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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王一雄 | |
dc.contributor.author | Bing-Sheng Chen | en |
dc.contributor.author | 陳炳勝 | zh_TW |
dc.date.accessioned | 2021-06-13T06:45:07Z | - |
dc.date.available | 2010-08-01 | |
dc.date.copyright | 2005-08-01 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-29 | |
dc.identifier.citation | 邱建中。1985。醯胺(Amide)類殺草劑。台中區農推資訊第四十五期。pp.1-8.
邱建中,鍾維榮。1996。殺草劑與雜草防除。pp.1-11。中華民國雜草協會和台中區農業改良場。台北。 袁紹英,張碧芬。1999。土壤受多環芳香族碳氫化合物污染之生物整治。第六屆土壤污染防治協會論文集。pp.19-35. 張秀齡,2001。醯胺類除草劑在不同土壤中的吸附、降解及移動之研究。國立台灣大學農業化學研究所碩士論文。. 劉建昌,2003。茶園土壤中具降解醯胺類農藥除草劑能力之菌株篩選與其特性分析。國立台灣大學農業化學研究所。. 潘銓泰,2001。生物復育技術處理砂岸油污之研究。國立中山大學海洋環境及工程學系碩士論文。 蔡竹固,1996。殺草劑之化學分類。嘉義農專農藝學報第二十八期。pp.21-43. Amann, R. I., W. Ludwig, and K.-H. Schleifer 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev 59(1): 143-169. Bartha, R., R. P. Lanzillota, and D. Pramer. 1967. Stability and effects of some pesticides in soil. Appl. Microbiol 15: 67-75. Bedient, P. B., Rifai, H. S., and Newell, C. J. 1999. Ground Water Contamination – Transport and Remediation. PTR Prentice-Hall Prentice-Hall Inc. 604. Bollen, W. B. 1961. Interactions between pesticides and soil microorganisms. Ann. Rev. Microbial 15: 69-92. Chandra, P., and W. B. Bollen. 1961. Effects of nabam and mylone on nitrification, soil respiration and microbial numbers in four origan soils. Soil Sci 92: 387-393. Chesters, G., G. V. Simsiman, J. Levy, B. J. Alhajjar, R. N. Fathulla, and J. M. Harkin. 1989. Environmental fate of alachlor and metolachlor. Rev. Environ. Contam. Toxicol. 110: 1-74. Cowan, D. A. 2000. Microbial genomes -- the untapped resource. Trends in biotech. 18:14-16. Galassi, S., A. Provini, S. Mangiapan, and E. Benfenati. 1996. Alachlor and its metabolites in surface water. Chemosphere.32(2): 229-237. Helling, C. S., P. C. Kearney, and M. Alexander. 1971. Behavior of pesticides in soils. Advan. Agron. 23: 147-240. Kao, C. M., and Prosser, N. R. 1999. Influence of aquifer bioecochemical distribution on BTEX biodegradation under aerobic/anaerobic conditions. J. of Chinese Institute of Environment Engineering 9: 187-197. Kreutzer, W. A. 1963. Selective toxicity of chemicals to soil microorganisms. Ann. Rev. Phytopath 1: 101-126. Ranjard, L., F. Poly, and S. Nazaret 2000. Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment. Res. Microbiol 151: 167-177. Ranjard, L., F. Poly., and S. Nazaret. 2000. Monitoring complex bacterial communities using culture-independent molecular techniques: application to soil environment. Res. Microbiol. 151:167-177. Schlöter, M., M. Lebuhn, T. Heulin, and A. Hartmann. 2000. Ecology and evolution of bacterial microdiversity FEMS. Microbiol. Rev. 24:647-660 Tomlin, C. D. S. 1997a. pp. 23-24. The Pesticide Manual, 11th ed. British Crop Protection Council, UK. Tomlin, C. D. S. 1997b. pp. 833-834. The Pesticide Manual, 11th ed. British Crop Protection Council, UK. Tomlin, C. D. S. 1997c. pp. 1013-1015. The Pesticide Manual, 11th ed. British Crop Protection Council, UK. Tu, C. M. 1970. Effect of four organophosphorus insecticides on microbial activities in soil. Appl. Microbiol 19: 479-484. Walker, A., and S. J. Welch. 1991. Enhanced degradation of some soil-applied herbicides. Weed Res. 31(1): 49-57. Walker, A., Y. H. Moon, and S. J. Welch. 1992. Influence of temperature, soil moisture and soil characteristics on the persistence of alachlor. Pestic. Sci. 35: 109-116. Ward, D. M., R. Weller, and M. M. Bateson. 1990. 16S rDNA sequences reveal numerous uncultured microorganisms in a natural community. Nature 62: 1353-1370. Woese, C. R. 1987. Bacterial evolution. Microbiol. Rev. 51: 221-271. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/35239 | - |
dc.description.abstract | 本篇研究目的,為施用具降解醯胺類除草劑能力之菌株於土壤樣品中,並探討這些菌株在不同土壤中的適應情形及其對兩種除草劑拉草 (Alachlor)、滅多草 (Metolachlor)的降解能力。本實驗使用之土壤,分別採集自農業委員會桃園農業改良場及苗栗縣大湖鄉觀光草莓園。實驗設計為三種不同水分含量 (原始、60 % W.H.C、90 % W.H.C),之後分別接種菌株MA3 (Bacillus thuringiensis)與菌株RM2 (Bacillus cereus)於實驗土壤中並加入待測除草劑。實驗所選之兩種除草劑,依據其田間施用量分別調整濃度為10 與50 μg ml-1。最後於不同天數中,分別分析土壤中之除草劑降解及菌株適應情形。實驗結果顯示,可藉由目標菌株之PCR產物所製成的marker定性出目標菌株於DGGE指紋譜上的位置,並以此觀測出目標菌株於土壤中適應情形,結果適應良好。在除草劑降解方面,吾人欲瞭解額外添加菌株是否會影響降解速率,土壤中水分含量多寡並不會影響醯胺類農藥的消散情形。添加具降解醯胺類農藥能力之菌株進入土壤添加具降解醯胺類除草劑能力菌株除了在二仁系土壤、10 μg mL-1滅多草的處理中,可以使降解速率約提高10 %外,其餘的處理皆無法顯著的提高降解速率。以PCR-DGGE觀測施用醯胺類除草劑對於土壤微生物族群的影響,發現施用除草劑會對土壤微生物造成衝擊,而水分多寡會影響醯胺類除草劑對土壤微生物菌相的影響。添加具降解醯胺類除草劑能力菌株於土壤當中,並不能減低醯胺類農藥對土壤微生物菌相的影響。較高濃度的醯胺類除草劑對土壤中微生物菌相的衝擊也越大。 | zh_TW |
dc.description.abstract | The objective of this study is to confer the the adaptability of acetanilide herbicides degrading bacteria in fields and to study the the adaptability of acetanilide herbicides degrading bacteria in fields and the ability of the bacteria to degrade amide herbicide.We applied the bacteria into soil and analysised the adaptability of the bacteria and the difference between microbial community with PCR-DGGE. HPLC was used to detect the degradation of the amide herbicide.According to the result,the bacteria was well-adapted and could increase the degradation rate at 10 ug g-1 metolachlor in the Er-Jen soil.The soil microbial community were affected by the amide herbicide.The higher concentration of the herbicide, the greater influence from the herbicide to soil microbial community. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T06:45:07Z (GMT). No. of bitstreams: 1 ntu-94-R92623008-1.pdf: 1544199 bytes, checksum: 407fbb8302251b48ddb1a82fad2aee28 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 中文摘要……………………………………………………………. I
英文摘要……………………………………………………………. II 目錄…………………………………………………………………. III 表目錄………………………………………………………………. IV 圖目錄………………………………………………………………. V 第一章 前言………………………………………………………... 1 第二章 材料與方法…………………………………………........... 10 第三章 結果與討論…………………………………………........... 31 第四章 結論…………………………………………………........... 64 第五章 參考文獻……………………………………………........... 65 | |
dc.language.iso | zh-TW | |
dc.title | 施用具降解醯胺類除草劑能力菌株於土壤適應性之研究 | zh_TW |
dc.title | The Adaptability of Acetanilide Herbicides Degrading Bacteria in Soils | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 顏瑞泓 | |
dc.contributor.oralexamcommittee | 李國欽,劉秀美,江漢全,袁紹英 | |
dc.subject.keyword | 降解,拉草,滅多草,變性梯度凝膠電泳, | zh_TW |
dc.subject.keyword | Degradation,Alachlor,Metolachlor,Denaturing gradient gel electrophoresis(DGGE), | en |
dc.relation.page | 68 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2005-07-29 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
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