請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24459
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳建源(Chien-Yuan Chen) | |
dc.contributor.author | Tai-Pang Chen | en |
dc.contributor.author | 陳泰邦 | zh_TW |
dc.date.accessioned | 2021-06-08T05:26:49Z | - |
dc.date.copyright | 2005-07-28 | |
dc.date.issued | 2005 | |
dc.date.submitted | 2005-07-19 | |
dc.identifier.citation | 1. 張振堶, '世界感測器在新興應用領域市場的成長分析', 工研院量測中心(2000).
2. L.C. Clark Jr and C. Lyons, Electrode system for continuous monitoring in cardiovascular surgery, Ann. N.Y. Acad. Sci. 102 (1962) 29-45. 3. F. Palmisano, P.G. zambonin, D. Centonze, Amperometric biosensors based on electrosynthesised polymeric films, Fresenius J Anal Chem 366 (2000) 586-601 4. A.A. Shul’ga, M. Koudelka-Hep, N.F. de Roolj, Glucose-sensitive enzyme field effect transistor using potassium ferricyanide as an oxdizing substrate, Anal. Chem. 66(1994) 205-210. 5. Y. Miyahara, T.Maruzumi, S. Shiokawa, H. matsuoka, I. Karube, S. Suzuki, Micro urea seneor using semiconductor and enzyme immobilizing technologies, Chem. Soc. Japan 6 (1983) 823-830. 6. Y. Hanazato, Ken-Ichi Inatomi, M. Nakako, S. Shiono and M. Maeda, Glucose-sensitive field-effect transistor with a membrane containing co-immobilized gluconolactonase and glucose oxidase, Anal. Chim. Acta 212 (1988) 49-59. 7. S.V. Dzyadevych, J.M. Chovelon, A comparative photodegradation studies of methyl pasathion by using Lumistox test and conductometric biosensor technique, Materials Science and Engineering C 21 (2002) 55-60. 8. S.V. Dzyadevych, A.P. Soldatkin, J.M. Chovelon, Assessment of the toxicity of methyl parathion and its photodegradation products in water samples using conductometric enzyme biosensors, Anal. Chim. Acta 459 (2002) 33-41. 9. S.V. Dzyadevych, V.N. Arkhypova, Y.I.Korpan, A.V. El/skaya, A.P. soldatkin, N.jaffrezic-Renault, C. Martelet, Conductometric formaldehyde sensitive biosensor with specifically adapted analytical characteristics, Anal. Chim. Acta 445 (2001) 47-55. 10. D. Shaojun, D. Qing and C. Guangjin, Cholesterol sensor based on electrodeposition of catalytic palladium particles, Anal. Chim. Acta 279 (1993) 235-240. 11. B. Xie, K. Ramanathan, B. Danielsson, Mini/micro thermal biosensors and other related devices foor biochemical/clinical analysis and monitoring, Trends in Anal. Chem. 19 (2000) 340-349. 12. A. Dittmar, Ph. Roussel, G. Delhomme, D. Barbier, V. Lysenko, V. Rossokhaty, V. Strikha, Oxidized porous silicon:A new approach in support thermal isolation of thermopile-based biosensors, Sensors And Actuators A 67 (1998) 205-210. 13. Z. Chao, F. Guanping, G. Zhixian, Development of a new kind of dualmodulated QCM biosensor, Biosens. Bioelectro. 12 (1997) 1219-1225. 14. Witz, Jean, Kinetic Analysis of Analyte Binding by Optical Biosensors:Hydrodynamic Penetration of the Analyte Flow into the Polymer Matrix Reducesthe Influence of Mass Transport, Analytical Biochemistry 270:2 (1999) 201-206. 15. Myszka, G. David, A. Thomas, L. Michael, Chaiken, M. Irwin, Kinetic analysis of a protein antigen-antibody interaction limited by mass transporton an optical biosensor, Biophysical Chemistry 64 (1997) 127-137. 16. Rickert, Jan; Brecht, Andreas; Göpel, Wolfgang, Quartz crystal microbalances for quantitative biosensing and characterizing protein multilayers, Biosens. Bioelectro. 12:7 (1997) 567-575. 17. C.G marxer, M.C. Coen, H. Bissig, U.F. Greber, L. Schlapbach, Simultaneous measurement of the maximum oscillation amplitude and the transient decay time constant of the QCM reveals stiffness changes of the adlayer, Anal. Bioanal. Chem. 377 (2003) 570-577. 18. R.J. Geise, S.Y. Rao, A.M. Yacynch, Electropolymerized 1,3-diaminobenzene for the construction of a 1,1’-dimethylferrocene mediated glucose biosensors. Anal. Chim. Acta 281 (1993) 467-473. 19. L. Ying, Fabrication of a carbon paste electrode and its application to develop a biosensor (1998) 20. Y.Mishima, J. Maruyama, I. Nakabayashi, S. Ikeda, Glucose sensor based on titanium dioxide electrode modified with potassium hexacyanoferrate(III), Sens. Actuators B 65 (2000) 343-345. 21. I.L. Mattos, L. Gorton, T. Laurell, A. Malinauskas, A.A. Karyakin, Development of biosensors based on hexacyanoferrates, Talanta 52 (2000) 791-799. 22.J. Kulys, The carbon paste electrode encruster with a microreactor as glucose biosensors, Biosens. Bioelectro. 14 (1999) 473-479. 23.A. Silber, N. Hampp, W. Schuhmann, Poly(methylene blue)-modified thick-film gold electrodes for the electrocatalytic oxidation of NADH and their application in glucose biosensors, Biosens. Bioelectron. 11 (1996) 215-223. 24. E. Ohasshi, I. Karube, Development of a thin membrane gluc 25.A.E. Cass, Davis, Francis, Scott, etc., Ferrocene-mediated enzyme electrode for amperometric determination of glucose, Anal. Chem. 56 (1984) 667-671. 26. S. Wei Chung, Development of lipid panel biosensors (2004) 27. T. Nakaminami, I. Shin-ichiro, S. Kuwabata, H. Yoneyama, Amperometric determination of total cholesterol at gold electrodes covalently modified with cholesterol oxidase and cholesterol esterase with use of thionin as an electron mediator, Anal. Chem. 71 (1999) 1068-1076 28. Y. Minghui, Y. Yunhui, Y. Yu, S. Guoli, Y.Ruqin, Bienzymatic amperometric biosensor for choline based on mediator thionine in situ electropolymerized within a carbon paste electrode, Anal. Biocem. 334 (2004) 127-134. 29. A. Schulte, R.H. Chow, Cylindrically etched carbon-fiber microelectrodes for low-noise amperometric recording of cellular secretion, Anal. Chem. 70 (1998) 985-990. 30. S. Moane, J.R. Rodriguez, B. Ordieres, A.J.M.,P.T. Smyth, Electrochemical behavious of clenbuterol at Nafion-modified carbon-paste electrode, Journal of Pharamacetical and Biomedical Analysis. 14 (1995) 57-63. 31. H.C. Shu, L. Gorton, B. Persson, B. Mattiasson, A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD+ , and mediator containing polymer for D-latic acid analysis II. On line monitoring of fermentation process, Biotechnology and Bioengineering 46 (1994) 280-284. 32. T. Ikeda, Hamada, H.Miki, M. Senda, Glucose oxidase-immobilized benzoquinone-carbon paste electrode as a glucose sensor, Agric. Biol. Chem. 49:2 (1985) 541-543. 33.H.C. Shu, B. Mattiasson, B. Persson, G. Nagy, L. Gortin, S. Sahni, L. Geng, T. Skotheim, A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD+ , and mediator containing polymer for D-latic acid analysis. I. Construction, composition and characterization, Biotechnol. Bioeng. 46 (1995) 270-279. 34. H.C. Shu, B. Mattiasson, On-line monitoring of D-lactic acid during a fermentation processing using immobilized D-lactate dehydrogenase in a sequential injection analysis, Anal. Chem. Acta. 300:1-3 (1995) 277-285. 35. J. Marcinkeviciene, J. Kulys, Bienzyme strip-type glucose sensor, Biosens. Bioelectron. 8 (1993) 209-212. 36. W.A. Collier, D. Janssen, A.L. Hart. Merasurement od soluble L-lactate in dairy products using screen-printed sensors in batch mode, Biosens. Bioelectron. 11:10 (1996) 1041-1049. 37. A.L Hart, H. Cox, D. Janssen, Stabilization of lactate oxidase in screen-printed enzyme electrodes, Biosens. Bioelectron. 11:8 (1996) 833-837. 38. D.R. Matthews, R.R Holman, E. Bown, J. Steemson, A. Watson, S. Hughes, D. Scott, Pen-sized digital 30-second blood glucose meter,The Lancet 4 (1987) 778-779. 39. M. Albareda, A. Merkoçi, S. Alegret, Configurations used in the design of screen-printed enzymatic biosensors, Sensors and Actuators B: Chemical 69 (2000) 153-163. 40. 蘇麗華,「五鼎以血糖機、檢驗試片挑戰不景氣」,生技時代 (2001) 90-93. 41. 鍾協訓,曾志明,「網版印刷電極在分析化學上的製作與應用」,科儀新知,第二十二卷第三期 (2000) 72-73 42. C.A. Galán-Vidal, J. Muñoz, C. Domínguez, S. Alegret, Chemical sensors, biosensors and thick-film technology, Trend in Analytical Chemistry 14 (1995) 225-231 43. L. Stryer, Biochemistry. W. H. Freeman and Company, New York. (1995). 44. 何敏夫, 臨床化學-原理與實驗 (1996) 合記圖書出版社。 45. http://www.medscape.com 46. 賴亮全、林則彬、林富美合譯, 蓋統生理學-生理及疾病機轉 (1998)華杏出版社。 47. D.J. McNamara, Dietary cholesterol and atherosclerosis, Biochimicaet Biiophysica Acta 1529 (2000) 310-320. 48. G.D. Sloop, A critical analysis of the role role of cholesterol in atherogenesis, Atherosclerosis 142 (1999) 265-268. 49. N.J. Wald, M.R. Law, Serum cholesterol and ischaemic heart disease, Atherosclerosis 118 (1995) S1-S5. 50. W.B. Kannel, Range of Serum Cholesterol Values in the Population Developing Coronary Artery Disease, The American Journal of Cardiology 76 (1995) 69-77. 51. Toyobo enzymes ,TOYOBO CO., LTD. Japan. (2002-2003) 52. C. Allain, S. Poon, C. Fu, Enzymatic determination of total serum cholesterol, Clin. Chem. 20:4 (1974) 470-475. 53. M. A. T Gilmartin,.J.P. Hart, Fabrication and characterization a screen-printed, disposable, amperometric cholesterol biosensor, Analyst 119 (1994) 2331-2336. 54. J. Motonaka, L.R. Faulkner, Determination of cholesterol and cholesterol ester with novel enzyme microsensors, Anal. Chem. 65 (1993) 3258-3261. 55 B. Raguse, B.A. Cornell, L.G. King, L. Wieczorek. Tethered lipid bilayer membranes:Formation and ionic reservoir characterization, Langmuir. 14 (1997) 648-659. 56. P.H. Lolekha, P. Srisawasdia, P. Jearanaikoon, N. Wetprasit, B. Sriwanthana, M.H. Kroll, Performance of four sources of cholesterol oxidase for serum cholesterol determination by the enzymatic endpoint method, Clin. Chim. Acta 339 (2004) 135–145. 57. http://www.health4ever.com/lab/lab_topic01.asp ose sensor using | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/24459 | - |
dc.description.abstract | 人體血液中總膽固醇的含量是判斷體內脂質代謝是否異常的指標,過高可能有冠狀動脈硬化、糖尿病或甲狀腺機能減退等徵兆;過低亦有貧血、憂鬱症或罹病率、死亡率高的可能。
本研究主要目的是開發一電化學式生物感測器用以檢測血清中總膽固醇。先以自製碳漿電極對各種電子傳遞媒介物以循環伏安法(cyclic voltammetry)及電流法(amperometry)來評估其效用及進行電極之電化學處理,實驗結果顯示自製之碳漿電極配合三種不同的電子傳遞媒介物皆可有效降低檢測 H2O2 之工作電壓,並選擇以K3Fe(CN)6 作為後續試驗。後續實驗以市售網版印刷技術製作之可拋棄式平版碳漿電極(screen-printed carbon paste electrodes),配合膽固醇氧化酶(ChOx)、膽固醇酯酶(ChE)及電子傳遞媒介物K3Fe(CN)6共同構成檢測系統。實驗中各酵素及電子傳遞媒介物以物理性吸附的方式固定於電極表面,並探討系統最適操作條件,分別選定操作電位為 0.2 V、pH 7.0 、7.5 mM K3Fe(CN)6、1.2 % Triton X-100、1.0 Unit ChOx及1.0 Unit ChE。於室溫下操作,可測得膽固醇線性範圍至333.3 mg/dl,R2=0.995,而可測得膽固醇酯線性範圍至 100 mg/dl,R2=0.951。此生物感測器所檢測的線性範圍可涵蓋血液中游離膽固醇之正常範圍。 | zh_TW |
dc.description.abstract | Determination of serum cholesterol has diagnostic implications:an increase in its level is associated with coronary artery disease, nephrosis, diabetes, myxoedema, obstructive jaundice and hypothyroidism, whereas a decreased level of cholesterol is observed in cases of hypothyroidism, anaemia, malabsorption and wasting syndromes. It is desired to develop techniques which allow convenient and rapid determinations of cholesterol.
The aim of this study was to develop disposable planar electrodes apply to cholesterol and cholesteryl ester detection. The feasibility of the fabricated electrodes was evaluated by electrochemical methods. The current responses were proportional to concentrations of electroactive substances, hydrogen peroxide. Three mediators:iron(II,III) oxide, thionin and potassium ferricyanide had been tested using cyclic voltammetry and amperometric method to detect hydrogen peroxide. Therefore, potassium ferricyanide was chosen as a mediator to use in planar electrode. To achieve a reagentless biosensor, potassium ferricyanide and cholesterol oxidase and cholesterol esterase were co-immobilized onto the electrode surface by adsorption. The optimal operation condition for total cholesterol detection was at 0.2 V potential in 0.02 M pH 7.0 phosphate buffer, 1.2% Triton X-100 and 7.5 mM potassium ferricyanide, 1.0 Unit of cholesterol oxidase and 1.0 Unit of cholesterol esterase. The sensor has a good linear response in the range 0~333.3 mg/dl for determination of cholesterol and 0~100 mg/dl for determination of cholesteryl ester. The range can include the normal value of serum free cholesterol. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T05:26:49Z (GMT). No. of bitstreams: 1 ntu-94-R92b47407-1.pdf: 1984803 bytes, checksum: 42797e5770c2c4b9eb8368c0c0da7311 (MD5) Previous issue date: 2005 | en |
dc.description.tableofcontents | 目錄------------------------------------------------------Ⅰ
圖目錄----------------------------------------------------Ⅳ 表目錄----------------------------------------------------Ⅶ 中文摘要--------------------------------------------------Ⅷ 英文摘要--------------------------------------------------Ⅸ 第一章 緒論 1.0 實驗背景------------------------------------ 1 1.1 生物感測器---------------------------------- 3 1.1.1 生物感測器之構成元件------------------------ 3 1.1.2 生物感測器之種類---------------------------- 4 1.1.2.1 電化學型生物感測器-------------------------- 4 1.1.2.2 光學型生物感測器---------------------------- 7 1.1.2.3 熱學型生物感測器---------------------------- 8 1.1.2.4 聲學型生物感測器---------------------------- 8 1.1.3 優良生物感測器需具備之要件------------------ 9 1.1.3.1 辨識元固定技術------------------------------10 1.1.3.2 干擾排除之技術------------------------------12 1.1.4 電子傳遞媒介物------------------------------12 1.1.5 碳漿電極------------------------------------13 1.1.6 網版印刷技術--------------------------------15 1.2 膽固醇與膽固醇酯----------------------------16 1.2.1 膽固醇之生合成------------------------------16 1.2.2 膽固醇與膽固醇酯之結構----------------------19 1.2.3 膽固醇在體內的運輸與代謝--------------------20 1.2.4 膽固醇與血管硬化及心臟病之關係--------------22 1.2.5 膽固醇氧化酶與膽固醇酯酶簡介----------------22 1.2.5.1 膽固醇氧化酶--------------------------------22 1.2.5.2 膽固醇酯酶----------------------------------22 1.2.6 膽固醇分析法的發展--------------------------23 1.3 實驗原理------------------------------------25 1.4 實驗目的------------------------------------25 1.5 實驗架構------------------------------------25 第二章 材料與方法 2.0 實驗藥品與器材------------------------------26 2.1 碳漿電極之製作方法--------------------------26 2.2 Thionin 修飾之碳漿電極----------------------28 2.3 Fe3O¬4 修飾之碳漿電極-------------------------28 2.4 酵素之固定化方法----------------------------29 2.5 電化學實驗方法------------------------------30 2.6 感測器操作之方法----------------------------31 2.7 以呈色法進行膽固醇氧化酶活性之分析----------32 第三章 結果與討論 3.1 自製碳漿電極--------------------------------34 3.1.1 空白碳漿電極--------------------------------34 3.1.2 Thionin 修飾之碳漿電極----------------------35 3.1.3 Fe3O¬4 修飾之碳漿電極-------------------------39 3.1.4 空白碳漿電極對K3Fe(CN)6之探討---------------44 3.2 平版碳漿電極--------------------------------49 3.2.1 平版碳漿電極之前處裡------------------------49 3.2.2 基礎性質之探討------------------------------51 3.2.3 檢測游離膽固醇------------------------------53 3.2.3.1 循環伏安法----------------------------------53 3.2.3.2 最適 K3Fe(CN)6-------------------------------57 3.2.3.3 最適膽固醇氧化酶添加量----------------------57 3.2.3.4 最適操作 pH---------------------------------57 3.2.3.5 最適 Triton X-100 添加量--------------------60 3.2.3.6 以最適化條件檢測游離膽固醇¬------------------60 3.2.4 檢測膽固醇酯--------------------------------64 3.2.5 血液中干擾物質探討--------------------------68 3.3 酵素呈色法檢測游離膽固醇--------------------68 第四章 結論 4.1 結論----------------------------------------72 4.2 未來展望------------------------------------72 參考文獻 ¬----------------------------------------------------73 | |
dc.language.iso | zh-TW | |
dc.title | 以複合酵素系統配合碳漿電極開發電化學式總膽固醇生物感測器 | zh_TW |
dc.title | Development of an electrochemical biosensor of total cholesterol by the combination of a multienzymatic system and a carbon paste electrode | en |
dc.type | Thesis | |
dc.date.schoolyear | 93-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉雨田,許文林,周淑芬,張谷昇 | |
dc.subject.keyword | 碳漿電極,膽固醇,生物感測器,電化學, | zh_TW |
dc.subject.keyword | carbon paste electrode,cholesterol,biosensor,electrochemical, | en |
dc.relation.page | 76 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2005-07-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-94-1.pdf 目前未授權公開取用 | 1.94 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。