactions. Br J Nutr. 2008 Mar. 5;100(04). 16. Xu X, Harris KS, Wang HJ, Murphy PA, Hendrich S. Bioavailability of soybean isoflavones depends upon gut microflora in women. The Journal of nutrition. 1995 Sep. 1;125(9):2307–15. 17. Zhang JM, Matsuura Y, Sueda T, Orihashi K. Beneficial effects of ginsenosides of stems and leaves on cardiac and coronary vascular functions after 12-hour rat heart preservation. Transplant. Proc. 1999 Aug.;31(5):2175–8. 18. Kawakami Y, Tsurugasaki W, Nakamura S, Osada K. Comparison of regulative functions between dietary soy isoflavones aglycone and glucoside on lipid metabolism in rats fed cholesterol. J. Nutr. Biochem. 2005 Apr. 1;16(4):205–12. 19. Tawab MA. DEGRADATION OF GINSENOSIDES IN HUMANS AFTER ORAL ADMINISTRATION. Drug Metabolism and Disposition. 2003 Aug. 1;31(8):1065–71. 20. 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Samanya M, Yamauchi K-E. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 2002 Sep.;133(1):95–104. 31. Hosoi T, Ametani A, Kiuchi K, Kaminogawa S. Changes in fecal microflora induced by intubation of mice with Bacillus subtilis (natto) spores are dependent upon dietary components. Can J Microbiol. 1999 Jan.;45(1):59–66. 32. Katsuyama H, Ideguchi S, Fukunaga M, Saijoh K, Sunami S. Usual dietary intake of fermented soybeans (Natto) is associated with bone mineral density in premenopausal women. J. Nutr. Sci. Vitaminol. 2002 Jun.;48(3):207–15. 33. SUMI H. Accumulation of Vitamin K(Menaquinone-7) in Plasma after Ingestion of Natto and Natto Bacilli(B. subtilis natto). FSTR. 1999;5(1):48–50. 34. Heinonen S, Adlercreutz H. Identification of isoflavone metabolites dihydrodaidzein, dihydrogenistein, 6'-OH-O-dma, and cis-4-OH-equol in human urine by gas chromatography-mass …. Analytical biochemistry. 1999. 35. Ibe S, Kumada K, Yoshiba M. Production of natto which contains a high level of isoflavone aglycons. … -JAPANESE SOCIETY OF …. 2001. 36. Ramalhete C, Mansoor TA, Mulhovo S, Molnár J, Ferreira M-JU. Cucurbitane-Type Triterpenoids from the African Plant Momordica balsamina. Journal of Natural Products. 2009 Nov. 30;72(11):2009–13. 37. Sumi H. Antibacterial Activity of Bacillus natto-Growth inhibition against Escherichia coli-O157. Bioindustry; 1997. 38. JI N, KONG F, ZU G, JI Y. THE PRESENT SITUATION AND DEVELOPMENTAL TENDENCY OF ANTIMICROBIAL FUNC-TIONS OF BACILLUS NATTO [J]. Food Research and …. 2006. 39. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J. Nutr. 1993. p. 1939–51. 40. Handa G, Singh J, Sharma M, Kaul A. Hypoglycemic principle of Momordica charantia seeds. Indian J. Nat. Prod; 1990. 41. Meir P, Yaniv Z. An in vitro Study on the Effect of Momordica charantia on Glucose Uptake and Glucose Metabolism in Rats. Planta Med. 1985 Feb.;51(1):12–6. 42. Welininda J, Avvidoon G, Gylfe E, Hellings B, Karlsson E. 1982. The insulin-releasing activity of the tropical plant Momordica charantia. Acta Biol Med 41:1229-40 43. 呂佩諭 (2008). 篩選最具改善糖尿病前期患者血糖、血脂異常之苦瓜品系。Unpublished 44. 許珊菁 (2006). 鼠模式中高脂飲食、肥胖與脂質調控基因之表現.國立台灣大學微生物與生化學研究所博士論文. 45. 黃婷妮 (2010). 山苦瓜萃物暨其區分物之腸泌素效應. 國立台灣大學微生物與生化學研究所博士論文. 國立台灣大學微生物與生化學研究所碩士論文. 46. 周怡君 (2010). 以脂肪與肌肉細胞模式評估山苦瓜水萃物暨其區分物對細胞汲取葡萄糖之影響與其機制探討. 國立台灣大學微生物與生化學研究所碩士論文. 47. 楊惟蒂 (2010). 山苦瓜水萃物暨其區分物對肝細胞汲取葡萄醣及胰島beta細胞分泌胰島素之影響. 國立台灣大學微生物與生化學研究所碩士論文. 48. 黃翠萍 (2001). 土產蔬菜之抗菌活性調查及苦瓜抗菌活性成分之研究. 國立台灣大學微生物與生化學研究所碩士論文. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64114 | - |
dc.description.abstract | 第二型糖尿病為一種代謝性疾病,臨床上以禁食血糖超過126 mg/dl為診斷依據,病發前會經歷一段過渡期稱之為糖尿病前期。此時期若以適當運動、生活型態改變以及適當之飲食攝取,能有效降低其未來發生第二型糖尿病之發生機率。
許多研究顯示苦瓜具有降血糖作用,其中含有多種降血糖效果之活性成分,不同活性成分會透過不同機制而達到降血糖效果。早期的研究結果顯示苦瓜調節血糖之活性成份乃是charantin、vicine與poplypeptide-p等,最近有許多文獻著重於苦瓜中葫蘆烷型三萜類化合物 (cucurbitane-type triterpenoid) 對於降血糖效果之探討,其中主要是短鏈及不帶醣基之三萜類化合物。本實驗室過去研究顯示,經市售之β-glucosidase水解山苦瓜水萃物後之乙酸乙酯萃物更有效促進單一劑量投予動物實驗及以細胞平台之增加肝細胞的葡萄糖汲取、β-cell 的胰島素分泌及促進脂肪與肌肉細胞汲取葡萄糖的活性。基於苦瓜中三萜類成份多以帶醣基之型式存在,故本研究乃以增加短鏈及不帶醣基之三萜類降血糖活性成分為目標,希望能建立更有效之去醣基方法。本系李昆達教授實驗室曾由一般市售納豆產品中分離出具有高β-glucosidase活性之納豆菌Bacillus subtilis natto NTU-18。故本研究乃將用此納豆菌株為酵素來源,觀察其加入至山苦瓜萃取物中,是否能有效的進行去醣基作用以及經水解後山苦瓜萃取物之降血糖效果探討。 結果顯示,滅菌山苦瓜果汁具有抑制納豆菌生長之成份,因此不能將納豆菌直接處理山苦瓜果汁或水萃物中。因此,為排除山苦瓜抗菌之作用,先將納豆菌培養於5%黑豆漿13-15小時,待酵素活性開始增加,在將納豆菌離心出來放入滅菌山苦瓜果汁或者不經加熱滅菌山苦瓜水萃物。經此等處理之山苦瓜萃物在薄層層析分析結果顯示,5%黑豆漿培養之納豆菌可改變山苦瓜萃物乙酸乙酯萃取物之組成成份。LC-ToF-MS分析顯示,經納豆菌處理之滅菌山苦瓜果汁乙酸乙酯萃物可有效增加短鏈及不帶醣基之三萜類化合物含量,且部分化合物為先前文獻指出據有降血糖活性效果。最後,將經過5%黑豆漿培養納豆菌處理後之山苦瓜水萃物及區分物以單劑量投予,經30%高油飲食誘發之肥胖小鼠,觀察其對口服葡萄糖耐受性之影響。實驗結果顯示,5%黑豆漿培養納豆菌處理後之山苦瓜水萃物在劑量4200 mg/kg BW顯著改善口服葡萄糖耐受性,其中乙酸乙酯萃取物亦具有顯著效果,而未經水解者則否。且未經5%黑豆漿培養之納豆菌處理不加熱山苦瓜水萃物其乙酸乙酯萃取物亦不具有降血糖效果。 綜合以上結論,本研究為首次建立納豆菌處理山苦瓜萃取物中。以經過5%黑豆漿培養之納豆菌對滅菌山苦瓜果汁進行水解可增加短鏈及不帶醣基之三萜類化合物。此外,以相同之納豆菌水解模式處理之不加熱山苦瓜水萃物,可改善口服葡萄糖耐受性,且其乙酸乙酯萃物亦具有效果。本研究證實以大量表現β-glucosidase 納豆菌株對山苦瓜進行去醣基作用,具有可行性。 | zh_TW |
dc.description.abstract | Type 2 diabetes mellitus is a metabolic disease characterized by hyperglycemia due primarily to insulin resistance. A period of prediabetic condition usually precede that can be intervened with diet and life style modification.
Many studies have reported that Momordica charantia (Bitter melon, BM) has a hypoglycemic effect. Early results suggested the hypoglycemic ingredients of bitter melon might be charantin, vicine and poplypeptide-p etc. Recent reports focused on the cucurbitane type triterpenoid isolation from bitter melon, especially those without or with only one glycoside conjugates. Our previous studies showed that water extract of BM treated with commercial β-glucosidase showed better glucose regulating activities in various cell models and single dose administration mouse studies. In addition, Professor Lee’s laboratory isolated a strain of Bacilius natto NTU-18 that can express high level of β-glucosidase. Based on the fact that most triterpenoids exist as glycosides form in BM, this study aims to establish a bioprocess procedure using high β-glucosidase acitivity Bacilius natto NTU-18 to hydrolyze BM extracts. In the attempt to cultivate Bacilius natto NTU-18 in autoclaved BM juice, a growth inhibition was observed. The strategy was then modified to cultivate Bacilius natto NTU-18 first in 5% black soymilk for 13-15 hours, while the β-glucosidase enzyme activity was induced. The activated Bacilius natto NTU-18 was then transferred to autoclaved BM juice or water extract (WE). Different TLC pattern of the ethyl acetate (EA) extracts of these processed BG juice suggested this process produced low polarity compounds. LC-ToF-MS analysis further indicated increases in the short chain and aglycone triterpenoids. Finally, C57BL/6 male mice fed a 30% high fat diet to induce hyperglycemia were administered a single dose of the bioprocessed BG WE to test its effects on glucose tolerance. Both 4200 and 3000 mg/kg BW doses of BG WE treated with activated Bacilius natto NTU-18 .significantly improved oral glucose tolerance in these mice. The EA extract of the Bioprocessed BG WE showed similar effect. In conclusion, this is the first attempt to develop a bioprocess to hydrolyze triterpenoid glycosides of BM. The bioprocess employing Bacilius natto NTU-18 precultured in 5% black soymilk to treat bitter melon juice can increase the short-chain and aglycone triterpenoid. The bioprocessed BG WE products was shown to improve oral glucose tolerance in a single dose administration test in mice. Results of this study support the feasibility of the bioprocess using precultured Bacilius natto NTU-18. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:30:36Z (GMT). No. of bitstreams: 1 ntu-101-R99b22033-1.pdf: 2586420 bytes, checksum: fe31c264b0a08c0986f43dc35135c66d (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 中文摘要 i
Abstract iii 縮寫對照表 v 總目錄 vi 圖目錄 ix 表目錄 xi 第一章 緒論 1 第一節 前言 1 第二節 文獻回顧 2 一、 糖尿病 2 二、 苦瓜 3 三、 酵素水解 7 四、 β-glucosidase 7 五、 納豆菌Bacillus subtilis natto 11 第三節 研究目的 12 第四節 實驗架構 12 第一章:將納豆菌加入山苦瓜萃取物中進行去醣基之模式建立。 12 第二章:單一劑量投予經納豆菌處理之山苦瓜水萃物及區分物之降血糖效果初步探討。 12 第二章 納豆菌加入山苦瓜萃取物中進行去醣基之模式建立 13 第一節 前言 13 第二節 材料與方法 14 一、 實驗架構 14 二、 試劑 14 三、 儀器 15 四、 山苦瓜萃取物之製備 15 五、 納豆菌培養 15 六、 經5%黑豆漿培養之納豆菌處理山苦瓜萃物進行去醣基作用。 16 七、 薄層層析 17 八、 LC-ToF-MS分析 17 第三節 實驗結果 19 一、 納豆菌培養於加熱滅菌山苦瓜果汁之效果探討 19 二、 5%黑豆漿活化之納豆菌處理滅菌山苦瓜果汁進行水解作用 19 三、 5% BS培養之納豆菌處理不加熱山苦瓜水萃物之水解作用效果探討 20 第四節 討論 32 一、 滅菌山苦瓜果汁對納豆菌生長之影響 32 二、 經5%黑豆漿培養之納豆菌處理山苦瓜果汁進行去醣基作用之效果探討 32 三、 納豆菌處理不加熱滅菌山苦瓜水萃物之生長與去醣基作用之效果探討 35 第五節 結論 36 第三章 單一劑量投予經納豆菌處理之山苦瓜水萃物及區分物調節血糖效果初步探討 38 第一節 前言 38 第二節 材料與方法 39 一、 實驗架構 39 二、 管餵樣品製備 39 三、 動物飼養 40 四、 飼料 41 五、 經納豆菌作用之山苦瓜萃取物投予對於口服葡萄糖耐受性之測試 41 六、 血糖分析 42 七、 統計分析 42 第三節 實驗結果 43 一、 經5%黑豆漿培養之納豆菌水解之山苦瓜水萃物之劑量測試 43 二、 經5%黑豆漿培養之納豆菌水解山苦瓜水萃取物之各區分物調節血糖之活性探討 43 三、 有無經5%黑豆漿培養之納豆菌處理山苦瓜水萃取物之乙酸乙酯萃取物調節血糖活性之探討 44 第四節 討論 48 第四章 綜合討論 50 第一節 綜合討論 50 第二節 總結論 51 第五章 附錄 52 第六章 參考資料 83 | |
dc.language.iso | zh-TW | |
dc.title | 山苦瓜萃取物經納豆菌NTU-18去醣基化作用之效果探討 | zh_TW |
dc.title | Study of Deglucosylation of Momordica Charantia L. extracts by Bacillus subtilis natto NTU-18 | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林璧鳳,李昆達,蕭明熙,楊健志 | |
dc.subject.keyword | 山苦瓜,降血糖,葫蘆烷型三萜,類化合物,β-glucosidase,納豆菌NTU-18, | zh_TW |
dc.subject.keyword | Momordica charantia,hypoglycemic,Bacilius natto NTU-18,cucurbitane type triterpenoid,β-glucosidase, | en |
dc.relation.page | 86 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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