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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 盧國賢 | |
dc.contributor.author | Shu-Ning Chang | en |
dc.contributor.author | 張書寧 | zh_TW |
dc.date.accessioned | 2021-06-15T05:25:17Z | - |
dc.date.available | 2010-09-09 | |
dc.date.copyright | 2010-09-09 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-07-16 | |
dc.identifier.citation | 七、參考文獻 (References)
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Br J Cancer 84, 1488-1496. Steeg, P.S., and Theodorescu, D. (2008). Metastasis: a therapeutic target for cancer. Nat Clin Pract Oncol 5, 206-219. Sternlicht, M.D., and Werb, Z. (2001). How matrix metalloproteinases regulate cell behavior. Annu Rev Cell Dev Biol 17, 463-516. Sun, B., Hoshino, J., Jermihov, K., Marler, L., Pezzuto, J.M., Mesecar, A.D., and Cushman, M. (2010). Design, synthesis, and biological evaluation of resveratrol analogues as aromatase and quinone reductase 2 inhibitors for chemoprevention of cancer. Bioorg Med Chem. Surh, Y. (1999). Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances. Mutat Res 428, 305-327. Swiatecka, J., Dzieciol, J., Anchim, T., Dabrowska, M., Pietruczuk, M., and Wolczynski, S. (2000). Influence of estrogen, antiestrogen and UV-light on the balance between proliferation and apoptosis in MCF-7 breast adenocarcinoma cells culture. Neoplasma 47, 15-24. Tang, F.Y., Chiang, E.P., and Sun, Y.C. (2008). Resveratrol inhibits heregulin-beta1-mediated matrix metalloproteinase-9 expression and cell invasion in human breast cancer cells. J Nutr Biochem 19, 287-294. Tang, X., Zhang, Q., Nishitani, J., Brown, J., Shi, S., and Le, A.D. (2007). Overexpression of human papillomavirus type 16 oncoproteins enhances hypoxia-inducible factor 1 alpha protein accumulation and vascular endothelial growth factor expression in human cervical carcinoma cells. Clin Cancer Res 13, 2568-2576. Thiery, J.P. (2002). Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer 2, 442-454. Valenzano, D.R., Terzibasi, E., Genade, T., Cattaneo, A., Domenici, L., and Cellerino, A. (2006). Resveratrol prolongs lifespan and retards the onset of age-related markers in a short-lived vertebrate. Curr Biol 16, 296-300. Wallace, D.C. (2005). A mitochondrial paradigm of metabolic and degenerative diseases, aging, and cancer: a dawn for evolutionary medicine. Annu Rev Genet 39, 359-407. Wang, Q., Xu, J., Rottinghaus, G.E., Simonyi, A., Lubahn, D., Sun, G.Y., and Sun, A.Y. (2002). Resveratrol protects against global cerebral ischemic injury in gerbils. Brain Res 958, 439-447. Wijnhoven, B.P., Dinjens, W.N., and Pignatelli, M. (2000). E-cadherin-catenin cell-cell adhesion complex and human cancer. Br J Surg 87, 992-1005. Wolter, F., and Stein, J. (2002). Resveratrol enhances the differentiation induced by butyrate in caco-2 colon cancer cells. J Nutr 132, 2082-2086. Woo, J.H., Lim, J.H., Kim, Y.H., Suh, S.I., Min, D.S., Chang, J.S., Lee, Y.H., Park, J.W., and Kwon, T.K. (2004). Resveratrol inhibits phorbol myristate acetate-induced matrix metalloproteinase-9 expression by inhibiting JNK and PKC delta signal transduction. Oncogene 23, 1845-1853. Yang, J., and Weinberg, R.A. (2008). Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev Cell 14, 818-829. Yoshiji, H., Harris, S.R., and Thorgeirsson, U.P. (1997). Vascular endothelial growth factor is essential for initial but not continued in vivo growth of human breast carcinoma cells. Cancer Res 57, 3924-3928. Zhang, Q., Tang, X., Lu, Q.Y., Zhang, Z.F., Brown, J., and Le, A.D. (2005). Resveratrol inhibits hypoxia-induced accumulation of hypoxia-inducible factor-1alpha and VEGF expression in human tongue squamous cell carcinoma and hepatoma cells. Mol Cancer Ther 4, 1465-1474. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/46718 | - |
dc.description.abstract | 中文摘要
Resveratrol (白藜蘆醇, 3,4,5'-trihydroxy-trans-stilbene)是一種存在植物內天然的多酚類 (polyphenol)化合物,本實驗採用乳腺癌細胞株 (MDA-MB-231cell line)進行細胞培養的研究,探討resveratrol對MDA-MB-231 cells生長、細胞移行與侵襲的影響,實驗結果如下(1) MTT assay實驗結果顯示: 細胞經resveratrol處理24小時後,高濃度的resveratrol (30, 50, 70μΜ)與未給予藥物之控制組相較,會促使MDA-MB-231細胞存活率降低 (83%, 74%, 22%),達到顯著差異,且細胞株處理不同時間 (24, 48, 72小時),細胞生長比例皆低於控制組,均達到顯著差異。西方墨點分析顯示: resveratrol可透過減少抗細胞凋亡bcl2蛋白表現及增加促細胞凋亡caspase3蛋白表現,而造成細胞凋亡;(2) 細胞移行分析與細胞傷口癒合分析結果顯示: 不同濃度的resveratrol (30, 50μΜ)處理下,細胞的移行能力降低,統計上達到顯著的差異。免疫細胞螢光染色結果顯示: resveratrol (30, 50μΜ)可以正向調控 MDA-MB 231細胞株E-cadherin的表現,以及抑制細胞內皮生長因子(VEGF)的表現。(3) 細胞侵襲分析結果顯示: 不同濃度的resveratrol (30、50μΜ)處理下,細胞侵襲的細胞數下降,統計上達到顯著的差異。西方墨點分析結果顯示: 不同濃度的resveratrol ( 30, 50μΜ)處理下,使基質金屬蛋白酶9 (MMP9)的表現下降。另外,利用明膠蛋白酵素電泳法證實經resveratrol ( 30, 50μΜ)處理下,細胞分泌至細胞外的MMP9之活性降低。 綜合以上結果顯示: resveratrol抑制乳腺癌MDA-MB-231 cells生長、移行與侵襲的過程可能透過抑制bcl2、VEGF與MMP9蛋白的表現及活化caspase3與E-cadherin的表現,進而達到抑制MDA-MB-231 cells生長、移行與侵襲的目的。 | zh_TW |
dc.description.abstract | 英文摘要
Resveratrol is a natural polyphenol compound within the plant. We used MDA-MB-231 breast adenocarcinoma cell line as a model to investigate the effects of resveratrol on proliferation, migration, and invasion of breast cancer cell. By MTT assay, we found that after treatment with 30, 50, 70μΜ resveratrol for 24 hours, the proliferation ratio of MDA-MB-231 cells decreased to 83%, 74%, 22%. The results of time course study also (24, 48, 72hours) revealed that the proliferation ratio of 30, 50, 70μΜ resveratrol-treated MDA-MB-231 cells were decreased. Western blotting showed that resveratrol caused cell apoptosis resulting from the suppression of bcl2 protein expression and activation of caspase3 protein expression. In cell migration assay and wound healing assay revealed that MDA-MB-231 cells treated with resveratrol decrease the migration of cells. The effects of resveratrol on the expression of E-cadherin and VEGF by MDA-MB-231 were analyzed by fluorescence immunocytochemistry stain. Results indicate that resveratrol up-regulated E-cadherin expression and down-regulated VEGF expression. By cell invasion assay, we found that after being treated with resveratrol, the cells decreased in invasion ability. Western blotting showed that resveratrol caused the suppression of MMP9 expression. Besides, the activation of MMP9 secreted to the extracellular fluid, analyzed by gelatin zymography, was decreased in MDA-MB-231 cell line after being treated with resveratrol. The results obtained from the present study showed that resveratrol might play an important role in the suppression of the proliferation, migration and invasion of breast cancer cell line of MDA-MB-231 cells through the inhibition of bcl2, VEGF and MMP9 and activation of caspase3 and E-cadherin. | en |
dc.description.provenance | Made available in DSpace on 2021-06-15T05:25:17Z (GMT). No. of bitstreams: 1 ntu-99-R96446003-1.pdf: 3808491 bytes, checksum: 985f87e33b637d5eb06bdac79eafdc24 (MD5) Previous issue date: 2010 | en |
dc.description.tableofcontents | 目錄
中文摘要 3 英文摘要 4 縮寫表 6 一、 緒言(Introduction) 7 1.1白藜蘆醇 (Resveratrol) 7 1.2乳癌 9 二、 目的(Purposes) 11 三、 材料與方法(Material and Methods) 3.1試劑(Reagents) 12 3.2抗體(Antibodies) 13 3.3細胞培養(Cell culture) 13 3.4細胞存活率測定(MTT assay) 13 3.5西方墨點轉漬法 (Western blotting) 14 3.6螢光免疫細胞化學染色(Fluorescence immunocytochemistry stain) 15 3.7細胞傷口癒合分析(Wound healing assay) 16 3.8細胞移行分析(Cell migration assay) 16 3.9細胞侵襲分析(Cell invasion assay) 16 3.10明膠蛋白酵素電泳法(Gelatin zymography) 17 3.11數據統計分析 18 四、 結果(Results) 4.1高濃度的resveratrol降低乳腺癌細胞增生 19 4.2 Resveratrol於長時間更顯著抑制乳腺癌細胞生長 19 4.3 Resveratrol改變乳腺癌細胞的bcl2及caspase3的表現 20 4.4 Resveratrol影響乳腺癌細胞的形態與細胞間的連結分子 20 4.5 Resveratrol使乳腺癌細胞中內皮血管生長因子(VEGF)表 現減少 21 4.6 Resveratrol使乳腺癌細胞移行能力下降 21 4.7 Resveratrol使乳癌細胞侵襲能力下降 22 五、 討論(Discussions) 5.1Resveratrol的化學結構 24 5.2Resveratrol抑制乳腺癌細胞MDA-MB-231的生長 25 5.3 Resveratrol抑制乳腺癌細胞MDA-MB-231的移行 26 5.4 Resveratrol抑制乳腺癌細胞MDA-MB-231的侵襲 28 5.5 Resveratrol的相關研究與機轉 29 六、 結論(Conclusions) 與未來擬繼續進行之研究工作(Further works) 30 七、 參考文獻(References) 31 八、 圖片說明(Figures and Figure Legends) 36 | |
dc.language.iso | zh-TW | |
dc.title | Resveratrol抑制人類乳腺癌細胞株MDA-MB-231的生長、移行和侵襲能力 | zh_TW |
dc.title | Resveratrol suppresses the proliferation, migration and invasion of human breast cancer cell line MDA-MB-231 | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 錢宗良,周逸鵬 | |
dc.subject.keyword | 白藜蘆醇,乳癌, | zh_TW |
dc.subject.keyword | Resveratrol,breast cancer, | en |
dc.relation.page | 58 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2010-07-16 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 解剖學暨生物細胞學研究所 | zh_TW |
顯示於系所單位: | 解剖學暨細胞生物學科所 |
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