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標題: | 壬基酚與辛基酚對阿拉伯芥生長與蛋白質體影響之研究 Effect of Nonylphenol and Octylphenol on Growth and Proteome of Arabidopsis thaliana |
作者: | Bing-Sheng Chen 陳炳勝 |
指導教授: | 顏瑞泓 |
關鍵字: | 壬基酚,辛基酚,阿拉伯芥,植物逆境,蛋白質二維電泳, nonylphenol,octylphenol,Arabidopsis thaliana,plant stress,2-D gel electrophoresis, |
出版年 : | 2013 |
學位: | 博士 |
摘要: | 本研究主要內容為研究壬基酚 (Nonylphenol, NP) 與辛基酚 (Octylphenol, OP) 對模式植物阿拉伯芥的影響。結果顯示在10天的處理期中,阿拉伯芥的生長受到 NP 與 OP 的抑制,反映在葉片的外觀、鮮重、乾重與根長上。又 NP 與 OP造成了葉綠素含量與葉片相對含水量的下降,同時脯胺酸濃度 (滲透逆境指標)亦增加。本研究結果顯示 NP 與 OP 會造成阿拉伯芥葉部H2O2與MDA的累積,顯示此兩種化合物皆會對阿拉伯芥造成氧化逆境。在NP所引起的氧化逆境中,阿拉伯芥會增加抗氧化酵素ascorbate peroxidase, catalase, CuZnSOD 與 MnSOD 的活性來抵抗氧化逆境,而當 NP 濃度高達50 mg L-1時,SOD 活性會大幅下降,而造成 MDA 的顯著上升,OP也有類似的情形,此結果顯示阿拉伯芥對壬基酚與辛基酚有相同的反應模式。本研究利用蛋白質二維電泳技術 (2-D gel electrophoresis) 與質譜分析法(mass spectrometry) 分析在NP與OP處理下,阿拉伯芥地上部蛋白質體的變化,結果發現NP 會影響 sedoheptulose 1,7-bisphosphatase, fructose-1,6-biphosphate aldolase, Rubisco 與 oxygen-evolving enhancer protein 的表現量,上述四種蛋白皆為光合作用中重要的酵素,可發現 NP 抑制阿拉伯芥生長的原因係對光合成系統造成傷害。OP處理則會影響Anthranilate synthase component I-2, chloroplastic, Methyl-CpG-binding domain-containing protein 2 與pentatricopeptide repeat-containing protein,上述三種蛋白質主要功能為參與葉綠素的合成與植物的生長,則可發現 OP 抑制阿拉伯芥生長的原因為影響光合成系統與擾亂植物生長相關蛋白質。
本研究結果指出對動物而言具有內分泌干擾作用之 NP 與 OP 兩種環境荷爾蒙化合物,對植物亦會造成相當程度的損傷,因此研究其在環境中之殘留與流佈問題時除需了解其對動物的內分泌干擾作用外,其對植物所造成的危害值得持續的重視與審慎的評估。 We studied the effects of nonylphenol (NP) and octylphenol (Giannopolitis and Ries) on physical features and proteome of Arabidopsis (Arabidopsis thaliana) during growth. Biomass and root length were decreased after 10 days of NP treatment. Levels of chlorophyll decreased but proline increased in leaves. Malondialdehyde (MDA), is oxidative stress indicators. The results indicated that NP and OP caused oxidative stress, and the activities of ascorbate peroxidase, catalase, CuZnSOD and MnSOD were induced in leaves under NP stress. The observed decrease in SOD activity with 50 mg L-1 NP treatment could diminish the ability of the Arabidopsis scavenge O2- radicals, thus favoring an accumulation of ROS, which could cause MDA accumulation. OP also caused similar results. Arabidopsis has the same reaction mode against NP and OP. The proteome of leaf tissue was analyzed by 2-D gel electrophoresis and mass spectrometry. NP might adversely affect the activities of sedoheptulose 1,7-bisphosphatase, fructose-1,6-biphosphate aldolase, Rubisco and oxygen-evolving enhancer protein. The above enzymes are important enzymes in photosynthesis. OP adversely affects the activities of Anthranilate synthase component I-2, chloroplastic, Methyl-CpG-binding domain-containing protein 2 and pentatricopeptide repeat-containing protein. The above enzymes are important enzymes in chlorophyll synthesis and plant growth. The results can solve that NP and OP inhibit growth of Arabidopsis. The results of this study indicated that NP and OP are not only endocrine disruption of animals, but also result in a considerable degree of plants damage. The residual in environment deserves continued attention and concern. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63045 |
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