Please use this identifier to cite or link to this item:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55193
Full metadata record
???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
---|---|---|
dc.contributor.advisor | 張麗冠(Li-Kwan Chang) | |
dc.contributor.author | Pei-Si Cai | en |
dc.contributor.author | 蔡沛賜 | zh_TW |
dc.date.accessioned | 2021-06-16T03:50:46Z | - |
dc.date.available | 2020-03-13 | |
dc.date.copyright | 2015-03-13 | |
dc.date.issued | 2015 | |
dc.date.submitted | 2015-01-21 | |
dc.identifier.citation | Bernhard W, Barreto K, Raithatha S, Sadowski I (2013) An upstream YY1 binding site on the HIV-1 LTR contributes to latent infection. PLoS One 8: e77052
Blissard GW, Kogan PH, Wei R, Rohrmann GF (1992) A synthetic early promoter from a baculovirus: roles of the TATA box and conserved start site CAGT sequence in basal levels of transcription. Virology 190: 783-793 Bonnet MC, Weil R, Dam E, Hovanessian AG, Meurs EF (2000) PKR stimulates NF-kappaB irrespective of its kinase function by interacting with the IkappaB kinase complex. Mol Cell Biol 20: 4532-4542 Bushmeyer S, Park K, Atchison ML (1995) Characterization of functional domains within the multifunctional transcription factor, YY1. J Biol Chem 270: 30213-30220 Chang LK, Huang PH, Shen WT, Yang SH, Liu WJ, Lo CF (2012) Role of Penaeus monodon Kruppel-like factor (PmKLF) in infection by white spot syndrome virus. Dev Comp Immunol 36: 121-129 Chang PJ, Chen LW, Shih YC, Tsai PH, Liu AC, Hung CH, Liou JY, Wang SS (2011) Role of the cellular transcription factor YY1 in the latent-lytic switch of Kaposi's sarcoma-associated herpesvirus. Virology 413: 194-204 Chen AJ, Gao L, Wang XW, Zhao XF, Wang JX (2013) SUMO-conjugating enzyme E2 UBC9 mediates viral immediate-early protein SUMOylation in crayfish to facilitate reproduction of white spot syndrome virus. J Virol 87: 636-647 Chen JC, Liu PC, Lin YT. (1988) Culture of Penaeus monodon in an intensified system in Taiwan. Aquaculture 77: 319-328 Chen LL, Lo CF, Chiu YL, Chang CF, Kou GH (2000) Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata. Dis Aquat Organ 40: 157-161 Chen YH, Jia XT, Huang XD, Zhang S, Li M, Xie JF, Weng SP, He JG (2011) Two Litopenaeus vannamei HMGB proteins interact with transcription factors LvSTAT and LvDorsal to activate the promoter of white spot syndrome virus immediate-early gene ie1. Mol Immunol 48: 793-799 Choi YS, Sinha S (2006) Determination of the consensus DNA-binding sequence and a transcriptional activation domain for ESE-2. Biochem J 398: 497-507 Chou HY, Huang CY, Wang CH, Chiang HC, Lo CF (1995) Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis Aquat Organ 23: 165-173 Coull JJ, Romerio F, Sun JM, Volker JL, Galvin KM, Davie JR, Shi Y, Hansen U, Margolis DM (2000) The human factors YY1 and LSF repress the human immunodeficiency virus type 1 long terminal repeat via recruitment of histone deacetylase 1. J Virol 74: 6790-6799 Everett RD (2014) HSV-1 biology and life cycle. Methods Mol Biol 1144: 1-17 Gordon S, Akopyan G, Garban H, Bonavida B (2006) Transcription factor YY1: structure, function, and therapeutic implications in cancer biology. Oncogene 25: 1125-1142 Gualberto A, LePage D, Pons G, Mader SL, Park K, Atchison ML, Walsh K (1992) Functional antagonism between YY1 and the serum response factor. Mol Cell Biol 12: 4209-4214 Hause LL, McIver KS (2012) Nucleotides critical for the interaction of the Streptococcus pyogenes Mga virulence regulator with Mga-regulated promoter sequences. J Bacteriol 194: 4904-4919 Hergovich A, Stegert MR, Schmitz D, Hemmings BA (2006) NDR kinases regulate essential cell processes from yeast to humans. Nat Rev Mol Cell Bio 7: 253-264 Ho J, Benchimol S (2003) Transcriptional repression mediated by the p53 tumour suppressor. Cell Death Differ 10: 404-408 Huang PS (2005) An economic analysis of pacific white shrimp (Litopenaeus vannamei) aquaculture in Taiwan. 臺灣海洋大學應用經濟研究所碩士論文 Huang XD, Zhao L, Zhang HQ, Xu XP, Jia XT, Chen YH, Wang PH, Weng SP, Yu XQ, Yin ZX, He JG (2010) Shrimp NF-kappaB binds to the immediate-early gene ie1 promoter of white spot syndrome virus and upregulates its activity. Virology 406: 176-180 Hyde-DeRuyscher RP, Jennings E, Shenk T (1995) DNA binding sites for the transcriptional activator/repressor YY1. Nucleic Acids Res 23: 4457-4465 Hydederuyscher RP, Jennings E, Shenk T (1995) DNA-binding sites for the transcriptional activator/repressor YY1. Nucleic Acids Res 23: 4457-4465 Kasornchandra J, Boonyaratpalin S, Itami T (1998) Detection of white-spot syndrome in cultured penaeid shrimp in Asia: Microscopic observation and polymerase chain reaction. Aquaculture 164: 243-251 Kim J, Kim J (2009) YY1's longer DNA-binding motifs. Genomics 93: 152-158 Kim JD, Faulk C, Kim J (2007) Retroposition and evolution of the DNA-binding motifs of YY1, YY2 and REX1. Nucleic Acids Res 35: 3442-3452 Lee TC, Shi Y, Schwartz RJ (1992) Displacement of BrdUrd-induced YY1 by serum response factor activates skeletal alpha-actin transcription in embryonic myoblasts. Proc Natl Acad Sci U S A 89: 9814-9818 Leu JH, Yang F, Zhang X, Xu X, Kou GH, Lo CF (2009) Whispovirus. Curr Top Microbiol Immunol 328: 197-227 Li F, Li MY, Ke W, Ji YC, Bian XF, Yan XM (2009) Identification of the immediate-early genes of white spot syndrome virus. Virology 385: 267-274 Li M, Baumeister P, Roy B, Phan T, Foti D, Luo S, Lee AS (2000) ATF6 as a transcription activator of the endoplasmic reticulum stress element: thapsigargin stress-induced changes and synergistic interactions with NF-Y and YY1. Mol Cell Biol 20: 5096-5106 Liao IC (1989) Peneaus monodon culture in Taiwan: through two decades of growth. Int J Aquacult FISH Technol 1: 16-24 Liao IC, Huang TL, Katsutani K (1969) A preliminary report on artificial propagation of Penaeus monodon. Jt Comm Rural Reconstr Fish Ser 8: 67-71 Lin F, Huang H, Ke W, Hou L, Li F, Yang F (2013) Characterization of white spot syndrome virus immediate-early gene promoters. J Gen Virol 94: 387-392 Lin F, Huang H, Xu L, Li F, Yang F (2011) Identification of three immediate-early genes of white spot syndrome virus. Arch Virol 156: 1611-1614 Lin YR, Hung HC, Leu JH, Wang HC, Kou GH, Lo CF (2011) The role of aldehyde dehydrogenase and hsp70 in suppression of white spot syndrome virus replication at high temperature. J Virol 85: 3517-3525 Liu WJ, Chang YS, Wang AH, Kou GH, Lo CF (2007) White spot syndrome virus annexes a shrimp STAT to enhance expression of the immediate-early gene ie1. J Virol 81: 1461-1471 Liu WJ, Lo CF, Kou GH, Leu JH, Lai YJ, Chang LK, Chang YS (2015) The promoter of the white spot syndrome virus immediate-early gene WSSV108 is activated by the cellular KLF transcription factor. Dev Comp Immunol 49: 7-18 Lo CF, Ho CH, Peng SE, Chen CH, Hsu HC, Chiu YL, Chang CF, Liu KF, Su MS, Wang CH, Kou GH (1996a) White spot syndrome baculovirus (WSBV) detected in cultured and captured shrimp, crabs and other arthropods. Dis Aquat Organ 27: 215-225 Lo CF, Hsu HC, Tsai MF, Ho CH, Peng SE, Kou GH, Lightner DV (1999) Specific genomic DNA fragment analysis of different geographical clinical samples of shrimp white spot syndrome virus. Dis Aquat Organ 35: 175-185 Lo CF, Kou GH (1998) Virus-associated white spot syndrome of shrimp in Taiwan: A review. Fish Pathol 33: 365-371 Lo CF, Leu JH, Ho CH, Chen CH, Peng SE, Chen YT, Chou CM, Yeh PY, Huang CJ, Chou HY, Wang CH, Kou GH (1996b) Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction. Dis Aquat Organ 25: 133-141 Lu CP, Zhu S, Guo FS, Wu SY (1997) Electron microscopic observation on a non-occluded baculo-like virus in shrimps. Arch Virol 142: 2073-2078 Mohareer K, Sahdev S, Hasnain SE (2011) Baculovirus p35 gene is oppositely regulated by P53 and AP-1 like factors in Spodoptera frugiperda. Biochem Biophys Res Commun 414: 688-693 Momoeda M, Kawase M, Jane SM, Miyamura K, Young NS, Kajigaya S (1994) The transcriptional regulator YY1 binds to the 5'-terminal region of B19 parvovirus and regulates P6 promoter activity. J Virol 68: 7159-7168 Montalvo EA, Cottam M, Hill S, Wang YJ (1995) YY1 binds to and regulates cis-acting negative elements in the Epstein-Barr virus BZLF1 promoter. J Virol 69: 4158-4165 Nagawa F, Ishiguro K, Tsuboi A, Yoshida T, Ishikawa A, Takemori T, Otsuka AJ, Sakano H (1998) Footprint analysis of the RAG protein recombination signal sequence complex for V(D)J type recombination. Mol Cell Biol 18: 655-663 Ongvarrasopone C, Chanasakulniyom M, Sritunyalucksana K, Panyim S (2008) Suppression of PmRab7 by dsRNA inhibits WSSV or YHV infection in shrimp. Mar Biotechnol 10: 374-381 Pavletich NP, Pabo CO (1993) Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers. Science 261: 1701-1707 Perkins ND, Edwards NL, Duckett CS, Agranoff AB, Schmid RM, Nabel GJ (1993) A cooperative interaction between NF-kappa B and Sp1 is required for HIV-1 enhancer activation. EMBO J 12: 3551-3558 Raich N, Clegg CH, Grofti J, Romeo PH, Stamatoyannopoulos G (1995) GATA1 and YY1 are developmental repressors of the human epsilon-globin gene. EMBO J 14: 801-809 Sanchez-Paz A (2010) White spot syndrome virus: an overview on an emergent concern. Vet Res 41: 43 Sarathi M, Simon MC, Ahmed VP, Kumar SR, Hameed AS (2008) Silencing VP28 gene of white spot syndrome virus of shrimp by bacterially expressed dsRNA. Mar Biotechnol 10: 198-206 Sepulveda MA, Emelyanov AV, Birshtein BK (2004) NF-kappa B and Oct-2 synergize to activate the human 3'Igh Hs4 enhancer in B cells. J Immunol 172: 1054-1064 Seto E, Shi Y, Shenk T (1991) YY1 is an initiator sequence-binding protein that directs and activates transcription in vitro. Nature 354: 241-245 Shi Y, Lee JS, Galvin KM (1997) Everything you have ever wanted to know about Yin Yang 1. BBA-Rev Cancer 1332: 49-66 Shi Y, Seto E, Chang LS, Shenk T (1991) Transcriptional repression by YY1, a human GLI-Kruppel-related protein, and relief of repression by adenovirus E1A protein. Cell 67: 377-388 Shrivastava A, Calame K (1994) An analysis of genes regulated by the multi-functional transcriptional regulator Yin Yang-1. Nucleic Acids Res 22: 5151-5155 Shrivastava A, Saleque S, Kalpana GV, Artandi S, Goff SP, Calame K (1993) Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc. Science 262: 1889-1892 Sui GC, El Bachir A, Shi YJ, Brignone C, Wall NR, Yin P, Donohoe M, Luke MP, Calvo D, Grossman SR, Shi Y (2004) Yin Yang 1 is a negative regulator of p53. Cell 117: 859-872 Suzuki M, Yagi N (1994) DNA recognition code of transcription factors in the helix-turn-helix, probe helix, hormone receptor, and zinc finger families. Proc Natl Acad Sci U S A 91: 12357-12361 Tsai MF, Kou GH, Liu HC, Liu KF, Chang CF, Peng SE, Hsu HC, Wang CH, Lo CF (1999) Long-term presence of white spot syndrome virus (WSSV) in a cultivated shrimp population without disease outbreaks Dis Aquat Organ 38: 107-114 Usheva A, Shenk T (1994) TATA-binding protein-independent initiation - YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA. Cell 76: 1115-1121 Van Hulten MC, Witteveldt J, Peters S, Kloosterboer N, Tarchini R, Fiers M, Sandbrink H, Lankhorst RK, Vlak JM (2001) The white spot syndrome virus DNA genome sequence. Virology 286: 7-22 Wang CH, Lo CF, Leu JH, Chou CM, Yeh PY, Chou HY, Tung MC, Chang CF, Su MS, Kou GH (1995) Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis Aquat Organ 23: 239-242 Wang PH, Gu ZH, Wan DH, Liu BD, Huang XD, Weng SP, Yu XQ, He JG (2013) The shrimp IKK-NF-kappa B signaling pathway regulates antimicrobial peptide expression and may be subverted by white spot syndrome virus to facilitate viral gene expression. Cell Mol Immunol 10: 423-436 Wang ZL, Chua HK, Gusti AARA, He F, Fenner B, Manopo I, Wang H, Kwang J (2005) RING-H2 protein WSSV249 from white spot syndrome virus sequesters a shrimp ubiquitin-conjugating enzyme, PvUbc, for viral pathogenesis. J Virol 79: 8764-8772 Wu J, Lin Q, Lim TK, Liu T, Hew CL (2007) White spot syndrome virus proteins and differentially expressed host proteins identified in shrimp epithelium by shotgun proteomics and cleavable isotope-coded affinity tag. J Virol 81: 11681-11689 Yang F, He J, Lin X, Li Q, Pan D, Zhang X, Xu X (2001) Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75: 11811-11820 Yant SR, Zhu W, Millinoff D, Slightom JL, Goodman M, Gumucio DL (1995) High affinity YY1 binding motifs: identification of two core types (ACAT and CCAT) and distribution of potential binding sites within the human beta globin cluster. Nucleic Acids Res 23: 4353-4362 Yao YL, Yang WM, Seto E (2001) Regulation of transcription factor YY1 by acetylation and deacetylation. Mol Cell Biol 21: 5979-5991 Zalani S, Coppage A, Holley-Guthrie E, Kenney S (1997) The cellular YY1 transcription factor binds a cis-acting, negatively regulating element in the Epstein-Barr virus BRLF1 promoter. J Virol 71: 3268-3274 Zamanian-Daryoush M, Mogensen TH, DiDonato JA, Williams BR (2000) NF-kappaB activation by double-stranded-RNA-activated protein kinase (PKR) is mediated through NF-kappaB-inducing kinase and IkappaB kinase. Mol Cell Biol 20: 1278-1290 Zhu F, Du HH, Miao ZG, Quan HZ, Xu ZR (2009) Protection of Procambarus clarkii against white spot syndrome virus using inactivated WSSV. Fish shellfish Immunol 26: 685-690 黃庭儀 (2014) Regulation of the wssv126 (ie1) of white spot syndrome virus by Litopenaeus vannamei YY1. 臺灣大學生化科技研究所碩士論文 楊舒涵 (2011) Role of the Krupple-like factor in Litopenaeus vannamei after white spot syndrome virus infection. 臺灣大學生化科技研究所碩士論文 薛月順 (2010) 臺灣「草蝦王國」的形成(1968-1988)—政府與民間扮演的角色. 國史館館刊 24: 139-176 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/55193 | - |
dc.description.abstract | 白點症病毒 (White spot syndrome virus) 為大型雙股DNA病毒,對於對蝦類 宿主 (Penaeidea) 具有高致病性。目前白點症病毒已被報導具有21個極早期基因,其中極早期基因wssv108的胺基酸序列與Bad/Rrf2家族轉錄因子具有25%之相似性。此外,前人研究顯示白點症病毒會挾持蝦宿主轉錄因子活化其極早期基因進而走向病毒溶裂期。而本研究室先前自白蝦mRNA中選殖出一Yin-yang 1 (YY1)相似基因LvYY1,YY1為廣泛表現於各種組織之轉錄調控因子,在各種物種中已被報導能調控數種病毒極早期基因,因此本研究假設白蝦基因LvYY1會參與白點症病毒極早期基因的調控。結果發現LvYY1靜默化能夠抑制白點症病毒複製數以及延遲病毒感染白蝦的死亡率,而luciferase reporter assay分析顯示LvYY1在Drosophila Schneider 2昆蟲細胞株中能促進白點症病毒極早基因wssv108啟動子的轉錄活性至3.9倍,且啟動子截切分析 (promoter deletion analysis) 顯示wssv108啟動子上的30-mer DNA序列 (-102 nt/-73 nt) 為LvYY1的可能調控區段。但是經由線上資料庫的序列比對,此DNA調控區並不具有YY1家族轉錄因子之保守性DNA結合序列,因此製備分段競爭者序列以及位點突變競爭者序列,再以electrophoretic mobility shift assay證實wssv108轉錄起始點上游序列第91到83個核苷酸之間的序列為LvYY1的結合位,並以LvYY1結合位突變株證明LvYY1是藉由此段序列活化wssv108的啟動子。最後發現當白蝦體內LvYY1的表現量受到抑制時,也會導致病毒的wssv108表現量隨之下降。總結以上,本研究證實白蝦的LvYY1經由非典型的YY1的DNA結合序列促進白點症病毒極早期基因wssv108的轉錄活性,其機轉可能與白點症病毒的致病相關。 | zh_TW |
dc.description.abstract | White spot syndrome virus (WSSV) is a deadly pathogen that causes shrimp death with a mortality rate of 90-100%. So far, twenty-one WSSV immediate-early genes (IE genes) had been found. The protein sequence of the wssv108 IE gene displays 25% identify with Bad/Rrf2 family transcription factor. Previous studies showed that WSSV could annex shrimp transcription factors to activate its own immediate-early genes to get into lytic phase. Recently, LvYY1 (Yin-yang 1) was cloned from Litopenaeus vannamei. A ubiquitously distributed transcription factor YY1 was known to regulate viral immediate-early genes. This study assumed that LvYY1 participates in the pathogenesis of WSSV. Firstly, knockdown of LvYY1 expression in WSSV-infected shrimp caused lower copy numbers of WSSV and delayed cumulative mortality. Moreover, the reporter assay revealed that the promoter activity of wssv108 was activated 3.9 fold by LvYY1 in S2 cell. Deletion analysis of the promoter suggested that 30-mer DNA region (-102 nt/-73 nt) is important to the activation. According to the sequence alignment, this 30-mer DNA region in the wssv108 promoter has no consensus sequence of YY1 binding sites. The electrophoretic mobility shift assay using mutant sequences as competitive oligonucleotides confirmed that recombinant LvYY1 from Sf9 insect cell binds on the sequence from -91 nt to -83 nt of the wssv108 promoter. Furthermore, site-directed mutagenesis of the promoter verified the YY1 binding site is crucial for transcriptional regulation of the wssv108 promoter by LvYY1. Finally, silencing of LvYY1 expression in WSSV-infected shrimp reduced the levels of wssv108 mRNA. Therefore, this study demonstrated that LvYY1 enhances the transactivation activity of wssv108 via an atypical YY1-binding site to affect WSSV infection. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T03:50:46Z (GMT). No. of bitstreams: 1 ntu-104-R01b22034-1.pdf: 12451544 bytes, checksum: 2f2bbc779d037c1a1059f8d4c0d48015 (MD5) Previous issue date: 2015 | en |
dc.description.tableofcontents | 第一章 前言 1
1. 臺灣養殖蝦業的發展概況 1 2. 白點症病毒 (white spot syndrome virus; WSSV) 2 3. 白點症病毒的生活史 4 4. 白點症病毒的極早期基因 5 5. 宿主轉錄因子與白點症病毒極早期基因的調控 6 6. YY1家族轉錄因子 7 7. YY1家族蛋白質對病毒極早期基因的調控 10 第二章 材料和方法 12 1. 菌種、細胞株、實驗動物以及白點症病毒液 12 2. 質體建構 12 3. 質體DNA萃取 13 4. 勝任細胞的製備 14 5. 細胞轉型 14 6. 雙股 RNA 製備 14 7. 白點症病毒感染及白蝦死亡率觀察 15 8. 病毒感染白蝦的病毒複製數檢測 16 9. 冷光活性分析 (Luciferase reporter assay) 16 10. 5’端快速擴增cDNA端點技術 (5’ rapid amplification of cDNA ends) 17 11. LvYY1於Sf9昆蟲細胞之暫時性表現 18 12. 昆蟲細胞核蛋白質的萃取 18 13. 電泳游動性轉移分析 (Electrophoretic mobility shift assay; EMSA) 19 14. 病毒感染蝦隻之定量反轉錄PCR (Quantitative reverse transcription PCR) 19 第三章 結果 21 1. LvYY1基因靜默化會抑制白點症病毒複製以及感染白蝦的死亡率 21 2. LvYY1會調控白點症病毒極早期基因的轉錄活性 22 3. wssv108啟動子上的30-mer片段為LvYY1的調控序列 22 4. wssv108啟動子上的9-mer片段為LvYY1的DNA結合位 23 4.1 LvYY1蛋白質結合於wssv108啟動子 23 4.2 以分段競爭者核苷酸序列縮小LvYY1的DNA結合位至20-mer片段 24 4.3 以多點核苷酸突變序列縮小LvYY1的DNA結合位至12-mer片段 25 4.4 以單點核苷酸突變確認9-mer片段為LvYY1的DNA結合位 26 4.5 LvYY1與wssv108啟動子的9-mer片段之結合專一性 27 5. LvYY1藉由其DNA結合位調控wssv108的轉錄活性 27 6. LvYY1基因靜默化導致wssv108表現量下降 28 第四章 討論 29 第五章 圖表 37 表1、本研究所使用的引子列表 37 圖1、LvYY1基因靜默化對於感染白點症病毒的白蝦死亡程度之影響 41 圖2、LvYY1靜默化影響感染白蝦的白點症病毒複製數的時序變化 42 圖3、LvYY1對病毒極早期基因啟動子轉錄活性之影響 43 圖4、極早期基因wssv108的轉錄起始點位置示意圖 44 圖5、LvYY1對極早期基因wssv108啟動子片段轉錄活性的影響 45 圖6、以分段競爭者序列進行LvYY1的DNA結合區域分析 46 圖7、以多點突變競爭者序列進行LvYY1蛋白質的DNA結合區域分析 47 圖8、採用單點核苷酸突變推進策略進行DNA結合區域分析 48 圖9、LvYY1與wssv108啟動子的20-mer片段之間的專一性結合 49 圖10、LvYY1結合位突變對LvYY1調控wssv108啟動子的影響 50 圖11、LvYY1基因靜默化對wssv108表現量的影響 51 參考文獻 52 附錄 61 | |
dc.language.iso | zh-TW | |
dc.title | 白蝦 YY1 調控白點症病毒極早期基因 wssv108 的機轉 | zh_TW |
dc.title | Regulation of the immediate-early gene wssv108 of white spot syndrome virus by Litopenaeus vannamei Yin-yang 1 | en |
dc.type | Thesis | |
dc.date.schoolyear | 103-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃偉邦(Wei-Pang Huang),王涵青(Han-Ching Wang),劉宛菁(Wang-Jing Liu),呂健宏(Jiann-Horng Leu) | |
dc.subject.keyword | 白點症病毒,白蝦,Yin-yang 1 蛋白質,極早期基因,wssv108基因, | zh_TW |
dc.subject.keyword | white spot syndrome virus,Litopenaeus vannamei,Yin-yang 1,immediate-early gene,wssv108, | en |
dc.relation.page | 67 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2015-01-21 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
Appears in Collections: | 生化科技學系 |
Files in This Item:
File | Size | Format | |
---|---|---|---|
ntu-104-1.pdf Restricted Access | 12.16 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.