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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 蕭世烜 | |
dc.contributor.author | Wen-Ta Li | en |
dc.contributor.author | 李文達 | zh_TW |
dc.date.accessioned | 2021-05-20T21:31:56Z | - |
dc.date.available | 2011-08-19 | |
dc.date.available | 2021-05-20T21:31:56Z | - |
dc.date.copyright | 2010-08-19 | |
dc.date.issued | 2010 | |
dc.date.submitted | 2010-08-18 | |
dc.identifier.citation | Alinovi, C.A., Ward, M.P., Lin, T.L., Moore, G.E., Wu, C.C., 2009, Real-time PCR, compared to liquid and solid culture media and ELISA, for the detection of Mycobacterium avium ssp. paratuberculosis. Vet Microbiol 136, 177-179.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10468 | - |
dc.description.abstract | Bovine paratuberculosis is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The aims of this study was to investigate the current MAP infection status and MAP transmission risk factors in Taiwan; thereby, to develop a practical control program. A nation-wise survey by serum enzyme linked immunosorbent assay (ELISA) was conducted. Environmental samples from dairy herds were collected for detecting MAP by real time PCR and for phylogenic analysis by variable number tandem repeats (VNTRs). Results indicated that MAP seroprevalence in dairy cow was 10.1%, and 66.7% dairy herds were positive, MAP seroprevalence of beef cattle was 2.8%, MAP seroprevalence of intradermal tuberculin test (ITT) positive cattle was 51.4%. MAP infection status and transmission risk factors are different among different herds. Soil and feces were high risk carries for transmitting MAP. Nine substrains of MAPs were identified indicating direct link of MAP transmission among herds. According to the results above and previous research, a MAP control program was developed. | en |
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dc.description.tableofcontents | 口試委員會審定書...........................................................................................................I
Acknowledgements..........................................................................................................II 中文摘要.................................................................................................................III Abstract..................................................................................................................IV Figures....................................................................................................................V Tables....................................................................................................................VI Chapter I: Literature review...............................................................................................1 Etiology of bovine paratuberculosis........................................................................................1 Influences of bovine paratuberculosis…....................................................................................1 Transmission pathways and risk factors of bovine paratuberculosis..........................................................6 Diagnosis of bovine paratuberculosis......................................................................................10 Molecular epidemiology of bovine paratuberculosis.........................................................................12 Control and prevention of bovine paratuberculosis.........................................................................14 Bovine paratuberculosis in Taiwan.........................................................................................19 Chapter II: Specific Aim and Hypothesis...................................................................................23 Specific aim..............................................................................................................23 Hypothesis................................................................................................................23 Chapter III: Material and methods.........................................................................................25 Bovine paratuberculosis surveillance......................................................................................25 Collection of bovine serum samples........................................................................................25 Detect the MAP specific antibody for bovine serum samples.................................................................25 Descriptive statistical analysis..........................................................................................26 Spatial Statistical analysis..............................................................................................26 Transmission Risk Factor Analysis for Bovine Paratuberculosis.............................................................27 Collection and pretreatment of environmental samples......................................................................27 Real time PCR of environmental samples....................................................................................28 Molecular epidemiology of bovine paratuberculosis.........................................................................32 Evidence-based control strategy for bovine paratuberculosis in Taiwan.....................................................38 Chapter IV: Results.......................................................................................................40 Bovine paratuberculosis surveillance......................................................................................40 Collection of bovine serum samples........................................................................................40 MAP seroprevalence of dairy herds in Taiwan...............................................................................40 MAP seroprevalence of beef cattle in Taiwan...............................................................................41 MAP seroprevalence of ITT positive cattle in Taiwan.......................................................................41 bPTB spatial analysis of dairy herds in Taiwan............................................................................41 Transmission Risk Factor Analysis for Bovine Paratuberculosis.............................................................48 Environmental samples for IS900 real time PCR.............................................................................48 IS900 Real time PCR of environmental samples..............................................................................48 Environmental samples for molecular epidemiology..........................................................................49 Molecular epidemiology of bovine paratuberculosis by VNTRs................................................................49 Evidence-based control strategy for bovine paratuberculosis in Taiwan.....................................................63 The information about bPTB should be propagandized to livestock owners....................................................63 The most cost-effective diagnostic method to identify the MAP infection status of dairy herd..............................64 The preventive management practices to control bPTB.......................................................................65 The most cost-effective diagnostic method to identify the subclinical infected cattle.....................................66 The optimal method for certification and monitoring scheme of “MAP low-risk”herd........................................67 Chapter V: Discussion.....................................................................................................68 Bovine paratuberculosis surveillance......................................................................................68 The MAP seroprevalence of dairy cattle is 10.1%...........................................................................68 The herd-level MAP prevalence is 66.7%....................................................................................68 The seroprevalences of individual herd are ranged from 0% to 60%..........................................................68 The seroprevalence and herd-level prevalence of bPTB in each country and city.............................................69 The MAP seroprevalence of beef cattle is 2.8%.............................................................................70 The MAP seroprevalence of ITT positive cattle.............................................................................70 The kernel density map of MAP positive dairy herds........................................................................72 Transmission Risk Factor Analysis for Bovine Paratuberculosis.............................................................73 The prevalences of soil and feces samples from MAP-positive herds.........................................................73 The prevalence of water samples from MAP-positive herds...................................................................73 Nine substrains (From A to I) of MAPs were identified.....................................................................74 The Phylogenetic tree of 9 MAP substrains.................................................................................74 The Phylogenetic tree of 9 MAP substrains.................................................................................75 The distributions of MAP substrains in each herd..........................................................................75 Evidence-based control strategy for bovine paratuberculosis in Taiwan.....................................................77 Livestock owner education and propaganda of bPTB..........................................................................77 Identify the MAP status of voluntary dairy herds..........................................................................79 The control and test strategy for MAP positive herds......................................................................80 “MAP low-risk” certification and monitoring program.....................................................................83 Chapter VI: Conclusion....................................................................................................88 Reference.................................................................................................................89 Appendix I...............................................................................................................108 Appendix II..............................................................................................................131 | |
dc.language.iso | en | |
dc.title | 台灣牛隻副結核病的傳播風險因子和控制策略 | zh_TW |
dc.title | Transmission Risk Factor and Control Strategy of Bovine Paratuberculosis in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 98-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 鄭謙仁,龐飛 | |
dc.contributor.oralexamcommittee | 張志成,劉涓 | |
dc.subject.keyword | 控制計畫,血清酵素結合免疫吸附法,副結核病,即時定量聚合,監測,異數重複序列, | zh_TW |
dc.subject.keyword | control program,ELISA,paratuberculosis,real-time PCR,survey,VNTRs, | en |
dc.relation.page | 143 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2010-08-18 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
Appears in Collections: | 獸醫學系 |
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