請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49702
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄧述諄(Shu-Chun Teng) | |
dc.contributor.author | Hsuan-Ming Chen | en |
dc.contributor.author | 陳宣銘 | zh_TW |
dc.date.accessioned | 2021-06-15T11:42:55Z | - |
dc.date.available | 2021-08-26 | |
dc.date.copyright | 2016-08-26 | |
dc.date.issued | 2016 | |
dc.date.submitted | 2016-08-15 | |
dc.identifier.citation | Amberg, D. C., et al. (2005). 'Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, 2005 Edition (Cold Spring).'
Ashburner, M., Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G (2000). 'Gene ontology: tool for the unification of biology. The Gene Ontology Consortium.' Nature genetics 25(1): 25-49. Bodenmiller, B., et al. (2010). 'Phosphoproteomic analysis reveals interconnected system-wide responses to perturbations of kinases and phosphatases in yeast.' Sci Signal 3(153): rs4. Busti, S., et al. (2010). 'Glucose signaling-mediated coordination of cell growth and cell cycle in Saccharomyces cerevisiae.' Sensors (Basel) 10(6): 6195-6240. Caspeta, L., et al. (2015). 'Modifying Yeast Tolerance to Inhibitory Conditions of Ethanol Production Processes.' Front Bioeng Biotechnol 3: 184. Choi, K. M., et al. (2013). 'Characterization of global gene expression during assurance of lifespan extension by caloric restriction in budding yeast.' Exp Gerontol 48(12): 1455-1468. Conrad, M., et al. (2014). 'Nutrient sensing and signaling in the yeast Saccharomyces cerevisiae.' FEMS Microbiol Rev 38(2): 254-299. de Cabo, R., Carmona-Gutierrez D, Bernier M, Hall MN, Madeo F (2014). 'The Search for Antiaging Interventions: From Elixirs to Fasting Regimens.' Cell 157(7): 1515–1526. Dennis G Jr1, S. B., Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA (2003). 'DAVID: Database for Annotation, Visualization, and Integrated Discovery.' Genome Biol 4(5): 3. Distel, B., Gould, SJ., Voorn-Brouwer, T., van der Berg, M., Tabak, HF., Subramani, S. (1992). 'The carboxyl-terminal tripeptide serine-lysine-leucine of firefly luciferase is necessary but not sufficient for peroxisomal import in yeast.' New Biol. 4(2): 157-165. Donzé, O., Picard, D. (1999). 'Hsp90 binds and regulates Gcn2, the ligand-inducible kinase of the alpha subunit of eukaryotic translation initiation factor 2 ' Mol Biol Cell 19(12): 8422-8432. Duina, A. A., et al. (1998). 'Requirement for Hsp90 and a CyP-40-type cyclophilin in negative regulation of the heat shock response.' J Biol Chem 273(30): 18974-18978. Fabrizio, P. and V. D. Longo (2003). 'The chronological life span of Saccharomyces cerevisiae.' Aging Cell 2(2): 73-81. Fabrizio, P., et al. (2004). 'Chronological aging-independent replicative life span regulation by Msn2/Msn4 and Sod2 in Saccharomyces cerevisiae.' FEBS Lett 557(1-3): 136-142. Fabrizio, P., et al. (2001). 'Regulation of longevity and stress resistance by Sch9 in yeast.' Science 292(5515): 288-290. Fink, L. S. R. a. G. R. (1998). 'The three yeast A kinases have specific signaling functions in pseudohyphal growth.' Proc Natl Acad Sci U S A 95(23): 13783-13787. Fontana, L., et al. (2010). 'Extending healthy life span--from yeast to humans.' Science 328(5976): 321-326. Franzosa EA, A. V., Frydman J, Xia Y, McClellan AJ (2011). 'Heterozygous yeast deletion collection screens reveal essential targets of Hsp90.' PLOS one 6(11). Fujioka, Y., et al. (2014). 'Structural basis of starvation-induced assembly of the autophagy initiation complex.' Nat Struct Mol Biol 21(6): 513-521. Gil, F. R., Sanz, P., Entian, K.D., and Jose Antonio Prieto (1998). 'Carbon Source-Dependent Phosphorylation of Hexokinase PII and Its Role in the Glucose-Signaling Response in Yeast.' Mol. Cell. Biol 18 (5): 2940-2948. Han, C. L., et al. (2008). 'A multiplexed quantitative strategy for membrane proteomics: opportunities for mining therapeutic targets for autosomal dominant polycystic kidney disease.' Mol Cell Proteomics 7(10): 1983-1997. Hansen, M., et al. (2008). 'A role for autophagy in the extension of lifespan by dietary restriction in C. elegans.' PLoS Genet 4(2): e24. Hansen, M., Chandra, A., Mitic, L.L., Onken, B., Driscoll, M., Kenyon, C. (2009). 'A role for autophagy in the extension of lifespan by dietary restriction in C. elegans.' PLOS Genetics 4(2): e24. Harmen, D. (1981). 'The aging process.' Proc Natl Acad Sci U S A. 78(11): 7124-7128. Hawle, P., Horst, D., Bebelman, JP., Yang, XX., Siderius, M., van der Vies, SM. (2007). 'Cdc37p is required for stress-induced high-osmolarity glycerol and protein kinase C mitogen-activated protein kinase pathway functionality by interaction with Hog1p and Slt2p (Mpk1p).' Eukaryot Cell 6(3): 521-532. Hughes, A. L. and D. E. Gottschling (2012). 'An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast.' Nature 492(7428): 261-265. Imai, S., Armstrong, C.M., Kaeberlein, M., & Leonard Guarente (2000). 'Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase.' Nature 403: 795-800. Jazwinski, S. M. (2005). 'The retrograde response links metabolism with stress responses, chromatin-dependent gene activation, and genome stability in yeast aging.' Gene 354: 22-27. Jazwinski, S. M. (2005). 'The retrograde response links metabolism with stress responses, chromatin-dependent gene activation, and genome stability in yeast aging.' Gene 354: 22-27. Jeng, W., Lee, S., Sung, N., et al (2015). 'Molecular chaperones: guardians of the proteome in normal and disease states.' F1000Research 4: 1448. Johnson, J. L. (2012). 'Evolution and function of diverse Hsp90 homologs and cochaperone proteins.' Biochim Biophys Acta 1823(3): 607-613. Kaeberlein, M. (2010). 'Lessons on longevity from budding yeast.' Nature 464(7288): 513-519. Kamada, Y., et al. (2010). 'Tor directly controls the Atg1 kinase complex to regulate autophagy.' Mol Cell Biol 30(4): 1049-1058. Kanehisa, M., and Susumu Goto (2000). 'KEGG: Kyoto Encyclopedia of Genes and Genomes.' Nucleic Acids Research 28(1): 27-30. Kenyon, C. J. (2010). 'The genetics of ageing.' Nature 464: 504-512. Lepez-Otin, C., et al. (2013). 'The Hallmarks of Aging.' Cell 153(6): 1194-1217. Lin, S. S., et al. (2003). 'Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing.' J Biol Chem 278(15): 13390-13397. Lin SS, M. J., Gordon JI (2003). 'Sip2, an N-myristoylated beta subunit of Snf1 kinase, regulates aging in Saccharomyces cerevisiae by affecting cellular histone kinase activity, recombination at rDNA loci, and silencing.' J Biol Chem 278(15): 13390-13397. Loewith, R., and Michael N. Hall (2011). 'Target of Rapamycin (TOR) in Nutrient Signaling and Growth Control.' Genetics 189(4): 1177-1201. Longo, V. D., et al. (2012). 'Replicative and chronological aging in Saccharomyces cerevisiae.' Cell Metab 16(1): 18-31. Lu, X. and H. Zhu (2005). 'Tube-gel digestion: a novel proteomic approach for high throughput analysis of membrane proteins.' Mol Cell Proteomics 4(12): 1948-1958. Madia, F., Gattazzo, C., Wei, M., Fabrizio, P., Burhans, W. C., Weinberger, M., … Longo, V. D. (2008). 'Longevity mutation in SCH9 prevents recombination errors and premature genomic instability in a Werner/Bloom model system.' Journal of Cellular Biology 180(1): 67-81. Maiaru, M., et al. (2016). 'The stress regulator FKBP51 drives chronic pain by modulating spinal glucocorticoid signaling.' Sci Transl Med 8(325): 325ra319. Mandal, A., Lee, P., Chen, JA., Nillegoda, N., Heller, A., DiStasio, S,. Oen, H., Victor, J., Nair, DM., Brodsky, JL., Caplan, AJ. (2007). 'Cdc37 has distinct roles in protein kinase quality control that protect nascent chains from degradation and promote posttranslational maturation.' J Cell Biol. 176(3): 319-328. Mattson, M. P. and R. Wan (2005). 'Beneficial effects of intermittent fasting and caloric restriction on the cardiovascular and cerebrovascular systems.' J Nutr Biochem 16(3): 129-137. Mayr, C., Richter, K., Lilie, H., and Johannes Buchner (2000). 'Cpr6 and Cpr7, Two Closely Related Hsp90-associated Immunophilins from Saccharomyces cerevisiae, Differ in Their Functional Properties.' The Journal of Biological Chemistry 275,: 34140-34146. Morano, K. A., and Dennis J.Thiele (1999). 'The Sch9 protein kinase regulates Hsp90 chaperone complex signal transduction activity in vivo.' The EMBO Journal 18(21): 5953–5962. Mortimer, R. K. and J. R. Johnston (1959). 'Life span of individual yeast cells.' Nature 183(4677): 1751-1752. Nathan, D. F., Melissa Harju Vos, and Susan Lindquist (1997). 'In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone.' Proc Natl Acad Sci U S A. 94(24): 12949-12956. Ohlmeier, S., Kastaniotis, A.J., Kalervo Hiltunen, J., and Ulrich Bergmann (2003). 'The Yeast Mitochondrial Proteome, a Study of Fermentative and Respiratory Growth.' Journal of Biological Chemistry 279: 3956-3979. Panaretou, B., Giuliano Siligardi, Philippe Meyer, Alison Maloney, Janis K. Sullivan, Shradha Singh, Stefan H. Millson, Paul A. Clarke, Soren Naaby-Hansen, Rob Stein, Rainer Cramer, Mehdi Mollapour, Paul Workman, Peter W. Piper, Laurence H. Pearl, Chrisostomos Prodromou (2002). 'Activation of the ATPase Activity of Hsp90 by the Stress-Regulated Cochaperone Aha1.' Molecular Cell 10(6): 1307-1318. Pedruzzi, I., et al. (2003). 'TOR and PKA signaling pathways converge on the protein kinase Rim15 to control entry into G0.' Mol Cell 12(6): 1607-1613. Pratt, W., Mario D Galigniana, Jennifer M Harrella, Donald B DeFranco (2004). 'Role of hsp90 and the hsp90-binding immunophilins in signalling protein movement.' Cellular Signalling 16(8): 857-872. Pratt, W., Toft, D. (1999). 'Steroid receptor interactions with heat shock protein and immunophilin chaperones.' Endocr Rev 18(3): 306-360. Puig, O., Caspary, F., Rigaut, G., Rutz ,B., Bouveret, E., Bragado-Nilsson, E., Wilm, M., Séraphin, B., (2001). 'The Tandem Affinity Purification (TAP) Method: A General Procedure of Protein Complex Purification.' Method 24(3): 218-229. Rabindran, S. K., Wisniewski, J., Li, L., Li, G.C., Wu, C. (1995). 'Interaction between heat shock factor and hsp70 is insufficient to suppress induction of DNA-binding activity in vivo.' Mol Cell Biol. 14(10): 6552-6560. Rey, A., L. SIA AND AARON P. MITCHELL (1995). 'Stimulation of Later Functions of the Yeast Meiotic Protein Kinase Ime2p by the IDS2 Gen.' MOLECULAR AND CELLULAR BIOLOGY 15(10): 5279–5287. Riggs, D. L., Marc B. Co, Joyce Cheung-Flynn, Viravan Prapapanich, Patricia E. Carrigan & David F. Smith (2004). 'Functional Specificity of Co-Chaperone Interactions with Hsp90 Client Proteins.' Critical Reviews in Biochemistry and Molecular Biology 39(5-6). Roskoski, R. (1983). 'Assays of protein kinase.' Methods in Enzymology 99: 3-6. Roth, G. S., et al. (2001). 'Caloric restriction in primates and relevance to humans.' Ann N Y Acad Sci 928: 305-315. Sakurai, H. and A. Ota (2011). 'Regulation of chaperone gene expression by heat shock transcription factor in Saccharomyces cerevisiae: importance in normal cell growth, stress resistance, and longevity.' FEBS Lett 585(17): 2744-2748. Storer, C. L., et al. (2011). 'FKBP51 and FKBP52 in signaling and disease.' Trends in Endocrinology and Metabolism 22(12): 481-490. Toone, W. M. and N. Jones (1998). 'Stress-activated signalling pathways in yeast.' Genes Cells 3(8): 485-498. Tsai, C. F., et al. (2008). 'Immobilized metal affinity chromatography revisited: pH/acid control toward high selectivity in phosphoproteomics.' J Proteome Res 7(9): 4058-4069. Tsou, C. C., et al. (2010). 'IDEAL-Q, an automated tool for label-free quantitation analysis using an efficient peptide alignment approach and spectral data validation.' Mol Cell Proteomics 9(1): 131-144. van Oosten-Hawle, P., Morimoto, R. (2014). 'Organismal proteostasis: role of cell-nonautonomous regulation and transcellular chaperone signaling.' Genes & Development 28(14): 1533-1543. Verghese, J., Abrams, J., Wang, Y., Morano, K.A. (2012). 'Biology of the heat shock response and protein chaperones: budding yeast (Saccharomyces cerevisiae) as a model system.' Microbiol Mol Biol Rev 76(2): 115–158. Voellmy, R., Boellmann, F. (2007). 'Chaperone regulation of the heat shock protein response.' Adv Exp Med Biol. 594: 89-99. Wadskog, I., Maldener, C., Proksch, A., Madeo, F., and Lennart Adler (2004). 'Yeast Lacking the SRO7/SOP1-encoded Tumor Suppressor Homologue Show Increased Susceptibility to Apoptosis-like Cell Death on Exposure to NaCl Stress.' Mol Biol Cell 15(3): 1436-1444. Wang, P. a. J. H. (2005). 'The cyclophilins.' Genome Biol 6(7): 226. Wang, Y. T., et al. (2010). 'An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion.' J Proteome Res 9(11): 5582-5597. Wanke, V., Cameroni, E., Uotila, A., Piccolis, M., Urban, J., Loewith, R. and De Virgilio, C. (2008). 'Caffeine extends yeast lifespan by targeting TORC1.' Molecular Microbiology 69: 277–285. Wei, M., Fabrizio, P., Hu, J., Ge, H., Cheng, C., Li, L., Longo VD. (2008). 'Life span extension by calorie restriction depends on Rim15 and transcription factors downstream of Ras/PKA, Tor, and Sch9.' PLoS Genetic Volume 4 (1): 139-149. Werner-Washburne, M., Brown, D., Braun, E.. (1991). 'Bcy1, the regulatory subunit of cAMP-dependent protein kinase in yeast, is differentially modified in response to the physiological status of the cell.' J Biol Chem 266(29): 19704-19709. Whitesell, L., Mimnaugh Edward G., De Costa Brian, Myers Charles E., and Neckers Leonard M. (1994). 'Inhibition of Heat Shock Protein HSP90-pp60v-src Heteroprotein Complex Formation by Benzoquinone Ansamycins: Essential Role for Stress Proteins in Oncogenic Transformation.' Proceedings of the National Academy of Sciences of the United States of America 91(18): 8324-8328. Winzeler, E. A., et al. (1999). 'Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.' Science 285(5429): 901-906. Yong, W., Yang, Z., Periyasamy, S., Chen, H., Yucel, S., Li, W., … Shou, W. (2008). 'Essential Role for Co-Chaperone FKBP52 but not FKBP51 in Androgen Receptor-Mediated Signaling and Physiology.' The Journal of biological chemistry. 282(7): 5026-5036. Zaman, S., et al. (2008). 'How Saccharomyces responds to nutrients.' Annu Rev Genet 42: 27-81. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/49702 | - |
dc.description.abstract | 老化是種具有生理功能的逐漸喪失相關聯的複雜的現象。其本身是由內在因素和外在因素所調節。以前的研究表明,熱量限制延長壽命主要是透許多磷酸化激酶的參與下,以磷酸化蛋白間的訊息傳遞所調控的.;然而,這些信號的磷酸化途徑是如何協調的詳細分子機制仍然知之甚少。在這裡,我們實驗室利用質譜儀的技術大規模地去尋找在限制熱量攝入的酵母磷酸化和去磷酸化位點。質譜總共定量出1244磷酸化/去磷酸化位點,共632磷酸化蛋白有受到調控的變化。 從發現的磷酸化蛋白選出110個進行功能篩選,最後篩篩到一個受到PKA磷酸化的有絲分裂相關的未知功能蛋白IDS2,在老化進程作出貢獻的一個關鍵調控。 IDS2會扮演著一個輔助性熱休克蛋白,與熱休克蛋白Hsc82和冗餘的Hsp82形成複合物。 IDS2剔除基因或IDS2模擬磷酸化菌株皆會產生熱敏性和壽命縮短的現象。而需要HSP90輔佐其折疊的蛋白會在IDS2剔除或IDS2模擬磷酸化的細胞不穩定。IDS2去磷酸化則是會恢復這些表型。從這些結果皆可得知,酵母菌偵測熱量攝入的變化,進而透過PKA的活化與否去影響到HSP90伴侶相關的折疊的活性,並由此影響個體的壽命。 | zh_TW |
dc.description.abstract | Aging, an intricate phenomenon associated with the gradual loss of physiological functions, is regulated by intrinsic and extrinsic factors. Previous studies indicate that calorie restriction extends the life span of a variety of species due to the involvement of multiple kinase regulations; however, the molecular mechanism of how these signaling phosphorylation pathways are coordinated is still poorly understood. Here we globally identify yeast phosphorylation and dephosphorylation sites under calorie restriction. Quantitative mass spectrometry identified a total of 1244 phosphorylation/ dephosphorylation sites on 632 proteins. Functional screen of the 110 potential regulators discovered a mitotically functional unknown protein Ids2 phosphorylated by PKA under rich medium, contributing to a key regulation in aging process. Ids2 serves as a cochaperone to form a complex with Hsc82 and the redundant Hsp82. ids2 or ids2 phosphor-mimicking cells displayed heat sensitivity and life span shortening. The in-vivo substrate of HSP90 is destabilized in ids2 or ids2 phosphor-mimicking cells. Dephosphorylation of this Ids2 phosphorylation recovered these phenotypes. Taken together, yeast cells sense calorie intake and compromise HSP90 chaperone folding activity through PKA, and thereby influence the lifespan. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T11:42:55Z (GMT). No. of bitstreams: 1 ntu-105-R03445131-1.pdf: 4975955 bytes, checksum: 1ad153136384d75c8aeee4103e37285f (MD5) Previous issue date: 2016 | en |
dc.description.tableofcontents | CONTENTS
摘要. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i ABSTRACT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ii 謝誌. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii TABLE OF CONTENTS . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iv INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1 MATERIALS & METHODS Yeast Strains and Plasmids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 Tandem Affinity Purification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 Samples Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 LC-MS/MS Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . .11 Bioinformatics Analysis - Gene Ontology Enrichment Analysis & Kegg’s Othology. . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13 Stress Resistance Assay. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Yeast Two Hybrid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14 Gel electrophoresis and Western blot analysis . . . . . . . . . . . . . . . . . . . . . . . . . .15 Choronlogical Life Span. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 In-vitro Kinase Assay. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16 In-vitro Pull Down Assay. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 RESULTS Identification of (de)Phosphorylated Proteins and Their Sites under Calorie Restriction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 Specification of Mass Spectrometry-derived Candidates with Bioinformatics, Databases and Functional Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 PKA Regulates Ids2 S148 under CR condition. . . . . . . . . . . . . . . . . . . . . . . . . . . 22 Interaction Between Ids2 and the Yeast Chaperone Protein HSP90 Regulates Heat Shock and Life Span in Yeast.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24 Ids2 may participate in the coordination of Hsp90-regulated chaperone protein folding response. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 DISCUSSION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 FIGURES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Fig.1: Integrated experimental and computational pipeline to determine in vivo phosphorylation or dephosphorylation substrates under CR condition. Fig.2: Schematic Representation of Mass Spectrometry-derived Candidates Specified with Bioinformatic Databases Fig.3: PKA regulates Ids2 S148 under CR condition Fig.4: Interaction between Ids2 and the yeast chaperone protein HSP90 regulates heat shock and life span in yeast. Fig.5: Ids2 may participate in the coordination of Hsp90-regulated chaperone protein folding response. TABLES. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 Table.1 Yeast Strains and Plasmids Used in This Study Table.2 Oligonucleotides Used in this Study REFERENCES. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54 | |
dc.language.iso | en | |
dc.title | 探討熱量攝取和PKA所調控的熱休克輔助性蛋白磷酸化與細胞壽命的關聯性 | zh_TW |
dc.title | Calorie-driven PKA-mediated
Co-chaperone Phosphorylation Controls Life Span | en |
dc.type | Thesis | |
dc.date.schoolyear | 104-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林敬哲(Jing-Jer Lin),李財坤(Tsai-Kun Li) | |
dc.subject.keyword | 老化,熱量限制,磷酸化,PKA,Hsp90, | zh_TW |
dc.subject.keyword | aging,calorie restriction,phosphorylation,PKA,HSP90, | en |
dc.relation.page | 62 | |
dc.identifier.doi | 10.6342/NTU201602696 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2016-08-15 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-105-1.pdf 目前未授權公開取用 | 4.86 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。