請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55480
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
dc.contributor.author | Yi-Tse Liu | en |
dc.contributor.author | 劉以則 | zh_TW |
dc.date.accessioned | 2021-06-16T04:04:51Z | - |
dc.date.available | 2014-10-03 | |
dc.date.copyright | 2014-10-03 | |
dc.date.issued | 2014 | |
dc.date.submitted | 2014-09-25 | |
dc.identifier.citation | Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR., 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 301(5641):1849.
Amirsadeghi S, Robson CA, Vanlerberghe GC., 2007. The role of the mitochondrion in plant responses to biotic stress. Physiol. Plant. 129, 253-266. Balandrin MF, Klocke JA, Wurtele ES, Bollinger WH., 1985. Natural plant chemicals: sources of industrial and medicinal materials. Science. 228(4704):1154-60. Banerjee S, Singh S, Ur Rahman L., 2012. Biotransformation studies using hairy root cultures - A review. Biotechnol Adv. 30(3):461-8. Bonhomme V, Laurain-Mattar D, Fliniaux MA., 2000. Effects of the rolC gene on hairy root: induction development and tropane alkaloid production by Atropa belladonna. J Nat Prod. 63(9):1249-52. Bulgakov VP, Aminin DL, Shkryl YN, Gorpenchenko TY, Veremeichik GN, Dmitrenok PS, Zhuravlev YN., 2008. Suppression of reactive oxygen species and enhanced stress tolerance in Rubia cordifolia cells expressing the rolC oncogene. Mol Plant Microbe Interact. 21(12):1561-70. Bulgakov VP, Tchernoded GK, Mischenko NP, Shkryl YN, Glazunov VP, Fedoreyev SA, Zhuravlev YN., 2003. Effects of Ca (2+) channel blockers and protein kinase/phosphatase inhibitors on growth and anthraquinone production in Rubia cordifolia callus cultures transformed by the rolB and rolC genes. Planta. 217(3):349-55. Bulgakov VP, Tchernoded GK, Mischenko NP, Khodakovskaya MV, Glazunov VP, Radchenko SV, Zvereva EV, Fedoreyev SA, Zhuravlev YN., 2002. Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes. J Biotechnol. 97(3):213-21. Cardarelli M, Mariotti D, Pomponi M, Spanò L, Capone I, Costantino P., 1987. Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype. Mol Gen Genet. 209(3):475-80. Casanova E, Valdés AE, Zuker A, Fernández B, Vainstein A, Trillas MI, Moysset L., 2004. rolC-transgenic carnation plants: adventitious organogenesis and levels of endogenous auxin and cytokinins. Plant Sci. 167, 551-60. Casanova E, Zuker A, Trillas MI, Moysset L, Vainstein A., 2003. The rolC gene in carnation exhibits cytokinin- and auxin-like activities. Sci Hortic. 97, 321-31. Castle LA, Smith KD, Morris RO., 1992. Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule. J Bacteriol. 174(5):1478-86. Chandra S., 2012. Natural plant genetic engineer Agrobacterium rhizogenes: role of T-DNA in plant secondary metabolism. Biotechnol Lett. 34(3):407-15. Conn HJ., 1942. Validity of the Genus Alcaligenes. J Bacteriol. 44(3):353-60. De Clercq I, Vermeirssen V, Van Aken O, Vandepoele K, Murcha MW, Law SR, Inzé A, Ng S, Ivanova A, Rombaut D, van de Cotte B, Jaspers P, Van de Peer Y, Kangasjärvi J, Whelan J, Van Breusegem F., 2013. The membrane-bound NAC transcription factor ANAC013 functions in mitochondrial retrograde regulation of the oxidative stress response in Arabidopsis. Plant Cell. 25(9):3472-90. Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS., 2006. Gateway-compatible vectors for plant functional genomics and proteomics. Plant J. 45(4):616-29. Estruch JJ, Chriqui D, Grossmann K, Schell J, Spena A., 1991a. The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. EMBO J. 10(10):2889-95. Estruch JJ, Paretssoler A, Schmulling T, Spena A., 1991b. Cytosolic localization in transgenic plants of the rolC peptide from Agrobacterium rhizogenes. Plant Mol Biol. 17(3):547-50. Faiss M, Strnad M, Redig P, Dolezal K, Hanus J, Onckelen H, Schmulling T., 1996. Chemically induced expression of the rolC-encoded β-glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosides in planta. Plant J. 10, 33-46. Flores HE, Vivanco JM, Loyola-Vargas VM., 1999. 'Radicle' biochemistry: the biology of root-specific metabolism. Trends Plant Sci. 4(6):220-226. Giraud E, Ho LH, Clifton R, Carroll A, Estavillo G, Tan YF, Howell KA, Ivanova A, Pogson BJ, Millar AH, Whelan J., 2008. The absence of ALTERNATIVE OXIDASE1a in Arabidopsis results in acute sensitivity to combined light and drought stress. Plant Physiol. 147(2):595-610. Giri A, Dhingra V, Giri CC, Singh A, Ward OP, Narasu ML., 2001. Biotransformations using plant cells, organ cultures and enzyme systems: current trends and future prospects. Biotechnol Adv. 19(3):175-99. Gorpenchenko TY, Kiselev KV, Bulgakov VP, Tchernoded GK, Bragina EA, Khodakovskaya MV, Koren OG, Batygina TB, Zhuravlev YN., 2006. The Agrobacterium rhizogenes rolC-gene-induced somatic embryogenesis and shoot organogenesis in Panax ginseng transformed calluses. Planta. 223(3):457-67. Guillon S, Tremouillaux-Guiller J, Pati PK, Rideau M, Gantet P., 2006. Hairy root research: recent scenario and exciting prospects. Curr Opin Plant Biol. 9(3):341-6. Hansen G, Vaubert D, Clerot D, Tempe J, Brevet J., 1994. A new open reading frame, encoding a putative regulatory protein, in Agrobacterium rhizogenes T-DNA. C R Acad Sci III. 317(1):49-53. Hirayama T, Matsuura T, Ushiyama S, Narusaka M, Kurihara Y, Yasuda M, Ohtani M, Seki M, Demura T, Nakashita H, Narusaka Y, Hayashi S., 2013. A poly(A)-specific ribonuclease directly regulates the poly(A) status of mitochondrial mRNA in Arabidopsis. Nat Commun. 4:2247. Hong SB, Hwang I, Dessaux Y, Guyon P, Kim KS, Farrand SK., 1997. A T-DNA gene required for agropine biosynthesis by transformed plants is functionally and evolutionarily related to a Ti plasmid gene required for catabolism of agropine by Agrobacterium strains. J Bacteriol. 179(15):4831-40. Hu R, Qi G, Kong Y, Kong D, Gao Q, Zhou G., 2010. Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa. BMC Plant Biol. 10:145. Hu ZB, Du M., 2006. Hairy root and its application in plant genetic engineering. J Integr Plant Biol. 48, 121-7. Hughes EH, Hong SB, Gibson SI, Shanks JV, San KY., 2004. Metabolic engineering of the indole pathway in Catharanthus roseus hairy roots and increased accumulation of tryptamine and serpentine. Metab Eng. 6(4):268-76. Jouanin L, Guerche P, Pamboukdjian N, Tourneur C, Delbart FC, Tourneur J., 1987. Structure of T-DNA in plants regenerated from roots transformed by Agrobacterium rhizogenes strain A4. Mol Gen Genet. 206, 387-92. Kaldorf M, Fladung M, Muhs HJ, Buscot F., 2002. Mycorrhizal colonization of transgenic aspen in a field trial. Planta. 214(4):653-60. Keresztessy Z, Hughes J, Kiss L, Hughes MA., 1996. Co-purification from Escherichia coli of a plant beta-glucosidase-glutathione S-transferase fusion protein and the bacterial chaperonin GroEL. Biochem J. 314 (1):41-7. Kiselev KV, Dubrovina AS, Veselova MV, Bulgakov VP, Fedoreyev SA, Zhuravlev YN, 2007. The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells. J Biotechnol. 128(3):681-92. Kiselev KV, Gorpenchenko TIu, Chernoded GK, Dubrovina AS, Grishchenko OV, Bulgakov VP, Zhuravlev IuN., 2008. Calcium-dependent mechanism of somatic embryogenesis in oncogene rolC expressing cell cultures of Panax ginseng. Mol Biol. 42(2):275-85. Kiselev KV, Kusaykin MI, Dubrovina AS, Bezverbny DA, Zvyagintseva TN, Bulgakov VP., 2006. The rolC gene induces expression of a pathogenesis-related beta-1,3-glucanase in transformed ginseng cells. Phytochemistry. 67(20):2225-31. Lee LY, Gelvin SB., 2008. T-DNA binary vectors and systems. Plant Physiol. 146(2):325-32. Licausi F, van Dongen JT, Giuntoli B, Novi G, Santaniello A, Geigenberger P, Perata P., 2010. HRE1 and HRE2, two hypoxia-inducible ethylene response factors, affect anaerobic responses in Arabidopsis thaliana. Plant J. 62(2):302-15. Lima JE, Benedito VA, Figueira A, Peres LE., 2009. Callus, shoot and hairy root formation in vitro as affected by the sensitivity to auxin and ethylene in tomato mutants. Plant Cell Rep. 28(8):1169-77. Maurel C, Barbierbrygoo H, Spena A, Tempe J, Guern J., 1991. Single rol Genes from the Agrobacterium rhizogenes T(L)-DNA Alter Some of the Cellular Responses to Auxin in Nicotiana tabacum. Plant Physiol. 97(1):212-6. Maxwell DP, Wang Y, Mcintosh L., 1999. The alternative oxidase lowers mitochondrial reactive oxygen production in plant cells. Proc Natl Acad Sci U S A. 96(14):8271-6. Moore AL, Albury MS, Crichton PG, Affourtit C., 2002. Function of the alternative oxidase: is it still a scavenger? Trends Plant Sci. 7(11):478-81. Murashige T, Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15, 473-97. Nakano T, Suzuki K, Fujimura T, Shinshi H., 2006. Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiol. 140(2):411-32. Nakashima K, Takasaki H, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K., 2012. NAC transcription factors in plant abiotic stress responses. Biochim Biophys Acta. 1819(2):97-103. Nilsson O, Crozier A, Schmulling T, Sandberg G, Olsson O., 1993a. Indole-3-acetic acid homeostasis in transgenic tobacco plants expressing the Agrobacterium rhizogenes rolB gene. Plant J. 3, 681-9. Nilsson O, Moritz T, Imbault N, Sandberg G, Olsson O, 1993b. Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA. Plant Physiol. 102(2):363-371. Nuruzzaman M, Sharoni AM, Kikuchi S., 2013. Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants. Front Microbiol. 4:248. O'malley RC, Alonso JM, Kim CJ, Leisse TJ, Ecker JR., 2007. An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome. Nat Protoc. 2(11):2910-7. Ogawa T, Pan L, Kawai-Yamada M, Yu LH, Yamamura S, Koyama T, Kitajima S, Ohme-Takagi M, Sato F, Uchimiya H., 2005. Functional analysis of Arabidopsis ethylene-responsive element binding protein conferring resistance to Bax and abiotic stress-induced plant cell death. Plant Physiol. 138(3):1436-45. Oller ALW, Agostini E, Talano MA, Capozucca C, Milrad SR, Tigier HA, Medina MI., 2005. Overexpression of a basic peroxidase in transgenic tomato (Lycopersicon esculentum Mill. cv. Pera) hairy roots increases phytoremediation of phenol. Plant Sci. 169, 1102-11. Ono NN, Tian L., 2011. The multiplicity of hairy root cultures: prolific possibilities. Plant Sci. 180(3):439-46. Pandolfini T, Molesini B, Avesani L, Spena A, Polverari A., 2003. Expression of self-complementary hairpin RNA under the control of the rolC promoter confers systemic disease resistance to plum pox virus without preventing local infection. BMC Biotechnol. 3:7. Park HY, Seok HY, Woo DH, Lee SY, Tarte VN, Lee EH, Lee CH, Moon YH., 2011. AtERF71/HRE2 transcription factor mediates osmotic stress response as well as hypoxia response in Arabidopsis. Biochem Biophys Res Commun. 414(1):135-41. Park SU, Kim YK, Jang HG, Kim JN, Ryu HW., 2008. Auxin treatment improves indigo biosynthesis in hairy root cultures of Polygonum tinctorium. Chem. Nat. Compd. 44, 272-3. Puranik S, Sahu PP, Srivastava PS, Prasad M., 2012. NAC proteins: regulation and role in stress tolerance. Trends Plant Sci. 17(6):369-81. Schmulling T, Fladung M, Grossmann K, Schell J., 1993. Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of Agrobacterium rhizogenes T-DNA. Plant J. 3, 371-82. Schmulling T, Schell J, Spena A, 1988. Single genes from Agrobacterium rhizogenes influence plant development. EMBO J. 7(9):2621-9. Shkryl YN, Veremeichik GN, Bulgakov VP, Tchernoded GK, Mischenko NP, Fedoreyev SA, Zhuravlev YN., 2008. Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli. Biotechnol Bioeng. 100(1):118-25. Sinnott ML., 1990. Catalytic mechanisms of enzymic glycosyl transfer. Chem Rev. 90, 1171-202. Slightom JL, Durandtardif M, Jouanin L, Tepfer D., 1986. Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. J Biol Chem. 261(1):108-21. Spena A, Aalen RB, Schulze SC., 1989. Cell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: a visual assay for transposon excision in transgenic plants. Plant Cell. 1(12):1157-64. Spena A, Schmulling T, Koncz C, Schell JS., 1987. Independent and synergistic activity of rol A, B and C loci in stimulating abnormal growth in plants. EMBO J. 6(13):3891-9. Srivastava V, Kaur R, Chattopadhyay SK, Banerjee S., 2013. Production of industrially important cosmaceutical and pharmaceutical derivatives of betuligenol by Atropa belladonna hairy root mediated biotransformation. Ind Crops Prod. 44, 171-5. Tepfer D., 1984. Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype. Cell. 37(3):959-67. Veena V, Taylor CG., 2007. Agrobacterium rhizogenes: recent developments and promising applications. In Vitro Cell Dev Biol. 43, 383-403. Vervliet G, Holsters M, Teuchy H, Vanmontagu M, Schell J., 1975. Characterization of different plaque-forming and defective temperate phages in Agrobacterium. J Gen Virol. 26(1):33-48. Wang JH, Lin HH, Liu CT, Lin TC, Liu LY, Lee KT., 2014. Transcriptomic analysis reveals that reactive oxygen species and genes encoding lipid transfer protein are associated with tobacco hairy root growth and branch development. Mol Plant Microbe Interact. 27(7):678-87. Weising K, Kahl G., 1996. Natural genetic engineering of plant cells: the molecular biology of crown gall and hairy root disease. World J Microbiol Biotechnol. 12(4):327-51. White FF, Taylor BH, Huffman GA, Gordon MP, Nester EW., 1985. Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol. 164(1):33-44. White LO., 1972. The Taxonomy of the Crown-gall Organism Agrobaterium tumefaciens and Its Relationship to Rhizobia and Other Agrobacteria. J Gen Microbiol. 72: 565-74. Xu B, Ohtani M, Yamaguchi M, Toyooka K, Wakazaki M, Sato M, Kubo M, Nakano Y, Sano R, Hiwatashi Y, Murata T, Kurata T, Yoneda A, Kato K, Hasebe M, Demura T., 2014. Contribution of NAC transcription factors to plant adaptation to land. Science. 343(6178):1505-8. Yoo SD, Cho YH, Sheen J., 2007. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nat Protoc. 2(7):1565-72. Zuker A, Tzfira T, Scovel G, Ovadis M, Shklarman E, Itzhaki H, Vainstein A., 2001. RolC-Transgenic Carnation with Improved Horticultural Traits: Quantitative and Qualitative Analyses of Greenhouse-grown Plants. J Amr Soc Hort Sci. 126, 13-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55480 | - |
dc.description.abstract | 毛狀根是植物受到根毛農桿菌 (Agrobacterium rhizogenes) 感染所誘導產生的轉形根組織。先前研究中指出誘根質體 (Ri plasmid) 上的 rol 基因群在誘發毛狀根的過程中扮演重要的角色;其中 rolC 基因又被報導與毛狀根快速生長及側根發育有關。本實驗室先前研究顯示:缺失 rolC 的菸草毛狀根生長能力明顯下降,且 rolC 對毛狀根的發育及其快速生長的特性密切相關,但目前對於 rolC 如何調控毛狀根的形成與其特性仍屬未知。同時,阿拉伯芥中已有許多轉錄因子對於細胞、組織的生長有著全面性調控之報導。綜合以上,本研究針對阿拉伯芥中可能受到 rolC 調控的轉錄因子進行功能性探討,以推測 rolC 基因影響毛狀根組織生成及發育的機制。根據先前基因微陣列 (microarray) 分析結果,本研究以阿拉伯芥基因缺陷株的主根長度為參數,挑選出一個可能為轉錄因子的 NAC13 蛋白質。我們先在酵母菌的系統中,利用轉錄分析法 (transactivation assay) 證明 NAC13 具有轉錄下游基因的能力;再將 NAC13 與黃色螢光蛋白質融合連接後,利用共軛焦顯微鏡發現 NAC13 蛋白質在細胞內的表現位置是在細胞核中及其周圍。同時,藉由比較缺失 nac13 基因的阿拉伯芥毛狀根與野生形毛狀根的外觀、誘導率及粒線體含量,推測 rolC可能透過 nac13 來調控下游 aox1a 及 hre2 基因的表現,降低細胞中的活性含氧物質,以阻止粒線體的凋亡及維持毛狀根快速生長。 | zh_TW |
dc.description.abstract | Hairy root is a specialized, fast-growing tissue that is induced by the infection of Agrobacterium rhizogenes. Root locus genes (rol genes) which encoded in root-inducing plasmid (Ri plasmid) of A. rhizogenes play key roles in hairy root formation. Among the rol genes, researches showed that fast and branched root growth required the presence of rolC gene. Our previous data showed that the viability of rolC-deficient hairy roots decreased significantly compared to wild-type hairy roots, hairy root formation and its fast growth were regulated by rolC with unknown mechanism. Previous researches also indicated that many transcription factors were thought to have large impacts on tissue characteristics in Arabidopsis. Took the things together, we focused on revealing the transcription factors which under the regulation of rolC, attempting to elucidate the mechanism of hairy root formation. Based on the previous microarray results, a putative transcription factor, NAC13, was identified according to its capacity for regulating the main root length in Arabidopsis mutants. The activation ability of NAC13 was demonstrated by using transactivation assay in yeast. By confocal microscopy observation, fusion proteins of NAC13 and yellow fluorescence protein (NAC13-YFP) were visualized in and around the nucleus. Moreover, comparing the morphology, induction efficiency and the mitochondria of nac13-deficient hairy roots and wild-type hairy roots showed that rolC might involve in the mitochondria stress signaling pathway through nac13 gene and its downstream genes, aox1a and hre2, to eliminate the excess reactive oxygen species for maintaining fast growth and preventing mitochondrial apoptosis in hairy roots. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T04:04:51Z (GMT). No. of bitstreams: 1 ntu-103-R01b22018-1.pdf: 1308042 bytes, checksum: f6754ad3c2cbf1b77a262b695a493bc5 (MD5) Previous issue date: 2014 | en |
dc.description.tableofcontents | 謝誌 i
中文摘要 ii Abstract iii Contents iv Contents of tables and figures vii Chapter I Introduction 1 1.1 Applications of hairy roots 2 1.2 Hairy roots are formed by infections of Agrobacterium rhizogenes 3 1.3 RolC is essential in hairy root formation 4 1.4 NAC13 is a putative transcription factor 6 1.5 Aim of this study 7 Chapter II Materials and Methods 8 2.1 Biological materials and growth conditions 9 2.1.1 Arabidopsis thaliana Col-0 wild-type and mutants 9 2.1.2 Nicotiana tabacum cultivar Bright Yellow-2 (BY-2) 9 2.1.3 Agrobacterium rhizogenes A4 9 2.1.4 Saccharomyces cerevisiae Y2HGold 10 2.1.4.1 Growth conditions 10 2.1.4.2 Preparation of S. cerevisiae Y2HGold competent cells 10 2.2 Plasmid construction 11 2.2.1 Constructs of Gateway entry vectors 11 2.2.2 Constructs of Gateway destination vectors 11 2.3 Protoplasts isolation 12 2.3.1 A. thaliana Col-0 mesophyll protoplasts 12 2.3.2 N. tabacum BY-2 protoplasts 12 2.4 Microarray data analysis, sequence alignment and gene ontology 13 2.5 Genotype identification of Arabidopsis mutants 13 2.6 Measurement of root length and seed germination rate 14 2.7 A. rhizogenes-mediated transformation 14 2.8 Determination of protein subcellular localization 14 2.9 Transactivation activity assays in S. cerevisiae 15 2.10 Mitochondria staining of Arabidopsis intact roots and hairy roots 16 Chapter III Results 17 3.1 Microarray data analysis, sequence alignment and gene ontology 18 3.2 Genotype identification of Arabidopsis mutants 18 3.3 Measurement of root length and seed germination rate 19 3.4 Protein subcellular localization of RolC 19 3.5 Protein subcellular localization of NAC13 20 3.6 Transactivation activity assays in S. cerevisiae 21 3.7 The induction efficiency of nac13-deficent hairy roots 21 3.8 Mitochondria staining of Arabidopsis intact roots and hairy roots 22 Chapter IV Discussion 23 4.1 Select candidate genes from microarray of N. tabacum hairy roots 24 4.2 Growing index of Arabidopsis mutants 25 4.3 NAC13 is a putative transcription factor 25 4.3.1 Subcellular localization of NAC13 26 4.3.2 Activation activity of NAC13 26 4.4 The induction efficiency of nac13-deficent hairy roots 27 4.5 The number of mitochondria might be regulated by NAC13 27 4.6 Possible regulatory mechanism of NAC13 in Arabidopsis 28 4.7 Possible regulatory mechanism of RolC in Arabidopsis hairy roots 30 Conclusions 32 Tables and Figures 34 References 52 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥毛狀根形成相關之轉錄因子 NAC13 的功能性分析 | zh_TW |
dc.title | Functional Analysis of Transcription Factor NAC13 Involved in Hairy Root Formation in Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉啟德(Chi-Te Liu),鄭石通(Shih-Tong Jeng),黃鵬林(Pung-Ling Huang),楊健志(Chien-Chih Yang) | |
dc.subject.keyword | 阿拉伯芥,毛狀根,rolC,NAC13,粒線體, | zh_TW |
dc.subject.keyword | Arabidopsis,hairy roots,rolC,transcription factors,NAC13,mitochondria, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2014-09-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-103-1.pdf 目前未授權公開取用 | 1.28 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。