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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 龐飛(Victor Fei Pang) | |
dc.contributor.author | Yi-Ting Chen | en |
dc.contributor.author | 陳怡廷 | zh_TW |
dc.date.accessioned | 2021-06-13T05:44:27Z | - |
dc.date.available | 2011-08-03 | |
dc.date.copyright | 2011-08-03 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-07-26 | |
dc.identifier.citation | 行政院農業委員會動植物防疫檢疫局,2007,冷凍豬精液輸入檢疫條件,2010/05/13 downloaded. http://www.baphiq.gov.tw/public/Attachment/6102715543871.pdf
夏良宙,2008,使用稀釋精液應注意事項,現代養豬雜誌,10,63-73。 陳中興,2009,養豬場諮詢服務體系推動情形與執行成果, 農牧旬刊,1619,45-48。 陳銘正,2004,種豬精液之品質監控與分級供應。豬育種策略研討會。 劉世華、詹木本、郭有海,2004,人工授精站在豬隻供精體系的角色。豬育種策略研討會。 劉振軒、張淑美、蔡明翰、李細祥,2009,弓形蟲感染症,人畜共通傳染病臨床指引(第二版),343-347 蕭君倪,2010,建立偵測常見豬隻呼吸道病毒性豬隻病毒性疾病即時聚合酶鍊鎖反應技術,國立台灣大學獸醫學系碩士。 Allan, G.M., Ellis, J.A., 2000, Porcine circoviruses: a review. J Vet Diagn Invest 12, 3-14. Allan, G.M., Kennedy, S., McNeilly, F., Foster, J.C., Ellis, J.A., Krakowka, S.J., Meehan, B.M., Adair, B.M., 1999, Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121, 1-11. AQIS (2000). Animal Quarantine Policy Memorandum. 2010/05/06 downloaded. http://www.daff.gov.au/_data/assets/pdf_file/0016/15343/00-020.pdf Bachmann, P.A., Hoggan, M.D., Kurstak, E., Melnick, J.I., Pereira, H.G., Tattersall, P., Vago, C., 1979, Parvoviridae: Second report. Intervirology 11, 248-254. Becher, P., Orlich, M., Kosmidou, A., Konig, M., Baroth, M., Thiel, H.J., 1999, Genetic diversity of pestiviruses: Identification of novel groups and implications for classification. Virology 262, 64-71. Benfield, D.A., Nelson, C., Steffen, M., Rowland, R.R., 2000, Transmission of PRRSV by artificial insemination using extended semen seeded with different concentrations of PRRSV. Proc Annu Meet Am Assoc Swine Pract, pp. 405-408. Bloemraad, M., de Kluijver, E.P., Petersen, A., Burkhardt, G.E., Wensvoort, G., 1994, Porcine reproductive and respiratory syndrome: temperature and pH stability of Lelystad virus and its survival in tissue specimens from viraemic pigs. Vet Microbiol 42, 361-371. Bolin, C.A., Cassells, J.A., 1992, Isolation of Leptospira interrogans serovars bratislava and hardjo from swine at slaughter. J Vet Diag Invest 4, 87-89. Buescher, E.L., Scherer, W.F., 1959, Ecological studies of Japanese encephalitis virus in Japan. IX. Epidemiologic correlations and conclusions. Am J Trop Med Hyg 8, 719-722. Burke, D.S., Monath, T.P., 2001, Flaviviruses. In: Kinipe DM, Howley PM (ed). Fields Virology, Lippincott-Raven Philadelphia, pp. 991-1024. Cartwright, S.F., Huck, R.A., 1967, Viruses isolated in association with herd infertility, abortions and stillbirths in pigs. Vet Rec 81, 196-197. Cartwright, S.F., Lucas, M., Huck, R.A., 1969, A small haemagglutinating porcine DNA virus. I. Isolation and properties. J Comp Pathol 79, 371-377. Chen, H.Y., Li, X.K., Cui, B.A., Wei, Z.Y., Li, X.S., Wang, Y.B., Zhao, L., Wang, Z.Y., 2009, A TaqMan-based real-time polymerase chain reaction for the detection of porcine parvovirus. J Virol Methods 156, 84-88. Cheng, D., Zhao, J.J., Li, N., Sun, Y., Zhou, Y.J., Zhu, Y., Tian, Z.J., Tu, C., Tong, G.Z., Qiu, H.J., 2008, Simultaneous detection of Classical swine fever virus and North American genotype Porcine reproductive and respiratory syndrome virus using a duplex real-time RT-PCR. J Virol Methods 151, 194-199. Choi, C., Chae, C., 2000, Distribution of porcine parvovirus in porcine circovirus 2-infected pigs with postweaning multisystemic wasting syndrome as shown by in-situ hybridization. J Comp Pathol 123, 302-305. Choi, C., Chae, C., 2003, Detection of classical swine fever virus in boar semen by reverse transcription-polymerase chain reaction. J Vet Diagn Invest 15, 35-41. Christianson, W.T., Choi, C.S., Collins, J.E., Molitor, T.W., Morrison, R.B., Joo, H.S., 1993, Pathogenesis of porcine reproductive and respiratory syndrome virus infection in mid-gestation sows and fetuses. Can Vet Res 57, 262-268. Christopher-Hennings, J., 1995, Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR. J Clin Microbiol 33, 1730-1734. Christopher-Hennings, J., Nelson, E., Hines, R., Nelson, J., Swenson, S., Zimmerman, J., 1995, Persistence of porcine reproductive and respiratory syndrome virus in serum and semen of adult boars. J Vet Diagn Invest 7, 456-464. Christopher-Hennings, J., Nelson, E.A., Benfield, D.A., 1996, Detecting PRRSV in boar semen. J Swine Health Prod 4, 37-39. Christopher-Hennings, J., Nelson, E.A., Nelson, J.K., Benfield, D.A., 1997, Effects of a modified-live vaccine against porcine reproductive and respiratory syndrome in boars. Am J Vet Res 58, 40-45. Ciacci-Zanella, J.R., Zanella, E.L., Locatelli, M.L., Brambatti, J.L., Simon, N.L., Coldebella, M., 2007, Detection of porcine circovirus 2 in semen collected from naturally infected boars study in Brazil. In: Proceedings of 5th International Symposium on Emerging and Re-emerging Pig Disease, pp. 94. Collins, J.E., Benfield, D.A., Christianson, W.T., Harris, L., Hennings, J.C., Shaw, D.P., Goyal, S.M., McCullough, S., Morrison, R.B., Joo, H.S., Gorcyca, D., Chladek, D., 1992, Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of disease in gnotobiotic pigs. J Vet Diagn Invest 4, 117-126. Daniels, P.W., Williams, D.T., Mackenzie, J.S., 2002, Japanese encephalitis virus. In: Morilla, A., Yoon, K.J., Zimmerman, J.J., Trends in Emerging Viral Infections of Swine, Iowa State Press, Ames, pp. 249-263. De Smit, A.J., Bouma, A., Terpstra, C., van Oirschot, J.T., 1999, Transmission of classical swine fever virus by artificial insemination. Vet Microbiol 67, 239-249. Dee, S., Deen, J., Rossow, K., Weise, C., Eliason, R., Otake, S., Joo, H.S., Pijoan, C., 2003, Mechanical transmission of porcine reproductive and respiratory syndrome virus throughout a coordinated sequence of events during warm weather. Can J Vet Res 67, 12-19. Dee, S.A., Deen, J., Rossow, K., Wiese, C., Otake, S., Joo, H.S., Pijoan, 2002, Mechanical transmission of porcine and respiratory syndrome virus throughout a coordinated sequence of events during cold weather. Can J Vet Res 66, 232-239. Elbers, A.R.W., Stegeman, A., Moser, H., Ekker, H.M., Smak, J.A., Pluimers, F.H., 1999, The classical swine fever epidemic 1997-1998 in the Netherlands: Descriptive epidemiology. Prev Vet Med 42, 157-184. Ellis, J.A., Bratanich, A., Clark, E.G., Allan, G., Meehan, B., Haines, D.M., Harding, J., West, K.H., Krakowka, S., Konoby, C., Hassard, L., Martin, K., McNeilly, F., 2000, Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12, 21-27. Ellis, J.S., Smith, J.W., Braham, S., Lock, M., Barlow, K., Zambon, M.C., 2007, Designand validation of an H5 TaqMan real-time one-step reverse transcription-PCR and confirmatory assays for diagnosis and verification of influenza A virus H5 infections in humans. J Clin Microbiol 45, 1535-1543. Ellis, W.A., 2006, Leptospirosis. In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 691-700. Ellis, W.A., McParland, P.J., Bryson, D.G., Thiermann, A.B., Montgomery, J., 1986, Isolation of leptospires from genital tract and kidneys of aborted sows. Vet Rec 118, 294-295. Feitsma, H., Grooten, H.J., Schie, F.W., 1992, The effect of porcine epidemic abortion and respiratory syndrome (PEARS) on sperm production. Proc 12th Int Anim Reprod Congr, pp. 1710-1712. Fennestad, K.L., Borg-Petersen, C., 1966, Experimental leptospirosis in pregnant sows. J Infect Dis 116, 57-66. Floegel, G., Wehrend, A., Depner, K.R., Fritzemeier, J., Waberski, D., Moennig, V., 2000, Detection of classical swine fever virus in semen of infected boars. Vet Microbiol 77, 109-116. Gradil, C., Dubuc, C., Eaglesome, M.D., 1996, Porcine reproductive and respiratory syndrome virus: Seminal transmission. Vet Rec 138, 521-522. Guérin, B., Pozzi, N., 2005, Viruses in boar semen: detection and clinical as well as epidemiological consequences regarding disease transmission by artificial insemination. Theriogenology 63, 556-572. Habu, A., Murakami, Y., Ogasa, A., Fujisaki, Y., 1977, Disorder of spermatogenesis and viral discharge into semen of boars infected with Japanese encephalitis virus. Uirusu 27, 21-26. Hennecken, M., Stegeman, J.A., Elbers, A.R.W., Vannes, A., Smak, J.A., Verheijden, J.H.M., 2000, Transmission of classical swine fever virus by artificial insemination during the 1997-1998 epidemic in the Netherlands: A descriptive epidemiological study. Vet Q 22, 228-233. Hinić, V., Brodard, I., Thomann, A., Cvetnić, Z., Makaya, P.V., Frey, J., Abril, C., 2008, Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems. J Microbiol Methods 75, 375-378. Kennedy, S., Moffett, D., McNeilly, F., Meehan, B., Ellis, J., Krakowka, S., Allan, G.M., 2000, Reproduction of lesions of postweaning multisystemic wasting syndrome by infection of conventional pigs with porcine circovirus type 2 alone or in combination with porcine parvovirus. J Comp Pathol 122, 9-24. Kim, J., Chae, C., 2003. Multiplex nested PCR compared with in situ hybridization for the differentiation of porcine circoviruses and porcine parvovirus from pigs with postweaning multisystemic wasting syndrome. Can J Vet Res 67, 133-137. Kim, J., Chung, H.K., Jung, T., Cho, W.S., Choi, C., Chae, C., 2002, Postweaning multisystemic wasting syndrome of pigs in Korea: prevalence, microscopic lesions and coexisting microorganisms. J Vet Med Sci 64, 57-62. Kim, J., Han, D.U., Choi, C., Chae, C., 2001, Differentiation of porcine circovirus PCV-1 and PCV-2 in boar semen using a multiplex nested polymerase chain reaction. J Virol Methods 98, 25-31. Kim, J., Han, D.U., Choi, C., Chae, C., 2003, Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested polymerase chain reaction. J Vet Med Sci 65, 741-744. Krakowka, S., Ellis, J.A., Meehan, B., Kennedy, S., McNeilly, F., Allan, G., 2000, Viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. Vet Pathol 37, 254-263. Kubista, M., Andrade, J.M., Bengtsson, M., Forootan, A., Jonák, J., Lind, K., Sindelka, R., Sjöback, R., Sjögreen, B., Strömbom, L., Ståhlberg, A., Zoric, N., 2006, The real-time polymerase chain reaction. Mol Aspects Med 27, 95-125. Lager, K.M., Mengeling, W.L., Brockmeier, S.L., 1996, Effect of postcoital intrauterine inoculation of porcine reproductive and respiratory syndrome virus on conception in gilts. Vet Rec 138, 227-228. Larochelle, R., Bielanski, A., Muller, P., Magar, R., 2000, PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. J Clin Microbiol 38, 4629-4632. Le Potier, M.F., Blanquefort, P., Morvan, E., Albina, E., 1997, Results of a control programme for the porcine reproductive and respiratory syndrome in the French “Pays de la Loire” region. Vet Microbiol 55, 355-360. Le Potier, M.F., Mesplède, A., Vannier, P., 2006, Classical Swine Fever and Other Pestiviruses, In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 309-322. Lin, M.H., Chen, T.C., Kuo, T.T., Tseng, C.C., Tseng, C.P., 2000, Real-time PCR for quantitative detection of Toxoplasma gondii. J Clin Microbiol 38, 4121-4125. Lucas, M.H., Cartwright, S.F., Wrathall, A.E., 1974, Genital infection of pigs with porcine parvovirus. J Comp Pathol 84, 347-350. Ma, W., Lager, K.M., Richt, J.A., Stoffregen, W.C., Zhou, F., Yoon, K.J., 2008, Development of real-time polymerase chain reaction assays for rapid detection and differentiation of wild-type pseudorabies and gene-deleted vaccine viruses. J Vet Diagn Invest 20, 440-447. MacMillan, A.P., Schleicher, H., Korslund, J., Stoffegen, W., 2006, Brucellosis, In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 603-611. Maes, D., Nauwynck, H., Rijsselaere, T., Mateusen, B., Vyt, P., de Kruif, A., Van Soom, A., 2008, Diseases in swine transmitted by artificial insemination: an overview. Theriogenology 70, 1337-1345. Magar, R., Larochelle, R., 2004, Evaluation of the presence of porcine reproductive and respiratory syndrome virus in pig meat and experimental transmission following oral exposure. Can J Vet Res 68, 259-266. Magar, R., Robinson, Y., Dubuc, C., Larochelle, R., 1995, Isolation and experimental oral transmission in pigs of a porcine reproductive and respiratory syndrome virus isolate. Adv Exp Med Biol 380, 139-144. Masri, S.A., Nguyen, P.T., Gale, S.P., Howard, C.J., Jung, S.C., 1997, A polymerase chain reaction assay for the detection of Leptospira spp. in bovine semen. Can J Vet Res 61, 15-20. McIntosh, K.A., Harding, J.C., Parker, S., Ellis, J.A., Appleyard, G.D., 2006, Nested polymerase chain reaction detection and duration of porcine circovirus type 2 in semen with sperm morphological analysis from naturally infected boars. J Vet Diagn Invest 18, 380-384. Medveczky, I., Szabo, I., 1981, Isolation of Aujeszky’s disease virus from boar semen. Acta Vet Acad Sci Hung 29, 29-35. Meehan, B.M., Creelan, J.L., McNulty, M.S., Todd, D., 1997, Sequence of porcine circovirus DNA: affinities with plant circoviruses. J Gen Virol 78, 221-227. Mengeling, W.L., 2006, Porcine Parvovirus, In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 373-385. Mettenleiter, T., 2000, Aujeszky’s disease (pseudorabies) virus: The virus and molecular pathogenesis-State of the art. Vet Res 31, 99-115. Montagnaro, S., Sasso, S., De Martino, L., Longo, M., Iovane, V., Ghiurmino, G., Pisanelli, G., Nava, D., Baldi, L., Pagnini, U., 2010, Prevalence of antibodies to selected viral and bacterial pathogens in wild boar (Sus scrofa) in Campania Region, Italy. J Wildl Dis 46, 316-319. Mortensen, S., Stryhn, H., Sogaard, R., Boklund, A., Stark, K.D., Christensen, J., Willeberg, P., 2002, Risk factors for infection of sow herds with porcine reproductive and respiratory syndrome (PRRS) virus. Prev Vet Med 53, 83-101. Moura, A.B., Costa, A.J., Filho, J.S., Paim, B.B., Pinto, F.R., Di Mauro, D.C., 2007, Toxoplasma gondii in semen of experimentally infected swine. Pesquisa Veterinária Brasileira 27, 430-434. Nauwynck, H., 1997, Functional aspects of Aujeszky’s disease (pseudorabies) viral proteins with relation to invasion, virulence and immunogenicity. Vet Microbiol 55, 3-11. OIE (2009). Terrestrial Animal Health Code. 2010/06/17 downloaded. http://www.oie.int/eng/normes/MCode/en_sommaire.htm Olvera, A., Sibila, M., Calsamiglia, M., Segalés, J., Domingo, M., 2004, Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J Virol Methods 117, 75-80. Pejsak, Z.K., Truszczy´nski, M.J., 2006, Aujeszky’s Disease (Pseudorabies). In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 419-433. Platt, K.B., Joo, H.S., 2006, Japanese Encephalitis and West Nile Viruses. In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 359-365. Pol, J.M., van-Dijk, J.E., Wensvoort, G., Terpstra, C., 1991, Pathological, ultrastructural, and immunohistochemical changes caused by Lelystad virus in experimentally induced infections of mystery swine disease. Vet Q 13, 137-143. Power, S.B., 1991, Diagnosing leptospira in pigs. Vet Rec 128, 43. Prieto, C., Sanchez, R., Martin-Rillo, S., Suárez, P., Simarro, I., Solana, A., Castro, J.M., 1996, Exposure of gilts in early gestation to porcine reproductive and respiratory syndrome virus. Vet Rec 138, 536-539. Prieto, C., Suárez, P., Sanchez, R., Solana, A., Simarro, I., Martin-Rillo, S., Castro, J.R., 1994, Semen changes in boars after experimental infection with porcine epidemic abortion and respiratory syndrome (PEARS) virus. Proc 13th Congr Int Pig Vet Soc, pp.98. Rose, N., Madec, F., 2007, Post-weaning multisystemic wasting syndrome in France: what we have learnt from filed epidemiology and experimental data. J Pig 59, 7-17. Rosen, L., 1986, The natural history of Japanese encephalitis virus. Ann Rev Microbiol 40, 395-414. Rossow, K.D., Bautista, E.M., Goyal, S.M., Molitor, T.W., Murtaugh, M.P., Morrison, R.B., Benfield, D.A., Collins, J.E., 1994, Experimental porcine reproductive and respiratory syndrome virus infection in one-, four-, and 10-week-old pigs. J Vet Diagn Invest 6, 3-12. Ruiz-Fons, F., Vicente, J., Vidal, D., Höfle, U., Villanúa, D., Gauss, C., Segalés, J., Almería, S., Montoro, V., Gortázar, C., 2006, Seroprevalence of six reproductive pathogens in European wild boar (Sus scrofa) from Spain: the effect on wild boar female reproductive performance. Theriogenology 65, 731-743. Schang, L.M., Kutish, G.F., Osorio, F.A., 1994, Correlation between precolonisation of trigeminal ganglia by attenuated strains of pseudorabies virus and resistance to wild-type virus latency. J Virol 68, 8470-8476. Schmoll, F., Lang, C., Steinrigl, A.S., Schulze, K., Kauffold, J., 2008, Prevalence of PCV2 in Austrian and German boars and semen used for artificial insemination. Theriogenology 15, 814-821. Segalés, J., Allan, G.M. , Domingo M., 2006, Porcine Circovirus Diseases, In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 299-307. Segales, J., Allan, G.M., Domingo, 2005, Porcine circovirus diseases. Anim Health Res Rev 6, 119-142. Segales, J., Calsamiglia, M., Olvera, A., Sibila, M., Badiella, L., Domingo, M., 2005, Quantification of porcine circovirus type 2 (PCV2) DNA in serum and tonsillar, nasal, tracheo-bronchial, urinary and faecal swabs of pigs with and without postweaning multisystemic wasting syndrome (PMWS). Vet Microbiol 20, 223-229. Segales, J., Larsens, L., Wallgren, P., Rose, N., Grau-Roma, L., Sibila, M., 2007, What do we know on epidemiology, control and prevention of porcine circovirus diseases? In: Proceedings of 5th International Symposium on Emerging and Re-emerging Pig Diseases, pp. 35-38. Shin, J., Torrison, J., Choi, C., Gonzalez, S., Crabo, B., Molitor, T., 1997, Monitoring of porcine reproductive and respiratory syndrome virus infection in boars. Vet Microbiol 55, 337-346. Soares, R.M., Durignon, E.L., Bersano, J.G., Richtzenhain, L.J., 1999, Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1. J. Virol Methods 78, 191-198. Stoddard, R.A., Gee, J.E., Wilkins, P.P., McCaustland, K., Hoffmaster, A.R., 2009, Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagn Microbiol Infect Dis 64, 247-255. Sur, J.H., Doster, A.B., Christian, J.S., Galeota, J.A., Wills, R.W., Zimmerman, J.J., Osorio, F.A., 1997, Porcine reproductive and respiratory syndrome virus replicates in testicular germ cells, alters spermatogenesis, and induces germ cell death by apoptosis. J Virol 71, 9170-9179. Swenson, S.L., Hill, H.T., Zimmerman, J.J., Evans, L.E., Landgraf, J.G., Wills, R.W., Sanderson, T.P., McGinley, J.M., Brevik, A.J., Ciszewski, D.K., Frey, M.L., 1994, Excretion of porcine reproductive and respiratory syndrome virus in semen after experimentally induced infection in boars. J Am Vet Med Assoc 204, 1943-1948. Swenson, S.L., Hill, H.T., Zimmerman, J.J., Evans, L.E., Wills, R.W., Yoon, K.J., Schwartz, K.J., Althouse, G.C., McGinley, M.J., Brevik, A.K., 1994, Artificial insemination of gilts with porcine reproductive and respiratory syndrome (PRRS) virus-contaminated semen. Swine Health Prod 2, 19-23. Swenson, S.L., Zimmerman, J., 1993, Porcine reproductive and respiratory syndrome virus in experimentally infected boars: Isolation from semen. Proc Annu Meet Am Assoc Swine Pract, pp. 719-720. Terpstra, C., Wensvoort, G., 1991, Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch’s postulates fulfilled. Vet Q 13, 131-136. Tischer, I., Gelderblom, H., Vettermann, W., Koch, M.A., 1982, A very small porcine virus with circular single-stranded DNA. Nature 295, 64-66. USDA (2009). Breeding Swine Import Protocol from European Union Recognized as Low-Risk for Classical Swine Fever. 2010/05/04 downloaded. http://www.aphis.usda.gov/import_export/animals/downloads/swine-import-pro-eu-rec-low-risk-csf.pdf Van der Linden, I.F.A., van der Linde-Bril, E.M., Voermans, J.J.M., van Rijn, P.A., Pol, J.M.A., Martin, R., Steverink, P.J.G.M., 2003, Oral transmission of porcine reproductive and respiratory syndrome virus by muscle of experimentally infected pigs. Vet Microbiol 97, 45-54. Van Oirschot, J.T., 1992, Hog Cholera. In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp.274-285. Van Rijn, P.A., Wellenberg, G.J, Hakze-van der Honing, R., Jacobs, L., Moonen, P.L., Feitsma, H., 2004, Detection of economically important viruses in boar semen by quantitative RealTime PCR technology. J Virol Methods 120, 151-160. Wills, R.W., Zimmerman, J.J., Yoon, K.J., Swenson, S.L., Hoffman, L.J., McGinley, M.J., Hill, H.T., Platt, K.B., 1997, Porcine reproductive and respiratory syndrome virus: Routes of excretion. Vet Microbiol 57, 69-81. Wittmann, G., 1985, Aujeszky’s disease: Factors important for epizootiology and control. Rec Sci Tech OIE 4, 5-20. Wittmann, G., 1986, Aujeszky's disease. Rec Sci Tech OIE 5, 959-977. Yaeger, M.J., Prieve, T., Collins, J., Christopher-Hennings, J., Nelson, E., Benfield, D., 1993, Evidence for the transmission of porcine reproductive and respiratory syndrome (PRRS) virus in boar semen. Swine Health Prod 1, 7-9. Yang, D.K., Kim, B.H., Kwon, C.H., Kwon, J.H., Lim, S.I., Han, H.R., 2004, Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea. J Vet Sci 5, 125-130. Yang, D.K., Kweon, C.H., Kim, B.H., Lim, S.I., Kim, S.H., Kwon, J.H., Han, H.R., 2004, TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus. J Vet Sci 5, 345-351. Yoon, H.A., Eo, S.K., Aleyas, A.G., Park, S.O., Lee, J.H., Chae, J.S., Cho, J.G., Song, H.J., 2005, Molecular survey of latent pseudorabies virus infection in nervous tissues of slaughtered pigs by nested and real-time PCR. J Microbiol 43, 430-436. Zimmerman, J., Benfield, D.A., Murtaugh, M.P., Osorio, F., Stevenson, G.W., Torremorell, M., 2006, Porcine Reproductive and Respiratory Syndrome Virus (Porcine Arterivirus). In: Straw, B.E., Zimmerman, J.J., Allaire, S.D., Taylor, D.J. (ed) Diseases of Swine. Blackwell, Iowa, pp. 387-417. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/33674 | - |
dc.description.abstract | 隨著人工授精 (artificial insemination; AI) 技術的引進,豬隻繁殖障礙的流行病有了重大改變。AI有防止疾病傳播、加速畜群改良以及降低生產成本等功能,然而文獻顯示由於公豬精液的病原檢測做得不夠徹底,導致疾病傳播造成龐大的經濟損失。在現今大量使用AI的情況下,為確保精液品質,需於運送前3-4小時即完成其品質的確定,故時間上的迫切性相當重要。本研究的目的為針對9種常見藉由精液傳播且影響經濟效益之重要病原,包括假性狂犬病病毒 (pseudorabies virus, PRV)、豬小病毒 (porcine parvovirus, PPV)、豬第二型環狀病毒 (porcine circovirus type 2, PCV2)、日本腦炎病毒 (Japanese encephalitis virus, JEV)、豬生殖與呼吸綜合症病毒 (porcine reproductive and respiratory syndrome virus, PRRSV)、古典豬瘟病毒 (classical swine fever virus, CSFV)、弓蟲 (Toxoplasma gondii)、鉤端螺旋體 (Leptospira spp.) 及豬布氏桿菌 (Brucella suis),建立一套具快速檢測特質的平行即時聚合酶鏈鎖反應 (multiplexed parallel real-time polymerase chain reaction, multiplexed parallel real-time PCR) 方法,以達上述精液品質確認所需。本研究使用Clontech® (Clontech laboratories, Inc., USA) 的試劑及Mastercycler® ep realplex (Eppendorf, Germany) 檢測系統,在以下條件進行反應,SYBR Adventage® qPCR premix檢測為95℃30秒,95℃5秒及60℃30秒,共45個循環;one-step qRT-PCR SYBR kit檢測為48℃20分鐘,95℃3分鐘,95℃15秒及60℃1分鐘,共45個循環,以及QTaq DNA polymerase mix檢測為95℃3分鐘,95℃15秒及60℃1分鐘,共45個循環;QTaq one-step qRT-PCR kit檢測為48℃20分鐘,95℃3分鐘,95℃15秒及60℃1分鐘,共45個循環,建立上述9種病原之平行方式同步偵測技術;其中包括以SYBR Green作為螢光訊號的來源,配合real-time PCR產物裂解溫度 (melting temperature; Tm) 的不同,進行引子對可行性的確認及單一病原偵測技術的建立,進而再以TaqMan在增幅片段上加入特定探針 (probe),利用不同波長的螢光偵測,發展可同時檢測多種病原之快速檢測方法,並針對其特異性和靈敏度進行分析,以確認其檢測之穩定性及再現性。本研究針對上述9種病原所建立之方法,SYBR Green系統偵測靈敏度以PPV和Leptospira spp.的17.6及19.6 copies/μL為最佳,其餘為101~2 copies/μL,而TaqMan系統以JEV的3.6 copies/μL為最佳,其餘為101 copies/μL。上述方法之建立,僅需耗時3~4小時即可快速檢測豬隻精液以確保其品質,進而達到豬隻繁殖障礙疾病的防控,提升配種受胎率和經濟產能,以而降低豬場和國家的經濟損失。 | zh_TW |
dc.description.abstract | The epidemiology of swine reproductive diseases has changed significantly following the introduction of artificial insemination (AI). The advantages of AI include prevention of disease transmission, breeding improvement, cost reduction, and overcoming the impediment in natural mating. However, it has also been shown more serious disease spreading and economic loss can occur due to incomplete pathogen inspection in the semen used for AI. Under the current high demanding of AI in pig industry, it is required that each semen sample used for AI needs to be thoroughly examined 3-4 hours before shipping to ensure its quality. Therefore, the speed for semen examination is critical. In the present study, a high throughput multiplexed, parallel real-time polymerase chain reaction (rt-PCR) was established to simultaneously detect 9 important reproductive pathogens, including pseudorabies virus (PRV), parvovirus (PPV), porcine circovirus type 2 (PCV2), Japanese encephalitis virus (JEV), porcine reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus (CSFV), Toxoplasma gondii, Leptospira spp., and Brucella suis, in swine semen. Reagents of Clontech® (Clontech laboratories, Inc., USA) and Mastercycler® ep realplex (Eppendorf, Germany) were used for the establishment of the parallel rt-PCR for the 9 pathogens, under the following conditions: initial denaturation at 95 ℃ for 30 sec, 45 cycles of denaturation at 95 ℃ for 5 sec, and annealing and extension at 60 ℃ for 30 sec of SYBR Adventage® qPCR premix; initial reverse transcription at 48 ℃for 20 min, followed by initial denaturation at 95 ℃ for 3 min, 45 cycles of denaturation at 95℃ for 15 sec, and annealing and extension at 60 ℃for 1 min of one-step qRT-PCR SYBR kit; furthermore, initial denaturation at 95 ℃for 3 min, 45 cycles of denaturation at 95 ℃ for 15 sec, and annealing and extension at 60 ℃ for 1 min of QTaq DNA polymerase mix; initial reverse transcription at 48 ℃for 20 min, followed by initial denaturation at 95 ℃ for 3 min, 45 cycles of denaturation at 95℃ for 15 sec, and annealing and extension at 60 ℃for 1 min of QTaq one-step qRT-PCR kit. Two fluorescence detection methods for monitoring rt-PCR were included. The dsDNA-binding dye SYBR Green I was used along with the analysis of the melting temperature (Tm) of the amplified products for primer validation and singleplex condition optimization. TaqMan probes were then designed so that they could anneal within a DNA region amplified by a specific set of primers to develop a rapid multiplexed, parallel rt-PCR for the detection of the 9 above mentioned pathogens simultaneously. Furthermore, analysis of the specificity and sensitivity to validate the stability and reproducibility of each assay was performed. The optimal detection limit for SYBR Green was 17.6 copies/μL for PPV, 19.6 copies/μL for Leptospira spp., and 101~2copies/μL for others. The optimal detection limit for TaqMan was 3.6 copies/μL for JEV and 101copies/μL for others. The established multiplexed parallel rt-PCR could check the 9 target pathogens simultaneously and rapidly which could ensure the completion of quality examination of semen within 3-4 hours and prevent the spread of reproductive pathogens via semen in the future. The improved semen quality control for AI will effectively enhance the reproduction performance in pig industry and reduce the associated economic loss. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T05:44:27Z (GMT). No. of bitstreams: 1 ntu-100-R98629022-1.pdf: 2264526 bytes, checksum: e1ae12e9521b6c53e3a54df55baf6072 (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員會審定書……………………………………………….……ii
致謝…………………………………………………………...………iii 中文摘要……………………………………………………………….iv 英文摘要……………………………………………………………….vi 目錄. ………………………………………………………………….ix 圖次……………………………………………………………………xiii 表次……………………………………………………………………xiv 附錄……………………………………………………………………xv 第一章 序言………………………………………………………1 第二章 文獻回顧…………………………………………………3 第一節 豬隻繁殖疾病之重要病原………………………………3 2.1.1 假性狂犬病病毒………………………………………….…3 2.1.2 豬小病毒…………………………………………………….4 2.1.3 豬第二型環狀病毒…………………………………….……5 2.1.4 日本腦炎病毒…………………………………………….…6 2.1.5 豬生殖與呼吸綜合症病毒………………………………….7 2.1.6 古典豬瘟病毒……………………………………….…..…9 2.1.7 弓蟲………………………………………………...……..10 2.1.8 鉤端螺旋體……………………………………….....……10 2.1.9 豬布氏桿菌………………………………………...………11 第二節 世界動物衛生組織 (OIE) 及其他養豬國家對公豬精液的防疫檢疫規範…………………..................………………11 2.2.1 世界動物衛生組織(OIE)對於提供精液之公豬的相關規範...………………………………………………………………......…12 2.2.2 世界動物衛生組織(OIE)對於提供精液之公豬在布氏桿菌病的相關規範……………………………………………………………….12 2.2.3 世界動物衛生組織(OIE)對於提供精液之公豬在假性狂犬病的相關規範……………………………………………………………….13 2.2.4 世界動物衛生組織(OIE)對於提供精液之公豬在豬瘟的相關規範………………………………………………………………..…...13 2.2.5 其他養豬國家對公豬精液的相關檢疫規範…………………14 2.2.5.1 美國對公豬精液的相關檢疫規範………………………..14 2.2.5.2 澳洲對公豬精液的相關檢疫規範………………………..14 2.2.5.3 台灣對公豬精液的相關檢疫規範………………………..15 第三節 單一及平行即時聚合酶鏈鎖反應在精液安全的應用……15 2.3.1 即時聚合酶鏈鎖反應之原理及應用…………………………16 2.3.1.1 SYBR Green real-time PCR之原理……………………..17 2.3.1.2 TaqMan real-time PCR之原理………………………...17 第三章 材料與方法……………………………………….…….19 第一節 實驗設計……………………………………………….19 第二節 實驗材料…………………………………………………20 3.2.1 標準病原株取得……………………………………….....20 3.2.2 即時聚合酶鏈鎖反應之儀器及商品化試劑……………...20 3.2.2.1 Real-time PCR儀器及反應管……………………..…..20 3.2.2.2 商品化SYBR Green試劑………………………….………20 3.2.2.3 商品化TaqMan試劑………………………………….……21 3.2.3 臨床精液樣本……………………………………………….21 第三節 實驗方法…………………………………………………21 3.3.1 病原菌之核酸萃取…………………………………………21 3.3.2 引子對及探針之製備……………………………….……22 3.3.3 微量質體 DNA 製備………………………………………23 3.3.3.1 質體DNA轉殖和培養……………………………………....23 3.3.3.2 質體DNA萃取………………………………………………24 3.3.3.3 質體DNA之酵素切割………………………………………24 3.3.4 利用SYBR Green進行單一及平行即時聚合酶鏈鎖反應……25 3.3.5 利用TaqMan進行單一及平行即時聚合酶鏈鎖反應…………26 3.3.6 引子及探針濃度調整…………………………………………27 3.3.6.1 靈敏度測試…………………………………………………27 3.3.6.2 特異性測試…………………………………………………28 3.3.7 數據分析………………………………………………………28 3.3.7.1 標準曲線……………………………………………………28 3.3.8 統計分析………………………………………………………29 第四章 結果…………………………………………………….…30 第一節 單一病原即時聚合酶鏈鎖反應之條件建立………………30 4.1.1 假性狂犬病病毒…………………………………………….…30 4.1.2 豬小病毒………………………………………………………31 4.1.3 豬第二型環狀病毒………………………………………….32 4.1.4 日本腦炎病毒………………………………………..…….32 4.1.5 豬生殖與呼吸綜合症病毒………………………………..34 4.1.6 古典豬瘟病毒……………………………………………….34 4.1.7 弓蟲…………………………………………………………..35 4.1.8 鉤端螺旋體…………………………………………………..36 4.1.9 豬布氏桿菌…………………………………………………..37 4.1.10各病原最佳化條件之特異性分析…………………………….39 第二節 臨床樣本核酸檢測…………………………………………39 4.2.1 Singleplex real-time (RT-)PCR檢測結果及分析………40 4.2.2 Parallel real-time (RT-)PCR檢測結果及分析……………41 4.2.3 SYBR Green/ TaqMan real-time (RT-)PCR檢測之再現性分析...................................................…...42 第五章 討論…………………………………………………………43 第六章 參考文獻……………………………………………………50 圖次 圖1 PRV進行SYBR Green real-time PCR反應數據分析…………64 圖2 PRV引子對及探針最佳化濃度測試……………………………65 圖3 PRV進行singleplex TaqMan real-time PCR反應的靈敏度測試及標準曲線的增幅效率分析……………….................……66 圖4 JEV進行SYBR Green one-step real-time RT-PCR反應數據分析…….…............................................…..67 圖5 JEV引子對及探針最佳化濃度測試……………………………….…….....................................................68 圖6 JEV探針最佳化濃度測試………………………………………...…..................................................…….69 圖7 JEV進行singleplex TaqMan one-step real-time RT-PCR反應的靈敏度測試及標準曲線的增幅效率分析……………………..….70 圖8 Toxoplasma gondii進行SYBR Green real-time PCR反應數據分析.……............................................…..71 圖9 Toxoplasma gondii引子對及探針最佳化濃度測試………….72 圖10 Toxoplasma gondii進行singleplex TaqMan real-time PCR 反應的靈敏度測試及標準曲線的增幅效率分析………………………73 圖11 Leptospira spp.進行SYBR Green real-time PCR反應數據分析…...........................................…..…...…74 圖12 Leptospira spp.引子對及探針最佳化濃度測試……………75 圖13 Leptospira spp.進行singleplex TaqMan real-time PCR 反應的靈敏度測試及標準曲線的增幅效率分析...................76 圖14 Brucella suis進行SYBR Green real-time PCR反應數據分析….……............................................……77 圖15 Brucella suis引子對及探針最佳化濃度測試………………78 圖16 Brucella suis進行singleplex TaqMan real-time PCR 反應的靈敏度測試及標準曲線的增幅效率分析………….........……79 圖17 Toxoplasma gondii進行SYBR Green real-time PCR引子對特異性分析….............................................…80 表次 表1 各病原引子對及探針序列………………………………………81 表2 Real-time (RT-)PCR之SYBR Green kit反應試劑與條件……82 表3 Real-time (RT-)PCR之QTaq (TaqMan) kit反應試劑與條件…83 表4 利用Singleplex real-time (RT-)PCR檢測臨床精液樣本PRV、PPV、PCV2、JEV、PRRSV、CSFV、Toxoplasma gondii、Leptospira spp.及Brucella suis 九種病原之核酸結果及分析…………………84 表5 利用Parallel real-time (RT-)PCR檢測臨床精液樣本PRV、PPV、PCV2、JEV、PRRSV、CSFV、Toxoplasma gondii、Leptospira spp.及Brucella suis 九種病原之核酸結果及分析…………………85 表6 利用Singleplex real-time (RT-)PCR檢測單管臨床精液樣本共有幾種病原之檢出結果………………………………..............86 表7 SYBR Green/ TaqMan real-time (RT-)PCR最佳化條件之彙整…………..............................................…86 表8 Singleplex TaqMan real-time (RT-)PCR最佳化條件………87 表9 Singleplex SYBR Green real-time (RT-)PCR之專一Tm值分布…………...............................................87 附錄 附錄一 中南部地區 71 個豬隻精液檢測PRV、PPV、PCV2、Toxoplasma gondii、Leptospira spp.以及Brucella suis之結果…………………………….…...........................…88 附錄二 中南部地區 71 個豬隻精液檢測JEV、PRRSV以及CSFV之結果…..................................................…92 | |
dc.language.iso | zh-TW | |
dc.title | 最佳化之平行即時聚合酶鏈鎖反應以診斷經由精液傳播之豬繁殖性疾病 | zh_TW |
dc.title | Establishment of Optimized Parallel Real-time PCR for the Diagnosis of Semen-Transmitted Swine Reproductive Diseases | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 蕭世烜(Shih-Hsuan Hsiao),鄭謙仁(Chian-Ren Jeng) | |
dc.contributor.oralexamcommittee | 劉涓(Jiuan Judy Liu),張志成(Chih-Cheng Chang) | |
dc.subject.keyword | 平行即時聚合酶,鏈鎖反應,豬隻繁殖障礙疾病,人工授精,SYBR Green,TaqMan, | zh_TW |
dc.subject.keyword | parallel real-time polymerase chain reaction,pig reproductive disorders,artifi,cial insemination,SYBR Green,TaqMan, | en |
dc.relation.page | 94 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-07-27 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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