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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 潘建源 | |
dc.contributor.author | Po-Yuan Shih | en |
dc.contributor.author | 施博原 | zh_TW |
dc.date.accessioned | 2021-06-13T01:06:55Z | - |
dc.date.available | 2012-07-26 | |
dc.date.copyright | 2007-07-26 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-23 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29432 | - |
dc.description.abstract | Calneuron具有兩個可以和鈣離子結合的EF-hand motif,且在其C端有一段疏水性的結構。本論文中,我們利用PCR技術選殖出大鼠的calneuron,並將其表現在HEK293t細胞中,發現分布位置主要是在細胞膜附近;而缺乏疏水性C-端的變異(Cal | zh_TW |
dc.description.abstract | Calneuron has a structure similar to calmodulin-like proteins but has only two conserved N-terminal EF-hand motifs and an extent C-terminal hydrophobic tail. The expression of calneuron was specific in neuronal tissues and higher in adult than in fetal rat cortex. When expressed in HEK293t cells, calneuron was localized at the plasma membrane, but changed to the cytosol for the mutant without the C-terminal hydrophobic tail (Cal | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T01:06:55Z (GMT). No. of bitstreams: 1 ntu-96-R94b41022-1.pdf: 2308495 bytes, checksum: b1aa90a6ad59267e2a2594179ae4f0d7 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | I. ABSTRACTS
I.1 English abstract………………………………………………………………...1 I.2 Chinese abstract………………………..………………………………………3 II. INTRODUCTION II.1 Voltage-gated calcium channels……………………………………………….4 II.2 Calcium-binding protein family…………………………….………………...6 II.3 SCY-1 like protein is predicted as Serine/Threonine protein kinase.................8 II.4 CaMKII is important in neuronal functions.………………………………….9 II.5 Our aims………………………………………………………………….…..11 III. METHODS AND MATERIALS III.1 Chemicals and reagents……………………………………...………….......12 III.2 Solutions………………………………………………………………….…14 III.3 RNA extraction and cDNA synthesis.............................................................17 III.4 Polymerase chain reaction and agarose gel electrophoresis...…………..…..18 III.5 TA cloning…………………………………………………………………..18 III.6 Plasmid purification………………………………………………………...19 III.7 Expression vector construction……………………………………………..19 III.8 Primary bovine chromaffin cell culture……………………………………..19 III.9 Transient expression………………………………………………………...20 III.10 Fluorescent imaging…………………………………………………….....21 III.11 Calcium imaging……………...……………………………………………22 III.12 Electrophysiology………………………………………………………….23 III.13 Yeast small scale transformation..………………………………………....24 III.14 Western immunoblot analysis………………………………………….......24 III.15 3-AT selection………………………….……………………………….….25 III.16 Yeast large scale library transformation.…………………………………..25 III.17 Yeast plasmid purification………………………………………………....26 III.18 Yeast colony identification………………………………………………...27 III.19 Data analysis…………………………………………………………….…27 IV. RESULTS IV.1 Calneuron is highly homologous to other CaBPs……………………...........28 IV.2 Calneuron has two functional EF-hand motifs and a hydrophobic C-terminal…………………………………………………………………..28 IV.3 Calneuron is a neuron-specific protein………………………...…………....29 IV.4 Calneuron is a membrane-associated protein……………………….………29 IV.5 Calneuron attenuates the depolarization-evoked calcium signaling response………………………………………………………………….....30 IV.6 Calneuron inhibits the calcium but not sodium currents………………........31 IV.7 Calneuron does not change the kinetics of sodium currents…………….......32 IV.8 C-terminal hydrophobic tail is important for calcium current modulation….33 IV.9 Calneuron inhibits N-type calcium currents…..……………………...…….34 IV.10 Effects of calneuron are not the influences of fusion protein..………...….35 IV.11 Calneuron inhibits activation and inactivation of L-type calcium current instead of amplitude……………………………………………..................36 IV.12 L-type calcium current is major component of remained calcium currents in chromaffin cells…………………………………………………….............37 IV.12 L-type calcium current is major component of remained calcium currents in chromaffin cells 37 IV. 13 Calneuron may interact with CaMKJIf3 and SCY- 1 like proteins 38 IV. 14 Interactions are not shared by other CaM-like proteins 39 IV.15 Calneuron shows similar localization with CaMKJIf3 but not CaMKJIa. . .40 V. DISCUSSION V.1 Calneuron regulates calcium channels activities and signaling pathway 41 V2 Calcium channel modulation is important for neuron activities 43 V3 Roles of C-terminal hydrophobic tail 45 V.4 Relationship between calneuron and CaMKJIf3 48 V.5 Summary and future directions 50 VI.REFERENCES 51 VII.TABLE 58 VIII.FIGURES 59 | |
dc.language.iso | en | |
dc.title | Calneuron調控鈣離子訊息傳遞的研究 | zh_TW |
dc.title | Effects of Calneuron in Modulating Calcium Signaling Pathway | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 閔明源,陳建璋,林崇智 | |
dc.subject.keyword | 鈣離子通道, | zh_TW |
dc.subject.keyword | calneuron,Ca2+ channel,CaMKII,patch clamp, | en |
dc.relation.page | 74 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-23 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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